公开(公告)号：US08956871B2

公开(公告)日：2015-02-17

申请号：US13713327

申请日：2012-12-13

Applicant: Industrial Technology Research Institute

Inventor： Pei-Ju Lin ,  Guilhem Tourniaire ,  Yi-Chen Chen ,  Kathryn Swindells ,  Bin-Ru She ,  Hsiang-Chun Hsu ,  Chih-Ching Liao ,  Su-Yo Lin

IPC: C12N5/00 ,  C12N5/0775 ,  C12N5/07 ,  C08F220/14 ,  C08F220/18 ,  C08F220/28 ,  C08F222/26 ,  C08F220/06

CPC classification number: C12N5/0031 ,  C08F220/06 ,  C08F220/14 ,  C08F220/18 ,  C08F220/28 ,  C08F222/26 ,  C12N5/0037 ,  C12N5/0068 ,  C12N2533/30

Abstract: The disclosure provides a cell culture system and a serum-free method for cultivating cells. The cell culture system includes a substratum, wherein the substratum has a surface. A polymer is disposed on the surface of the substratum, wherein the polymer is prepared by polymerizing a first monomer with a second monomer. The first monomer has a structure as represented by Formula (I), and the second monomer has a structure as represented by Formula (II): wherein, R1 is hydrogen or methyl; R2 is methyl, ethyl, or —CH2CH2OCH3; R3 is hydrogen or methyl; and, R4 is hydrogen, —CH2CH2OCOCHCHCOOH, —CH2CH2OCOCH2CH2COOH, or —CH2CH2COOH.

Abstract translation: 本公开提供了一种培养细胞的细胞培养系统和无血清方法。 细胞培养系统包括底物，其中底物具有表面。 聚合物设置在基底的表面上，其中聚合物通过使第一单体与第二单体聚合来制备。 第一单体具有由式（I）表示的结构，第二单体具有由式（II）表示的结构：其中，R 1为氢或甲基; R2是甲基，乙基或-CH2CH2OCH3; R3是氢或甲基; 和R 4是氢，-CH 2 CH 2 OCOCHCHCOOH，-CH 2 CH 2 OCOCH 2 CH 2 COOH或-CH 2 CH 2 COOH。