公开(公告)号：US4939091A

公开(公告)日：1990-07-03

申请号：US88858

申请日：1987-08-24

申请人： Takashi Sasaki ,  Takafumi Kasumi ,  Naoya Kubo ,  Keiji Kainuma ,  Katsuo Wako ,  Hiroaki Ishizuka ,  Gaku Kawaguchi ,  Tsunero Oda

发明人： Takashi Sasaki ,  Takafumi Kasumi ,  Naoya Kubo ,  Keiji Kainuma ,  Katsuo Wako ,  Hiroaki Ishizuka ,  Gaku Kawaguchi ,  Tsunero Oda

IPC分类号： C12N1/02 ,  C12P7/18

CPC分类号： C12R1/645 ,  C12P7/18 ,  Y10S435/911

摘要： Biologically pure culture of Aureobasidium sp. SN-124A strain (FERM BP-1429) and artificial mutants thereof according to the present invention show the properties of forming and accumulating erythritol in a culture solution, when aerobically cultured on a liquid culture medium containing an assimilable carbon source and an assimilable nitrogen source, and are useful for the preparation of erythritol by fermentation of sugars. A method for preparing erythritol by fermentation of sugars according to the present invention comprises inoculating microorganism selected from the group consisting of Aureobasidium sp. SN-124A strain (FERM BP-1429) and mutants thereof on a liquid culture medium of pH 4 to 9 containing an assimilable carbon source and an assimilable nitrogen source, and aerobically culturing them at a temperature of 30.degree. to 38.degree. C. to form and accumulate erythritol in said culture medium for collection.

摘要翻译： 生物纯培养的Aureobasidium sp。 根据本发明的SN-124A菌株（FERM BP-1429）及其人造突变体，当在含有可同化的碳源和可同化的氮源的液体培养基上进行有氧培养时，显示培养液中形成和积累赤藓糖醇的性质 ，可用于通过发酵糖制备赤藓糖醇。 通过根据本发明的糖的发酵制备赤藓糖醇的方法包括接种微生物，其选自由Aureobasidium sp。 SN-124A菌株（FERM BP-1429）及其在pH4〜9的液体培养基上的突变体，其含有可同化的碳源和可吸收的氮源，并在30℃至38℃的温度下进行有氧培养， 在所述培养基中形成并积累赤藓糖醇用于收集。

公开(公告)号：US5036011A

公开(公告)日：1991-07-30

申请号：US504938

申请日：1990-04-05

申请人： Takashi Sasaki ,  Takafumi Kasumi ,  Naoya Kubo ,  Keiji Kainuma ,  Katsuo Wako ,  Hiroaki Ishizuka ,  Gaku Kawaguchi ,  Tsunero Oda

发明人： Takashi Sasaki ,  Takafumi Kasumi ,  Naoya Kubo ,  Keiji Kainuma ,  Katsuo Wako ,  Hiroaki Ishizuka ,  Gaku Kawaguchi ,  Tsunero Oda

IPC分类号： C12N1/02 ,  C12P7/18

CPC分类号： C12R1/645 ,  C12P7/18 ,  Y10S435/911

摘要： Biologically pure culture of Aureobasidium sp. SN-124A strain (FERM BP-1429) and artificial mutants thereof according to the present invention show the properties of forming and accumulating erythritol in a culture solution, when aerobically cultured on a liquid culture medium containing an assimilable carbon source and an assimilable nitrogen source, and are useful for the preparation of erythritol by fermentation of sugars. A method for preparing erythritol by fermentation of sugars according to the present invention comprises inoculating microorganism selected from the group consisting of Aureobasidium sp. SN-124A strain (FERM BP-1429) and mutants thereof on a liquid culture medium of pH 4 to 9 containing an assimilable carbon source and an assimilable nitrogen source, and aerobically culturing them at a temperature of 30.degree. to 38.degree. C. to form and accumulate erythritol in said culture medium for collection.

