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公开(公告)号：US5658446A
公开(公告)日：1997-08-19
申请号：US688351
申请日：1996-07-30
IPC分类号： G01N27/447 , G01N27/26
CPC分类号： G01N27/44704
摘要： A wide-bore capillary electrophoresis apparatus for the analysis of analyte ions is provided. The apparatus has a wide-bore capillary that has a restriction zone at one or more ends. The capillary has an inlet end and an outlet end and an opening at each of said ends. The restriction zone is capable of providing fluid communication between the wide bore and the opening at said end. The restriction zone includes a narrow bore extending to the opening and a transition zone providing gradual change of bore diameter from the wide bore to the narrow bore. The apparatus further has a buffer source to supply buffer to the inlet end of the capillary and a power supply for supplying power to drive buffer and analyte ions through the capillary. During CE, electrodes provide electrical communication between the power supply and the inlet end and the outlet end and apply a voltage differential between said ends.
摘要翻译： 提供了一种用于分析离子的分析的宽孔毛细管电泳装置。 该装置具有在一个或多个端部处具有限制区的宽孔毛细管。 毛细管具有入口端和出口端以及在每个端部处的开口。 限制区域能够在所述端部的宽孔和开口之间提供流体连通。 限制区域包括延伸到开口的窄孔和过渡区，其提供从宽孔到窄孔的孔直径的逐渐变化。 该装置还具有缓冲源，以向毛细管的入口端供应缓冲器，以及用于通过毛细管向驱动缓冲器和分析物离子供电的电源。 在CE期间，电极在电源与入口端和出口端之间提供电气连通，并在所述端部之间施加电压差。
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公开(公告)号：US5482608A
公开(公告)日：1996-01-09
申请号：US6353
申请日：1993-01-19
IPC分类号： G01N30/02 , G01N27/447 , C25B9/00
CPC分类号： G01N27/44752
摘要： A system for controlling the bulk flow rate in capillary electrophoresis employs pressure to increase velocity without unacceptably increasing plate height. Thus, the system controls bulk flow over a range of velocities, independent of the chemistry of the system. Additionally, the use of pressure under certain conditions may decrease plate height and improve resolution.
摘要翻译： 用于控制毛细管电泳中的体积流速的系统使用压力来增加速度，而不会不可接受地增加板的高度。 因此，系统在一定范围内控制体积流量，与系统的化学性无关。 另外，在某些条件下使用压力可能会降低板高度并提高分辨率。
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公开(公告)号：US06210570B1
公开(公告)日：2001-04-03
申请号：US09137607
申请日：1998-08-21
申请人： Robert R. Holloway
发明人： Robert R. Holloway
IPC分类号： B01D1508
CPC分类号： B01J20/283 , B01D15/22 , B01J20/103 , B01J20/28042 , B01J20/28078 , B01J20/28092 , B01J2220/54 , B01J2220/82 , B01J2220/84 , G01N2030/528
摘要： The present invention relates to capillary columns including a monolith and a method for preparing a capillary column including a monolith. The monolith can be prepared by a sol gel method, and in the transformation from hydrosol to hydrogel, the monolith undergoes essentially no syneresis or volume shrinkage. Thus, deleterious effects of syneresis are avoided, such as the formation of channels having large dimensions that provide a pathway of least resistance for a mobile phase to effectively bypass portions of a stationary phase. The method for preparing a column having a monolith that undergoes essentially no syneresis involves a hydrogel solution that has a relatively low concentration of SiO2, i.e. less than about 5 g/100 mL.
摘要翻译： 本发明涉及包括整料的毛细管柱和用于制备包括整料的毛细管柱的方法。 整料可以通过溶胶凝胶法制备，并且在从水溶胶转化成水凝胶的过程中，整料基本上不经脱水收缩或体积收缩。 因此，避免了脱水收缩的有害作用，例如形成具有大尺寸的通道，其提供流动相的电阻最小的途径以有效地绕过固定相的部分。 制备具有基本上不进行脱水收缩的整料的柱的方法包括具有相对低的SiO 2浓度（即小于约5g / 100mL）的水凝胶溶液。
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公开(公告)号：US5415747A
公开(公告)日：1995-05-16
申请号：US107393
申请日：1993-08-16
申请人： Robert R. Holloway
发明人： Robert R. Holloway
IPC分类号： G01N27/447 , G01N27/26
CPC分类号： G01N27/44752
摘要： A method and apparatus for controlling the velocity of electroosmotic flow (EOF) in high performance capillary electrophoresis (HPCE) are provided. The interior surface of the capillary tube is coated with a zwitterionic coating, such that the pH range within which EOF velocity may be controlled is broadened. The zwitterionic coating involves covalent, ionic or adsorptive binding of a zwitterionic species such as phosphoryl choline to the capillary wall.
