公开(公告)号：US08309369B2

公开(公告)日：2012-11-13

申请号：US13224174

申请日：2011-09-01

申请人： Hong Tan ,  Robert F. Zuk ,  Yushan Tan ,  Erhua Cao ,  Min Xia ,  Jun Chen

发明人： Hong Tan ,  Robert F. Zuk ,  Yushan Tan ,  Erhua Cao ,  Min Xia ,  Jun Chen

IPC分类号： G01N33/543

CPC分类号： G01N33/54366 ,  C08B37/006 ,  G01N21/6428 ,  G01N21/645 ,  G01N33/54373 ,  G01N2021/6439 ,  G01N2201/06113

摘要： This invention relates to a detection system for measuring a fluorescent signal in a fluorescent assay. The system comprises a probe having a small sensing surface bound with a fluorescent label, and a light source and a detector both mounted at the proximal side of the sensing surface of the substrate. The invention also relates to a method for detecting an analyte in a liquid sample using a probe tip having a small surface area (≦5 mm) and a high molecular weight polymer (≧1 MD) having multiple binding molecules and multiple fluorescent labels. The binding reaction is accelerated by flowing the reaction solutions laterally and moving the probe tip up and down in the reaction vessels. The invention furthers relates to a fluorescent labeling composition comprising a cross-linked Ficoll molecule having a plurality of binding molecules and a plurality of fluorescent labels.

摘要翻译： 本发明涉及一种用于在荧光测定中测量荧光信号的检测系统。 该系统包括具有与荧光标签结合的小感测表面的探针，以及安装在基底的感测表面的近侧的光源和检测器。 本发明还涉及一种使用具有小表面积（＆NlE; 5mm）的探针尖端和具有多个结合分子和多个荧光标记的高分子量聚合物（≥1MD）来检测液体样品中的分析物的方法。 通过横向流动反应溶液并在反应容器中上下移动探针尖端来加速结合反应。 本发明还涉及包含具有多个结合分子和多个荧光标记的交联Ficoll分子的荧光标记组合物。

公开(公告)号：US20110312105A1

公开(公告)日：2011-12-22

申请号：US13224174

申请日：2011-09-01

申请人： Hong Tan ,  Robert F. Zuk ,  Yushan Tan ,  Erhua Cao ,  Min Xia ,  Jun Chen

发明人： Hong Tan ,  Robert F. Zuk ,  Yushan Tan ,  Erhua Cao ,  Min Xia ,  Jun Chen

IPC分类号： G01N21/64 ,  C07K14/36 ,  C07K16/00

CPC分类号： G01N33/54366 ,  C08B37/006 ,  G01N21/6428 ,  G01N21/645 ,  G01N33/54373 ,  G01N2021/6439 ,  G01N2201/06113

摘要： This invention relates to a detection system for measuring a fluorescent signal in a fluorescent assay. The system comprises a probe having a small sensing surface bound with a fluorescent label, and a light source and a detector both mounted at the proximal side of the sensing surface of the substrate. The invention also relates to a method for detecting an analyte in a liquid sample using a probe tip having a small surface area (≦5 mm) and a high molecular weight polymer (≧1 MD) having multiple binding molecules and multiple fluorescent labels. The binding reaction is accelerated by flowing the reaction solutions laterally and moving the probe tip up and down in the reaction vessels. The invention furthers relates to a fluorescent labeling composition comprising a cross-linked Ficoll molecule having a plurality of binding molecules and a plurality of fluorescent labels.