公开(公告)号：US4908311A

公开(公告)日：1990-03-13

申请号：US236555

申请日：1988-08-25

申请人： Takashi Sasaki ,  Hajime Taniguchi ,  Keiji Kainuma

发明人： Takashi Sasaki ,  Hajime Taniguchi ,  Keiji Kainuma

IPC分类号： C12P19/14 ,  C08B37/00 ,  C12N9/42 ,  C12P19/04 ,  C12R1/01

CPC分类号： C12P19/04 ,  C12N9/2445 ,  C12Y302/01021

摘要： A process for enzymatic preparation of cellooligosaccharide from a cellulose-base substance by the action of cellulase produced by an microorganism belonging to the Genus Cellvibrio, wherein an ultrafiltration reactor is used in combination, so that production inhibition can be removed and cellooligosaccharide can be formed and accumulated. The present process permits preparation of cellobiose in high yield and is very advantageous from an industrial standpoint.

摘要翻译： 通过由属于细菌属属微生物的微生物的纤维素酶的作用从纤维素基物质中酶制备纤维寡糖的方法，其中组合使用超滤反应器，从而可以除去生产抑制并形成低聚果糖; 积累。 本方法允许以高产率制备纤维二糖，并且从工业观点来看是非常有利的。

公开(公告)号：US4668626A

公开(公告)日：1987-05-26

申请号：US697774

申请日：1985-02-04

申请人： Shoichi Kobayashi ,  Keiji Kainuma

发明人： Shoichi Kobayashi ,  Keiji Kainuma

IPC分类号： C08B37/16 ,  C12P19/04 ,  C12P19/16 ,  C12P19/22 ,  C12P19/18 ,  C12P19/20

CPC分类号： C08B37/0012 ,  C12P19/04 ,  C12P19/16 ,  Y10T436/145555 ,  Y10T436/146666 ,  Y10T436/147777

摘要： The invention provides an efficient enzymatic method for the preparation of branched cyclodextrins such as glucosyl and maltosyl cyclodextrins. The inventive method comprises the enzymatic reaction of a branch-splitting enzyme and .beta.-amylase simultaneously with a mixture of a cyclodextrin and starch. Alternatively, a branched cyclodextrin is obtained from a mixture of a cyclodextrin and maltose in an enzymatic reaction with pullulanase, optionally, with admixture of an alcohol such as ethyl and propyl alcohols or a glycol such as ethyleneglycol and propyleneglycol to the reaction mixture. A glucosyl cyclodextrin can be obtained by the steps of first subjecting a mixture of a cyclodextrin and maltose to an enzymatic reaction with pullulanase and then subjecting the reaction product to a second enzymatic reaction in the presence of an enzyme mixture composed of takaamylase and glucoamylase and yeast.

摘要翻译： 本发明提供了制备支链环糊精如葡萄糖基和麦芽糖基环糊精的有效酶法。 本发明的方法包括分支酶和β-淀粉酶与环糊精和淀粉的混合物的酶反应。 或者，在与支链淀粉酶的酶反应中，可以从环糊精和麦芽糖的混合物中获得支化环糊精，任选地，与乙醇和丙醇之类的醇或二醇如乙二醇和丙二醇混合。 可以通过以下步骤获得葡糖基环糊精：首先使环糊精和麦芽糖的混合物与支链淀粉酶进行酶反应，然后在由塔卡淀粉酶和葡糖淀粉酶和酵母组成的酶混合物存在下对第二酶反应进行第二次酶反应 。

公开(公告)号：US4556057A

公开(公告)日：1985-12-03

申请号：US474302

申请日：1983-03-11

申请人： Teruo Hiruma ,  Atsushi Honma ,  Tooru Hirano ,  Yutaka Tsuchiya ,  Yoshihiro Hayata ,  Katsuo Aizawa ,  Harubumi Kato ,  Keiji Kainuma

