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公开(公告)号：US20110262998A1
公开(公告)日：2011-10-27
申请号：US13125328
申请日：2009-10-21
CPC分类号： C12N9/6491 , C07K2319/02 , C12P21/02
摘要： A process for preparing an inclusion body-forming protein is provided. A nucleic acid fragment consisting of a nucleotide sequence coding for a modified alkaline phosphatase signal peptide (modified APSP) where leucine at the 13th position in the amino acid sequence shown in SEQ ID NO: 1 is substituted with proline and/or alanine at the 21st position is substituted with the other amino acid, downstream of which nucleotide sequence is bound a nucleotide sequence of a gene of a protein of interest, and a process for preparing an inclusion body-forming protein consisting of the following steps: (1) preparing an expression vector in which a nucleic acid fragment is incorporated consisting of a nucleotide sequence coding for a modified signal peptide downstream of which is bound a nucleotide sequence of a gene of a protein of interest, (2) preparing a host cell transformed with the expression vector that produces an inclusion body-forming protein, and (3) purifying the inclusion body-forming protein from culture obtained by culturing the host cell that produces the inclusion body-forming protein. The above other amino acid is selected from the group consisting of aspartic acid, glutamic acid, lysine, histidine, phenylalanine and tyrosine.
摘要翻译： 提供了一种制备包涵体形成蛋白的方法。 由编码改变的碱性磷酸酶信号肽（修饰的APSP）的核苷酸序列组成的核酸片段，其中在第21位被脯氨酸和/或丙氨酸取代在SEQ ID NO：1所示的氨基酸序列中的第13位的亮氨酸 位置被其他氨基酸取代，其中核苷酸序列的下游与感兴趣蛋白质的基因的核苷酸序列结合，以及由以下步骤组成的包涵体形成蛋白的方法：（1）制备 表达载体，其中掺入核酸片段由编码下游的修饰信号肽的核苷酸序列组成，所述核苷酸序列与感兴趣蛋白质的基因的核苷酸序列结合，（2）制备用表达载体转化的宿主细胞 产生包涵体形成蛋白质，（3）通过培养生成的宿主细胞获得的培养物纯化包涵体形成蛋白质 是包涵体形成蛋白。 上述其它氨基酸选自天冬氨酸，谷氨酸，赖氨酸，组氨酸，苯丙氨酸和酪氨酸。
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公开(公告)号：US08748132B2
公开(公告)日：2014-06-10
申请号：US13125328
申请日：2009-10-21
IPC分类号： C12P21/04
CPC分类号： C12N9/6491 , C07K2319/02 , C12P21/02
摘要： A process for preparing an inclusion body-forming protein is provided. A nucleic acid fragment having a nucleotide sequence coding for a modified alkaline phosphatase signal peptide (modified APSP) where leucine at the 13th position in the amino acid sequence shown in SEQ ID NO: 1 is substituted with proline and/or alanine at the 21st position is substituted with the other amino acid, downstream of which nucleotide sequence is bound a nucleotide sequence of a gene of a protein of interest is also provided.
摘要翻译： 提供了一种制备包涵体形成蛋白的方法。 具有编码改变的碱性磷酸酶信号肽（修饰的APSP）的核苷酸序列的核酸片段，其中在SEQ ID NO：1所示的氨基酸序列中的第13位的亮氨酸被第21位的脯氨酸和/或丙氨酸取代 还提供了另一个氨基酸，其下游的核苷酸序列与目的蛋白质的基因的核苷酸序列结合。
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公开(公告)号：US20070249534A1
公开(公告)日：2007-10-25
申请号：US11707942
申请日：2007-02-20
申请人： Masaki Hirashima , Takeshi Naruse , Hiroaki Maeda , Chikateru Nozaki , Takeshi Goto , Katsuhiko Akiyama , Hidenao Fukushima
发明人： Masaki Hirashima , Takeshi Naruse , Hiroaki Maeda , Chikateru Nozaki , Takeshi Goto , Katsuhiko Akiyama , Hidenao Fukushima
IPC分类号： A61K38/16
CPC分类号： A61K38/1709
摘要： A medicament for treating neurodegenerative diseases, comprising as an active ingredient selenoprotein P and/or a peptide fragment or a series of peptide fragments derived from the C-terminal of selenoprotein P. An excellent medicament for treating neurodegenerative diseases, especially suitable for treating neurodegenerative diseases with ataxia as a principal symptom is provided.