摘要翻译： 本发明涉及一种用于在荧光测定中测量荧光信号的检测系统。 该系统包括具有与荧光标签结合的小感测表面的探针，以及安装在基底的感测表面的近侧的光源和检测器。 本发明还涉及一种使用具有小表面积（＆NlE; 5mm）的探针尖端和具有多个结合分子和多个荧光标记的高分子量聚合物（≥1MD）来检测液体样品中的分析物的方法。 通过横向流动反应溶液并在反应容器中上下移动探针尖端来加速结合反应。 本发明还涉及包含具有多个结合分子和多个荧光标记的交联Ficoll分子的荧光标记组合物。

公开(公告)号：US08597578B2

公开(公告)日：2013-12-03

申请号：US13213525

申请日：2011-08-19

申请人： Hong Tan ,  Yushan Tan ,  Erhua Cao ,  Min Xia ,  Robert F. Zuk

发明人： Hong Tan ,  Yushan Tan ,  Erhua Cao ,  Min Xia ,  Robert F. Zuk

IPC分类号： G01N21/00 ,  G01B9/02 ,  G02B6/26 ,  G02B6/36 ,  G02B6/00 ,  G01N21/63

CPC分类号： G01N21/7703 ,  G01J3/45 ,  G01N21/45 ,  G01N21/648 ,  G01N21/8507 ,  G01N2021/7779 ,  G01N2201/062 ,  G01N2201/08 ,  G02B6/262 ,  G02B6/30

摘要： The present invention is directed to an assembly for use in detecting an analyte in a sample based on thin-film spectral interference. The assembly comprises a waveguide, a monolithic substrate optically coupled to the waveguide, and a thin-film layer directly bonded to the sensing side of the monolithic substrate. The refractive index of the monolithic substrate is higher than the refractive index of the transparent material of the thin-film layer. A spectral interference between the light reflected into the waveguide from a first reflecting surface and a second reflecting surface varies as analyte molecules in a sample bind to the analyte binding molecules coated on the thin-film layer.

公开(公告)号：US20110305599A1

公开(公告)日：2011-12-15

申请号：US13213525

申请日：2011-08-19

申请人： Hong Tan ,  Yushan Tan ,  Erhua Cao ,  Min Xia ,  Robert F. Zuk

发明人： Hong Tan ,  Yushan Tan ,  Erhua Cao ,  Min Xia ,  Robert F. Zuk

IPC分类号： G01N33/53

CPC分类号： G01N21/7703 ,  G01J3/45 ,  G01N21/45 ,  G01N21/648 ,  G01N21/8507 ,  G01N2021/7779 ,  G01N2201/062 ,  G01N2201/08 ,  G02B6/262 ,  G02B6/30

摘要： The present invention is directed to an assembly for use in detecting an analyte in a sample based on thin-film spectral interference. The assembly comprises a waveguide, a monolithic substrate optically coupled to the waveguide, and a thin-film layer directly bonded to the sensing side of the monolithic substrate. The refractive index of the monolithic substrate is higher than the refractive index of the transparent material of the thin-film layer. A spectral interference between the light reflected into the waveguide from a first reflecting surface and a second reflecting surface varies as analyte molecules in a sample bind to the analyte binding molecules coated on the thin-film layer.

摘要翻译： 本发明涉及一种用于基于薄膜光谱干涉检测样品中的分析物的组件。 组件包括波导，光耦合到波导的单片基板，以及直接结合到整体式基板的感测侧的薄膜层。 单片基板的折射率高于薄膜层的透明材料的折射率。 当样品中的分析物分子结合到涂覆在薄膜层上的分析物结合分子时，从第一反射表面和第二反射表面反射到波导中的光之间的光谱干涉变化。

公开(公告)号：US07728982B2

公开(公告)日：2010-06-01

申请号：US12099751

申请日：2008-04-08

申请人： Hong Tan ,  Yushan Tan ,  Duan Jun Chen ,  Krista Leah Witte

发明人： Hong Tan ,  Yushan Tan ,  Duan Jun Chen ,  Krista Leah Witte

IPC分类号： G01B9/02

CPC分类号： G01N33/54373 ,  G01N21/7703

摘要： Apparatus and method for detecting the presence or amount or rate of binding of an analyte in a sample solution is disclosed. The apparatus includes an optical assembly having first and second reflecting surfaces separated by a distance “d” greater than 50 nm, where the first surface is formed by a layer of analyte-binding molecules, and a light source for directing a beam of light onto said first and second reflecting surface. A detector in the apparatus operates to detect a change in the thickness of the first reflecting layer resulting from binding of analyte to the analyte-binding molecules, when the assembly is placed in the solution of analyte, by detecting a shift in phase of light waves reflected from the first and second surfaces.

