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    Modified flavin adenine dinucleotide dependent glucose dehydrogenase
    1.
    发明授权
    Modified flavin adenine dinucleotide dependent glucose dehydrogenase 有权
    改良黄素腺嘌呤二核苷酸依赖性葡萄糖脱氢酶

    公开(公告)号：US07662600B2

    公开(公告)日：2010-02-16

    申请号：US11939348

    申请日：2007-11-13

    申请人： Hiroshi Kawaminami ,  Yuji Tsuji ,  Masao Kitabayashi ,  Yoshiaki Nishiya

    发明人： Hiroshi Kawaminami ,  Yuji Tsuji ,  Masao Kitabayashi ,  Yoshiaki Nishiya

    IPC分类号： C12N9/04 ,  C12Q1/54 ,  C12P19/34 ,  C12N15/00 ,  C12P21/06 ,  C12N1/20 ,  C07H21/04

    CPC分类号： C12N9/0006

    摘要： An object of the present invention is to provide a more practically advantageous enzyme usable as a reagent for measuring blood glucose than the known enzymes used as blood glucose sensors.A modified flavin adenine dinucleotide dependent glucose dehydrogenase (FADGDH) with more improved heat stability than FADGDH derived from wild-type FADGDH, the modified FADGDH being derived from preferably a eukaryote, more preferably a filamentous fungus, and furthermore preferably an Aspergillus fungus, and, for example, those having a primary structure with at least one amino acid substituted, deleted, inserted or added to FADGDH having an amino acid sequence represented by SEQ ID Nos. 2 or 46 in the sequence table.

    摘要翻译： 本发明的目的是提供比用作血糖传感器的已知酶更可实用的用作测量血糖的试剂的酶。 与来自野生型FADGDH的FADGDH相比，具有比来自野生型FADGDH的FADGDH更好的热稳定性的改良的黄素腺嘌呤二核苷酸依赖性葡萄糖脱氢酶（FADGDH），修饰的FADGDH优选来自真核生物，更优选丝状真菌，更优选来自真菌， 例如具有至少一个氨基酸被取代，缺失，插入或添加到具有序列表中SEQ ID No.2或46所示的氨基酸序列的FADGDH的一级结构的那些。

    Modified flavin adenine dinucleotide-dependent glucose dehydrogenase
    5.
    发明授权
    Modified flavin adenine dinucleotide-dependent glucose dehydrogenase 有权
    改良黄素腺嘌呤二核苷酸依赖性葡萄糖脱氢酶

    公开(公告)号：US08247189B2

    公开(公告)日：2012-08-21

    申请号：US13421207

    申请日：2012-03-15

    申请人： Hiroshi Kawaminami ,  Masao Kitabayashi ,  Yoshiaki Nishiya

    发明人： Hiroshi Kawaminami ,  Masao Kitabayashi ,  Yoshiaki Nishiya

    IPC分类号： C12N15/00 ,  C12N1/20 ,  C12Q1/00 ,  C12Q1/54

    CPC分类号： C12Q1/006 ,  C12N9/0006 ,  C12Q1/32 ,  C12Q1/54

    摘要： Provided is an enzyme that is further advantageous in terms of practical aspects when compared to publicly known enzymes for blood sugar sensors, and that can be used in a blood sugar level measuring reagent A flavin adenine dinucleotide-dependent glucose dehydrogenase that has amino acid sequence including a specific amino acid in an amino acid sequence shown in SEQ ID NO:2 or an amino acid sequence that has 60% homology therewith, and that has an improved temperature dependency.

    摘要翻译： 提供了与公知的血糖传感器的酶相比，在实用方面更有利的酶，其可用于具有氨基酸序列的血糖水平测定试剂A黄素腺嘌呤二核苷酸依赖型葡萄糖脱氢酶 SEQ ID NO：2所示的氨基酸序列中的特定氨基酸或与其同源性为60％的氨基酸序列，并且具有改善的温度依赖性。

    Dna synthesis promoters dna polymerase-associated factors and utilization thereof
    6.
    发明申请
    Dna synthesis promoters dna polymerase-associated factors and utilization thereof 有权
    Dna合成启动子dna聚合酶相关因子及其利用

    公开(公告)号：US20050069887A1

    公开(公告)日：2005-03-31

    申请号：US10495581

    申请日：2002-11-14

    申请人： Masao Kitabayashi ,  Toshihiro Kuroita ,  Yoshikazu Ishida ,  Hideyuki Komatsubara ,  Yoshiaki Nishiya ,  Masanori Oka ,  Yoshihisa Kawamura ,  Tadayuki Imanaka

    发明人： Masao Kitabayashi ,  Toshihiro Kuroita ,  Yoshikazu Ishida ,  Hideyuki Komatsubara ,  Yoshiaki Nishiya ,  Masanori Oka ,  Yoshihisa Kawamura ,  Tadayuki Imanaka

