公开(公告)号：US20050203707A1

公开(公告)日：2005-09-15

申请号：US10509728

申请日：2003-08-08

申请人： Hiroshi Tsutsui ,  Yasuhiro Mori ,  Hirosi Yamamoto ,  Shiro Nankai ,  Nobuyuki Shigetoh ,  Tatsurou Kawamura

发明人： Hiroshi Tsutsui ,  Yasuhiro Mori ,  Hirosi Yamamoto ,  Shiro Nankai ,  Nobuyuki Shigetoh ,  Tatsurou Kawamura

IPC分类号： A61B5/00 ,  G06F19/00

CPC分类号： G16H10/40 ,  A61B5/14532

摘要： The data measuring unit 11 measures a subject's blood and urine, and acquires numerical data (S501). The communication control unit 12 transmits the acquired numerical data to the data management apparatus 20 (S502). The overall control unit 14 receives the numerical data from the data measuring apparatus 10 (S503), and analyzes the content of the received numerical data (S504). The overall control unit 14 selects calibration curve data based on the above mentioned analysis result (S505), and calculates health care data (S506). Furthermore, the overall control unit 14 directs the communication control unit 13 to transmit the calculated health care data to the data measuring apparatus 10 (S507). The data measuring apparatus 10 receives the health care data from the data management apparatus 20 (S508).

摘要翻译： 数据测量单元11测量受试者的血液和尿液，并获取数字数据（S 501）。 通信控制单元12将获取的数字数据发送到数据管理装置20（步骤502）。 总体控制单元14从数据测量装置10接收数字数据（S 503），并分析接收数字数据的内容（S 504）。 总体控制单元14基于上述分析结果选择校准曲线数据（S 505），并计算保健数据（S 506）。 此外，总体控制单元14引导通信控制单元13将计算出的医疗保健数据发送到数据测量装置10（S 507）。 数据测量装置10从数据管理装置20接收保健数据（S 508）。

公开(公告)号：US07267750B2

公开(公告)日：2007-09-11

申请号：US10466561

申请日：2002-01-10

申请人： Motokazu Watanabe ,  Tomohiro Yamamoto ,  Miwa Hasegawa ,  Shin Ikeda ,  Shiro Nankai

发明人： Motokazu Watanabe ,  Tomohiro Yamamoto ,  Miwa Hasegawa ,  Shin Ikeda ,  Shiro Nankai

IPC分类号： G01N27/327

CPC分类号： C12Q1/005 ,  C12Q1/004

摘要： A biosensor comprising: an electrically insulating base plate; an electrode system comprising a working electrode and a counter electrode formed on the base plate; a cover member which is joined to the base plate to form a sample solution supply pathway for supplying a sample solution to the electrode system between the cover member and the base plate; and a reagent system comprising at least an oxidoreductase and an electron mediator disposed in the sample solution supply pathway, wherein the electron mediator is provided on the base plate and the oxidoreductase is provided on the cover member such that the electron mediator and the oxidoreductase are not in contact with each other.

摘要翻译： 一种生物传感器，包括：电绝缘基板; 电极系统，包括形成在所述基板上的工作电极和对电极; 盖构件，其连接到所述基板，以形成用于将样品溶液供应到所述盖构件和所述基板之间的所述电极系统的样品溶液供给路径; 以及试剂系统，其至少包含设置在样品溶液供给路径中的氧化还原酶和电子介体，其中，所述电子介体设置在所述底板上，所述氧化还原酶设置在所述覆盖部件上，使得所述电子介体和所述氧化还原酶不是 彼此接触。

公开(公告)号：US07235170B2

公开(公告)日：2007-06-26

申请号：US10344671

申请日：2002-05-13

申请人： Motokazu Watanabe ,  Takahiro Nakaminami ,  Shin Ikeda ,  Shiro Nankai

发明人： Motokazu Watanabe ,  Takahiro Nakaminami ,  Shin Ikeda ,  Shiro Nankai

IPC分类号： C12Q1/26

CPC分类号： C12Q1/006 ,  C12Q1/001 ,  C12Q1/54

摘要： A biosensor that is highly responsive and capable of rapid and highly sensitive quantification of a specific component contained in a sample is provided. The biosensor of this invention comprises: an electrically insulating base plate; an electrode system comprising a working electrode and a counter electrode disposed on the base plate; and a reagent system comprising an oxidoreductase which catalyzes the oxidation reaction of glucose, gluconolactonase and a buffer. The buffer is selected from the group consisting of phthalic acid and its salts, maleic acid and its salts, succinic acid and its salts, phospholic acid and its salts, acetic acid and its salts, boric acid and its salts, citric acid and its salts, glycine, tris(hydroxymethyl)aminomethane, piperazine-N,N′-bis(2-ethane sulfonic acid) and the like.

