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1.发明授权Thermostable type-A DNA polymerase mutants with increased polymerization rate and resistance to inhibitors 有权具有增加的聚合速率和对抑制剂的抗性的热稳定型A-DNA聚合酶突变体公开(公告)号：US09523085B2
公开(公告)日：2016-12-20
申请号：US12848765
申请日：2010-08-02
申请人： Holly Hogrefe , Michelle Cayouette , Jeffrey Fox , Connie J. Hansen , Jennifer Lapira , Bahram Arezi , Nancy McKinney
发明人： Holly Hogrefe , Michelle Cayouette , Jeffrey Fox , Connie J. Hansen , Jennifer Lapira , Bahram Arezi , Nancy McKinney
CPC分类号： C12N9/1252
摘要： The present invention provides mutants of DNA polymerases having an increased rate of incorporation of nucleotides into nucleic acids undergoing polymerization and having an enhanced resistance to inhibitors of DNA polymerase activity. The mutant polymerases are well suited for fast PCR applications, for PCR amplification of targets in samples that contain inhibitors of wild-type polymerases, and for fast PCR amplification of samples containing DNA polymerase inhibitors. In exemplary embodiments, the mutants are mutants of Taq DNA polymerase.
摘要翻译： 本发明提供了DNA聚合酶的突变体，其具有增加的核苷酸掺入到经历聚合的核酸中并且具有增强的抗DNA聚合酶活性抑制剂的能力的突变体。 突变型聚合酶非常适用于快速PCR应用，用于PCR扩增含有野生型聚合酶抑制剂的样品中的靶标，以及用于快速PCR扩增含有DNA聚合酶抑制剂的样品。 在示例性实施方案中，突变体是Taq DNA聚合酶的突变体。
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2.发明申请THERMOSTABLE TYPE-A DNA POLYMERASE MUTANTS WITH INCREASED POLYMERIZATION RATE AND RESISTANCE TO INHIBITORS 有权具有增加的聚合速率和耐药性的可热型TYPE-A DNA聚合酶突变体公开(公告)号：US20110027833A1
公开(公告)日：2011-02-03
申请号：US12848765
申请日：2010-08-02
申请人： Holly Hogrefe , Michelle Cayouette , Jeffrey Fox , Connie J. Hansen , Jennifer Lapira , Bahram Arezi , Nancy McKinney
发明人： Holly Hogrefe , Michelle Cayouette , Jeffrey Fox , Connie J. Hansen , Jennifer Lapira , Bahram Arezi , Nancy McKinney
CPC分类号： C12N9/1252
摘要： The present invention provides mutants of DNA polymerases having an increased rate of incorporation of nucleotides into nucleic acids undergoing polymerization and having an enhanced resistance to inhibitors of DNA polymerase activity. The mutant polymerases are well suited for fast PCR applications, for PCR amplification of targets in samples that contain inhibitors of wild-type polymerases, and for fast PCR amplification of samples containing DNA polymerase inhibitors. In exemplary embodiments, the mutants are mutants of Taq DNA polymerase.
摘要翻译： 本发明提供了DNA聚合酶的突变体，其具有增加的核苷酸掺入到经历聚合的核酸中并且具有增强的抗DNA聚合酶活性抑制剂的能力的突变体。 突变型聚合酶非常适用于快速PCR应用，用于PCR扩增含有野生型聚合酶抑制剂的样品中的靶标，以及用于快速PCR扩增含有DNA聚合酶抑制剂的样品。 在示例性实施方案中，突变体是Taq DNA聚合酶的突变体。
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公开(公告)号：US20050123940A1
公开(公告)日：2005-06-09
申请号：US10853973
申请日：2004-05-26
申请人： Joseph Sorge , Holly Hogrefe , Connie Hansen , Bahram Arezi , Rebecca Mullinax
发明人： Joseph Sorge , Holly Hogrefe , Connie Hansen , Bahram Arezi , Rebecca Mullinax
CPC分类号： B82Y5/00 , B82Y10/00 , C12N9/1252 , C12N9/1276 , C12N15/1096
摘要： The present invention relates to composition, kits and methods comprising a mutant DNA polymerase exhibiting increased reverse transcriptase activity. The invention also relates to methods of generating modified cDNA.
摘要翻译： 本发明涉及包含显示增加的逆转录酶活性的突变型DNA聚合酶的组合物，试剂盒和方法。 本发明还涉及产生修饰的cDNA的方法。
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公开(公告)号：US09783791B2
公开(公告)日：2017-10-10
申请号：US11502819
申请日：2006-08-10
申请人： Holly Hogrefe , Bahram Arezi , Weimei Xing
发明人： Holly Hogrefe , Bahram Arezi , Weimei Xing
CPC分类号： C12N9/1276 , C12N15/1096 , C12Q1/686 , C12Y207/07049 , C40B40/08 , C40B50/06
摘要： The invention relates to the generation and characterization of stable MMLV reverse transcriptase mutants. The invention also discloses methods of using stable MMLV reverse transcriptase mutants.
