公开(公告)号：US07456378B2

公开(公告)日：2008-11-25

申请号：US11805378

申请日：2007-05-22

申请人： Hiroshi Sasaki ,  Eiji Yokoi ,  Tatsuo Nakata

发明人： Hiroshi Sasaki ,  Eiji Yokoi ,  Tatsuo Nakata

IPC分类号： H01J40/14 ,  G02B7/04 ,  G02B21/00

CPC分类号： G02B21/16 ,  G02B21/0032 ,  G02B21/004

摘要： The invention provides a confocal microscope comprising a light source; a light scanning unit; an array device; a line-beam generating unit for imaging illumination light in the form of a straight line extending, on the array device, in a direction intersecting the scanning direction of the light scanning unit; an objective lens for imaging the illumination light reflected or transmitted at the array device on a specimen; a beamsplitter, between the array device and the light scanning unit, for splitting off from the illumination light detection light from the specimen; a two-dimensional image-acquisition unit for acquiring the split off detection light; and a control unit for controlling the light scanning unit and the array device, wherein the array device is disposed in an optically conjugate positional relationship with a focal plane of the objective lens, and the control unit performs control so as to synchronize the light scanning unit and the array device.

摘要翻译： 本发明提供一种包括光源的共焦显微镜; 光扫描单元; 阵列器件; 一个线束产生单元，用于在阵列装置上沿与光扫描单元的扫描方向相交的方向成直线的形式的照明光; 用于对在样品上的阵列装置反射或透射的照明光进行成像的物镜; 阵列器件和光扫描单元之间的分束器，用于从来自样本的照明光检测光分离; 用于获取分离检测光的二维图像获取单元; 以及控制单元，用于控制光扫描单元和阵列器件，其中阵列器件与物镜的焦平面以光学共轭位置关系设置，并且控制单元执行控制以使光扫描单元 和阵列设备。

公开(公告)号：US20070272843A1

公开(公告)日：2007-11-29

申请号：US11805378

申请日：2007-05-22

申请人： Hiroshi Sasaki ,  Eiji Yokoi ,  Tatsuo Nakata

发明人： Hiroshi Sasaki ,  Eiji Yokoi ,  Tatsuo Nakata

IPC分类号： H01J40/14 ,  H01J3/14

CPC分类号： G02B21/16 ,  G02B21/0032 ,  G02B21/004

摘要： The invention provides a confocal microscope comprising a light source; a light scanning unit; an array device; a line-beam generating unit for imaging illumination light in the form of a straight line extending, on the array device, in a direction intersecting the scanning direction of the light scanning unit; an objective lens for imaging the illumination light reflected or transmitted at the array device on a specimen; a beamsplitter, between the array device and the light scanning unit, for splitting off from the illumination light detection light from the specimen; a two-dimensional image-acquisition unit for acquiring the split off detection light; and a control unit for controlling the light scanning unit and the array device, wherein the array device is disposed in an optically conjugate positional relationship with a focal plane of the objective lens, and the control unit performs control so as to synchronize the light scanning unit and the array device.

摘要翻译： 本发明提供一种包括光源的共焦显微镜; 光扫描单元; 阵列器件; 一个线束产生单元，用于在阵列装置上沿与光扫描单元的扫描方向相交的方向成直线的形式的照明光; 用于对在样品上的阵列装置反射或透射的照明光进行成像的物镜; 阵列器件和光扫描单元之间的分束器，用于从来自样本的照明光检测光分离; 用于获取分离检测光的二维图像获取单元; 以及控制单元，用于控制光扫描单元和阵列器件，其中阵列器件与物镜的焦平面以光学共轭位置关系设置，并且控制单元执行控制以使光扫描单元 和阵列设备。