摘要翻译： 提供了一种用于控制高效毛细管电泳（HPCE）中电渗流速度（EOF）的方法和装置。 毛细管的内表面涂有两性离子涂层，使得可以控制EOF速度的pH范围变宽。 两性离子涂层涉及两性离子物质如磷酰胆碱与毛细管壁共价，离子或吸附结合。
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5.发明授权Chemically sensitive, dimensionally-stable organosilicon material composition and techniques 失效化学敏感，尺寸稳定的有机硅材料组成和技术
公开(公告)号：US5194391A
公开(公告)日：1993-03-16
申请号：US720983
申请日：1991-06-25
CPC分类号： G01N31/22 , C08K13/02 , Y10T436/207497
摘要： A dimensionally-stable organosilicon material composition and method for producing the material composition including non-crosslinkable, continuous phase silicone with silica filler material dispersed therethrough, and having dissolved therein a variably-radiative material (e.g., ruthenium dye) responsive to the concentration of a selected analyte (e.g., oxygen), and the products of a reaction between water and a modifier material selected for establishing the sensitivity of said variably-radiative material to the selected analyte.
摘要翻译： 一种尺寸稳定的有机硅材料组合物和用于制备材料组合物的方法，所述组合物包括分散在其中的二氧化硅填料的不可交联连续相硅氧烷，并且其中溶解有可变辐射材料（例如钌染料） 选择的分析物（例如氧气）以及水和选择用于确定所述可变辐射材料对所选择的分析物的灵敏度的改性剂材料之间的反应产物。
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公开(公告)号：US5070158A
公开(公告)日：1991-12-03
申请号：US274626
申请日：1988-11-22
申请人： Robert R. Holloway , Teddy T. Kiang
发明人： Robert R. Holloway , Teddy T. Kiang
IPC分类号： C07F7/18 , C07F15/00 , C08G77/388 , C08K5/54
CPC分类号： C08G77/388 , C07F15/0053 , C07F7/1896 , C08K5/54
摘要： Disclosed herein is a method of labeling room temperature vulcanizable silicon polymers at the "T" junction branch points by utilizing cross-linkers of the formula D-SiX.sub.3, wherein X is a leaving group and D is a complex organic molecule useful as a dye, fluorophore, or indicator.
摘要翻译： 本文公开了通过利用式D-SiX 3的交联剂在“T”结分支点处标记室温可硫化硅聚合物的方法，其中X是离去基团，D是可用作染料的复合有机分子， 荧光团或指示剂。
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公开(公告)号：US5167783A
公开(公告)日：1992-12-01
申请号：US632278
申请日：1990-12-20
申请人： Robert R. Holloway
发明人： Robert R. Holloway
IPC分类号： G01N27/447
CPC分类号： G01N27/44752 , G01N27/44747
摘要： Capillary tubes useful for performing capillary gel electrophoresis separation techniques are prepared by employing prepolymers having one or more terminal functional groups that allow for final polymerization. Use of said prepolymers significantly reduces the amount of shrinkage upon polymerization of said prepolymers to form the electrophoretic gel. The analytical media exhibits strong structural integrity, uniformity, and adheres well to the capillary tubes.
摘要翻译： 可用于进行毛细管凝胶电泳分离技术的毛细管通过使用具有一个或多个允许最终聚合的末端官能团的预聚物来制备。 所述预聚物的使用显着降低聚合所述预聚物以形成电泳凝胶的收缩量。 分析介质表现出强大的结构完整性，均匀性，并且很好地粘附在毛细管上。
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公开(公告)号：US5110439A
公开(公告)日：1992-05-05
申请号：US752709
申请日：1991-08-30
申请人： Robert R. Holloway
发明人： Robert R. Holloway
IPC分类号： B01D57/02 , G01N27/447
CPC分类号： G01N27/44752
摘要： A polyacrylamide gel electrophoresis column includes a fused-silica tube, a polyacrylamide gel matrix and a polydimethysiloxane layer radially between and covalently attached to both the tube and the gel matrix. The method for forming this column involves covalently binding polyorganosiloxane to the inner wall of the tube, covalently attaching organosilane termini to the organosiloxane layer, and polymerizing acrylamide so as to incorporate the organosilane termini into the resulting polyacrylamide gel matrix. Buffer ions are then drawn into the column under an electric field of about 1000 volts per centimeter, and, at the same time, different reaction product ions are removed. The polyorganosiloxane layer stretches as the polyacrylamide shrinks during polymerization so that the organosiloxane is at least twice as thick as it would be without the gel matrix attached. The process yields a gel matrix which is securely attached to the tube, void-free, and under minimal mechanical stress. As a result, the column can withstand relatively high-voltage pre-electrophoresis and electrophoresis procedures.
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9.发明授权
公开(公告)号：US5057277A
公开(公告)日：1991-10-15
申请号：US259015
申请日：1988-10-17
摘要： A dimensionally-stable organosilicon material composition and method for producing the material composition including noncrosslinkable, continuous phase silicone with silica filler material dispersed therethorugh, and having dissolved therein a variably-radiative material (e.g., ruthenium dye) responsive to the concentration of a selected analyte (e.g., oxygen), and the products of a reaction between water and a modifier material selected for establishing the sensitivity of said variably-radiative material to the selected analyte.
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