公开(公告)号：US20150184121A1

公开(公告)日：2015-07-02

申请号：US14229596

申请日：2014-03-28

Applicant: INDUSTRIAL TECHNOLOGY RESEARCH INSTITUTE

Inventor： Yin-Lung HAN ,  Tai-Rong GUO ,  Jo-Shu CHANG ,  Yung-Chong LOU ,  Chieh-Lun CHENG ,  Wan-Ju YU ,  Chih-Hsi LIU

IPC: C12N1/20

CPC classification number: C12N1/20

Abstract: The disclosure provides a culturing medium for culturing a bacterium of genus Tepidimonas, including: a carbon source which is an organic acid, selected from a group consisting of acetate, lactate and butyrate; a nitrogen source selected from a group consisting of ammonium sulfate ((NH4)2SO4), ammonium nitrate (NH4NO3), ammonium chloride (NH4Cl) and urea; phosphate; magnesium chloride (MgCl2); yeast extract; and trace elements.

Abstract translation: 本公开内容提供了培养Tepidimonas属细菌的培养基，其包括：碳源，其是选自乙酸盐，乳酸盐和丁酸盐的有机酸; 选自硫酸铵（（NH 4）2 SO 4），硝酸铵（NH 4 NO 3），氯化铵（NH 4 Cl）和尿素的氮源; 磷酸盐 氯化镁（MgCl2）; 酵母抽提物; 和微量元素。

公开(公告)号：US20160186226A1

公开(公告)日：2016-06-30

申请号：US14838612

申请日：2015-08-28

Applicant: INDUSTRIAL TECHNOLOGY RESEARCH INSTITUTE

Inventor： Yin-Lung HAN ,  Jo-Shu CHANG ,  Yung-Chong LOU ,  Chieh-Lun CHENG ,  Chih-Hsi LIU

IPC: C12P21/00

CPC classification number: C12P21/00

Abstract: The disclosure provides a method for producing extracellular proteins from genus Tepidimonas, including: performing a fermentation culturing to a bacteria of genus Tepidimonas with a culturing medium to form a fermented liquid, wherein the composition of the culturing medium includes a carbon source which is an organic acid, selected from a group consisting of acetate, lactate and butyrate; a nitrogen source selected from a group consisting of (NH4)2SO4, NH4NO3, NH4Cl and urea; phosphate; carbonate; MgCl2; yeast extract; and trace elements, and wherein the gas feeding rate for the fermentation culturing is about 0-0.1 vvm; and after the fermentation culturing is completed, collecting the fermented liquid, wherein the fermented liquid contains extracellular protein secreted from the bacteria of genus Tepidimonas.

Abstract translation: 本发明提供了从Tepidimonas属产生细胞外蛋白的方法，包括：用培养基对培养培养基进行发酵培养以形成发酵液，其中培养基的组成包括作为有机物的碳源 酸，选自乙酸盐，乳酸盐和丁酸盐; 选自（NH 4）2 SO 4，NH 4 NO 3，NH 4 Cl和尿素的氮源; 磷酸盐 碳酸盐 MgCl2; 酵母抽提物; 和微量元素，其中发酵培养的气体进料速率为约0-0.1vvm; 在发酵培养完成后，收集发酵液，其中发酵液含有从Tepidimonas属细菌分泌的细胞外蛋白质。

公开(公告)号：US20140193316A1

公开(公告)日：2014-07-10

申请号：US14143268

申请日：2013-12-30

Applicant: Industrial Technology Research Institute

Inventor： Yin-Lung HAN ,  Tai-Rong GUO ,  Jo-Shu CHANG ,  Yung-Chong LOU ,  Wan-Ju YU

IPC: C22B7/00 ,  C07K14/195

CPC classification number: C22B7/006 ,  C02F1/286 ,  C02F3/34 ,  C02F5/08 ,  C02F2101/20 ,  C02F2301/106 ,  C02F2305/00 ,  C07K14/195 ,  C12P3/00 ,  C12R1/01 ,  C22B11/04 ,  C22B11/042 ,  Y02P10/238

Abstract: A method for recycling metals is provided by using extracellular proteins excreted by a specific thermophilic bacteria strain, Tepidimonas fonticaldi sp. nov., in which the extracellular proteins show excellent metal-ion binding ability, being useful in recycling rare earth metal ions and precious metal ions from geothermal fluids, boiler solutions, industrial wastewater or hard water.

Abstract translation: 通过使用由特异性嗜热细菌菌株Tepidimonas fonticaldi sp。分泌的细胞外蛋白质来提供回收金属的方法。 其中细胞外蛋白质显示出优异的金属离子结合能力，可用于从地热液体，锅炉溶液，工业废水或硬水中回收稀土金属离子和贵金属离子。