公开(公告)号：US20210380972A1

公开(公告)日：2021-12-09

申请号：US17344030

申请日：2021-06-10

申请人： Illumina, Inc. ,  Illumina Cambridge Limited ,  Oregon Health and Science University

发明人： Andrew C. Adey ,  Ryan Mulqueen ,  Frank Steemers ,  Dmitry K Pokholok ,  Fan Zhang ,  Esther Musgrave-Brown

IPC分类号： C12N15/10 ,  C12Q1/6869 ,  C12Q1/6855

摘要： The present disclosure is concerned with compositions, methods, and kits for preparing a sequencing library. In one embodiment, methods include producing a library of target nucleic acids having the same adapter at each end and then switching the identity of one adapter to result in target nucleic acids flanked by distinct adapters.

公开(公告)号：US20180355348A1

公开(公告)日：2018-12-13

申请号：US16000505

申请日：2018-06-05

申请人： OREGON HEALTH & SCIENCE UNIVERSITY ,  ILLUMINA, INC.

发明人： Andrew C. Adey ,  Ryan Mulqueen ,  Frank J. Steemers ,  Dmitry K. Pokholok ,  Steven Norberg

IPC分类号： C12N15/10

CPC分类号： C12N15/1065 ,  C12N15/1093 ,  C12Q1/6806 ,  C12Q1/6874 ,  C12Q2523/125 ,  C40B50/18 ,  C12Q2523/101 ,  C12Q2525/191 ,  C12Q2535/122 ,  C12Q2537/143 ,  C12Q2537/159 ,  C12Q2563/159 ,  C12Q2563/179

摘要： Provided herein are methods for preparing sequencing libraries for determining the methylation status of nucleic acids from a plurality of single cells. The present methods combine split-and-pool combinatorial indexing and bisulfite treatment techniques to characterize the methylation profiles of large numbers of single cells quickly, accurately and inexpensively.