摘要翻译： 提供了一种生物传感器，其具有高响应性并且能够对包含在样品中的特定成分进行快速和高灵敏度的定量。 本发明的生物传感器包括：电绝缘基板; 电极系统，包括设置在所述基板上的工作电极和对置电极; 以及包含催化葡萄糖，葡糖酸内切酶和缓冲液的氧化反应的氧化还原酶的试剂体系。 缓冲剂选自邻苯二甲酸及其盐，马来酸及其盐，琥珀酸及其盐，磷酸及其盐，乙酸及其盐，硼酸及其盐，柠檬酸及其盐 ，甘氨酸，三（羟甲基）氨基甲烷，哌嗪-N，N'-双（2-乙磺酸）等。

公开(公告)号：US06656702B1

公开(公告)日：2003-12-02

申请号：US09345676

申请日：1999-06-30

申请人： Keiko Yugawa ,  Toshihiko Yoshioka ,  Shiro Nankai

发明人： Keiko Yugawa ,  Toshihiko Yoshioka ,  Shiro Nankai

IPC分类号： C12Q132

CPC分类号： C12Q1/005

摘要： A biosensor that enables rapid and simple quantitation of a substrate with high accuracy and demonstrates an excellent preservation characteristic by best retaining enzyme activity is disclosed. The biosensor comprises an electrically insulating base plate, an electrode system including at least a working electrode and a counter electrode formed on the base plate, and a reaction layer containing at least an enzyme and a sugar. The reaction layer may be formed on the electrode system. The enzyme may be selected from glucose oxidase, glucose dehydrogenase, and fructose dehydrogenase. The sugar is trehalose, sucrose, glycerol, mannitol, and ribose. If the enzyme selected is glucose dehydrogenase whose coenzyme is pyrrolo-quinoline quinone, the use of trehalose is preferable.

摘要翻译： 公开了一种生物传感器，其能够高精度地快速和简单地定量基质，并通过最佳的保留酶活性表现出优异的保存特性。 生物传感器包括电绝缘基板，至少包括形成在基板上的工作电极和对电极的电极系统和至少含有酶和糖的反应层。 反应层可以形成在电极系统上。 酶可以选自葡萄糖氧化酶，葡萄糖脱氢酶和果糖脱氢酶。 糖是海藻糖，蔗糖，甘油，甘露糖醇和核糖。 如果选择的酶是辅酶是吡咯并喹啉醌的葡萄糖脱氢酶，则优选使用海藻糖。

公开(公告)号：US06547954B2

公开(公告)日：2003-04-15

申请号：US09232320

申请日：1999-01-18

申请人： Shin Ikeda ,  Tomohiro Yamamoto ,  Toshihiko Yoshioka ,  Shiro Nankai ,  Shigeki Joko ,  Junko Iwata

发明人： Shin Ikeda ,  Tomohiro Yamamoto ,  Toshihiko Yoshioka ,  Shiro Nankai ,  Shigeki Joko ,  Junko Iwata

IPC分类号： G01N27327

CPC分类号： C12Q1/004 ,  Y10S435/817

摘要： A biosensor includes: an insulating substrate; an electrode system formed on the insulating substrate which has a working electrode and a counter electrode; and a reaction layer formed on the insulating substrate which contains an oxidoreductase and an electron acceptor. The electron acceptor is ferricinium ion derived from ferrocene electrolyte.