摘要翻译： 一种用于治疗神经变性疾病的药物，其包含作为活性成分的硒蛋白P和/或肽片段或一系列衍生自硒蛋白P的C末端的肽片段。用于治疗神经变性疾病的优异药物，特别适用于治疗神经变性疾病 提供共济失调作为主要症状。
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公开(公告)号：US20050281808A1
公开(公告)日：2005-12-22
申请号：US11185859
申请日：2005-07-21
IPC分类号： A61K38/00 , C07K14/47 , A61K39/395
CPC分类号： C07K14/4747 , A61K38/00
摘要： The present invention provides a peptide fragment or a series of peptide fragments having a cell death-inhibitory activity, having the amino acid sequence consisting of 103 amino acid residues at the C-terminal of selenoprotein P, or having said amino acid sequence with one or several amino acid residues therein being deleted, substituted or added, or having a partial sequence of either of the above amino acid sequences, a medicament for treatment comprising said peptide fragment or a series of peptide fragments, an antibody to said peptide fragment or a series of peptide fragments, and a method for screening a cell death-inhibitory activity using said peptide fragment or a series of peptide fragments. The preferable peptide fragment or a series of peptide fragments of the present invention has the amino acid sequences shown in SEQ ID NO: 1 and/or SEQ ID NO: 2 or has a partial sequence thereof.
摘要翻译： 本发明提供具有细胞死亡抑制活性的肽片段或一系列肽片段，其具有在硒蛋白P的C末端由103个氨基酸残基组成的氨基酸序列，或者具有所述氨基酸序列与一个或多个 其中缺少，取代或添加的几个氨基酸残基，或具有上述氨基酸序列之一的部分序列，包含所述肽片段或一系列肽片段的治疗药物，所述肽片段的抗体或一系列 的肽片段，以及使用所述肽片段或一系列肽片段筛选细胞死亡抑制活性的方法。 本发明优选的肽片段或一系列肽片段具有SEQ ID NO：1和/或SEQ ID NO：2所示的氨基酸序列或其部分序列。
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公开(公告)号：US20050037954A1
公开(公告)日：2005-02-17
申请号：US10472444
申请日：2001-08-31
CPC分类号： A61K38/1709
摘要： A novel medicament for treating neurodegenerative diseases, especially for ameliorating dyskinesia, comprising as an active ingredient selenoprotein P and/or a peptide fragment or a series of peptide fragments derived from said protein is provided. The novel medicament for treating neurodegenerative diseases, especially for ameliorating dyskinesia, according to the present invention is suitably applicable to diseases with decrease in motor function.
摘要翻译： 提供了用于治疗神经变性疾病，特别是用于改善运动障碍的新型药物，其包含作为活性成分的硒蛋白P和/或肽片段或一系列源自所述蛋白质的肽片段。 根据本发明的用于治疗神经变性疾病，特别是用于改善运动障碍的新型药物适用于运动功能降低的疾病。
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公开(公告)号：US20050143310A1
公开(公告)日：2005-06-30
申请号：US10477216
申请日：2002-05-10
申请人： Masaki Hirashima , Takeshi Naruse , Hiroaki Maeda , Chikateru Nozaki , Takeshi Goto , Katsuhiko Akiyama , Hidenao Fukushima
发明人： Masaki Hirashima , Takeshi Naruse , Hiroaki Maeda , Chikateru Nozaki , Takeshi Goto , Katsuhiko Akiyama , Hidenao Fukushima
CPC分类号： A61K38/1709
摘要： A medicament for treating neurodegenerative diseases, comprising as an active ingredient selenoprotein P and/or a peptide fragment or a series of peptide fragments derived from the C-terminal of selenoprotein P. An excellent medicament for treating neurodegenerative diseases, especially suitable for treating neurodegenerative diseases with ataxia as a principal symptom is provided.
摘要翻译： 一种用于治疗神经变性疾病的药物，其包含作为活性成分的硒蛋白P和/或肽片段或一系列衍生自硒蛋白P的C末端的肽片段。用于治疗神经变性疾病的优异药物，特别适用于治疗神经变性疾病 提供共济失调作为主要症状。
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公开(公告)号：US07598349B2
公开(公告)日：2009-10-06
申请号：US11185859
申请日：2005-07-21
IPC分类号： C07K16/00
CPC分类号： C07K14/4747 , A61K38/00
摘要： The present invention provides a peptide fragment or a series of peptide fragments having a cell death-inhibitory activity, having the amino acid sequence consisting of 103 amino acid residues at the C-terminal of selenoprotein P, or having said amino acid sequence with one or several amino acid residues therein being deleted, substituted or added, or having a partial sequence of either of the above amino acid sequences, a medicament for treatment comprising said peptide fragment or a series of peptide fragments, an antibody to said peptide fragment or a series of peptide fragments, and a method for screening a cell death-inhibitory activity using said peptide fragment or a series of peptide fragments. The preferable peptide fragment or a series of peptide fragments of the present invention has the amino acid sequences shown in SEQ ID NO: 1 and/or SEQ ID NO: 2 or has a partial sequence thereof.
