公开(公告)号：US07462324B2

公开(公告)日：2008-12-09

申请号：US10495621

申请日：2002-11-19

申请人： Nobuhiko Ozaki ,  Hiroaki Oka ,  Hirokazu Sugihara

发明人： Nobuhiko Ozaki ,  Hiroaki Oka ,  Hirokazu Sugihara

IPC分类号： G01N27/00 ,  G01N15/06 ,  G01N33/53 ,  G01N25/18

CPC分类号： G01N33/48707 ,  B01L3/5027

摘要： A measurement device 10 includes a measurement cell 11. The measurement cell 11 includes a substrates 17 and a substrate 16 formed on the substrate 17. The substrates 16 and 17 are bonded to each other. Thus, a flow pass 9 is formed in the measurement cell 11. As shown in FIG. 3, on the substrate 17 of the measurement cell 11, provided are a concave well 19, a protruding microelectrode 1 connected to a terminal 4a and formed on a side wall surface of the well 19, communicating tubes 15a and 15b connected to both of side surfaces of the well 19 (i.e., the flow pass 9) to face to each other, resistance baths 6a and 6b connected to the communicating tubes 15a and 15b, thin film resistors 3a and 3b arranged in the resistance baths 6a and 6b, and a reference electrode 2 connected to a terminal 4b.

摘要翻译： 测量装置10包括测量单元11.测量单元11包括基板17和形成在基板17上的基板16.基板16和17彼此接合。 因此，在测量单元11中形成流路9。 如图3所示，在测量单元11的基板17上设置凹槽19，连接到端子4a并形成在井19的侧壁表面上的突出微电极1，连接到两侧的连通管15a和15b 孔19的表面（即，流路9）彼此相对，连接到连通管15a和15b的电阻槽6a和6b，布置在电阻槽6a和6b中的薄膜电阻器3a和3b，以及 参考电极2连接到端子4b。

公开(公告)号：US07006929B2

公开(公告)日：2006-02-28

申请号：US10479938

申请日：2002-06-05

申请人： Hiroaki Oka ,  Nobuhiko Ozaki ,  Hirokazu Sugihara ,  Satoshi Konishi

发明人： Hiroaki Oka ,  Nobuhiko Ozaki ,  Hirokazu Sugihara ,  Satoshi Konishi

IPC分类号： G06F19/00

CPC分类号： G01N33/48728

摘要： A sensor capable of simply and quickly measuring the electrical activities of a number of cells simultaneously and a device comprising the sensor and capable of detecting the signal transmission between subject cells are provided. The sensor comprises a plurality of electrodes provided on a substrate. The electrodes individually measure a signal generated by a biological sample held by each of the electrodes. Each of the electrodes comprises a depression for holding the biological sample, a throughhole which is in communication with the depression and penetrates to a rear side of the substrate, an electrode portion, and a drawing line from the electrode portion. The electrodes are arranged so that the samples held by the depressions electrically communicate with one another.

摘要翻译： 提供了能够简单且快速地同时测量多个单元的电活动的传感器，以及包括该传感器并且能够检测主体单元之间的信号传输的设备。 传感器包括设置在基板上的多个电极。 电极单独地测量由每个电极保持的生物样品产生的信号。 每个电极包括用于保持生物样品的凹陷，与凹陷连通并穿透到基底的后侧的通孔，电极部分和来自电极部分的引出线。 电极被布置成使得由凹陷保持的样品彼此电连通。

