公开(公告)号：US08652835B2

公开(公告)日：2014-02-18

申请号：US12525595

申请日：2008-01-31

申请人： Holger Link ,  Alexander Papra ,  Michael Blumentritt ,  Vinh Duong

发明人： Holger Link ,  Alexander Papra ,  Michael Blumentritt ,  Vinh Duong

IPC分类号： B01D15/08 ,  B01D63/00 ,  C02F1/44 ,  C12M1/00 ,  C12M3/00 ,  C12M1/36 ,  C12M1/38 ,  C12N5/00 ,  C12N5/02

CPC分类号： C12M45/02

摘要： A device for mechanically decollating cells from a cell composite, particularly a shear rotor, a cell isolation unit including such a shear rotor and a method for decollating cells from a cell composite. The aforementioned device includes a rotor having a rotor wall, which is concentrically arranged in a receptacle. A rotor seat is connected to a motor and to which the rotor can be fixed in a detachable manner. A rotational movement of the motor can be transmitted to the rotor by means of the rotor seat. The rotor tapers longitudinally towards the bottom of the receptacle so that different circumferential speeds of the rotor can be transmitted to a liquid sample in the receptacle via the rotor wall.

摘要翻译： 用于从细胞复合材料，特别是剪切转子，包括这种剪切转子的细胞隔离单元以及从细胞复合材料中分离细胞的方法来机械地去除细胞的装置。 上述装置包括具有同心地布置在容器中的转子壁的转子。 转子座连接到电动机上，转子可以以可拆卸的方式固定到转子座上。 电动机的旋转运动可以通过转子座传递到转子。 转子纵向朝向容器的底部逐渐变细，使得转子的不同圆周速度可以经由转子壁传递到容器中的液体样品。

公开(公告)号：US20100087003A1

公开(公告)日：2010-04-08

申请号：US12525595

申请日：2008-01-31

申请人： Holger Link ,  Alexander Papra ,  Michael Blumentritt ,  Vinh Duong

发明人： Holger Link ,  Alexander Papra ,  Michael Blumentritt ,  Vinh Duong

IPC分类号： C12N5/00 ,  C12M1/00 ,  C12M1/38

CPC分类号： C12M45/02

摘要： A device for mechanically decollating cells from a cell composite, particularly a shear rotor, a cell isolation unit including such a shear rotor and a method for decollating cells from a cell composite. The aforementioned device includes a rotor having a rotor wall, which is concentrically arranged in a receptacle. A rotor seat is connected to a motor and to which the rotor can be fixed in a detachable manner. A rotational movement of the motor can be transmitted to the rotor by means of the rotor seat. The rotor tapers longitudinally towards the bottom of the receptacle so that different circumferential speeds of the rotor can be transmitted to a liquid sample in the receptacle via the rotor wall.

摘要翻译： 用于从细胞复合材料，特别是剪切转子，包括这种剪切转子的细胞隔离单元以及从细胞复合材料中分离细胞的方法来机械地去除细胞的装置。 上述装置包括具有同心地布置在容器中的转子壁的转子。 转子座连接到电动机上，转子可以以可拆卸的方式固定到转子座上。 电动机的旋转运动可以通过转子座传递到转子。 转子纵向朝向容器的底部逐渐变细，使得转子的不同圆周速度可以经由转子壁传递到容器中的液体样品。

公开(公告)号：US20100150203A1

公开(公告)日：2010-06-17

申请号：US12534619

申请日：2009-08-03

申请人： Lutz TIMMANN ,  Vinh Duong ,  Stefan Roth ,  Thomas Uschkureit ,  Jürgen Koeppel ,  Thomas Buck ,  Michael Wild

发明人： Lutz TIMMANN ,  Vinh Duong ,  Stefan Roth ,  Thomas Uschkureit ,  Jürgen Koeppel ,  Thomas Buck ,  Michael Wild

