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1.发明申请OMEGA-TRANSAMINASE MUTANTS WITH ACTIVITY IMPROVEMENTS TOWARD KETONES AND METHODS FOR PRODUCING OPTICALLY PURE AMINES 审中-公开具有活性改善的欧米茄 - 透明质酸酯，用于生产光固化胺的方法和方法公开(公告)号：US20160298092A1
公开(公告)日：2016-10-13
申请号：US15094625
申请日：2016-04-08
发明人： Jong-Shik SHIN , Eul-Soo Park , Sang-woo Han
CPC分类号： C12N9/1096 , C12P13/001 , C12Y206/01018
摘要： Provided is an omega-transaminase mutant with improved activity using a point mutation in an active site of an omega-transaminase. Specifically, provided is a method for improving activity and extending a substrate spectrum of omega-transaminase by introducing a point mutation into a wild-type omega-transaminase rendered by replacing tryptophan at position 58 with the other amino acid.
摘要翻译： 提供了一种ω-转氨酶突变体，其在ω-转氨酶的活性位点中使用点突变来改善活性。 具体地，提供了通过将位点突变引入通过用另一个氨基酸替换58位的色氨酸而得到的野生型ω-转氨酶来提高活性并延长ω-转氨酶的底物光谱的方法。
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2.发明授权Method for preparing optically active amino acid using cosubstrate shuttling of transaminase 有权使用转氨酶的辅助穿梭制备光学活性氨基酸的方法公开(公告)号：US09376694B2
公开(公告)日：2016-06-28
申请号：US14347174
申请日：2013-07-08
发明人： Jong-Shik Shin , Eul-Soo Park
CPC分类号： C12P13/14 , C12N9/1096 , C12P13/04 , C12P13/06 , C12P13/08 , C12P13/12 , C12P13/20 , C12P13/222 , C12Y206/01018 , C12Y206/01042 , Y02P20/52
摘要： The present disclosure relates to a method for preparing an optically active amino acid using cosubstrate shuttling of transaminase. The method includes coupling a reaction of converting a keto acid to an amino acid by α-transaminase and a reaction of transferring an amino group of an amine substrate by ω-transaminase (TA) using an amino acid cosubstrate. The present disclosure allows production of various optically active amino acids with high purity and high efficiency by solving the low equilibrium constant problem of transaminase and is applicable to production of various optically active amino acids in industrial scale. Since the present disclosure allows easy production of various unnatural amino acids having high reactivity and stability, which are used as pharmaceutical precursors, it can be usefully employed in preparation of pharmaceuticals, food additives and various animal feeds.
摘要翻译： 本公开内容涉及使用转氨酶的辅助穿梭制备光学活性氨基酸的方法。 该方法包括通过α-转氨酶将酮酸转化为氨基酸的反应和使用氨基酸共底物通过ω-转氨酶（TA）转移胺底物的氨基的反应。 本公开允许通过解决转氨酶的低平衡常数问题来生产具有高纯度和高效率的各种光学活性氨基酸，并且适用于工业规模生产各种光学活性氨基酸。 由于本公开允许容易地生产用作药物前体的具有高反应性和稳定性的各种非天然氨基酸，因此可以有用地用于制备药物，食品添加剂和各种动物饲料。
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公开(公告)号：US09777301B2
公开(公告)日：2017-10-03
申请号：US14352269
申请日：2013-12-13
发明人： Jong-Shik Shin , Eul-Soo Park , M. Shaheer Malik
CPC分类号： C12P13/001 , C12P13/06 , C12P41/007
摘要： Disclosed are methods for producing optically active amino acids and amines. According to the methods, α-keto acids are generated as reaction intermediates, and as a result, ω-transaminase-catalyzed kinetic resolution of racemic amino acids or amines as racemic amine compounds enables the production of optically active amine compounds without the need to use expensive α-keto acids as starting materials. Therefore, the optically active amine compounds are produced at greatly reduced costs. In addition, the optically active amine compounds have high enantiomeric excess.
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公开(公告)号：US10308916B2
公开(公告)日：2019-06-04
申请号：US15094625
申请日：2016-04-08
发明人： Jong-Shik Shin , Eul-Soo Park , Sang-woo Han
摘要： Provided is an omega-transaminase mutant with improved activity using a point mutation in an active site of an omega-transaminase. Specifically, provided is a method for improving activity and extending a substrate spectrum of omega-transaminase by introducing a point mutation into a wild-type omega-transaminase rendered by replacing tryptophan at position 58 with the other amino acid.
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5.发明申请METHOD FOR PREPARING OPTICALLY ACTIVE AMINO ACID USING COSUBSTRATE SHUTTLING OF TRANSAMINASE 有权使用透明质酸酯基质切割制备光学活性氨基酸的方法公开(公告)号：US20150284750A1
公开(公告)日：2015-10-08
申请号：US14347174
申请日：2013-07-22
发明人： Jong-Shik Shin , Eul-Soo Park
CPC分类号： C12P13/14 , C12N9/1096 , C12P13/04 , C12P13/06 , C12P13/08 , C12P13/12 , C12P13/20 , C12P13/222 , C12Y206/01018 , C12Y206/01042 , Y02P20/52
摘要： The present disclosure relates to a method for preparing an optically active amino acid using cosubstrate shuttling of transaminase. The method includes coupling a reaction of converting a keto acid to an amino acid by α-transaminase and a reaction of transferring an amino group of an amine substrate by ω-transaminase (TA) using an amino acid cosubstrate. The present disclosure allows production of various optically active amino acids with high purity and high efficiency by solving the low equilibrium constant problem of transaminase and is applicable to production of various optically active amino acids in industrial scale. Since the present disclosure allows easy production of various unnatural amino acids having high reactivity and stability, which are used as pharmaceutical precursors, it can be usefully employed in preparation of pharmaceuticals, food additives and various animal feeds.
摘要翻译： 本公开内容涉及使用转氨酶的辅助穿梭制备光学活性氨基酸的方法。 该方法包括通过α-转氨酶将酮酸转化为氨基酸的反应和使用氨基酸共底物通过ω-转氨酶（TA）转移胺底物的氨基的反应。 本公开允许通过解决转氨酶的低平衡常数问题来生产具有高纯度和高效率的各种光学活性氨基酸，并且适用于工业规模生产各种光学活性氨基酸。 由于本公开允许容易地生产用作药物前体的具有高反应性和稳定性的各种非天然氨基酸，因此可以有用地用于制备药物，食品添加剂和各种动物饲料。
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