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公开(公告)号：US08518674B2
公开(公告)日：2013-08-27
申请号：US12893469
申请日：2010-09-29
申请人： Howard Glenn Damude , Peter John Gillies , Daniel Joseph Macool , Stephen K. Picataggio , Dana M. Walters Pollak , James John Ragghianti , Zhixiong Xue , Narendra S. Yadav , Hongxiang Zhang , Quinn Qun Zhu
发明人： Howard Glenn Damude , Peter John Gillies , Daniel Joseph Macool , Stephen K. Picataggio , Dana M. Walters Pollak , James John Ragghianti , Zhixiong Xue , Narendra S. Yadav , Hongxiang Zhang , Quinn Qun Zhu
CPC分类号： C12P7/6427 , A23D9/00 , A23K20/158 , A23L33/12 , A23V2002/00 , C12N1/16 , C12N9/0083 , C12N9/1029 , C12N15/52 , C12N15/746 , C12N15/815 , C12P7/6472 , A23V2250/187 , A23V2200/30
摘要： Engineered strains of the oleaginous yeast Yarrowia lipolytica capable of producing greater than 25% eicosapentaenoic acid (EPA, an ω-3 polyunsaturated fatty acid) in the total oil fraction are described. These strains comprise various chimeric genes expressing heterologous desaturases, elongases and acyltransferases and optionally comprise various native desaturase and acyltransferase knockouts to enable synthesis and high accumulation of EPA. Production host cells are claimed, as are methods for producing EPA within said host cells.
摘要翻译： 描述了能够在总油馏分中产生大于25％二十碳五烯酸（EPA，ω-3多不饱和脂肪酸）的含油酵母解脂耶氏酵母解脂耶氏酵母菌的工程菌株。 这些菌株包括表达异源去饱和酶，延伸酶和酰基转移酶的各种嵌合基因，并且任选地包含各种天然去饱和酶和酰基转移酶敲除，以使EPA能够合成和高度积累。 要求生产宿主细胞，以及在所述宿主细胞内生产EPA的方法。
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公开(公告)号：US08420892B2
公开(公告)日：2013-04-16
申请号：US12725482
申请日：2010-03-17
CPC分类号： C12N15/8247 , C11B1/025 , C12N9/1029 , C12P7/6427 , C12P7/6463 , C12P7/6472
摘要： Isolated nucleic acid fragments and recombinant constructs comprising such fragments encoding delta-9 elongases along with a method of making long-chain polyunsaturated fatty acids (PUFAs) using these delta-9 elongases in plants.
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3.发明申请公开(公告)号：US20110055973A1
公开(公告)日：2011-03-03
申请号：US12944539
申请日：2010-11-11
申请人： Howard Glenn Damude , Zhixiong Xue , Quinn Qun Zhu
发明人： Howard Glenn Damude , Zhixiong Xue , Quinn Qun Zhu
IPC分类号： A01H1/00
CPC分类号： C12N15/8247 , A23K20/158 , A23L33/12 , C12N9/0083
摘要： Isolated nucleic acid fragments and recombinant constructs comprising such fragments encoding delta-8 desaturases along with a method of making long-chain polyunsaturated fatty acids (PUFAs) using these delta-8 desaturases in plants and oleaginous yeast.
摘要翻译： 分离的核酸片段和包含编码δ-8去饱和酶的片段的重组构建物以及使用这些δ-8去饱和酶在植物和油质酵母中制备长链多不饱和脂肪酸（PUFA）的方法。
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公开(公告)号：US07588931B2
公开(公告)日：2009-09-15
申请号：US11264784
申请日：2005-11-01
申请人： Howard Glenn Damude , Peter John Gillies , Daniel Joseph Macool , Stephen K. Picataggio , Dana M. Walters Pollak , James John Ragghianti , Zhixiong Xue , Narendra S. Yadav , Hongxiang Zhang , Quinn Qun Zhu
发明人： Howard Glenn Damude , Peter John Gillies , Daniel Joseph Macool , Stephen K. Picataggio , Dana M. Walters Pollak , James John Ragghianti , Zhixiong Xue , Narendra S. Yadav , Hongxiang Zhang , Quinn Qun Zhu
CPC分类号： C12P7/6427 , A23D9/00 , A23K20/158 , A23L33/12 , A23V2002/00 , C12N1/16 , C12N9/0083 , C12N9/1029 , C12N15/52 , C12N15/746 , C12N15/815 , C12P7/6472 , A23V2250/187 , A23V2200/30
摘要： Engineered strains of the oleaginous yeast Yarrowia lipolytica capable of producing greater than 10% arachidonic acid (ARA, an ω-6 polyunsaturated fatty acid) in the total oil fraction are described. These strains comprise various chimeric genes expressing heterologous desaturases, elongases and acyltransferases, and optionally comprise various native desaturase and acyltransferase knockouts to enable synthesis and high accumulation of ARA. Production host cells are claimed, as are methods for producing ARA within said host cells.