摘要翻译： 本发明提供具有细胞死亡抑制活性的肽片段或一系列肽片段，其具有在硒蛋白P的C末端由103个氨基酸残基组成的氨基酸序列，或者具有所述氨基酸序列与一个或多个 其中缺少，取代或添加的几个氨基酸残基，或具有上述氨基酸序列之一的部分序列，包含所述肽片段或一系列肽片段的治疗药物，所述肽片段的抗体或一系列 的肽片段，以及使用所述肽片段或一系列肽片段筛选细胞死亡抑制活性的方法。 本发明优选的肽片段或一系列肽片段具有SEQ ID NO：1和/或SEQ ID NO：2所示的氨基酸序列或其部分序列。
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公开(公告)号：US20090075865A1
公开(公告)日：2009-03-19
申请号：US12081991
申请日：2008-04-24
CPC分类号： A61K38/1709
摘要： A novel medicament for treating neurodegenerative diseases, especially for ameliorating dyskinesia, comprising as an active ingredient selenoprotein P and/or a peptide fragment or a series of peptide fragments derived from said protein is provided. The novel medicament for treating neurodegenerative diseases, especially for ameliorating dyskinesia, according to the present invention is suitably applicable to diseases with decrease in motor function.
摘要翻译： 提供了用于治疗神经变性疾病，特别是用于改善运动障碍的新型药物，其包含作为活性成分的硒蛋白P和/或肽片段或一系列源自所述蛋白质的肽片段。 根据本发明的用于治疗神经变性疾病，特别是用于改善运动障碍的新型药物适用于运动功能降低的疾病。
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公开(公告)号：US07199097B1
公开(公告)日：2007-04-03
申请号：US09856199
申请日：1999-11-12
CPC分类号： C07K14/4747 , A61K38/00
摘要： The present invention provides a peptide fragment or a series of peptide fragments having a cell death-inhibitory activity, having the amino acid sequence consisting of 103 amino acid residues at the C-terminal of selenoprotein P, or having said amino acid sequence with one or several amino acid residues therein being deleted, substituted or added, or having a partial sequence of either of the above amino acid sequences, a medicament for treatment comprising said peptide fragment or a series of peptide fragments, an antibody to said peptide fragment or a series of peptide fragments, and a method for screening a cell death-inhibitory activity using said peptide fragment or a series of peptide fragments. The preferable peptide fragment or a series of peptide fragments of the present invention has the amino acid sequences shown in SEQ ID NO: 1 and/or SEQ ID NO: 2 or has a partial sequence thereof.
摘要翻译： 本发明提供具有细胞死亡抑制活性的肽片段或一系列肽片段，其具有在硒蛋白P的C末端由103个氨基酸残基组成的氨基酸序列，或者具有所述氨基酸序列与一个或多个 其中缺少，取代或添加的几个氨基酸残基，或具有上述氨基酸序列之一的部分序列，包含所述肽片段或一系列肽片段的治疗药物，所述肽片段的抗体或一系列 的肽片段，以及使用所述肽片段或一系列肽片段筛选细胞死亡抑制活性的方法。 本发明优选的肽片段或一系列肽片段具有SEQ ID NO：1和/或SEQ ID NO：2所示的氨基酸序列或其部分序列。
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10.发明申请Peptide having cytotoxicity inhibitory activity and method of screening these peptide having cytotoxicity inhibitory activity 失效具有细胞毒性抑制活性的肽和筛选具有细胞毒性抑制活性的肽的方法公开(公告)号：US20050143311A1
公开(公告)日：2005-06-30
申请号：US10477101
申请日：2002-05-10
申请人： Masaki Hirashima , Takeshi Naruse , Hiroaki Maeda , Chikateru Nozaki , Takeshi Goto , Katsuhiko Akiyama , Wataru Hattori
发明人： Masaki Hirashima , Takeshi Naruse , Hiroaki Maeda , Chikateru Nozaki , Takeshi Goto , Katsuhiko Akiyama , Wataru Hattori
CPC分类号： C07K14/4747 , C07K14/47 , G01N33/5014
摘要： A peptide fragment or a series of peptide fragments containing one or more selenocysteine that has a lowered toxicity than selenocystine and that exhibits a cytotoxicity-inhibitory activity. The peptide fragment or a series of peptide fragments according to the present invention has preferably the amino acid sequence from 260th to 362nd amino acid residues from the C-terminal of selenoprotein P, or said amino acid sequence with one or several amino acid residues therein being deleted, substituted or added, or a partial sequence of either of the above amino acid sequences, or an amino acid sequence comprising as a part any of the above amino acid sequences. A screening method for a peptide fragment having the cytotoxicity-inhibitory activity is also provided.
摘要翻译： 含有一种或多种硒代半胱氨酸的肽片段或一系列肽片段，其毒性低于硒代胱氨酸并且具有细胞毒性抑制活性。 根据本发明的肽片段或一系列肽片段优选具有来自硒蛋白P的C末端的第260至第362位氨基酸残基的氨基酸序列，或其中具有一个或几个氨基酸残基的所述氨基酸序列是 缺失，取代或添加，或上述氨基酸序列中的任一个的部分序列，或包含作为上述氨基酸序列之一的氨基酸序列。 还提供了具有细胞毒性抑制活性的肽片段的筛选方法。
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