公开(公告)号：US11512348B2

公开(公告)日：2022-11-29

申请号：US15752192

申请日：2016-08-12

Applicant: Illumina, Inc.

Inventor： Jeffrey G. Mandell ,  Lisa Kwok

IPC: C12Q1/6874 ,  G01R33/09 ,  G01R33/12 ,  B01L3/00 ,  G01N27/327 ,  C12Q1/6869 ,  G01N33/543

Abstract: Sequencing-by-synthesis (SBS) method is provided that includes providing a detection apparatus that includes an array of magnetically-responsive sensors. Each of the magnetically-responsive sensors is located proximate to a respective designated space to detect a magnetic property therefrom. The detection apparatus also includes a plurality of nucleic acid template strands located within corresponding designated spaces. The method also includes conducting a plurality of SBS events to grow a complementary strand by incorporating nucleotides along each template strand. At least some of the nucleotides are attached to corresponding magnetic particles having respective magnetic properties. Each of the plurality of SBS events includes detecting changes in electrical resistance at the magnetically-responsive sensors caused by the respective magnetic properties of the magnetic particles. The method also includes determining genetic characteristics of the complementary strands based on the detected changes in electrical resistance.

公开(公告)号：US12241835B2

公开(公告)日：2025-03-04

申请号：US18313015

申请日：2023-05-05

Applicant: ILLUMINA, INC.

Inventor： Tracy Helen Fung ,  Xiuyu Cai ,  Lisa Kwok ,  Hai Tran ,  Kevan Samiee ,  Liangliang Qiang ,  Boyan Boyanov

IPC: G01N21/64 ,  B01L3/00 ,  G01N15/1434 ,  G01N21/77

Abstract: An example sensor includes a flow cell, a detection device, and a controller. The flow cell includes a passivation layer having opposed surfaces and a reaction site at a first of the opposed surfaces. The flow cell also includes a lid operatively connected to the passivation layer to partially define a flow channel between the lid and the reaction site. The detection device is in contact with a second of the opposed surfaces of the passivation layer, and includes an embedded metal layer that is electrically isolated from other detection circuitry of the detection device. The controller is to ground the embedded metal layer.

公开(公告)号：US20250164399A1

公开(公告)日：2025-05-22

申请号：US19027132

申请日：2025-01-17

Applicant: Illumina, Inc.

Inventor： Tracy Helen Fung ,  Xiuyu Cai ,  Lisa Kwok ,  Hai Tran ,  Kevan Samiee ,  Liangliang Qiang ,  Boyan Boyanov

IPC: G01N21/64 ,  B01L3/00 ,  G01N15/1434 ,  G01N21/77

Abstract: An example sensor includes a flow cell, a detection device, and a controller. The flow cell includes a passivation layer having opposed surfaces and a reaction site at a first of the opposed surfaces. The flow cell also includes a lid operatively connected to the passivation layer to partially define a flow channel between the lid and the reaction site. The detection device is in contact with a second of the opposed surfaces of the passivation layer, and includes an embedded metal layer that is electrically isolated from other detection circuitry of the detection device. The controller is to ground the embedded metal layer.

公开(公告)号：US11680906B2

公开(公告)日：2023-06-20

申请号：US16607354

申请日：2018-04-19

Applicant: ILLUMINA, INC.

Inventor： Tracy Helen Fung ,  Xiuyu Cai ,  Lisa Kwok ,  Hai Tran ,  Kevan Samiee ,  Liangliang Qiang ,  Boyan Boyanov

IPC: G01N21/64 ,  G01N21/77 ,  B01L3/00 ,  G01N15/14

CPC classification number: G01N21/6454 ,  B01L3/502715 ,  G01N15/1436 ,  G01N21/6486 ,  G01N21/7703 ,  G01N2021/7763

Abstract: An example sensor includes a flow cell, a detection device, and a controller. The flow cell includes a passivation layer having opposed surfaces and a reaction site at a first of the opposed surfaces. The flow cell also includes a lid operatively connected to the passivation layer to partially define a flow channel between the lid and the reaction site. The detection device is in contact with a second of the opposed surfaces of the passivation layer, and includes an embedded metal layer that is electrically isolated from other detection circuitry of the detection device. The controller is to ground the embedded metal layer.

公开(公告)号：US20230082589A1

公开(公告)日：2023-03-16

申请号：US17980341

申请日：2022-11-03

Applicant: Illumina, Inc.

Inventor： Jeffrey G. Mandell ,  Lisa Kwok

IPC: C12Q1/6874 ,  G01R33/09 ,  G01R33/12 ,  B01L3/00 ,  G01N27/327 ,  C12Q1/6869 ,  G01N33/543

Abstract: Some examples herein provide methods for determining a sequence of a nucleic acid template hybridized to a complementary strand. A polymerase coupled to a magnetically-responsive sensor may capture the nucleic acid template hybridized to the complementary strand. The polymerase and the captured nucleic acid template hybridized to the complementary strand may be contacted with a first fluid comprising a nucleotide. The polymerase may incorporate the nucleotide into the complementary strand based on the sequence of the nucleic acid template. The polymerase and the captured nucleic acid template hybridized to the complementary strand, including the incorporated nucleotide, may be contacted with a second fluid comprising a magnetic particle. The incorporated nucleotide may capture the magnetic particle from the second fluid. The captured magnetic particle may cause a change in electrical resistance at the magnetically-responsive sensor. The change in electrical resistance may be used to identify the incorporated nucleotide.

公开(公告)号：US20180237850A1

公开(公告)日：2018-08-23

申请号：US15752192

申请日：2016-08-12

Applicant: Illumina, Inc.

Inventor： Jeffrey G. Mandell ,  Lisa Kwok

IPC: C12Q1/6874 ,  G01N33/543 ,  G01R33/09 ,  G01R33/12 ,  B01L3/00 ,  G01N27/327

CPC classification number: C12Q1/6874 ,  B01L3/502761 ,  C12Q1/6869 ,  C12Q2563/143 ,  C12Q2565/607 ,  G01N27/3276 ,  G01N33/543 ,  G01R33/093 ,  G01R33/1276

Abstract: Sequencing-by-synthesis (SBS) method is provided that includes providing a detection apparatus that includes an array of magnetically-responsive sensors. Each of the magnetically-responsive sensors is located proximate to a respective designated space to detect a magnetic property therefrom. The detection apparatus also includes a plurality of nucleic acid template strands located within corresponding designated spaces. The method also includes conducting a plurality of SBS events to grow a complementary strand by incorporating nucleotides along each template strand. At least some of the nucleotides are attached to corresponding magnetic particles having respective magnetic properties. Each of the plurality of SBS events includes detecting changes in electrical resistance at the magnetically-responsive sensors caused by the respective magnetic properties of the magnetic particles. The method also includes determining genetic characteristics of the complementary strands based on the detected changes in electrical resistance.