公开(公告)号：US07869132B2

公开(公告)日：2011-01-11

申请号：US12173111

申请日：2008-07-15

申请人： Masayoshi Saito ,  Eiji Yokoi ,  Kenichi Kusaka

发明人： Masayoshi Saito ,  Eiji Yokoi ,  Kenichi Kusaka

IPC分类号： G02B21/02

CPC分类号： G02B21/0032 ,  G02B9/60 ,  G02B15/163 ,  G02B21/02 ,  G02B21/33

摘要： An immersion microscope objective formed of thirteen or fewer lens elements includes, in order from the object side, first and second lens groups of positive refractive power, a third lens group, a fourth lens group having negative refractive power with its image-side surface being concave, and a fifth lens group having positive refractive power with its object-side surface being concave. The first lens group includes, in order from the object side, a lens component that consists of a lens element of positive refractive power (when computed as being in air) and a meniscus lens element having its concave surface on the object side. Various conditions are satisfied to ensure that images of fluorescence, obtained when the immersion microscope objective is used in a laser scanning microscope that employs multiphoton excitation to observe a specimen, are bright and of high resolution. Various laser scanning microscopes are also disclosed.

摘要翻译： 由十三个或更少的透镜元件形成的浸没显微镜物镜包括从物体侧起依次具有正屈光力的第一和第二透镜组，第三透镜​​组，具有负折射力的第四透镜组，其像侧表面为 具有正折射力的第五透镜组，其物侧表面是凹形的。 第一透镜组从物体的顺序依次包括由正屈光力的透镜元件（当计算为空气时）和在物体侧具有凹面的弯月透镜元件构成的透镜部件。 满足各种条件以确保当在使用多光子激发观察样品的激光扫描显微镜中使用浸没显微镜物镜时获得的荧光图像是明亮且高分辨率的。 还公开了各种激光扫描显微镜。

公开(公告)号：US20090073552A1

公开(公告)日：2009-03-19

申请号：US12200855

申请日：2008-08-28

申请人： Eiji Yokoi

发明人： Eiji Yokoi

IPC分类号： G02B21/18

CPC分类号： G02B21/0048 ,  G02B21/0076

摘要： A laser scanning microscope is disclosed that combines light from first and second scanning optical systems onto a common optical path using a beam combiner. A first plane-parallel transparent plate and a beam combiner are positioned on a support that may be moved relative to the common optical path. The first plane-parallel transparent plate has an optical thickness equal to the optical thickness of the beam combiner and is oriented with its surface normal lying substantially parallel with the surface normal of the beam combiner. An astigmatism-correcting optical element is provided on the common optical path, with the astigmatism-correcting optical element generating astigmatism that is equal in magnitude, but different in direction by 90 degrees, to the astigmatism generated by non-collimated light that is transmitted through the beam combiner. When the support is repositioned, spherical aberration in various light paths is maintained constant so that it may be easily corrected.

摘要翻译： 公开了一种激光扫描显微镜，其将来自第一和第二扫描光学系统的光组合到使用光束组合器的公共光路上。 第一平面平行透明板和光束组合器定位在可以相对于公共光路移动的支撑件上。 第一平面平行透明板具有等于光束组合器的光学厚度的光学厚度，并且其表面法线定向成基本上平行于光束组合器的表面法线。 在公共光路上设置散光校正光学元件，其中像散校正光学元件产生像差大小不同但方向不同90度的散光，与散射的非准直光产生的像散 光束组合器。 当支撑件重新定位时，各种光路中的球面像差保持恒定，从而可以容易地校正。

公开(公告)号：US08576482B2

公开(公告)日：2013-11-05

申请号：US12916240

申请日：2010-10-29

申请人： Masayoshi Saito ,  Eiji Yokoi ,  Kenichi Kusaka

发明人： Masayoshi Saito ,  Eiji Yokoi ,  Kenichi Kusaka

IPC分类号： G02B21/00 ,  G02B21/33

CPC分类号： G02B21/0032 ,  G02B9/60 ,  G02B15/163 ,  G02B21/02 ,  G02B21/33

摘要： An immersion microscope objective formed of thirteen or fewer lens elements includes, in order from the object side, first and second lens groups of positive refractive power, a third lens group, a fourth lens group having negative refractive power with its image-side surface being concave, and a fifth lens group having positive refractive power with its object-side surface being concave. The first lens group includes, in order from the object side, a lens component that consists of a lens element of positive refractive power (when computed as being in air) and a meniscus lens element having its concave surface on the object side. Various conditions are satisfied to ensure that images of fluorescence, obtained when the immersion microscope objective is used in a laser scanning microscope that employs multiphoton excitation to observe a specimen, are bright and of high resolution. Various laser scanning microscopes are also disclosed.