IPC分类号： G01N25/00

CPC分类号： G01N25/00 ,  B01L7/52 ,  B01L2200/147 ,  B01L2200/148 ,  B01L2300/0627 ,  B01L2300/1822 ,  B01L2300/1827

摘要： The invention relates to a tempering apparatus for the execution of a tempering program for at least one sample, in particular PCR-sample, comprising: at least one tempering block, which is configured for the reception of at least one sample, at least one tempering device, which is arranged for tempering said at least one tempering block, at least one temperature measurement device, which is assigned to said tempering device, at least one control loop for the regulation of a temperature, to which at least one tempering device and at least one temperature measurement device, which is assigned to said at least one tempering device, are assigned to, at least one control device, which is configured for the control of the tempering of the at least one tempering block, wherein the tempering apparatus comprises at least two temperature measurement devices, which are assigned to at least one control loop, and that to the tempering apparatus a testing device for performing a test method is assigned to, wherein said testing device comprises a signal-connection to at least one of said at least two temperature measurement devices, such that by means of said signal-connection at least one testing quantity of the tempering apparatus is detectable, which characterizes the operational status of the tempering apparatus. The invention further relates to a method for testing of a tempering apparatus, wherein the method comprises the following steps: starting of the method; operation of at least said first tempering device for the duration of at least one first period from at least one first time; detection of at least one measurement temperature from said at least one first temperature measurement device, which is assigned to said first tempering device, at least at a second time; determining at least one first testing quantity of the tempering apparatus by using said at least one measurement temperature; and comparison of said first testing quantity with one reference quantity.

公开(公告)号：US07060488B2

公开(公告)日：2006-06-13

申请号：US10352526

申请日：2003-01-28

申请人： Vinh Duong ,  Andreas Graff ,  Cordula Kroll ,  Werner Lurz ,  Melanie Persson ,  Andreas Schirr ,  Wilhelm Pluster ,  Lutz Timmann

发明人： Vinh Duong ,  Andreas Graff ,  Cordula Kroll ,  Werner Lurz ,  Melanie Persson ,  Andreas Schirr ,  Wilhelm Pluster ,  Lutz Timmann

IPC分类号： C12M1/34

CPC分类号： B01L3/502715 ,  B01L3/502707 ,  B01L7/52 ,  B01L2200/027 ,  B01L2200/028 ,  B01L2300/0654 ,  B01L2300/0816 ,  B01L2300/087 ,  B01L2300/0874 ,  B01L2400/0487

摘要： A configuration of mini-volume reaction receptacles (1, 16, 41) of which the receptacle housings (2, 17) each enclose an elongated chamber (3, 18, 42) of which the ends are connected to apertures (6, 7, 20, 22) formed in the receptacle housing. The receptacle housings have identical base surfaces and have a small height relative to the base surface, and are stacked on one another while their base surfaces are mutually aligned. At least one aperture of a receptacle housing communicates with at least one aperture of a vertically adjacent receptacle housing, as seen in the direction of stacking. The receptacles (1, 16, 41) are mechanically interlocked in a direction transverse to the direction of stacking and can be plugged one into another. Each receptacle housing defines at least one aperture (6, 7, 22) at its top side that is accessible to a pipette.

摘要翻译： 微型体积反应容器（1,16,31）的构造，其中插座壳体（2,17）各自包围细长室（3,18,42），其中端部连接到孔（6,7） 20,22）形成在插座壳体中。 插座壳体具有相同的基面并且相对于基面具有小的高度，并且彼此堆叠在一起，同时它们的基面相互对准。 插座壳体的至少一个孔与垂直相邻的插座壳体的至少一个孔连通，如沿堆叠方向所见。 容器（1,16,41）在横向于堆叠方向的方向上机械地互锁，并且可以将其插入到另一个中。 每个插座壳体在其顶侧限定至少一个可移动管可接近的孔（6,7,22）。

公开(公告)号：US08322918B2

公开(公告)日：2012-12-04

申请号：US12534619

申请日：2009-08-03

申请人： Lutz Timmann ,  Vinh Duong ,  Stefan Roth ,  Thomas Uschkureit ,  Jurgen Koeppel ,  Thomas Buck ,  Michael Wild

发明人： Lutz Timmann ,  Vinh Duong ,  Stefan Roth ,  Thomas Uschkureit ,  Jurgen Koeppel ,  Thomas Buck ,  Michael Wild

IPC分类号： G01N25/00

CPC分类号： G01N25/00 ,  B01L7/52 ,  B01L2200/147 ,  B01L2200/148 ,  B01L2300/0627 ,  B01L2300/1822 ,  B01L2300/1827

摘要： The invention relates to a method for testing of a tempering apparatus, wherein the method comprises the following steps: starting of the method; operation of at least said first tempering device for the duration of at least one first period from at least one first time; detection of at least one measurement temperature from said at least one first temperature measurement device, which is assigned to said first tempering device, at least at a second time; determining at least one first testing quantity of the tempering apparatus by using said at least one measurement temperature; and comparison of said first testing quantity with one reference quantity.

摘要翻译： 本发明涉及回火设备的测试方法，其中该方法包括以下步骤：启动该方法; 至少所述第一回火装置至少从一个第一时间起至少一个第一周期的持续时间; 至少在第二时间从所述至少一个第一温度测量装置检测分配给所述第一回火装置的至少一个测量温度; 通过使用所述至少一个测量温度来确定所述回火设备的至少一个第一测试量; 以及所述第一测试量与一个参考量的比较。