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    Application of fluorescent protein to garden plant
    6.
    发明授权
    Application of fluorescent protein to garden plant 有权
    荧光蛋白在花园植物中的应用

    公开(公告)号：US08203032B2

    公开(公告)日：2012-06-19

    申请号：US12374995

    申请日：2007-07-25

    申请人： Iwao Waga ,  Hiromi Takenaka ,  Shu Muto

    发明人： Iwao Waga ,  Hiromi Takenaka ,  Shu Muto

    IPC分类号： C12N15/82 ,  C12N15/84 ,  C12N15/12

    CPC分类号： C12N15/8212 ,  C07K14/43509

    摘要： The present invention provides a process for generation of a transformed plant capable of emitting fluorescence by introducing a gene encoding a non-plant-derived fluorescent protein into a plant such that the fluorescent protein is recombinantly expressed in the active form of its mature protein in the leaf or petal of the plant, and also provides a transformed garden plant capable of emitting fluorescence that is generated by using the process. For example, cDNA encoding the full-length amino acid sequence of a Chiridius poppei-derived fluorescent protein CpYGFP or its H52F modified protein CpYGFP H52F is inserted into a T-DNA-based expression vector system, which is in turn introduced into the chromosomal DNA of a plant. As a result, the transformed plant thus generated can exhibit fluorescence attributed to these fluorescent proteins and exhibit no substantial difference in the other phenotypes from wild-type one of the plant.

    摘要翻译： 本发明提供一种通过将编码非植物来源的荧光蛋白的基因导入植物而产生能够发射荧光的转化植物的方法，使得荧光蛋白以其成熟蛋白的活性形式重组表达 植物的叶或花瓣，并且还提供能够发射通过使用该过程产生的荧光的转化的花园植物。 例如，将编码Chiridius Poppei衍生的荧光蛋白CpYGFP或其H52F修饰的蛋白质CpYGFP H52F的全长氨基酸序列的cDNA插入到基于T-DNA的表达载体系统中，其进而导入染色体DNA 的植物。 结果，由此产生的转化植物可以表现出归因于这些荧光蛋白的荧光，并且在野生型植物中的其它表型中没有显示实质性差异。

    APPLICATION OF FLUORESCENT PROTEIN TO GARDEN PLANT
    7.
    发明申请
    APPLICATION OF FLUORESCENT PROTEIN TO GARDEN PLANT 有权
    荧光蛋白应用于园艺植物

    公开(公告)号：US20100043104A1

    公开(公告)日：2010-02-18

    申请号：US12374995

    申请日：2007-07-25

    申请人： Iwao Waga ,  Hiromi Takenaka ,  Shu Muto

    发明人： Iwao Waga ,  Hiromi Takenaka ,  Shu Muto

    IPC分类号： C12N15/82

    CPC分类号： C12N15/8212 ,  C07K14/43509

    摘要： The present invention provides a process for generation of a transformed plant capable of emitting fluorescence by introducing a gene encoding a non-plant-derived fluorescent protein into a plant such that the fluorescent protein is recombinantly expressed in the active form of its mature protein in the leaf or petal of the plant, and also provides a transformed garden plant capable of emitting fluorescence that is generated by using the process. For example, cDNA encoding the full-length amino acid sequence of a Chiridius poppei-derived fluorescent protein CpYGFP or its H52F modified protein CpYGFP H52F is inserted into a T-DNA-based expression vector system, which is in turn introduced into the chromosomal DNA of a plant. As a result, the transformed plant thus generated can exhibit fluorescence attributed to these fluorescent proteins and exhibit no substantial difference in the other phenotypes from wild-type one of the plant.

    摘要翻译： 本发明提供一种通过将编码非植物来源的荧光蛋白的基因导入植物而产生能够发射荧光的转化植物的方法，使得荧光蛋白以其成熟蛋白的活性形式重组表达 植物的叶或花瓣，并且还提供能够发射通过使用该过程产生的荧光的转化的花园植物。 例如，将编码Chiridius Poppei衍生的荧光蛋白CpYGFP或其H52F修饰的蛋白质CpYGFP H52F的全长氨基酸序列的cDNA插入到基于T-DNA的表达载体系统中，其进而导入染色体DNA 的植物。 结果，由此产生的转化植物可以表现出归因于这些荧光蛋白的荧光，并且在野生型植物中的其它表型中没有显示实质性差异。

    GENE ENCODING NOVEL LUCIFERASE
    9.
    发明申请
    GENE ENCODING NOVEL LUCIFERASE 有权

    公开(公告)号：US20090233320A1

    公开(公告)日：2009-09-17

    申请号：US11721032

    申请日：2004-12-09

    申请人： Hiromi Takenaka

    发明人： Hiromi Takenaka

    IPC分类号： C12Q1/66 ,  C12N15/11

    CPC分类号： C12N9/0069 ,  G01N2333/90241

    摘要： The present invention provides genes encoding novel luciferases having at least the properties of: being capable of using coelenterazine as their luminescent substrates; and being capable of being recombinantly expressed in a mammal cell as a host and produced to be secreted to the outside of the host cell. Specifically, the gene encoding novel luciferases according to the present invention is a DNA molecule comprising a nucleotide sequence encoding any of the full-length amino acid sequences of two types of luciferase proteins, luciferase 1 and luciferase 2, from M. pacifica, and is, for example, a gene encoding the following full-length amino acid sequence of the luciferase 1. MMEIQVLFAL ICFALVQANP TENKDDIDIV GVEGKFGTTD60 LETDLFTIVE DMNVISRDTN LANSDADRGK MPGKKLPLEV LIEMEANARK AGCTRGCLIC120 LSKIKCTAKM KVYIPGRCHD YGGDKKTGQA GIVGAIVDIP EISGFKELGP MEQFIAQVDL180 CADCTTGCLK GLANVKCSAL LKKWLPDRCA SFADKIQSEV DNIKGLAGDR210