摘要翻译： 由十三个或更少的透镜元件形成的浸没显微镜物镜包括从物体侧起依次具有正屈光力的第一和第二透镜组，第三透镜​​组，具有负折射力的第四透镜组，其像侧表面为 具有正折射力的第五透镜组，其物侧表面是凹形的。 第一透镜组从物体的顺序依次包括由正屈光力的透镜元件（当计算为空气时）和在物体侧具有凹面的弯月透镜元件构成的透镜部件。 满足各种条件以确保当在使用多光子激发观察样品的激光扫描显微镜中使用浸没显微镜物镜时获得的荧光图像是明亮且高分辨率的。 还公开了各种激光扫描显微镜。

公开(公告)号：US07915575B2

公开(公告)日：2011-03-29

申请号：US12250680

申请日：2008-10-14

申请人： Eiji Yokoi

发明人： Eiji Yokoi

IPC分类号： H01J40/14 ,  H01L25/00

CPC分类号： G02B21/16 ,  G02B21/002 ,  G02B21/0032 ,  G02B21/0076

摘要： In a laser scanning microscope comprising an infrared pulse laser; an objective lens focusing an infrared light from the infrared pulse laser on a sample; a condenser lens disposed on an opposite side of the objective lens across the sample for collecting an observation light that is generated by a nonlinear optical effect and has a wavelength shorter than a wavelength of the infrared light; a visible light detector detecting the observation light collected by the condenser lens, an IR partial transmission filter having partially-modified transmission characteristics for the infrared light is disposed near a front focal position of the condenser lens, and an infrared light detector detecting, through the IR partial transmission filter, a transmitted light from the sample collected by the condenser lens, is provided.

摘要翻译： 在包括红外脉冲激光器的激光扫描显微镜中; 将来自红外脉冲激光器的红外光聚焦在样品上的物镜; 聚光透镜，其设置在所述物镜的与所述样品相反的一侧上，用于收集由非线性光学效应产生并具有比所述红外光的波长短的波长的观察光; 检测由聚光透镜收集的观察光的可见光检测器，在聚光透镜的前焦点附近设置红外光透射特性部分变化的IR部分透射滤光器，红外光检测器通过 IR部分透射滤光片，由聚光透镜收集的样品的透射光。

公开(公告)号：US08730582B2

公开(公告)日：2014-05-20

申请号：US13412040

申请日：2012-03-05

申请人： Eiji Yokoi

发明人： Eiji Yokoi

IPC分类号： G02B21/02 ,  G02F1/01

CPC分类号： G02B21/02 ,  G02B21/0032 ,  G02B21/0076 ,  G02B21/32 ,  G02B2207/114

摘要： A microscope apparatus includes a laser beam source for emitting a laser beam, an objective lens for irradiating a sample with the laser beam, a phase-modulating spatial light modulator placed between the laser beam source and the objective lens at a position optically conjugate with a pupil position of the objective lens, and a beam diameter variable unit placed between the laser beam source and the phase-modulating spatial light modulator for varying a beam diameter of the laser beam incident to the phase-modulating spatial light modulator.

摘要翻译： 显微镜装置包括用于发射激光束的激光束源，用于用激光束照射样品的物镜;相位调制空间光调制器，其设置在激光束源和物镜之间的光学共轭处的位置 物镜的光瞳位置以及放置在激光束源和相位调制用空间光调制器之间的光束直径可变单元，用于改变入射到相位调制空间光调制器的激光束的光束直径。

公开(公告)号：US08471218B2

公开(公告)日：2013-06-25

申请号：US12874451

申请日：2010-09-02

申请人： Eiji Yokoi

发明人： Eiji Yokoi

IPC分类号： G01N21/64

CPC分类号： G01J3/02 ,  G01J3/0227 ,  G01J3/0229 ,  G01J3/0235 ,  G01J3/10 ,  G01J3/4406

摘要： A detecting device includes a wavelength dispersion element for dispersing light into wavelengths and for emitting dispersed light, a photodetector for detecting the dispersed light, and a wavelength restriction element, which is arranged between the wavelength dispersion element and the photodetector and has an optical characteristic dependent on a wavelength, for restricting an incidence of light having a particular wavelength to the photodetector. Light that is part of the dispersed light and includes the light having has the particular wavelength is incident to the wavelength restriction element.