发明人： Teruo Hiruma ,  Atsushi Honma ,  Tooru Hirano ,  Yutaka Tsuchiya ,  Yoshihiro Hayata ,  Katsuo Aizawa ,  Harubumi Kato ,  Keiji Kainuma

IPC分类号： A61B1/04 ,  A61B5/00 ,  A61B18/20 ,  A61N5/06 ,  G01J3/12 ,  G01N21/64 ,  A61B6/08

CPC分类号： A61B1/043 ,  A61B5/0071 ,  A61B5/0075 ,  A61B5/0084 ,  A61N5/0601 ,  A61N5/062 ,  G01N21/6456 ,  G01N2021/6417

摘要： Cancer diagnosis device contains an endoscope comprising a light pipe used to transmit light beams from respective light sources and an image guide used to observe the flesh of the organism during both diagnosis and treatment. The tip of the endoscope faces toward the focuses where a photosensitive material having an affinity to the focuses of cancer has been absorbed so that a treatment for cancer can be performed by exposing the organism affected by cancer to the laser beam. The attainable the laser beam within a focus is thus increased by the use of the laser beam pulse from a first laser beam pulse source. In addition to the above, the cancer diagnosis device contains a second laser beam pulse source used to transmit a light beam to the focuses through the light pipe for making a diagnosis, a selector used to selectively pick up the light beam from the first laser beam pulse source or the second laser beam pulse source, a spectroscope used to obtain the spectral response from emission of light led through the image guide used for observation from the flesh of the organism where fluorescence occurs, an imaging device used to pick up the spectral response, a graphic display used to graphically display the processed spectra picked up by the imaging device, and a controller used to control emission of light from the second laser beam pulse source and also to make the operation of the imaging device synchronize with the second laser beam pulse source.

摘要翻译： 癌症诊断装置包括内窥镜，其包括用于透射来自各个光源的光束的光管和用于在诊断和治疗期间观察生物体的肉体的图像引导件。 内窥镜的尖端朝向焦点，其中已经吸收了对癌症焦点具有亲和力的感光材料，从而可以通过将受癌症影响的生物体暴露于激光束来进行癌症治疗。 因此，可以通过使用来自第一激光束脉冲源的激光束脉冲来增加聚焦内的可达到的激光束。 除了上述之外，癌症诊断装置包含第二激光束脉冲源，用于通过光管将光束传输到焦点进行诊断，选择器用于选择性地拾取来自第一激光束的光束 脉冲源或第二激光束脉冲源，用于获得从发射的光的光谱响应的光谱仪，其通过用于从发生荧光的生物体的肉体观察的图像引导件，用于拾取光谱响应的成像装置 ，用于图形显示由成像装置拾取的处理光谱的图形显示器，以及用于控制来自第二激光束脉冲源的光的发射的控制器，并且还使成像装置的操作与第二激光束同步 脉冲源。

公开(公告)号：US4591560A

公开(公告)日：1986-05-27

申请号：US566499

申请日：1983-12-29

申请人： Keiji Kainuma ,  Shoichi Kobayashi

发明人： Keiji Kainuma ,  Shoichi Kobayashi

IPC分类号： C12N9/26 ,  C12N9/34 ,  C12P19/20 ,  C12R1/645 ,  C12N9/30 ,  C12P19/14

CPC分类号： C12N9/2411 ,  C12N9/242 ,  C12N9/2428 ,  C12P19/20 ,  Y10S435/911

摘要： A process for the saccharification of starch, which comprises saccharifying a raw and/or gelatinized starch by the use of an amylase produced by a fungus belonging to genus Chalara to produce glucose.According to the process of the present invention, the starch is directly saccharified, and glucose can be obtained efficiently.

摘要翻译： 淀粉糖化方法，其包括通过使用由属于Chalara属的真菌产生的淀粉酶糖化原始和/或糊化的淀粉以产生葡萄糖。 根据本发明的方法，淀粉直接糖化，可有效地获得葡萄糖。