公开(公告)号：US20220333157A1

公开(公告)日：2022-10-20

申请号：US17663485

申请日：2022-05-16

Applicant: ILLUMINA, INC.

Inventor： Frank J. Steemers ,  Ramesh Ramji ,  Steven Norberg ,  Lena Christiansen ,  Dmitry K. Pokholok ,  Fan Zhang

IPC: C12Q1/6806 ,  A61K47/58 ,  A61K47/69 ,  C12M1/12 ,  C12N5/00 ,  C12N5/16

Abstract: Embodiments of systems, methods, and compositions provided herein relate to hollow beads encapsulating single cells. Some embodiments include performing multiple co-assays on a single cell encapsulated within a hollow bead, including nucleic acid sequencing, preparing nucleic acid libraries, determining methylation status, identifying genomic variants, or protein analysis.

公开(公告)号：US11359226B2

公开(公告)日：2022-06-14

申请号：US16384741

申请日：2019-04-15

Applicant: ILLUMINA, INC.

Inventor： Frank J. Steemers ,  Ramesh Ramji ,  Steven Norberg ,  Lena Christiansen ,  Dmitry K. Pokholok ,  Fan Zhang

IPC: C12Q1/6806 ,  A61K47/58 ,  A61K47/69 ,  C12M1/12 ,  C12N5/00 ,  C12N5/16

Abstract: Embodiments of systems, methods, and compositions provided herein relate to hollow beads encapsulating single cells. Some embodiments include performing multiple co-assays on a single cell encapsulated within a hollow bead, including nucleic acid sequencing, preparing nucleic acid libraries, determining methylation status, identifying genomic variants, or protein analysis.

公开(公告)号：US20190352591A1

公开(公告)日：2019-11-21

申请号：US16384741

申请日：2019-04-15

Applicant: ILLUMINA, INC.

Inventor： Frank J. Steemers ,  Ramesh Ramji ,  Steven Norberg ,  Lena Christiansen ,  Dmitry K. Pokholok ,  Fan Zhang

IPC: C12M1/12 ,  C12N5/16 ,  C12N5/00 ,  A61K47/58 ,  A61K47/69

Abstract: Embodiments of systems, methods, and compositions provided herein relate to hollow beads encapsulating single cells. Some embodiments include performing multiple co-assays on a single cell encapsulated within a hollow bead, including nucleic acid sequencing, preparing nucleic acid libraries, determining methylation status, identifying genomic variants, or protein analysis.

公开(公告)号：US11999945B2

公开(公告)日：2024-06-04

申请号：US17305230

申请日：2021-07-01

Applicant: Illumina, Inc.

Inventor： Steven Norberg ,  Dmitry K. Pokholok ,  Ramesh Ramji ,  Frank J. Steemers ,  Fan Zhang

IPC: C12N15/10 ,  B01L3/00 ,  C40B30/00 ,  C40B60/02 ,  C40B80/00

CPC classification number: C12N15/1006 ,  B01L3/502761 ,  C12N15/1065 ,  C40B30/00 ,  C40B60/02 ,  C40B80/00

Abstract: Systems, methods, and compositions provided herein relate to preparation of beads encapsulating biomolecules for performing sequential reactions on the biomolecules. Some embodiments include preparation of nucleic acid reactions within the bead, wherein the bead includes pores that allow diffusion of molecules into or out of the beads while retaining other molecules of interest.

公开(公告)号：US11085036B2

公开(公告)日：2021-08-10

申请号：US16663060

申请日：2019-10-24

Applicant: Illumina, Inc.

Inventor： Steven Norberg ,  Dmitry Pokholok ,  Ramesh Ramji ,  Frank J. Steemers ,  Fan Zhang

IPC: C12N15/10 ,  B01L3/00 ,  C40B30/00 ,  C40B60/02 ,  C40B80/00

Abstract: Systems, methods, and compositions provided herein relate to preparation of beads encapsulating biomolecules for performing sequential reactions on the biomolecules. Some embodiments include preparation of nucleic acid reactions within the bead, wherein the bead includes pores that allow diffusion of molecules into or out of the beads while retaining other molecules of interest.

公开(公告)号：US20190323003A1

公开(公告)日：2019-10-24

申请号：US16456763

申请日：2019-06-28

Applicant: ILLUMINA, INC.

Inventor： Ramesh Ramji ,  Frank J. Steemers ,  Lena Christiansen ,  Dmitry K. Pokholok ,  Fan Zhang

IPC: C12N15/10 ,  C12N9/12 ,  C12N9/22

Abstract: The invention relates to a system and methods for enhancing access to nuclear informational molecules, such as DNA, RNA, and proteins, by analytical biomolecules, such as transposome complexes, by treating nuclei with a nuclear permeability enhancer, and to methods of using nuclear membrane, cell membrane, and external compartmentalization approaches as contiguity preserving elements.

公开(公告)号：US20210332349A1

公开(公告)日：2021-10-28

申请号：US17305230

申请日：2021-07-01

Applicant: Illumina, Inc.

Inventor： Steven Norberg ,  Dmitry Pokholok ,  Ramesh Ramji ,  Frank Steemers ,  Fan Zhang

IPC: C12N15/10 ,  B01L3/00 ,  C40B30/00 ,  C40B60/02 ,  C40B80/00

Abstract: Systems, methods, and compositions provided herein relate to preparation of beads encapsulating biomolecules for performing sequential reactions on the biomolecules. Some embodiments include preparation of nucleic acid reactions within the bead, wherein the bead includes pores that allow diffusion of molecules into or out of the beads while retaining other molecules of interest.