摘要翻译： 生物传感器包括：绝缘基板; 形成在具有工作电极和对电极的绝缘基板上的电极系统; 以及形成在包含氧化还原酶和电子受体的绝缘基板上的反应层。 电子受体是衍生自二茂铁电解质的三价铁离子。

公开(公告)号：US06436255B2

公开(公告)日：2002-08-20

申请号：US09764142

申请日：2001-01-19

申请人： Tomohiro Yamamoto ,  Motokazu Watanabe ,  Shin Ikeda ,  Shiro Nankai

发明人： Tomohiro Yamamoto ,  Motokazu Watanabe ,  Shin Ikeda ,  Shiro Nankai

IPC分类号： G01N27327

CPC分类号： C12Q1/005

摘要： The present invention provides a biosensor which enables prompt quantification by facilitating introduction of a sample solution even when the amount of the sample solution is small and improving dissolution of reagents. The biosensor comprises an insulating base plate; an electrode system including at least a measuring electrode and a counter electrode formed on the base plate; a cover member integrated into the base plate to form a sample solution supply pathway for supplying a sample solution to the electrode system between the cover member and the base plate; a reaction reagent system including at least an electron mediator and an oxidoreductase; and a carrier supporting at least the electron mediator or the oxidoreductase of the reaction reagent system, wherein the carrier is partially or wholly disposed outside the sample solution supply pathway.

摘要翻译： 本发明提供一种生物传感器，其即使在样品溶液量少且改善试剂溶解的情况下也能够通过促进样品溶液的引入而迅速进行定量。 生物传感器包括绝缘基板; 电极系统，其至少包括形成在所述基板上的测量电极和对电极; 集成到所述基板中以形成用于将样品溶液供应到所述盖构件和所述基板之间的所述电极系统的样品溶液供给路径的盖构件; 至少包括电子介体和氧化还原酶的反应试剂系统; 和至少支持反应试剂体系的电子介体或氧化还原酶的载体，其中载体被部分或全部置于样品溶液供给路径的外部。

公开(公告)号：US06340428B1

公开(公告)日：2002-01-22

申请号：US09424715

申请日：1999-11-29

申请人： Shin Ikeda ,  Toshihiko Yoshioka ,  Shiro Nankai

发明人： Shin Ikeda ,  Toshihiko Yoshioka ,  Shiro Nankai

IPC分类号： G01N2726

CPC分类号： C12Q1/004

摘要： A method for determining the concentration of a substrate in a sample solution using an electrode system comprising a working electrode, a counter electrode, and a reaction layer which contains at least an oxidoreductase and an electron mediator and is formed on the electrode system to electrochemically measure a reduced amount of the electron mediator resulting from enzyme reaction in the reaction layer, wherein a third electrode is formed as an interfering substance detecting electrode. A current flowing between the counter electrode and the third electrode is measured which is taken as a positive error. Subsequently, voltage application between the counter electrode and the third electrode is released and a voltage for oxidizing the reduced form electron mediator is applied between the working electrode and the counter electrode to measure a current flowing between the two electrodes.

摘要翻译： 一种使用包括工作电极，对电极和至少包含氧化还原酶和电子介体的反应层的电极系统来确定样品溶液中的衬底的浓度的方法，并且形成在电极系统上以电化学测量 在反应层中由酶反应产生的电子介体的量减少，其中形成第三电极作为干扰物质检测电极。 测量在对电极和第三电极之间流动的电流，其被认为是正误差。 随后，释放对电极和第三电极之间的电压施加，并且在工作电极和对电极之间施加用于氧化还原形式的电子介体的电压，以测量在两个电极之间流动的电流。

公开(公告)号：US06212417B1

公开(公告)日：2001-04-03

申请号：US09379128

申请日：1999-08-20

申请人： Shin Ikeda ,  Toshihiko Yoshioka ,  Shiro Nankai

发明人： Shin Ikeda ,  Toshihiko Yoshioka ,  Shiro Nankai

IPC分类号： A61B505

CPC分类号： G01N27/3272

摘要： A highly reliable biosensor is provided which is reduced in influences of any interfering substance or preexisting oxygen in a sample solution. The biosensor comprises an electrode system including a working electrode, a counter electrode and a third electrode also usable as an interfering substance detecting electrode, a reagent layer containing at least an oxidoreductase and an electron mediator, and an electrically insulating base plate for supporting the electrode system and the reagent layer, wherein the third electrode is arranged at an opposing position to that of at least one of the working electrode and the counter electrode and the reagent layer is arranged at a predetermined position apart from the third electrode.

