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1.发明授权公开(公告)号：US08039248B2
公开(公告)日：2011-10-18
申请号：US12547276
申请日：2009-08-25
CPC分类号： C12N9/0006
摘要： The invention provides a modified flavin adenine dinucleotide dependent glucose dehydrogenase (FADGDH), as well as a glucose sensor comprising the modified FADGDH and a method for measuring glucose comprising using the glucose sensor to measure glucose of a sample.
摘要翻译： 本发明提供了修饰的黄素腺嘌呤二核苷酸依赖性葡萄糖脱氢酶（FADGDH），以及包含修饰的FADGDH的葡萄糖传感器和测量葡萄糖的方法，包括使用葡萄糖传感器测量样品的葡萄糖。
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2.发明授权公开(公告)号：US07662600B2
公开(公告)日：2010-02-16
申请号：US11939348
申请日：2007-11-13
CPC分类号： C12N9/0006
摘要： An object of the present invention is to provide a more practically advantageous enzyme usable as a reagent for measuring blood glucose than the known enzymes used as blood glucose sensors.A modified flavin adenine dinucleotide dependent glucose dehydrogenase (FADGDH) with more improved heat stability than FADGDH derived from wild-type FADGDH, the modified FADGDH being derived from preferably a eukaryote, more preferably a filamentous fungus, and furthermore preferably an Aspergillus fungus, and, for example, those having a primary structure with at least one amino acid substituted, deleted, inserted or added to FADGDH having an amino acid sequence represented by SEQ ID Nos. 2 or 46 in the sequence table.
摘要翻译： 本发明的目的是提供比用作血糖传感器的已知酶更可实用的用作测量血糖的试剂的酶。 与来自野生型FADGDH的FADGDH相比，具有比来自野生型FADGDH的FADGDH更好的热稳定性的改良的黄素腺嘌呤二核苷酸依赖性葡萄糖脱氢酶（FADGDH），修饰的FADGDH优选来自真核生物，更优选丝状真菌，更优选来自真菌， 例如具有至少一个氨基酸被取代，缺失，插入或添加到具有序列表中SEQ ID No.2或46所示的氨基酸序列的FADGDH的一级结构的那些。
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3.发明申请METHOD FOR HIGHLY EXPRESSING RECOMBINANT GLUCOSE DEHYDROGENASE DERIVED FROM FILAMENTOUS BACTERIUM 失效用于高度表达从纤维细菌衍生的重组葡萄糖脱氢酶的方法公开(公告)号：US20080014611A1
公开(公告)日：2008-01-17
申请号：US11692648
申请日：2007-03-28
CPC分类号： C12N9/0006
摘要： The present invention provides a method for highly expressing a recombinant FAD-GDH protein derived from filamentous fungi, protein obtained by the method, and a regent for measuring glucose using the protein. According to the invention, the FAD-GDH can be highly expressed by altering DNA sequence coding for a signal peptide of FAD-GDH gene isolated from Aspergillus oryzae. FAD-GDH can be stably produced by adjusting pH of 7.1 to 7.3 during culture production.
摘要翻译： 本发明提供了高效表达来源于丝状真菌的重组FAD-GDH蛋白，通过该方法得到的蛋白质和使用该蛋白质测定葡萄糖的摄取剂的方法。 根据本发明，通过改变编码从米曲霉分离的FAD-GDH基因的信号肽的DNA序列可以高度表达FAD-GDH。 通过在培养生产过程中调节pH值为7.1〜7.3可以稳定地生产FAD-GDH。
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4.发明授权Method for highly expressing recombinant glucose dehydrogenase derived from filamentous fungi 失效高度表达源自丝状真菌的重组葡萄糖脱氢酶的方法公开(公告)号：US07741100B2
公开(公告)日：2010-06-22
申请号：US11692648
申请日：2007-03-28
CPC分类号： C12N9/0006
摘要： The present invention provides a method for highly expressing a recombinant FAD-GDH protein derived from filamentous fungi, protein obtained by the method, and a regent for measuring glucose using the protein. According to the invention, the FAD-GDH can be highly expressed by altering DNA sequence coding for a signal peptide of FAD-GDH gene isolated from Aspergillus oryzae. FAD-GDH can be stably produced by adjusting pH of 7.1 to 7.3 during culture production.