摘要翻译： 描述了能够在总油馏分中产生大于10％花生四烯酸（ARA，ω-6多不饱和脂肪酸）的含油酵母解脂耶氏酵母解脂耶氏酵母菌的工程菌株。 这些菌株包含表达异源去饱和酶，延伸酶和酰基转移酶的各种嵌合基因，并且任选地包含各种天然去饱和酶和酰基转移酶敲除，以使ARA能够合成和高度积累。 要求生产宿主细胞，以及在所述宿主细胞内产生ARA的方法。
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5.发明申请Delta-9-Elongases and their Use in Making Polyunsaturated Fatty Acids 有权Delta-9-延长酶及其在制备多不饱和脂肪酸中的应用公开(公告)号：US20090119795A1
公开(公告)日：2009-05-07
申请号：US11992899
申请日：2006-11-16
CPC分类号： C12N15/8247 , C11B1/025 , C12N9/1029 , C12P7/6427 , C12P7/6463 , C12P7/6472
摘要： Isolated nucleic acid fragments and recombinant constructs comprising such fragments encoding delta-9 elongases along with a method of making long-chain polyunsaturated fatty acids (PUFAs) using these delta-9 elongases in plants.
摘要翻译： 分离的核酸片段和包含编码Δ-9延伸酶的这种片段的重组构建物以及在植物中使用这些δ-9延长酶制备长链多不饱和脂肪酸（PUFA）的方法。
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公开(公告)号：US07879591B2
公开(公告)日：2011-02-01
申请号：US12485141
申请日：2009-06-16
CPC分类号： C12P7/6472 , A23K20/158 , A23L33/12 , A23V2002/00 , C12N9/0083 , C12N9/1029 , C12N15/52 , C12P7/6427 , A23V2250/187 , A23V2200/30
摘要： Lysophosphatidic acid acyltransferase [“LPAAT”] participates in the second step of oil biosynthesis and is expected to play a key role in altering the quantity of long-chain polyunsaturated fatty acids [“LC-PUFAs”] produced in oils of oleaginous organisms. An LPAAT isolated from Mortierella alpina [“MaLPAAT1”] that is suitable for use in the manufacture of oils enriched in LC-PUFAs in oleaginous organisms is disclosed. Most desirably, the substrate specificity of the instant MaLPAAT1 will be particularly useful to enable increased C18 to C20 elongation conversion efficiency and increased Δ4 desaturation conversion efficiency in recombinant host cells producing LC-PUFAs.
摘要翻译： 溶血磷脂酰基转移酶[“LPAAT”]参与了油生物合成的第二步，预计在改变含油生物油中产生的长链多不饱和脂肪酸（“LC-PUFA”）的量方面起关键作用。 公开了一种适用于制造含油生物中富含LC-PUFA的油的高山被孢霉[“MaLPAAT1”]分离的LPAAT。 最令人满意的是，本发明的MaLPAAT1的底物特异性将特别有用，以便能够在产生LC-PUFA的重组宿主细胞中提高C18至C20延伸转换效率和增加和降低转化效率。
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7.发明申请公开(公告)号：US20100175148A1
公开(公告)日：2010-07-08
申请号：US12725482
申请日：2010-03-17
IPC分类号： C12P7/64 , C07H21/04 , C12N9/02 , C12N15/82 , C12N5/10 , C12N15/63 , A01H1/00 , A01H5/10 , A01H5/00
CPC分类号： C12N15/8247 , C11B1/025 , C12N9/1029 , C12P7/6427 , C12P7/6463 , C12P7/6472
摘要： Isolated nucleic acid fragments and recombinant constructs comprising such fragments encoding delta-9 elongases along with a method of making long-chain polyunsaturated fatty acids (PUFAs) using these delta-9 elongases in plants.