摘要翻译： 公开了用于检测样品溶液中分析物结合的存在或量或速率的装置和方法。 该装置包括一个光学组件，该光学组件的第一和第二反射表面间隔大于50nm的距离“d”，其中第一表面由分析物结合分子层形成，以及用于将光束引导到 所述第一和第二反射面。 该装置中的检测器用于检测由分析物与分析物结合分子结合而产生的第一反射层厚度的变化，当通过检测光波相位偏移将组件放置在分析物溶液中时 从第一和第二表面反射。

公开(公告)号：US07394547B2

公开(公告)日：2008-07-01

申请号：US10981901

申请日：2004-11-04

申请人： Hong Tan ,  Duan Jun Chen ,  Yushan Tan ,  Krista Leah Witte

发明人： Hong Tan ,  Duan Jun Chen ,  Yushan Tan ,  Krista Leah Witte

IPC分类号： G01B9/02

CPC分类号： G01N33/54373 ,  G01N21/7703

摘要： Apparatus and method for detecting the presence or amount or rate of binding of an analyte in a sample solution is disclosed. The apparatus includes an optical assembly having first and second reflecting surfaces separated by a distance “d” greater than 50 nm, where the first surface is formed by a layer of analyte-binding molecules, and a light source for directing a beam of light onto said first and second reflecting surface. A detector in the apparatus operates to detect a change in the thickness of the first reflecting layer resulting from binding of analyte to the analyte-binding molecules, when the assembly is placed in the solution of analyte, by detecting a shift in phase of light waves reflected from the first and second surfaces.

摘要翻译： 公开了用于检测样品溶液中分析物结合的存在或量或速率的装置和方法。 该装置包括一个光学组件，该光学组件的第一和第二反射表面间隔大于50nm的距离“d”，其中第一表面由分析物结合分子层形成，以及用于将光束引导到 所述第一和第二反射面。 该装置中的检测器用于检测由分析物与分析物结合分子结合而产生的第一反射层的厚度的变化，当通过检测光波相位偏移将组件放置在分析物的溶液中时 从第一和第二表面反射。

公开(公告)号：US08305585B2

公开(公告)日：2012-11-06

申请号：US12790736

申请日：2010-05-28

申请人： Hong Tan ,  Yushan Tan ,  Duan Jun Chen ,  Krista Leah Witte

发明人： Hong Tan ,  Yushan Tan ,  Duan Jun Chen ,  Krista Leah Witte

IPC分类号： G01B9/02

CPC分类号： G01N33/54373 ,  G01N21/7703

摘要： Apparatus and method for detecting the presence or amount or rate of binding of an analyte in a sample solution is disclosed. The apparatus includes an optical assembly having first and second reflecting surfaces separated by a distance “d” greater than 50 nm, where the first surface is formed by a layer of analyte-binding molecules, and a light source for directing a beam of light onto said first and second reflecting surface. A detector in the apparatus operates to detect a change in the thickness of the first reflecting layer resulting from binding of analyte to the analyte-binding molecules, when the assembly is placed in the solution of analyte, by detecting a shift in phase of light waves reflected from the first and second surfaces.