公开(公告)号：US07594984B2

公开(公告)日：2009-09-29

申请号：US11652605

申请日：2007-01-12

申请人： Hiroaki Oka ,  Nobuhiko Ozaki ,  Hirokazu Sugihara

发明人： Hiroaki Oka ,  Nobuhiko Ozaki ,  Hirokazu Sugihara

IPC分类号： G01N33/50 ,  G01N27/00 ,  G01N33/543 ,  C12Q1/00 ,  G01N17/04

CPC分类号： G01N33/48728 ,  G01N33/54373 ,  G01N33/5438

摘要： The present invention provides an apparatus for measuring activity signals of a biological sample comprising: a measurement chamber (A) storing a target liquid containing a biological sample; a porous insulating substrate (5) provided with a measurement electrode (1) on at least one side; and a conveying device (8) which conveys the target liquid stored in the measurement chamber (A) and passes the target liquid through the porous insulating substrate (5) from the measurement electrode (1) side, wherein the conveying device (8) is operated to trap the biological sample contained in the target liquid onto the measurement electrode (1), so that the activity signals of the biological sample are measured through the measurement electrode (1). According to the apparatus, activity signals emitted from the biological sample can be detected easily, rapidly and accurately.

摘要翻译： 本发明提供了一种用于测量生物样品的活动信号的装置，包括：存储含有生物样品的目标液体的测量室（A） 在至少一侧设置有测量电极（1）的多孔绝缘基板（5） 以及输送装置（8），其输送存储在测量室（A）中的目标液体，并将目标液体通过多孔绝缘基板（5）从测量电极（1）侧移动，其中输送装置（8）为 操作以将包含在目标液体中的生物样品捕获到测量电极（1）上，从而通过测量电极（1）测量生物样品的活性信号。 根据该装置，可以容易，快速且准确地检测从生物样品发射的活动信号。

公开(公告)号：US07172860B2

公开(公告)日：2007-02-06

申请号：US10678138

申请日：2003-10-06

申请人： Hiroaki Oka ,  Nobuhiko Ozaki ,  Hirokazu Sugihara

发明人： Hiroaki Oka ,  Nobuhiko Ozaki ,  Hirokazu Sugihara

IPC分类号： C12Q1/00

CPC分类号： G01N33/48728 ,  G01N33/54373 ,  G01N33/5438

摘要： The present invention provides an apparatus for measuring activity signals of a biological sample comprising: a measurement chamber (A) storing a target liquid containing a biological sample; a porous insulating substrate (5) provided with a measurement electrode (1) on at least one side; and a conveying device (8) which conveys the target liquid stored in the measurement chamber (A) and passes the target liquid through the porous insulating substrate (5) from the measurement electrode (1) side, wherein the conveying device (8) is operated to trap the biological sample contained in the target liquid onto the measurement electrode (1), so that the activity signals of the biological sample are measured through the measurement electrode (1). According to the apparatus, activity signals emitted from the biological sample can be detected easily, rapidly and accurately.

摘要翻译： 本发明提供了一种用于测量生物样品的活动信号的装置，包括：存储含有生物样品的目标液体的测量室（A） 在至少一侧设置有测量电极（1）的多孔绝缘基板（5） 以及输送装置（8），其输送存储在测量室（A）中的目标液体，并将目标液体通过多孔绝缘基板（5）从测量电极（1）侧移动，其中输送装置（8）为 操作以将包含在目标液体中的生物样品捕获到测量电极（1）上，从而通过测量电极（1）测量生物样品的活性信号。 根据该装置，可以容易，快速且准确地检测从生物样品发射的活动信号。

公开(公告)号：US06999905B2

公开(公告)日：2006-02-14

申请号：US10296007

申请日：2002-04-26

申请人： Ryuta Ogawa ,  Hiroaki Oka ,  Nobuhiko Ozaki ,  Hirokazu Sugihara

发明人： Ryuta Ogawa ,  Hiroaki Oka ,  Nobuhiko Ozaki ,  Hirokazu Sugihara

IPC分类号： G06F15/00

CPC分类号： G01D1/14

摘要： A method for extracting a signal of interest from a signal generated from at least one subject, comprises the steps of (A) obtaining a time-series signal generated from the subject as a data group consisting of a plurality of pieces of data, (B) obtaining a plurality of extracted data groups from the data group, wherein each extracted data group comprises a predetermined number of pieces of data selected from the data group, (C) calculating standard deviations of the plurality of extracted data groups to obtain a standard deviation group, and (D) referencing each standard deviation included in the standard deviation group and selecting the signal of interest.