摘要翻译： 检测装置包括用于将光分散成波长并用于发射分散光的波长色散元件，用于检测分散光的光电检测器和布置在波长色散元件和光电检测器之间的波长限制元件，并具有光学特性依赖性 用于限制具有特定波长的光的入射到光电检测器的波长。 作为分散光的一部分并且包括具有特定波长的光的光入射到波长限制元件。

公开(公告)号：US08270088B2

公开(公告)日：2012-09-18

申请号：US12200855

申请日：2008-08-28

申请人： Eiji Yokoi

发明人： Eiji Yokoi

IPC分类号： G02B27/14

CPC分类号： G02B21/0048 ,  G02B21/0076

摘要： A laser scanning microscope is disclosed that combines light from first and second scanning optical systems onto a common optical path using a beam combiner. A first plane-parallel transparent plate and a beam combiner are positioned on a support that may be moved relative to the common optical path. The first plane-parallel transparent plate has an optical thickness equal to the optical thickness of the beam combiner and is oriented with its surface normal lying substantially parallel with the surface normal of the beam combiner. An astigmatism-correcting optical element is provided on the common optical path, with the astigmatism-correcting optical element generating astigmatism that is equal in magnitude, but different in direction by 90 degrees, to the astigmatism generated by non-collimated light that is transmitted through the beam combiner. When the support is repositioned, spherical aberration in various light paths is maintained constant so that it may be easily corrected.

摘要翻译： 公开了一种激光扫描显微镜，其将来自第一和第二扫描光学系统的光组合到使用光束组合器的公共光路上。 第一平面平行透明板和光束组合器定位在可以相对于公共光路移动的支撑件上。 第一平面平行透明板具有等于光束组合器的光学厚度的光学厚度，并且其表面法线定向成基本上平行于光束组合器的表面法线。 在公共光路上设置散光校正光学元件，其中像散校正光学元件产生像差大小不同但方向不同90度的散光，与散射的非准直光产生的像散 光束组合器。 当支撑件重新定位时，各种光路中的球面像差保持恒定，从而可以容易地校正。

公开(公告)号：US06459484B1

公开(公告)日：2002-10-01

申请号：US09692141

申请日：2000-10-20

申请人： Eiji Yokoi

发明人： Eiji Yokoi

IPC分类号： G01N2164

CPC分类号： G01N21/6456 ,  G01J3/02 ,  G01J3/021 ,  G01J3/0229 ,  G01J3/0248 ,  G01J3/14 ,  G01J3/36 ,  G01J3/4406 ,  G01N2021/6441 ,  G02B21/0076 ,  G02B21/008

摘要： A scanning optical apparatus includes a light source unit, an objective lens, a scanning device, an imaging optical system, a confocal aperture, a plurality of photodetectors, a spectrum decomposing element, and a light-deflecting microelement array. The light source unit is located at the position where illumination light from the light source unit is incident through the light-deflecting microelement array on the confocal aperture. Each of light-deflecting microelements constituting the light-deflecting microelement array has deflection angles for selectively deflecting light passing through the confocal aperture toward the plurality of photodetectors and a deflection angle for deflecting the light from the light source unit toward the confocal aperture so that each of the light-deflecting microelements is capable of selecting one of these deflection angles.

摘要翻译： 扫描光学装置包括光源单元，物镜，扫描装置，成像光学系统，共焦孔，多个光电检测器，光谱分解元件和光偏转微元件阵列。 光源单元位于通过共焦孔径上的光偏转微元件阵列入射来自光源单元的照明光的位置。 构成光偏转微元件阵列的每个光偏转微元件具有用于选择性地将通过共焦孔的光朝向多个光电检测器偏转的偏转角以及用于将来自光源单元的光偏转到共焦孔的偏转角， 的光偏转微元件能够选择这些偏转角之一。