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公开(公告)号：US20130005000A1
公开(公告)日：2013-01-03
申请号：US13569264
申请日：2012-08-08
申请人： Hidetaka Doi , Yusuke Hagiwara , Yoshihiro Usuda
发明人： Hidetaka Doi , Yusuke Hagiwara , Yoshihiro Usuda
CPC分类号： C12P13/04 , C07K14/24 , C07K14/245 , C12P13/08
摘要： A method for efficiently producing an L-amino acid utilizing a bacterium belonging to the family Enterobacteriaceae from a fatty acid or an alcohol such as glycerol as a raw material is provided. A bacterium belonging to the family Enterobacteriaceae which is able to produce L-amino acid and harbors an RpsA protein which has a mutation such that the native aspartic acid residue at position 210 is replaced with another amino acid residue is used. This bacterium is cultured in a medium containing a carbon source selected from a fatty acid and an alcohol, and the produced L-amino acid is collected from the medium.
摘要翻译： 提供利用脂肪酸或醇如甘油作为原料的属于肠杆菌科的细菌有效生产L-氨基酸的方法。 使用属于肠杆菌科的细菌，其能够产生L-氨基酸并且含有具有突变的RpsA蛋白，使得第210位的天然天冬氨酸残基被另一个氨基酸残基替代。 该细菌在含有选自脂肪酸和醇的碳源的培养基中培养，从培养基中收集产生的L-氨基酸。
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公开(公告)号：US20120219995A1
公开(公告)日：2012-08-30
申请号：US13404148
申请日：2012-02-24
申请人： Hidetaka Doi , Yoshihiro Usuda
发明人： Hidetaka Doi , Yoshihiro Usuda
CPC分类号： C12P13/08 , C12N9/0008 , C12N9/1029 , C12N9/1096 , C12N9/78 , C12N9/80 , C12P13/12 , C12P13/227 , C12Y203/01109 , C12Y206/01081 , C12Y305/01096 , C12Y305/03023
摘要： A method for producing an L-amino acid which includes the steps of culturing a bacterium belonging to the family Enterobacteriaceae and having an L-amino acid producing ability in a medium to produce and accumulate an L-amino acid in the medium, and collecting the L-amino acid from the medium, wherein the bacterium has been modified so that an activity or activities of one or two or more enzymes of the arginine succinyltransferase pathway, such as arginine succinyltransferase, succinylarginine dihydrolase, succinylornithine aminotransferase, succinylglutamate-semialdehyde dehydrogenase, and succinylglutamate desuccinylase, is/are decreased.
摘要翻译： 一种L-氨基酸的制造方法，其特征在于，在培养基中培养属于肠杆菌科并具有L-氨基酸生产能力的细菌的步骤，在培养基中生成并积累L-氨基酸，并收集 来自培养基的L-氨基酸，其中所述细菌已被修饰，使得精氨酸琥珀酰转移酶途径的一种或两种或多种酶的活性或活性，例如精氨酸琥珀酰转移酶，琥珀酰精氨酸二水解酶，琥珀酰鸟氨酸氨基转移酶，琥珀酰谷氨酸 - 半醛脱氢酶和 琥珀酰谷氨酸脱琥珀酰基酶降低。
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公开(公告)号：US08975045B2
公开(公告)日：2015-03-10
申请号：US13569264
申请日：2012-08-08
申请人： Hidetaka Doi , Yusuke Hagiwara , Yoshihiro Usuda
发明人： Hidetaka Doi , Yusuke Hagiwara , Yoshihiro Usuda
CPC分类号： C12P13/04 , C07K14/24 , C07K14/245 , C12P13/08
摘要： A method for efficiently producing an L-amino acid utilizing a bacterium belonging to the family Enterobacteriaceae from a fatty acid or an alcohol such as glycerol as a raw material is provided. A bacterium belonging to the family Enterobacteriaceae which is able to produce L-amino acid and harbors an RpsA protein which has a mutation such that the native aspartic acid residue at position 210 is replaced with another amino acid residue is used. This bacterium is cultured in a medium containing a carbon source selected from a fatty acid and an alcohol, and the produced L-amino acid is collected from the medium.