摘要翻译： 本发明提供了高效表达来源于丝状真菌的重组FAD-GDH蛋白，通过该方法得到的蛋白质和使用该蛋白质测定葡萄糖的摄取剂的方法。 根据本发明，通过改变编码从米曲霉分离的FAD-GDH基因的信号肽的DNA序列可以高度表达FAD-GDH。 通过在培养生产过程中调节pH值为7.1〜7.3可以稳定地生产FAD-GDH。
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5.发明授权公开(公告)号：US08247189B2
公开(公告)日：2012-08-21
申请号：US13421207
申请日：2012-03-15
CPC分类号： C12Q1/006 , C12N9/0006 , C12Q1/32 , C12Q1/54
摘要： Provided is an enzyme that is further advantageous in terms of practical aspects when compared to publicly known enzymes for blood sugar sensors, and that can be used in a blood sugar level measuring reagent A flavin adenine dinucleotide-dependent glucose dehydrogenase that has amino acid sequence including a specific amino acid in an amino acid sequence shown in SEQ ID NO:2 or an amino acid sequence that has 60% homology therewith, and that has an improved temperature dependency.
摘要翻译： 提供了与公知的血糖传感器的酶相比，在实用方面更有利的酶，其可用于具有氨基酸序列的血糖水平测定试剂A黄素腺嘌呤二核苷酸依赖型葡萄糖脱氢酶 SEQ ID NO：2所示的氨基酸序列中的特定氨基酸或与其同源性为60％的氨基酸序列，并且具有改善的温度依赖性。
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公开(公告)号：US20080020426A1
公开(公告)日：2008-01-24
申请号：US11694540
申请日：2007-03-30
CPC分类号： C12N9/0006
摘要： The present invention provides glucose dehydrogenase which is excellent in heat resistance and substrate specificity and is not affected by dissolved oxygen. Specifically, the present invention relates to glucose dehydrogenase characterized by being derived from an eukaryotic organism and keeping 90% or more activity after being treated with heat at 55° C. for 15 minutes in a liquid form compared with the activity before being treated.
摘要翻译： 本发明提供耐热性和底物特异性优异且不受溶解氧影响的葡萄糖脱氢酶。 具体地说，本发明涉及葡萄糖脱氢酶，其特征在于，与处理前的活性相比，在55℃下加热处理15分钟后，与来自真核生物体相比，保持90％以上的活性。
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公开(公告)号：US07871805B2
公开(公告)日：2011-01-18
申请号：US12351177
申请日：2009-01-09
IPC分类号： C12N9/10
CPC分类号： C12N9/0006
摘要： The present invention provides glucose dehydrogenase which is excellent in heat resistance and substrate specificity and is not affected by dissolved oxygen. Specifically, the present invention relates to glucose dehydrogenase characterized by being derived from an eukaryotic organism and keeping 90% or more activity after being treated with heat at 55° C. for 15 minutes in a liquid form compared with the activity before being treated.
摘要翻译： 本发明提供耐热性和底物特异性优异且不受溶解氧影响的葡萄糖脱氢酶。 具体地说，本发明涉及葡萄糖脱氢酶，其特征在于，与处理前的活性相比，在55℃下加热处理15分钟后，与来自真核生物体相比，保持90％以上的活性。
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公开(公告)号：US07494794B2
公开(公告)日：2009-02-24
申请号：US11694540
申请日：2007-03-30
CPC分类号： C12N9/0006
摘要： The present invention provides glucose dehydrogenase which is excellent in heat resistance and substrate specificity and is not affected by dissolved oxygen. Specifically, the present invention relates to glucose dehydrogenase characterized by being derived from an eukaryotic organism and keeping 90% or more activity after being treated with heat at 55° C. for 15 minutes in a liquid form compared with the activity before being treated.
摘要翻译： 本发明提供耐热性和底物特异性优异且不受溶解氧影响的葡萄糖脱氢酶。 具体地说，本发明涉及葡萄糖脱氢酶，其特征在于，与处理前的活性相比，在55℃下加热处理15分钟后，与来自真核生物体相比，保持90％以上的活性。
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