摘要翻译： 公开了用于检测样品溶液中分析物结合的存在或量或速率的装置和方法。 该装置包括光学组件，该光学组件具有分开距离d大于50nm的第一和第二反射表面，其中第一表面由分析物结合分子层形成;以及光源，用于将光束引导到所述第一 和第二反射面。 该装置中的检测器用于检测由分析物与分析物结合分子结合而产生的第一反射层的厚度的变化，当通过检测光波相位偏移将组件放置在分析物的溶液中时 从第一和第二表面反射。

公开(公告)号：US20100238453A1

公开(公告)日：2010-09-23

申请号：US12790736

申请日：2010-05-28

申请人： Hong Tan ,  Duan Jun Chen ,  Yushan Tan ,  Krista Leah Witte

发明人： Hong Tan ,  Duan Jun Chen ,  Yushan Tan ,  Krista Leah Witte

IPC分类号： G01B9/02

CPC分类号： G01N33/54373 ,  G01N21/7703

摘要： Apparatus and method for detecting the presence or amount or rate of binding of an analyte in a sample solution is disclosed. The apparatus includes an optical assembly having first and second reflecting surfaces separated by a distance “d” greater than 50 nm, where the first surface is formed by a layer of analyte-binding molecules, and a light source for directing a beam of light onto said first and second reflecting surface. A detector in the apparatus operates to detect a change in the thickness of the first reflecting layer resulting from binding of analyte to the analyte-binding molecules, when the assembly is placed in the solution of analyte, by detecting a shift in phase of light waves reflected from the first and second surfaces.

摘要翻译： 公开了用于检测样品溶液中分析物结合的存在或量或速率的装置和方法。 该装置包括一个光学组件，该光学组件的第一和第二反射表面间隔大于50nm的距离“d”，其中第一表面由分析物结合分子层形成，以及用于将光束引导到 所述第一和第二反射面。 该装置中的检测器用于检测由分析物与分析物结合分子结合而产生的第一反射层厚度的变化，当通过检测光波相位偏移将组件放置在分析物溶液中时 从第一和第二表面反射。

公开(公告)号：US20080186505A1

公开(公告)日：2008-08-07

申请号：US12099751

申请日：2008-04-08

申请人： Hong Tan ,  Duan Jun Chen ,  Yushan Tan ,  Krista Leah Witte

发明人： Hong Tan ,  Duan Jun Chen ,  Yushan Tan ,  Krista Leah Witte

IPC分类号： G01B9/02

CPC分类号： G01N33/54373 ,  G01N21/7703

摘要： Apparatus and method for detecting the presence or amount or rate of binding of an analyte in a sample solution is disclosed. The apparatus includes an optical assembly having first and second reflecting surfaces separated by a distance “d” greater than 50 nm, where the first surface is formed by a layer of analyte-binding molecules, and a light source for directing a beam of light onto said first and second reflecting surface. A detector in the apparatus operates to detect a change in the thickness of the first reflecting layer resulting from binding of analyte to the analyte-binding molecules, when the assembly is placed in the solution of analyte, by detecting a shift in phase of light waves reflected from the first and second surfaces.

公开(公告)号：US08304255B1

公开(公告)日：2012-11-06

申请号：US12369564

申请日：2009-02-11

申请人： Robert F. Zuk ,  Hong Tan

发明人： Robert F. Zuk ,  Hong Tan

IPC分类号： G01N33/543

CPC分类号： G01N33/54386

摘要： The present invention is directed immunoassay cuvettes that comprise diffusely bound and non-diffusely bound reagents for carrying out an immunoassay. The reaction and detection are carried out in the immunoassay cuvette. The immunoassay cuvette comprises a transparent front wall, a back wall, side walls, a bottom, and a top opening. The back wall of the cuvette has a substantially planar surface made of a non-porous material and comprises a capture zone having reagents non-diffusedly bound and a signal reagent zone having reagents diffusedly bound.

摘要翻译： 本发明涉及包含扩散结合和非扩散结合试剂进行免疫测定的免疫测定试管。 反应和检测在免疫测定试管中进行。 免疫测定试管包括透明的前壁，后壁，侧壁，底部和顶部开口。 试管的后壁具有由无孔材料制成的基本平坦的表面，并且包括具有非扩散结合的试剂的捕获区和具有扩散结合的试剂的信号试剂区。