公开(公告)号：US20160017416A1

公开(公告)日：2016-01-21

申请号：US14798762

申请日：2015-07-14

Applicant: ILLUMINA, INC

Inventor： Boyan BOYANOV ,  Jeffrey G. MANDELL ,  Jingwei BAI ,  Kevin L. GUNDERSON ,  Cheng-Yao CHEN ,  Michel Perbost

IPC: C12Q1/68 ,  G01N27/414

CPC classification number: G01N27/4145 ,  B01J2219/00653 ,  B01J2219/00722 ,  B82Y15/00 ,  C12Q1/6825 ,  C12Q1/6869 ,  C12Q2525/197 ,  C12Q2535/122 ,  C12Q2563/113 ,  C12Q2563/116 ,  C12Q2565/107 ,  C12Q2565/607 ,  C12Q2565/629 ,  C12Q2521/101 ,  C12Q2523/307 ,  C12Q2523/319

Abstract: A method of nucleic acid sequencing. The method can include the steps of (a) providing a polymerase tethered to a solid support charge sensor; (b) providing one or more nucleotides, whereby the presence of the nucleotide can be detected by the charge sensor; and (c) detecting incorporation of the nucleotide into a nascent strand complementary to a template nucleic acid.

Abstract translation: 核酸测序方法。 该方法可以包括以下步骤：（a）提供拴在固体支持物电荷传感器上的聚合酶; （b）提供一个或多个核苷酸，由此可以通过电荷传感器检测核苷酸的存在; 和（c）检测核苷酸掺入到与模板核酸互补的新生链中。

公开(公告)号：US20200348257A1

公开(公告)日：2020-11-05

申请号：US16826035

申请日：2020-03-20

Applicant: ILLUMINA, INC.

Inventor： Boyan BOYANOV ,  Jeffrey G. MANDELL ,  Jingwei BAI ,  Kevin L. GUNDERSON ,  Cheng-Yao CHEN ,  Michel PERBOST

IPC: G01N27/414 ,  C12Q1/6869 ,  C12Q1/6825

Abstract: A kit includes an array of charge sensors attached to a solid-phase substrate , wherein individual charge sensors in the array respectively comprise a channel and a polymerase and are located at different addressable locations on the solid-phase substrate; and a liquid comprising a plurality of different nucleic acids, each of the different nucleic acids including a unique label to be detected by a charge sensor of the array.

公开(公告)号：US20180112265A1

公开(公告)日：2018-04-26

申请号：US15839795

申请日：2017-12-12

Applicant: ILLUMINA, INC.

Inventor： Boyan BOYANOV ,  Jeffrey G. MANDELL ,  Jingwei BAI ,  Kevin L. GUNDERSON ,  Cheng-Yao CHEN ,  Michel PERBOST

IPC: C12Q1/6874 ,  G01N27/414 ,  C12Q1/6869

Abstract: A method of nucleic acid sequencing. The method can include the steps of (a) providing a polymerase tethered to a solid support charge sensor; (b) providing one or more nucleotides, whereby the presence of the nucleotide can be detected by the charge sensor; and (c) detecting incorporation of the nucleotide into a nascent strand complementary to a template nucleic acid.