公开(公告)号：US20160032377A1

公开(公告)日：2016-02-04

申请号：US14776284

申请日：2013-03-14

Applicant: ILLUMINA, INC.

Inventor： Cheng-Yao CHEN ,  Molly HE ,  Erin BOMATI ,  Sergio PEISAJOVICH

IPC: C12Q1/68 ,  C12N9/12

Abstract: Presented herein are polymerase enzymes for improved incorporation of nucleotide analogues, in particular nucleotides which are modified at the 3′ sugar hydroxyl, as well as methods and kits using the same.

公开(公告)号：US20200348257A1

公开(公告)日：2020-11-05

申请号：US16826035

申请日：2020-03-20

Applicant: ILLUMINA, INC.

Inventor： Boyan BOYANOV ,  Jeffrey G. MANDELL ,  Jingwei BAI ,  Kevin L. GUNDERSON ,  Cheng-Yao CHEN ,  Michel PERBOST

IPC: G01N27/414 ,  C12Q1/6869 ,  C12Q1/6825

Abstract: A kit includes an array of charge sensors attached to a solid-phase substrate , wherein individual charge sensors in the array respectively comprise a channel and a polymerase and are located at different addressable locations on the solid-phase substrate; and a liquid comprising a plurality of different nucleic acids, each of the different nucleic acids including a unique label to be detected by a charge sensor of the array.

公开(公告)号：US20180112265A1

公开(公告)日：2018-04-26

申请号：US15839795

申请日：2017-12-12

Applicant: ILLUMINA, INC.

Inventor： Boyan BOYANOV ,  Jeffrey G. MANDELL ,  Jingwei BAI ,  Kevin L. GUNDERSON ,  Cheng-Yao CHEN ,  Michel PERBOST

IPC: C12Q1/6874 ,  G01N27/414 ,  C12Q1/6869

Abstract: A method of nucleic acid sequencing. The method can include the steps of (a) providing a polymerase tethered to a solid support charge sensor; (b) providing one or more nucleotides, whereby the presence of the nucleotide can be detected by the charge sensor; and (c) detecting incorporation of the nucleotide into a nascent strand complementary to a template nucleic acid.

公开(公告)号：US20160017416A1

公开(公告)日：2016-01-21

申请号：US14798762

申请日：2015-07-14

Applicant: ILLUMINA, INC

Inventor： Boyan BOYANOV ,  Jeffrey G. MANDELL ,  Jingwei BAI ,  Kevin L. GUNDERSON ,  Cheng-Yao CHEN ,  Michel Perbost

IPC: C12Q1/68 ,  G01N27/414

CPC classification number: G01N27/4145 ,  B01J2219/00653 ,  B01J2219/00722 ,  B82Y15/00 ,  C12Q1/6825 ,  C12Q1/6869 ,  C12Q2525/197 ,  C12Q2535/122 ,  C12Q2563/113 ,  C12Q2563/116 ,  C12Q2565/107 ,  C12Q2565/607 ,  C12Q2565/629 ,  C12Q2521/101 ,  C12Q2523/307 ,  C12Q2523/319

Abstract: A method of nucleic acid sequencing. The method can include the steps of (a) providing a polymerase tethered to a solid support charge sensor; (b) providing one or more nucleotides, whereby the presence of the nucleotide can be detected by the charge sensor; and (c) detecting incorporation of the nucleotide into a nascent strand complementary to a template nucleic acid.

Abstract translation: 核酸测序方法。 该方法可以包括以下步骤：（a）提供拴在固体支持物电荷传感器上的聚合酶; （b）提供一个或多个核苷酸，由此可以通过电荷传感器检测核苷酸的存在; 和（c）检测核苷酸掺入到与模板核酸互补的新生链中。