摘要翻译： 提供了高度可靠的生物传感器，其减少了样品溶液中任何干扰物质或预先存在的氧气的影响。 生物传感器包括电极系统，其包括工作电极，对电极和也可用作干扰物质检测电极的第三电极，至少含有氧化还原酶和电子介体的试剂层，以及用于支撑电极的电绝缘基板 系统和试剂层，其中第三电极布置在与工作电极和对电极中的至少一个相对的位置，并且试剂层布置在与第三电极隔开的预定位置处。

公开(公告)号：US6104940A

公开(公告)日：2000-08-15

申请号：US326350

申请日：1999-06-04

申请人： Motokazu Watanabe ,  Shin Ikeda ,  Toshihiko Yoshioka ,  Shiro Nankai

发明人： Motokazu Watanabe ,  Shin Ikeda ,  Toshihiko Yoshioka ,  Shiro Nankai

IPC分类号： G01N27/30 ,  A61B5/00 ,  A61B5/145 ,  A61B5/1486 ,  A61B5/15 ,  A61B5/157 ,  A61B5/05

CPC分类号： A61B5/1486 ,  A61B5/6848

摘要： Disclosed is an electrode probe and a body fluid examination equipment enabling examination of a trace amount of body fluid. The electrode probe comprises an insulating needle member, a working electrode lead disposed in the needle member, a working electrode whose lead is protruding outside from the needle member, a counter electrode disposed on the surface of the needle member, a lead connected to the counter electrode, and a reagent layer disposed in contact with or in the vicinity of the working and counter electrodes of the electrode system. When a trace amount of body fluid leaking on the skin surface is brought into contact with the reagent layer, the reagent layer dissolves which results in electron transfer whereby information about the concentration of analyte in the body fluid can be obtained from the electrode system.

摘要翻译： 公开了能够检测微量体液的电极探针和体液检查装置。 电极探针包括绝缘针构件，设置在针构件中的工作电极引线，其引线从针构件向外突出的工作电极，设置在针构件表面上的对电极，连接到计数器的引线 电极和设置成与电极系统的工作电极和对电极接触或附近的试剂层。 当皮肤表面泄漏的微量体液与试剂层接触时，试剂层溶解，导致电子转移，从而可以从电极系统获得关于体液中分析物浓度的信息。

公开(公告)号：US6071392A

公开(公告)日：2000-06-06

申请号：US89350

申请日：1998-06-03

申请人： Tomohiro Yamamoto ,  Toshihiko Yoshioka ,  Shiro Nankai

发明人： Tomohiro Yamamoto ,  Toshihiko Yoshioka ,  Shiro Nankai

IPC分类号： C12Q1/00 ,  G01N27/26

CPC分类号： C12Q1/005 ,  Y10S435/817

摘要： A cholesterol sensor is disclosed which comprises an electrode system having a measuring electrode and a counter electrode formed on an electrically insulating base plate, an electrode coating layer for covering the electrode system and a reaction reagent layer formed on or in the vicinity of the electrode coating layer, wherein the reaction reagent layer comprises at least an enzyme for catalyzing cholesterol oxidation, an enzyme having a cholesterol ester hydrolyzing activity and a surfactant, the electrode coating layer comprises at least one selected from the group consisting of water-soluble cellulose derivatives and saccharides and is contained at such a concentration that imparts sufficient viscosity to a sample solution for enabling it to hinder invasion of said surfactant into said electrode system when said electrode coating layer is dissolved in said sample solution supplied to said sensor. This configuration best eliminates impairment of sensor response due to electrode degeneration caused by invading surfactant into the electrode system.

摘要翻译： 公开了一种胆固醇传感器，其包括具有测量电极和形成在电绝缘基板上的对电极的电极系统，用于覆盖电极系统的电极涂层和形成在电极涂层上或附近的反应试剂层 其中所述反应试剂层至少包含用于催化胆固醇氧化的酶，具有胆固醇酯水解活性的酶和表面活性剂，所述电极涂层包含选自水溶性纤维素衍生物和糖类中的至少一种 并且以使得当样品溶液溶解在提供给所述传感器的所述样品溶液中时能使其阻止所述表面活性剂侵入所述电极系统的浓度给予足够的粘度。 该配置最好地消除由于将表面活性剂侵入电极系统引起的电极变性的传感器响应的损害。