摘要翻译： 提供利用脂肪酸或醇如甘油作为原料的属于肠杆菌科的细菌有效生产L-氨基酸的方法。 使用属于肠杆菌科的细菌，其能够产生L-氨基酸并且含有具有突变的RpsA蛋白，使得第210位的天然天冬氨酸残基被另一个氨基酸残基替代。 该细菌在含有选自脂肪酸和醇的碳源的培养基中培养，从培养基中收集产生的L-氨基酸。
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公开(公告)号：US08389249B2
公开(公告)日：2013-03-05
申请号：US12951187
申请日：2010-11-22
IPC分类号： C12P13/04
CPC分类号： C12N15/52 , C12P13/04 , C12P13/08 , C12P13/227
摘要： An L-amino acid can be produced by culturing a bacterium which belongs to the Enterobacteriaceae family, and has an enhanced ability to use a fatty acid. The bacterium is capable of producing the L-amino acid in a culture medium containing a fatty acid or a hydrolysate of an oil-and-fat as a carbon source, thereby producing and accumulating the L-amino acid in a culture.
摘要翻译： L-氨基酸可以通过培养属于肠杆菌科的细菌而具有增强的使用脂肪酸的能力。 该细菌能够在含有脂肪酸或油脂脂肪的水解物作为碳源的培养基中产生L-氨基酸，从而在培养物中产生和积累L-氨基酸。
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5.发明授权Method for producing an L-amino acid in a cultured bacterium having reduced activity of the arginine succinyltransferase pathway 有权在精氨酸琥珀酰转移酶途径活性降低的培养细菌中生产L-氨基酸的方法公开(公告)号：US08932834B2
公开(公告)日：2015-01-13
申请号：US13404148
申请日：2012-02-24
申请人： Hidetaka Doi , Yoshihiro Usuda
发明人： Hidetaka Doi , Yoshihiro Usuda
CPC分类号： C12P13/08 , C12N9/0008 , C12N9/1029 , C12N9/1096 , C12N9/78 , C12N9/80 , C12P13/12 , C12P13/227 , C12Y203/01109 , C12Y206/01081 , C12Y305/01096 , C12Y305/03023
摘要： A method for producing an L-amino acid which includes the steps of culturing a bacterium belonging to the family Enterobacteriaceae and having an L-amino acid producing ability in a medium to produce and accumulate an L-amino acid in the medium, and collecting the L-amino acid from the medium, wherein the bacterium has been modified so that an activity or activities of one or two or more enzymes of the arginine succinyltransferase pathway, such as arginine succinyltransferase, succinylarginine dihydrolase, succinylornithine aminotransferase, succinylglutamate-semialdehyde dehydrogenase, and succinylglutamate desuccinylase, is/are decreased.
摘要翻译： 一种L-氨基酸的制造方法，其特征在于，在培养基中培养属于肠杆菌科并具有L-氨基酸生产能力的细菌的步骤，在培养基中生成并积累L-氨基酸，并收集 来自培养基的L-氨基酸，其中所述细菌已被修饰，使得精氨酸琥珀酰转移酶途径的一种或两种或多种酶的活性或活性，例如精氨酸琥珀酰转移酶，琥珀酰精氨酸二水解酶，琥珀酰鸟氨酸氨基转移酶，琥珀酰谷氨酸 - 半醛脱氢酶和 琥珀酰谷氨酸脱琥珀酰基酶降低。
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公开(公告)号：US20110117613A1
公开(公告)日：2011-05-19
申请号：US12951187
申请日：2010-11-22
CPC分类号： C12N15/52 , C12P13/04 , C12P13/08 , C12P13/227
摘要： An L-amino acid can be produced by culturing a bacterium which belongs to the Enterobacteriaceae family, and has an enhanced ability to use a fatty acid. The bacterium is capable of producing the L-amino acid in a culture medium containing a fatty acid or a hydrolysate of an oil-and-fat as a carbon source, thereby producing and accumulating the L-amino acid in a culture.
摘要翻译： L-氨基酸可以通过培养属于肠杆菌科的细菌而具有增强的使用脂肪酸的能力。 该细菌能够在含有脂肪酸或油脂脂肪的水解物作为碳源的培养基中产生L-氨基酸，从而在培养物中产生和积累L-氨基酸。
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