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公开(公告)号：US20230383342A1
公开(公告)日：2023-11-30
申请号：US18325610
申请日：2023-05-30
申请人： Illumina Cambridge Limited , Illumina Singapore Pte. Ltd. , Illumina, Inc. , Illumina Software, Inc.
发明人： Xiaolin Wu , Patrick McCauley , Madushani Dharmarwardana , Saurabh Nirantar , Misha Golynskiy , Xiangyuan Yang , Ramesh Neelakandan , Benedict Mackworth , Carole Anastasi , Aathavan Karunakaran , Gery M. Vessere , David Bracher
IPC分类号： C12Q1/6869 , G01N21/64
CPC分类号： C12Q1/6869 , G01N2021/6439 , G01N2021/6419 , G01N21/6428
摘要： Embodiments of the present disclosure relate to kits, compositions, and methods for nucleic acid sequencing, for example, two-channel nucleic acid sequencing by synthesis using blue and green light excitation. In particular, unlabeled nucleotides for incorporation may be used in conjunction with affinity reagents containing detectable labels excitable by blue and/or green lights, for specific binding to each type of nucleotides incorporated.
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公开(公告)号：US20230357307A1
公开(公告)日：2023-11-09
申请号：US18311876
申请日：2023-05-03
发明人： Abdul Sadeer Abd Salam , Xiangyuan Yang , Hassan Zakiruddin Bohra , Yin Nah Teo , Min Yen Lee , Ramesh Neelakandan , Jeffrey G. Mandell , Erin Garcia , Sharyuen Soh , Daniel Hartoyo Lukamto
IPC分类号： C07H21/00 , C12Q1/6869 , C07H19/10
CPC分类号： C07H21/00 , C12Q1/6869 , C07H19/10
摘要： In one aspect, the disclosed technology relates to nanopore sequencing with a polynucleotide comprising a plurality of nucleotides, wherein each nucleotide comprises a linker construct between two positions of the nucleotide, wherein the linker construct optionally comprises a reporter moiety corresponding to the identity of the nucleotide, and wherein the linker construct is a part of the cleavable cyclic loop nucleotide comprising a cleavable site. In some embodiments, the nucleotides further comprise arresting constructs for slowing or halting the polynucleotide translocation through a nanopore.
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公开(公告)号：US20230332197A1
公开(公告)日：2023-10-19
申请号：US18192291
申请日：2023-03-29
发明人： Ramesh Neelakandan , Daniel Hartoyo Lukamto , Jean-Alexandre Richard , Yin Nah Teo , Xiangyuan Yang , Sadeer Salam , Jing Liang , Saurabh Nirantar
摘要： Embodiments of the present disclosure relate to nucleotide and nucleoside molecules with an optionally substituted 3′vinyl blocking group. Also provided herein are methods to prepare such nucleotide and nucleoside molecules. Additionally, the present disclosure provides methods of using such blocked nucleosides and nucleotides in oligonucleotide synthesis and sequencing.
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公开(公告)号：US20240240217A1
公开(公告)日：2024-07-18
申请号：US18530865
申请日：2023-12-06
申请人： Illumina, Inc.
IPC分类号： C12P19/34 , C07H15/26 , C07H19/048 , C12Q1/6869 , G01N21/64
CPC分类号： C12P19/34 , C07H15/26 , C07H19/048 , C12Q1/6869 , G01N21/6428 , G01N2021/6439
摘要： Embodiments of the present disclosure relate to nucleotide and nucleoside molecules with 3′ vinyl or isonitrile containing blocking groups and/or tetrazine or strained unsaturated ring containing cleavable linkers. Additionally, the present disclosure provides methods of using the nucleoside/nucleotide in oligonucleotide synthesis, and methods of sequencing using the nucleotide described herein.
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5.发明公开公开(公告)号：US20240317793A1
公开(公告)日：2024-09-26
申请号：US18608556
申请日：2024-03-18
申请人： Illumina, Inc.
发明人： Hassan Bohra , Xiangyuan Yang , Ramesh Neelakandan , Yin Nah Teo , Abdul Sadeer Abd Salam , Eric Murtfeldt
IPC分类号： C07H21/00
CPC分类号： C07H21/00
摘要： In some examples, a nucleoside triphosphate analogue may include a sugar, a nucleobase coupled to the sugar, a triphosphate group coupled to the sugar, a heteroatom coupled to an alpha phosphate of the triphosphate group, and a first substituent coupled to the heteroatom. The heteroatom may be selected from the group consisting of oxygen, nitrogen, and carbon. The first substituent may include at least one of an alkyl chain or a polymer.
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