公开(公告)号：US11535883B2

公开(公告)日：2022-12-27

申请号：US15656947

申请日：2017-07-21

申请人： Illumina, Inc. ,  Oregon Health & Science University

发明人： Andrew C. Adey ,  Sarah A. Vitak ,  Frank J. Steemers

IPC分类号： C40B50/00 ,  C12Q1/6806 ,  C12Q1/6874 ,  C12N15/10 ,  C12Q1/6869

摘要： Provided herein are methods for preparing a sequencing library that includes nucleic acids from a plurality of single cells. In one embodiment, the sequencing library includes whole genome nucleic acids from the plurality of single cells. In one embodiment, the method includes generating nucleosome-depleted nuclei by chemical treatment while maintaining integrity of the nuclei. Also provided herein are compositions, such as compositions that include chemically treated nucleosome-depleted isolated nuclei.

公开(公告)号：US20230323426A1

公开(公告)日：2023-10-12

申请号：US18074790

申请日：2022-12-05

申请人： Illumina Inc. ,  Oregon Health & Science University

发明人： Andrew C. Adey ,  Sarah A. Vitak ,  Frank J. Steemers

IPC分类号： C12Q1/6806 ,  C12N15/10 ,  C12Q1/6869 ,  C12Q1/6874

CPC分类号： C12Q1/6806 ,  C12N15/1093 ,  C12N15/1065 ,  C12Q1/6869 ,  C12Q1/6874

摘要： Provided herein are methods for preparing a sequencing library that includes nucleic acids from a plurality of single cells. In one embodiment, the sequencing library includes whole genome nucleic acids from the plurality of single cells. In one embodiment, the method includes generating nucleosome-depleted nuclei by chemical treatment while maintaining integrity of the nuclei. Also provided herein are compositions, such as compositions that include chemically treated nucleosome-depleted isolated nuclei.

公开(公告)号：US20180355348A1

公开(公告)日：2018-12-13

申请号：US16000505

申请日：2018-06-05

申请人： OREGON HEALTH & SCIENCE UNIVERSITY ,  ILLUMINA, INC.

发明人： Andrew C. Adey ,  Ryan Mulqueen ,  Frank J. Steemers ,  Dmitry K. Pokholok ,  Steven Norberg

IPC分类号： C12N15/10

CPC分类号： C12N15/1065 ,  C12N15/1093 ,  C12Q1/6806 ,  C12Q1/6874 ,  C12Q2523/125 ,  C40B50/18 ,  C12Q2523/101 ,  C12Q2525/191 ,  C12Q2535/122 ,  C12Q2537/143 ,  C12Q2537/159 ,  C12Q2563/159 ,  C12Q2563/179

摘要： Provided herein are methods for preparing sequencing libraries for determining the methylation status of nucleic acids from a plurality of single cells. The present methods combine split-and-pool combinatorial indexing and bisulfite treatment techniques to characterize the methylation profiles of large numbers of single cells quickly, accurately and inexpensively.

公开(公告)号：US20180023119A1

公开(公告)日：2018-01-25

申请号：US15656947

申请日：2017-07-21

申请人： Illumina, Inc. ,  Oregon Health & Science University

发明人： Andrew C. Adey ,  Sarah A. Vitak ,  Frank J. Steemers

IPC分类号： C12Q1/68

摘要： Provided herein are methods for preparing a sequencing library that includes nucleic acids from a plurality of single cells. In one embodiment, the sequencing library includes whole genome nucleic acids from the plurality of single cells. In one embodiment, the method includes generating nucleosome-depleted nuclei by chemical treatment while maintaining integrity of the nuclei. Also provided herein are compositions, such as compositions that include chemically treated nucleosome-depleted isolated nuclei.

公开(公告)号：US20210380972A1

公开(公告)日：2021-12-09

申请号：US17344030

申请日：2021-06-10

申请人： Illumina, Inc. ,  Illumina Cambridge Limited ,  Oregon Health and Science University

发明人： Andrew C. Adey ,  Ryan Mulqueen ,  Frank Steemers ,  Dmitry K Pokholok ,  Fan Zhang ,  Esther Musgrave-Brown

IPC分类号： C12N15/10 ,  C12Q1/6869 ,  C12Q1/6855

摘要： The present disclosure is concerned with compositions, methods, and kits for preparing a sequencing library. In one embodiment, methods include producing a library of target nucleic acids having the same adapter at each end and then switching the identity of one adapter to result in target nucleic acids flanked by distinct adapters.