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    Mutant α-amylases
    3.
    发明授权
    Mutant α-amylases 失效
    突变型α-淀粉酶

    公开(公告)号：US07078212B1

    公开(公告)日：2006-07-18

    申请号：US09590375

    申请日：2000-06-09

    申请人： Keiji Endo ,  Kazuaki Igarashi ,  Yasuhiro Hayashi ,  Hiroshi Hagihara ,  Katsuya Ozaki

    发明人： Keiji Endo ,  Kazuaki Igarashi ,  Yasuhiro Hayashi ,  Hiroshi Hagihara ,  Katsuya Ozaki

    IPC分类号： C12N9/26 ,  C12N9/28 ,  C07H21/04

    CPC分类号： C12N9/2417 ,  C11D3/386

    摘要： The invention relates to a mutant α-amylase obtained by making replacement or deletion of at least one of amino acid residues such as the 167th Gln, 169th Tyr and 178th Ala in the amino acid sequence set forth in SEQ ID NO:1 in an α-amylase having said amino acid sequence, or an α-amylase having a homology of at least 70% to said amino acid sequence, a gene encoding the mutant α-amylase, a vector, transformed cells, a process for producing a mutant α-amylase, comprising culturing the transformed cells, and a detergent composition comprising the mutant α-amylase.The mutant α-amylase of the invention has excellent properties of high resistance to chelating agents, high specific activity in an alkaline region and excellent stability to heat, and is hence useful for detergents for automatic dish washer, laundry detergents and the like.

    摘要翻译： 本发明涉及通过使SEQ ID NO：1所示的氨基酸序列中的至少一个氨基酸残基例如第167位Gln，第169位和第178位氨基酸置换成α位点而获得的突变型α-淀粉酶 具有所述氨基酸序列的淀粉酶或与所述氨基酸序列具有至少70％同源性的α-淀粉酶，编码突变型α-淀粉酶的基因，载体，转化细胞，用于产生突变型α-淀粉酶的方法， 包括培养转化细胞的淀粉酶和包含突变型α-淀粉酶的洗涤剂组合物。 本发明的突变型α-淀粉酶具有优异的抗螯合剂抗性，在碱性区域中具有高比活性和优异的耐热稳定性，因此可用于自动洗碗机，衣物洗涤剂等的洗涤剂。

    Mutant alpha-amylases
    5.
    发明申请
    Mutant alpha-amylases 审中-公开
    突变型α-淀粉酶

    公开(公告)号：US20050181493A1

    公开(公告)日：2005-08-18

    申请号：US10988529

    申请日：2004-11-16

    申请人： Keiji Endo ,  Kazuaki Igarashi ,  Yasuhiro Hayashi ,  Hiroshi Hagihara ,  Katsuya Ozaki

    发明人： Keiji Endo ,  Kazuaki Igarashi ,  Yasuhiro Hayashi ,  Hiroshi Hagihara ,  Katsuya Ozaki

    IPC分类号： C11D3/386 ,  C12N1/21 ,  C12N9/28 ,  C12N15/56 ,  C11D7/42

    CPC分类号： C12N9/2417 ,  C11D3/386

    摘要： The invention relates to a mutant α-amylase obtained by making replacement or deletion of at least one of amino acid residues such as the 167th Gln, 169th Tyr and 178th Ala in the amino acid sequence set forth in SEQ ID NO:1 in an α-amylase having said amino acid sequence, or an α-amylase having a homology of at least 70% to said amino acid sequence, a gene encoding the mutant α-amylase, a vector, transformed cells, a process for producing a mutant α-amylase, comprising culturing the transformed cells, and a detergent composition comprising the mutant α-amylase. The mutant α-amylase of the invention has excellent properties of high resistance to chelating agents, high specific activity in an alkaline region and excellent stability to heat, and is hence useful for detergents for automatic dish washer, laundry detergents and the like.

    摘要翻译： 本发明涉及通过使SEQ ID NO：1所示的氨基酸序列中的至少一个氨基酸残基例如第167位Gln，第169位和第178位氨基酸置换成α位点而获得的突变型α-淀粉酶 具有所述氨基酸序列的淀粉酶或与所述氨基酸序列具有至少70％同源性的α-淀粉酶，编码突变型α-淀粉酶的基因，载体，转化细胞，用于产生突变型α-淀粉酶的方法， 包括培养转化细胞的淀粉酶和包含突变型α-淀粉酶的洗涤剂组合物。 本发明的突变型α-淀粉酶具有优异的抗螯合剂抗性，在碱性区域中具有高比活性和优异的耐热稳定性，因此可用于自动洗碗机，衣物洗涤剂等的洗涤剂。

    MUTANT ALPHA-AMYLASES
    6.
    发明申请
    MUTANT ALPHA-AMYLASES 审中-公开
    突变型ALPHA-AMYLASES

    公开(公告)号：US20090226961A1

    公开(公告)日：2009-09-10

    申请号：US11864245

    申请日：2007-09-28

    申请人： Keiji Endo ,  Kazuaki Igarashi ,  Yasuhiro Hayashi ,  Hiroshi Hagihara ,  Katsuya Ozaki

    发明人： Keiji Endo ,  Kazuaki Igarashi ,  Yasuhiro Hayashi ,  Hiroshi Hagihara ,  Katsuya Ozaki

    IPC分类号： C12P21/00 ,  C07H21/00 ,  C12N15/63 ,  C12N1/00

    CPC分类号： C12N9/2417 ,  C11D3/386

    摘要： The invention relates to a mutant α-amylase obtained by making replacement or deletion of at least one of amino acid residues such as the 167th Gln, 169th Tyr and 178th Ala in the amino acid sequence set forth in SEQ ID NO:1 in an α-amylase having said amino acid sequence, or an α-amylase having a homology of at least 70% to said amino acid sequence, a gene encoding the mutant α-amylase, a vector, transformed cells, a process for producing a mutant α-amylase, comprising culturing the transformed cells, and a detergent composition comprising the mutant α-amylase.The mutant α-amylase of the invention has excellent properties of high resistance to chelating agents, high specific activity in an alkaline region and excellent stability to heat, and is hence useful for detergents for automatic dish washer, laundry detergents and the like.