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公开(公告)号：US20080227661A1
公开(公告)日：2008-09-18
申请号：US11502819
申请日：2006-08-10
申请人： Holly Hogrefe , Bahram Arezi , Weimei Xing
发明人： Holly Hogrefe , Bahram Arezi , Weimei Xing
CPC分类号： C12N9/1276 , C12N15/1096 , C12Q1/686 , C12Y207/07049 , C40B40/08 , C40B50/06
摘要： The invention relates to the generation and characterization of stable MMLV reverse transcriptase mutants. The invention also discloses methods of using stable MMLV reverse transcriptase mutants.
摘要翻译： 本发明涉及稳定的MMLV逆转录酶突变体的产生和表征。 本发明还公开了使用稳定的MMLV逆转录酶突变体的方法。
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公开(公告)号：US20050118609A1
公开(公告)日：2005-06-02
申请号：US10861928
申请日：2004-06-04
申请人： Joseph Sorge , Bahram Arezi , Holly Hogrefe
发明人： Joseph Sorge , Bahram Arezi , Holly Hogrefe
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6827 , C12Q1/6818 , C12Q1/6858 , C12Q2535/125 , C12Q2525/161 , C12Q2565/101
摘要： The present invention relates to a method for identifying a nucleotide at a predetermined location on a target polynucleotide. The method involves single nucleotide extension reaction comprising an oligonucleotide primer comprising a first sequence and a second sequence or a tag. The method may further comprises a probe which hybridizes to the second sequence or an anti-tag molecule which interacts with the tag, where the hybridization or interaction causes a detectable signal transfer which is indicative of the identity of the nucleotide base at the predetermined location. The invention further provides compositions and kits for performing the subject method of the invention.
摘要翻译： 本发明涉及鉴定靶多核苷酸上预定位置的核苷酸的方法。 该方法涉及包含含有第一序列和第二序列或标签的寡核苷酸引物的单核苷酸延伸反应。 该方法还可以包括与第二序列杂交的探针或与标签相互作用的抗标签分子，其中杂交或相互作用引起可检测的信号转移，其指示在预定位置处的核苷酸碱基的身份。 本发明还提供了用于实施本发明的主题方法的组合物和试剂盒。
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公开(公告)号：US06803201B2
公开(公告)日：2004-10-12
申请号：US10056598
申请日：2002-01-24
申请人： Joseph A. Sorge , Bahram Arezi , Holly Hogrefe
发明人： Joseph A. Sorge , Bahram Arezi , Holly Hogrefe
IPC分类号： C12Q168
CPC分类号： C12Q1/6827 , C12Q1/6818 , C12Q1/6858 , C12Q2535/125 , C12Q2525/161 , C12Q2565/101
摘要： The present invention relates to a method for identifying a nucleotide at a predetermined location on a target polynucleotide. The method involves single nucleotide extension reaction comprising an oligonucleotide primer comprising a first sequence and a second sequence or a tag. The method may further comprises a probe which hybridizes to the second sequence or an anti-tag molecule which interacts with the tag, where the hybridization or interaction causes a detectable signal transfer which is indicative of the identity of the nucleotide base at the predetermined location. The invention further provides compositions and kits for performing the subject method of the invention.
摘要翻译： 本发明涉及鉴定靶多核苷酸上预定位置的核苷酸的方法。 该方法涉及包含含有第一序列和第二序列或标签的寡核苷酸引物的单核苷酸延伸反应。 该方法还可以包括与第二序列杂交的探针或与标签相互作用的抗标签分子，其中杂交或相互作用引起可检测的信号转移，其指示在预定位置处的核苷酸碱基的身份。 本发明还提供了用于实施本发明的主题方法的组合物和试剂盒。
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公开(公告)号：US20050272074A1
公开(公告)日：2005-12-08
申请号：US11100183
申请日：2005-04-06
申请人： Bahram Arezi
发明人： Bahram Arezi
CPC分类号： C12N9/1276
摘要： The present invention provides compositions and methods for high fidelity cDNA synthesis. In particular, the composition of the present invention contains a first enzyme exhibiting a reverse transcriptase activity and a second enzyme comprising a 3′-5′ exonuclease activity.
摘要翻译： 本发明提供了用于高保真cDNA合成的组合物和方法。 特别地，本发明的组合物含有显示逆转录酶活性的第一种酶和包含3'-5'核酸外切酶活性的第二种酶。
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