摘要:
A process is disclosed for continuously producing erythritol by cultivating erythritol-producing microorganisms under aerobic conditions. The process comprises the steps of: maintaining the concentration of dissolved oxygen in a culture broth in a fermentation tank at not less than 0.2 ppm; separating a part of the culture broth into a concentrated liquid in which the concentration of cells is increased and a clarified liquid by a cell separator; returning the concentrated liquid to the fermentation tank; controlling an amount of the clarified liquid to be extracted outside an erythritol-producing system and an amount of the culture broth and/or the concentrated liquid to be extracted outside the producing system such that the concentration of the cells in the culture broth in the fermentation tank may be kept in a range from 40 to 200 g/l when calculated as a weight of dried cells; and recovering erythritol from the clarified liquid.The clarified liquid containing erythritol may be separated from the culture broth by a cell separator arranged inside the fermentation tank, and the extracting amount of the erythritol-containing clarified liquid separated by the cell separator and an amount of the culture broth to be extracted may be controlled such that the concentration of the cells in the culture broth may be kept at 40 to 200 g/l when calculated as a weight of dried cells.
摘要:
Biologically pure culture of Aureobasidium sp. SN-124A strain (FERM BP-1429) and artificial mutants thereof according to the present invention show the properties of forming and accumulating erythritol in a culture solution, when aerobically cultured on a liquid culture medium containing an assimilable carbon source and an assimilable nitrogen source, and are useful for the preparation of erythritol by fermentation of sugars. A method for preparing erythritol by fermentation of sugars according to the present invention comprises inoculating microorganism selected from the group consisting of Aureobasidium sp. SN-124A strain (FERM BP-1429) and mutants thereof on a liquid culture medium of pH 4 to 9 containing an assimilable carbon source and an assimilable nitrogen source, and aerobically culturing them at a temperature of 30.degree. to 38.degree. C. to form and accumulate erythritol in said culture medium for collection.
摘要:
A buckle includes a male member including a base part and a pair of flexible arms extending from the base part, and a female member including an insertion hole formed at one end portion thereof. The pair of flexible arms is inserted in the insertion hole. The base part contacts the one end portion when the male member engages the female member. An outer face portion of each of the pair of flexible arms has a shape curving outwardly at a middle portion thereof relative to an insertion direction of the pair of flexible arms, and the pair of flexible arms is provisionally fastened to the female member when the pair of flexible arm is inserted in the insertion hole.
摘要:
A buckle includes a male member having a pair of flexible arms mutually facing each other and disposed extending from a base part, and a female member to connect to the male member by coupling with the pair of arms. The male member has a flexible bridge connecting the pair of arms. The bridge is connected to an opposing face of each of the pair of arms facing the other arm. At the connecting portion where each of the pair of arms and the bridge are connected, the bridge is placed projecting toward the other arm from the opposing face on the arm following a tangential direction to a circle being centered on the base end of the arm and having a distance from the base end to the connecting portion as a radius.
摘要:
A cord lock includes a male tool and a female tool that receives a part of the male tool by pressing the male tool against a biasing force of a biasing device, and so as to match cord eyelets formed in sides of the male tool and the female tool each other at a predetermined pressing position of the male tool. An engaging part that is engaged with an engaged part of the female tool by the biasing force of the biasing device to prevent the male tool from slipping out of a position before the pressing operation, is formed on a side of the male tool intersecting a direction in which the eyelet passes through, and at a position closer to a pressing backward side than a hole edge located on a pressing forward side of the eyelet.