    摘要翻译： 本发明涉及通过使SEQ ID NO：1所示的氨基酸序列中的至少一个氨基酸残基例如第167位Gln，第169位和第178位氨基酸置换成α位点而获得的突变型α-淀粉酶 具有所述氨基酸序列的淀粉酶，或与所述氨基酸序列具有至少70％同源性的α-淀粉酶，编码突变型α-淀粉酶的基因，载体，转化细胞，产生突变型α-淀粉酶的方法， 包括培养转化细胞的淀粉酶和包含突变型α-淀粉酶的洗涤剂组合物。 本发明的突变型α-淀粉酶具有优异的抗螯合剂抗性，在碱性区域中具有高比活性和优异的耐热稳定性，因此可用于自动洗碗机，衣物洗涤剂等的洗涤剂。

    Mutant &agr;-amylases
    9.
    发明授权
    Mutant &agr;-amylases 失效
    突变型α-淀粉酶

    公开(公告)号：US06486113B1

    公开(公告)日：2002-11-26

    申请号：US09381687

    申请日：1999-09-23

    申请人： Yuji Hatada ,  Kaori Ikawa ,  Susumu Ito ,  Kazuaki Igarashi ,  Hiroshi Hagihara ,  Yasuhiro Hayashi ,  Hiroyuki Araki ,  Katsuya Ozaki

    发明人： Yuji Hatada ,  Kaori Ikawa ,  Susumu Ito ,  Kazuaki Igarashi ,  Hiroshi Hagihara ,  Yasuhiro Hayashi ,  Hiroyuki Araki ,  Katsuya Ozaki

    IPC分类号： C11D3386

    CPC分类号： C12N9/2417 ,  C11D3/386

    摘要： The invention relates to a mutant &agr;-amylase having an amino acid sequence obtained by making deletion or replacement by another arbitrary amino acid residue of at least a methionine residue at the 202-position or a position homologous thereto among amino acid residues set forth in SEQ ID NO:1, which constitute a liquefying alkaline &agr;-amylase, a gene thereof, and a detergent composition comprising the mutant &agr;-amylase. The mutant &agr;-amylase has the optimum pH in an alkaline range, an excellent &agr;-amylase activity, and high and lasting resistance to oxidizing agents, and is hence particularly useful as a component of detergent compositions containing a bleaching agent and an oxidizing agent.

    摘要翻译： 本发明涉及一种具有氨基酸序列的突变型α-淀粉酶，该氨基酸序列是通过在SEQ ID NO：1所示的氨基酸残基之间至少含有202位的甲硫氨酸残基或其同源位置的另外任意氨基酸残基进行缺失或替换而获得的 ID NO：1，其构成液化碱性α-淀粉酶，其基因和包含突变型α-淀粉酶的洗涤剂组合物。 突变型α-淀粉酶在碱性范围内具有最佳pH，优异的α-淀粉酶活性和对氧化剂的高持久耐受性，因此特别可用作含有漂白剂和氧化剂的洗涤剂组合物的组分。

    Gene for enzyme having both alkaline pullulanase and alkaline alpha-amylase activities
    10.
    发明授权
    Gene for enzyme having both alkaline pullulanase and alkaline alpha-amylase activities 失效
    具有碱性支链淀粉酶和碱性α-淀粉酶活性的酶的基因

    公开(公告)号：US06689874B2

    公开(公告)日：2004-02-10

    申请号：US10014436

    申请日：2001-12-14

    申请人： Yuji Hatada ,  Kazuaki Igarashi ,  Katsuya Ozaki ,  Katsutoshi Ara ,  Shuji Kawai ,  Susumu Ito

    发明人： Yuji Hatada ,  Kazuaki Igarashi ,  Katsuya Ozaki ,  Katsutoshi Ara ,  Shuji Kawai ,  Susumu Ito

    IPC分类号： C07H2104

    CPC分类号： C12N9/2457 ,  C12N9/2417 ,  C12N9/2451 ,  C12Y302/01001 ,  C12Y302/01041

    摘要： The present invention provides: a nucleotide sequence encoding alkaline pullulanase and exhibiting both alkaline &agr;-amylase and alkaline pullulanase activity; a nucleotide sequence encoding alkaline &agr;-amylase possessing an amino acid sequence described in SEQ ID NO:3; a nucleotide sequence encoding alkaline pullulanase possessing an amino acid sequence described in SEQ ID NO:4; recombinant DNAs containing these nucleotide sequences, and transformed microorganisms harboring the recombinant DNAs. The technique of the present invention enables mass production of alkaline pullulanase exhibiting both alkaline &agr;-amylase and alkaline pullulanase activity.

    摘要翻译： 本发明提供：编码碱基支链淀粉酶并表现出碱性α-淀粉酶和碱性支链淀粉酶活性的核苷酸序列; 编码具有SEQ ID NO：3所述的氨基酸序列的碱性α-淀粉酶的核苷酸序列; 编码具有SEQ ID NO：4所述的氨基酸序列的碱基支链淀粉酶的核苷酸序列; 含有这些核苷酸序列的重组DNA和携带重组DNA的转化微生物。 本发明的技术能够大量生产表现出碱性α-淀粉酶和碱性支链淀粉酶活性的碱性支链淀粉酶。