    IPC分类号： C07K14/195 ,  C12N9/12 ,  C12P19/34 ,  C12Q1/68

    CPC分类号： C07K14/195 ,  C12N9/1252 ,  C12Q1/6846 ,  C12Q2527/125 ,  C12Q2521/101

    摘要： A composition for enhancing synthesis of DNA comprising an anion-supplying substance that is effective in promoting DNA synthesis in enzymatic DNA synthesis reactions, in particular a salt of a dicarboxylic acid. Further enhanced DNA synthesis promoting effects can be achieved by using, together with the anion-supplying substance, at least one compound selected from the group consisting of dimethylsulfoxide and compounds represented by the following formula (1): R2—CH2—NR1xHy  (1) wherein R1 is an alkyl group having 1 to 3 carbon atoms, R2 is a substituent selected from the group consisting of the following (a) and (b): (a) oxygen and (b) radicals represented by the formula wherein R4 is methyl, hydrogen or forms a pyrrolidine ring when combined with R1, R5 is —CO2H or —SO3H, and n is an integer from 0 to 2, x is an integer from 1 to 3 and y is an integer from 0 to 2, provided that x plus y equals 3. The present invention further provides a thermostable DNA polymerase-related factor derived from Thermococcus species, which has an activity to promote the DNA synthesis activity of DNA polymerase or bind to DNA polymerase.

    摘要翻译： 一种用于增强DNA合成的组合物，其包含在促进DNA合成反应中有效促进DNA合成的阴离子供给物质，特别是二羧酸的盐。 通过与阴离子供给物质一起使用至少一种选自二甲基亚砜和下式（1）表示的化合物的化合物，可以实现进一步增强的DNA合成促进效果：R 2 -CH 2 -NR <1> xHy（1）其中R 1是具有1至3个碳原子的烷基，R 2是选自以下（a）和（b）的取代基：（a）氧 和（b）由下式表示的基团其中R 4是甲基，氢或当与R 1一起形成吡咯烷环时，R 5是-CO 2 H或-SO 3 H，n是0至 2，x是1至3的整数，y是0至2的整数，条件是x加y等于3.本发明还提供了源自Thermococcus物种的热稳定DNA聚合酶相关因子，其具有活性 促进DNA聚合酶的DNA合成活性或与DNA聚合酶结合。

    METHOD FOR ENHANCING STABILITY OF A COMPOSITION COMPRISING SOLUBLE GLUCOSE DEHYDROGENASE (GDH)
    7.
    发明申请
    METHOD FOR ENHANCING STABILITY OF A COMPOSITION COMPRISING SOLUBLE GLUCOSE DEHYDROGENASE (GDH) 审中-公开
    包含可溶葡萄糖脱氢酶（GDH）的组合物稳定性的方法

    公开(公告)号：US20080003628A1

    公开(公告)日：2008-01-03

    申请号：US11694489

    申请日：2007-03-30

    申请人： Masao Kitabayashi ,  Yuji Tsuji ,  Yoshiaki Nishiya ,  Takahide Kishimoto

    发明人： Masao Kitabayashi ,  Yuji Tsuji ,  Yoshiaki Nishiya ,  Takahide Kishimoto

    IPC分类号： C12N9/96 ,  C12Q1/54

    CPC分类号： C12N9/0006 ,  C12N9/96 ,  C12Q1/54 ,  C12Y101/9901 ,  G01N27/3271

    摘要： The present invention relates to a method for enhancing stability of a composition comprising soluble glucose dehydrogenase (GDH). Soluble GDH is preferably FAD-dependent GDH derived from filamentous fungus, and the best effect is observed in FAD-GDH derived from A. oryzae or FAD-GDH derived from A. terreus. According to the invention, in a composition comprising soluble glucose dehydrogenase (GDH), stability of GDH can be enhanced by coexisting the enzyme with one or more compounds selected from amino acids and sugars which are not substrate of the enzyme, thus expected to enhancing a measurement accuracy of glucose.

    摘要翻译： 本发明涉及提高包含可溶性葡萄糖脱氢酶（GDH）的组合物稳定性的方法。 可溶性GDH优选来自丝状真菌的FAD依赖性GDH，并且在来自A.trreus的米曲霉或FAD-GDH的FAD-GDH中观察到最好的效果。 根据本发明，在包含可溶性葡萄糖脱氢酶（GDH）的组合物中，可以通过将酶与一种或多种选自不是酶底物的氨基酸和糖类的化合物共存来增强GDH的稳定性，从而预期增强 葡萄糖测量精度。

