公开(公告)号：US20250010263A1

公开(公告)日：2025-01-09

申请号：US18755363

申请日：2024-06-26

Applicant: ILLUMINA, INC.

Inventor： Boyan BOYANOV ,  Rean Silke MUSA ,  Anthony FLANNERY ,  Sergio PEISAJOVICH

IPC: B01J19/00

Abstract: An microreactor array is provided that includes a plurality of microreactors in a substrate. The plurality of microreactors is in fluid communication with a common fluid delivery channel. Each of the microreactors includes an electrode configured to generate one or more gaseous bubbles that can block reagent access to selected microreactors. A method for selective reactions in a microreactor array is also provide.

公开(公告)号：US20240319165A1

公开(公告)日：2024-09-26

申请号：US18575254

申请日：2022-11-09

Applicant: ILLUMINA, INC.

Inventor： Xu LIU ,  Sharis MINASSIAN ,  Boyan BOYANOV ,  Rean Silke MUSA ,  Arvin EMADI

IPC: G01N33/487

CPC classification number: G01N33/48721

Abstract: Systems for sequencing biopolymers and methods of manufacturing the systems are disclosed. In one example, such a system may include an application specific integrated circuit (ASIC) layer, a post array layer beneath the AISC layer, and a nanopore layer above the ASIC layer. The ASIC layer is formed by building active circuitry on a front side of a semiconductor wafer and polishing a back side of the semiconductor wafer. The post array layer is formed by etching a front side of a support substrate and the post array layer provides mechanical support to the ASIC layer. The nanopore layer contains membrane and nanopores. The membrane inhibits passage of water-soluble molecules and the nanopores permit passage of water-soluble molecules. In some embodiments, the system may have short through-substrate vias extending through the ASIC layer. In some embodiments, wafer bonding processes may be used when fabricating the system.

公开(公告)号：US20230314326A1

公开(公告)日：2023-10-05

申请号：US18313015

申请日：2023-05-05

Applicant: ILLUMINA, INC.

Inventor： Tracy Helen FUNG ,  Xiuyu CAI ,  Lisa KWOK ,  Hai TRAN ,  Kevan SAMIEE ,  Liangliang QIANG ,  Boyan BOYANOV

IPC: G01N21/64 ,  G01N15/14 ,  G01N21/77 ,  B01L3/00

CPC classification number: G01N21/6454 ,  B01L3/502715 ,  G01N15/1436 ,  G01N21/6486 ,  G01N21/7703 ,  G01N2021/7763

Abstract: An example sensor includes a flow cell, a detection device, and a controller. The flow cell includes a passivation layer having opposed surfaces and a reaction site at a first of the opposed surfaces. The flow cell also includes a lid operatively connected to the passivation layer to partially define a flow channel between the lid and the reaction site. The detection device is in contact with a second of the opposed surfaces of the passivation layer, and includes an embedded metal layer that is electrically isolated from other detection circuitry of the detection device. The controller is to ground the embedded metal layer.

公开(公告)号：US20160017416A1

公开(公告)日：2016-01-21

申请号：US14798762

申请日：2015-07-14

Applicant: ILLUMINA, INC

Inventor： Boyan BOYANOV ,  Jeffrey G. MANDELL ,  Jingwei BAI ,  Kevin L. GUNDERSON ,  Cheng-Yao CHEN ,  Michel Perbost

IPC: C12Q1/68 ,  G01N27/414

CPC classification number: G01N27/4145 ,  B01J2219/00653 ,  B01J2219/00722 ,  B82Y15/00 ,  C12Q1/6825 ,  C12Q1/6869 ,  C12Q2525/197 ,  C12Q2535/122 ,  C12Q2563/113 ,  C12Q2563/116 ,  C12Q2565/107 ,  C12Q2565/607 ,  C12Q2565/629 ,  C12Q2521/101 ,  C12Q2523/307 ,  C12Q2523/319

Abstract: A method of nucleic acid sequencing. The method can include the steps of (a) providing a polymerase tethered to a solid support charge sensor; (b) providing one or more nucleotides, whereby the presence of the nucleotide can be detected by the charge sensor; and (c) detecting incorporation of the nucleotide into a nascent strand complementary to a template nucleic acid.

Abstract translation: 核酸测序方法。 该方法可以包括以下步骤：（a）提供拴在固体支持物电荷传感器上的聚合酶; （b）提供一个或多个核苷酸，由此可以通过电荷传感器检测核苷酸的存在; 和（c）检测核苷酸掺入到与模板核酸互补的新生链中。

公开(公告)号：US20200348257A1

公开(公告)日：2020-11-05

申请号：US16826035

申请日：2020-03-20

Applicant: ILLUMINA, INC.

Inventor： Boyan BOYANOV ,  Jeffrey G. MANDELL ,  Jingwei BAI ,  Kevin L. GUNDERSON ,  Cheng-Yao CHEN ,  Michel PERBOST

IPC: G01N27/414 ,  C12Q1/6869 ,  C12Q1/6825

Abstract: A kit includes an array of charge sensors attached to a solid-phase substrate , wherein individual charge sensors in the array respectively comprise a channel and a polymerase and are located at different addressable locations on the solid-phase substrate; and a liquid comprising a plurality of different nucleic acids, each of the different nucleic acids including a unique label to be detected by a charge sensor of the array.

公开(公告)号：US20180112265A1

公开(公告)日：2018-04-26

申请号：US15839795

申请日：2017-12-12

Applicant: ILLUMINA, INC.

Inventor： Boyan BOYANOV ,  Jeffrey G. MANDELL ,  Jingwei BAI ,  Kevin L. GUNDERSON ,  Cheng-Yao CHEN ,  Michel PERBOST

IPC: C12Q1/6874 ,  G01N27/414 ,  C12Q1/6869

Abstract: A method of nucleic acid sequencing. The method can include the steps of (a) providing a polymerase tethered to a solid support charge sensor; (b) providing one or more nucleotides, whereby the presence of the nucleotide can be detected by the charge sensor; and (c) detecting incorporation of the nucleotide into a nascent strand complementary to a template nucleic acid.