摘要翻译： 一种用于从至少一个对象产生的信号中提取感兴趣的信号的方法，包括以下步骤：（A）获得从被摄体产生的时间序列信号作为由多条数据组成的数据组（B ）从所述数据组中获取多个提取的数据组，其中每个提取的数据组包括从所述数据组中选择的预定数量的数据，（C）计算所述多个提取的数据组的标准偏差以获得标准偏差 组和（D）参考包括在标准偏差组中的每个标准偏差并选择感兴趣的信号。

公开(公告)号：US20050004442A1

公开(公告)日：2005-01-06

申请号：US10495621

申请日：2002-11-19

申请人： Nobuhiko Ozaki ,  Hiroaki Oka ,  Hirokazu Sugihara

发明人： Nobuhiko Ozaki ,  Hiroaki Oka ,  Hirokazu Sugihara

IPC分类号： B01L3/00 ,  G01N33/487 ,  A61B5/05

CPC分类号： G01N33/48707 ,  B01L3/5027

摘要： A measurement device 10 includes a measurement cell 11. The measurement cell 11 includes a substrates 17 and a substrate 16 formed on the substrate 17. The substrates 16 and 17 are bonded to each other. Thus, a flow pass 9 is formed in the measurement cell 11. As shown in FIG. 3, on the substrate 17 of the measurement cell 11, provided are a concave well 19, a protruding microelectrode 1 connected to a terminal 4a and formed on a side wall surface of the well 19, communicating tubes 15a and 15b connected to both of side surfaces of the well 19 (i.e., the flow pass 9) to face to each other, resistance baths 6a and 6b connected to the communicating tubes 15a and 15b, thin film resistors 3a and 3b arranged in the resistance baths 6a and 6b, and a reference electrode 2 connected to a terminal 4b.

摘要翻译： 测量装置10包括测量单元11.测量单元11包括基板17和形成在基板17上的基板16.基板16和17彼此接合。 因此，在测量单元11中形成流路9。 如图3所示，在测量单元11的基板17上设置凹槽19，连接到端子4a并形成在井19的侧壁表面上的突出微电极1，连接到两侧的连通管15a和15b 孔19的表面（即，流路9）彼此相对，连接到连通管15a和15b的电阻槽6a和6b，布置在电阻槽6a和6b中的薄膜电阻器3a和3b，以及 参考电极2连接到端子4b。

公开(公告)号：US07326384B2

公开(公告)日：2008-02-05

申请号：US10208547

申请日：2002-07-29

申请人： Hiroaki Oka ,  Nobuhiko Ozaki ,  Hirokazu Sugihara

发明人： Hiroaki Oka ,  Nobuhiko Ozaki ,  Hirokazu Sugihara

IPC分类号： B32B5/02

CPC分类号： G01N33/48728

摘要： A device for detecting a physicochemical change in a biological sample comprising at least one measuring electrode provided by causing a conductive material to enter into a porous film, and a conductor connected to the measuring electrode.

摘要翻译： 一种用于检测生物样品中物理化学变化的装置，包括通过使导电材料进入多孔膜而提供的至少一个测量电极和连接到测量电极的导体。

公开(公告)号：US20070134651A1

公开(公告)日：2007-06-14

申请号：US11652605

申请日：2007-01-12

申请人： Hiroaki Oka ,  Nobuhiko Ozaki ,  Hirokazu Sugihara

发明人： Hiroaki Oka ,  Nobuhiko Ozaki ,  Hirokazu Sugihara

IPC分类号： C12Q1/00 ,  G06F19/00

CPC分类号： G01N33/48728 ,  G01N33/54373 ,  G01N33/5438

摘要： The present invention provides an apparatus for measuring activity signals of a biological sample comprising: a measurement chamber (A) storing a target liquid containing a biological sample; a porous insulating substrate (5) provided with a measurement electrode (1) on at least one side; and a conveying device (8) which conveys the target liquid stored in the measurement chamber (A) and passes the target liquid through the porous insulating substrate (5) from the measurement electrode (1) side, wherein the conveying device (8) is operated to trap the biological sample contained in the target liquid onto the measurement electrode (1), so that the activity signals of the biological sample are measured through the measurement electrode (1). According to the apparatus, activity signals emitted from the biological sample can be detected easily, rapidly and accurately.