摘要翻译： 分离的核酸片段和包含编码Δ-9延伸酶的这种片段的重组构建物以及在植物中使用这些δ-9延长酶制备长链多不饱和脂肪酸（PUFA）的方法。
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公开(公告)号：US20090325265A1
公开(公告)日：2009-12-31
申请号：US12485141
申请日：2009-06-16
CPC分类号： C12P7/6472 , A23K20/158 , A23L33/12 , A23V2002/00 , C12N9/0083 , C12N9/1029 , C12N15/52 , C12P7/6427 , A23V2250/187 , A23V2200/30
摘要： Lysophosphatidic acid acyltransferase [“LPAAT”] participates in the second step of oil biosynthesis and is expected to play a key role in altering the quantity of long-chain polyunsaturated fatty acids [“LC-PUFAs”] produced in oils of oleaginous organisms. An LPAAT isolated from Mortierella alpina [“MaLPAAT1”] that is suitable for use in the manufacture of oils enriched in LC-PUFAs in oleaginous organisms is disclosed. Most desirably, the substrate specificity of the instant MaLPAAT1 will be particularly useful to enable increased C18 to C20 elongation conversion efficiency and increased Δ4 desaturation conversion efficiency in recombinant host cells producing LC-PUFAs.
摘要翻译： 溶血磷脂酰基转移酶[“LPAAT”]参与了油生物合成的第二步，预计在改变含油生物油中产生的长链多不饱和脂肪酸（“LC-PUFA”）的量方面起关键作用。 公开了一种适用于制造含油生物中富含LC-PUFA的油的高山被孢霉[“MaLPAAT1”]分离的LPAAT。 最令人满意的是，本发明的MaLPAAT1的底物特异性将特别有用，以便在产生LC-PUFA的重组宿主细胞中提高C18至C20延伸转换效率和增加Delta4去饱和转化效率。
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公开(公告)号：US20090311380A1
公开(公告)日：2009-12-17
申请号：US12466072
申请日：2009-05-14
申请人： Howard Glenn Damude , Peter John Gillies , Daniel Joseph Macool , Stephen K. Picataggio , James John Ragghianti , John E. Seip , Zhixiong Xue , Narendra S. Yadav , Hongxiang Zhang , Quinn Qun Zhu
发明人： Howard Glenn Damude , Peter John Gillies , Daniel Joseph Macool , Stephen K. Picataggio , James John Ragghianti , John E. Seip , Zhixiong Xue , Narendra S. Yadav , Hongxiang Zhang , Quinn Qun Zhu
CPC分类号： C12P7/6427 , A23D9/00 , A23K20/158 , A23L33/12 , A23V2002/00 , C12N1/16 , C12N9/0083 , C12N9/1029 , C12N15/52 , C12N15/746 , C12N15/815 , C12P7/6472 , A23V2250/187 , A23V2200/30
摘要： An engineered strain of the oleaginous yeast Yarrowia lipolytica capable of producing greater than 5.6% docosahexaenoic acid acid (DHA, an w-3 polyunsaturated fatty acid) in the total oil fraction is described. This strain comprises various chimeric genes expressing heterologous desaturases, elongases and acyltransferases and optionally comprises various native desaturase and acyltransferase knockouts to enable synthesis and high accumulation of DHA. Production host cells are claimed, as are methods for producing DHA within said host cells.
摘要翻译： 描述了能够在总油馏分中产生大于5.6％二十二碳六烯酸（DHA，w-3多不饱和脂肪酸）的含油酵母解脂耶氏酵母解脂耶氏酵母的改造菌株。 该菌株包含表达异源去饱和酶，延长酶和酰基转移酶的各种嵌合基因，并且任选地包含各种天然去饱和酶和酰基转移酶敲除，以使得能够合成和高浓度的DHA。 要求生产宿主细胞，以及在所述宿主细胞内产生DHA的方法。
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10.发明申请公开(公告)号：US20070271632A1
公开(公告)日：2007-11-22
申请号：US11748637
申请日：2007-05-15
CPC分类号： C12N9/0083
摘要： The present invention relates to a Δ5 desaturase, which has the ability to convert dihomo-γ-linolenic acid (DGLA; 20:3 ω-6) to arachidonic acid (ARA; 20:4 ω-6) and/or eicosatetraenoic acid (ETA; 20:4 ω-3) to eicosapentaenoic acid (EPA; 20:5 ω-3). Isolated nucleic acid fragments and recombinant constructs comprising such fragments encoding Δ5 desaturase along with a method of making long chain polyunsaturated fatty acids (PUFAs) using this Δ5 desaturase in oleaginous yeast are disclosed.
摘要翻译： 本发明涉及一种Delta5去饱和酶，其具有将二焦γ-亚麻酸（DGLA; 20：3ω-6）转化为花生四烯酸（ARA; 20：4ω-6）和/或二十碳四烯酸（ ETA; 20：4ω-3）至二十碳五烯酸（EPA; 20：5ω-3）。 公开了分离的核酸片段和包含编码Δ5去饱和酶的片段的重组构建物，以及在含油酵母中使用该Delta5去饱和酶制备长链多不饱和脂肪酸（PUFAs）的方法。
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