    MODIFIED CREATININE AMIDE HYDROLASE HAVING IMPROVED AFFINITY FOR SUBSTRATE, AND REAGENT COMPOSITION FOR DETERMINATION OF CREATININE
    8.
    发明申请
    MODIFIED CREATININE AMIDE HYDROLASE HAVING IMPROVED AFFINITY FOR SUBSTRATE, AND REAGENT COMPOSITION FOR DETERMINATION OF CREATININE 有权
    具有改善基质改性的改性丙烯酸酰胺水解物，以及用于测定CREATININE的试剂组合物

    公开(公告)号：US20090170145A1

    公开(公告)日：2009-07-02

    申请号：US12297572

    申请日：2007-04-20

    申请人： Rie Nagai ,  Masao Kitabayashi ,  Yoshiaki Nishiya

    发明人： Rie Nagai ,  Masao Kitabayashi ,  Yoshiaki Nishiya

    IPC分类号： C12Q1/34 ,  C12N9/78 ,  C12N15/55 ,  C12N15/70 ,  C12N1/21

    CPC分类号： C12Q1/34 ,  C12N9/80

    摘要： [Problems] To overcome disadvantages of a known creatinine amide hydrolase, and provide a creatinine amide hydrolase having improved affinity for creatinine or having a decreased Km value for creatinine, and also provide a reagent composition for use in the determination of creatinine, which is adapted to an automated analysis apparatus and is excellent in accuracy, preciseness and economic efficiency.[Means for Solving Problems] Disclosed is a modified creatinine amide hydrolase having improved affinity for a substrate compared to an unmodified one. Also disclosed is a creatinine determination reagent comprising the modified creatinine amide hydrolase, a creatinine amidino hydrolase, sarcosine oxidase and a reagent for detection of hydrogen peroxide.

    摘要翻译： 为了克服已知的肌酸酐酰胺水解酶的缺点，提供对肌酸酐具有改善的亲和力或肌酐降低的Km值的肌酐酰胺水解酶，并且还提供用于测定肌酸酐的试剂组合物，其适用于 具有自动化分析装置，精度，准确性和经济性优异。 解决问题的方法公开了与未修饰的肌酸酐酰胺水解酶相比，对底物的亲和性改善。 还公开了包含改性肌酐酰胺水解酶，肌酸酐脒基水解酶，肌氨酸氧化酶和检测过氧化氢的试剂的肌酸酐测定试剂。

    NOVEL PROTEIN HAVING FRUCTOSYL VALYL HISTIDINE OXIDASE ACTIVITY, MODIFIED PROTEIN, AND USE OF THE PROTEIN OR THE MODIFIED PROTEIN
    10.
    发明申请
    NOVEL PROTEIN HAVING FRUCTOSYL VALYL HISTIDINE OXIDASE ACTIVITY, MODIFIED PROTEIN, AND USE OF THE PROTEIN OR THE MODIFIED PROTEIN 有权
    具有纤维蛋白胆固醇氧化酶活性，改良蛋白质的蛋白质和蛋白质或改性蛋白质的使用

    公开(公告)号：US20110195444A1

    公开(公告)日：2011-08-11

    申请号：US13123052

    申请日：2009-10-06

    申请人： Rie Hirao ,  Masao Kitabayashi ,  Yoshiaki Nishiya ,  Hiroki Ishida ,  Yoji Hata

    发明人： Rie Hirao ,  Masao Kitabayashi ,  Yoshiaki Nishiya ,  Hiroki Ishida ,  Yoji Hata

    IPC分类号： C12Q1/26 ,  C12N9/02 ,  C07H21/00 ,  C12N15/85 ,  C12N5/10

    CPC分类号： G01N33/723 ,  C12N9/0022 ,  C12Q1/26

    摘要： The present invention provides: a protein having a fructosyl amino acid oxidase activity which protein is useful for measurement of a glycosylated protein (particularly, glycosylated hemoglobin); a modified protein thereof; and use of the protein or the modified protein. The protein of the present invention is, for example, a fructosyl valyl histidine oxidase derived from Phaeosphaeria nodorum, the fructosyl valyl histidine oxidase having excellent thermal stability and substrate specificity and also having a small Km value to fructosyl valyl histidine. This allows a glycosylated protein measuring reagent to be stored in a long time and measurement accuracy of the glycosylated protein measuring reagent to be improved.

    摘要翻译： 本发明提供：具有果糖基氨基酸氧化酶活性的蛋白质，该蛋白质可用于糖基化蛋白质（特别是糖基化血红蛋白）的测定。 其改性蛋白质; 并使用蛋白质或修饰的蛋白质。 本发明的蛋白质是例如来源于花粉球形的果糖基缬氨酰基组氨酸氧化酶，具有优异的热稳定性和底物特异性的果糖基缬氨酰基组氨酸氧化酶，并且对于果糖基缬氨酰基组氨酸也具有小的Km值。 这样可以长时间地存储糖基化蛋白质测定试剂，改善糖基化蛋白质测定试剂的测定精度。