公开(公告)号：US07041492B2

公开(公告)日：2006-05-09

申请号：US10048662

申请日：2001-06-20

申请人： Hiroaki Oka ,  Tetsuo Yukimasa ,  Ryuta Ogawa ,  Hirokazu Sugihara ,  Katsuyuki Tsuji ,  Yukifumi Yoshimoto

发明人： Hiroaki Oka ,  Tetsuo Yukimasa ,  Ryuta Ogawa ,  Hirokazu Sugihara ,  Katsuyuki Tsuji ,  Yukifumi Yoshimoto

IPC分类号： C12M1/42

CPC分类号： A61N1/0492 ,  A61N1/0476 ,  A61N1/0551 ,  G01N33/4836

摘要： A multiple electrode includes a plurality of micro-electrodes provided on a substrate, and a wiring portion for providing an electrical signal to the micro-electrodes or extracting an electrical signal from the micro-electrodes. Each micro-electrode has porous conductive material on its surface, and the impedance of the micro-electrode is 50 kΩ or less. Preferably, the porous conductive material is gold, and formed by the passage of current at a current density of 1.0 to 5.0 A/dm2 for 10 to 360 sec. The multiple electrode may include micro-electrodes provided on a substrate in the form of a matrix, a lead line connected to the micro-electrodes, and an electrical junction connected to an end of the lead line.

摘要翻译： 多个电极包括设置在基板上的多个微电极和用于向微电极提供电信号或从微电极提取电信号的布线部分。 每个微电极在其表面上具有多孔导电材料，微电极的阻抗为50kΩ以下。 优选地，多孔导电材料是金，并且通过电流以1.0至5.0A / dm 2的电流通过10至360秒形成。 多个电极可以包括以矩阵形式设置在基板上的微电极，连接到微电极的引线和连接到引线的端部的电连接。

公开(公告)号：US06894511B2

公开(公告)日：2005-05-17

申请号：US10019417

申请日：2001-07-12

申请人： Tetsuo Yukimasa ,  Hiroaki Oka ,  Ryuta Ogawa ,  Hirokazu Sugihara

发明人： Tetsuo Yukimasa ,  Hiroaki Oka ,  Ryuta Ogawa ,  Hirokazu Sugihara

IPC分类号： G01N33/483 ,  C12M1/00 ,  G01N27/30 ,  G01N27/403 ,  G01N33/487 ,  G01R27/08

CPC分类号： G01N33/48728

摘要： A multiple electrode for measuring electro-physiological characteristics of a biological specimen includes a plurality of micro-electrode provided on a first region on a substrate, and a reference electrode provided in a second region on the substrate. The reference electrode includes at least one stimulus reference electrode for applying an electrical signal to the plurality of micro-electrodes. Preferably, the reference electrode includes at least one measurement reference electrode for detecting an electrical signal from the plurality of micro-electrodes, and the stimulus reference electrode is electrically insulated from the measurement reference electrode. Preferably, the second region is placed at a distance from an outer edge of the first region, and surrounds the first region.

摘要翻译： 用于测量生物样品的电生理特性的多电极包括设置在基板上的第一区域上的多个微电极和设置在基板上的第二区域中的参考电极。 参考电极包括用于向多个微电极施加电信号的至少一个激励参考电极。 优选地，参考电极包括用于检测来自多个微电极的电信号的至少一个测量参考电极，并且刺激参考电极与测量参考电极电绝缘。 优选地，第二区域被放置在距离第一区域的外边缘一定距离处，并且围绕第一区域。