公开(公告)号：US20180163184A1

公开(公告)日：2018-06-14

申请号：US15838361

申请日：2017-12-12

Applicant: Industrial Technology Research Institute

Inventor： Yi-Chau Huang ,  Huai-Lo Lee ,  Yu-Yin Tsai ,  Chih-Lung Lin

IPC: C12N9/06 ,  C12Q1/26

Abstract: The fructosyl amino acid oxidase (FPOX-CE) of other species, wild-type fructosyl amino acid oxidase (PnFPOX), and the recombinant proteins (PnFPOX-SS, PnFPOX-DS, and PnFPOX-TS) were respectively dissolved in a 100 mM potassium phosphate buffer (pH value of 8.0) to achieve a concentration of 0.05 mg/ml, and after a heat treatment at 25° C. to 60° C. for 10 minutes, the oxidase activities of the above-mentioned fructosyl amino acid oxidases and recombinant proteins were measured. At the same time, thermal stability (%) was calculated based on the following formula: thermal stability=(value of oxidase activity after heat treatment)/(value of oxidase activity without heat treatment)×100%.

公开(公告)号：US10533160B2

公开(公告)日：2020-01-14

申请号：US15838361

申请日：2017-12-12

Applicant: Industrial Technology Research Institute

Inventor： Yi-Chau Huang ,  Huai-Lo Lee ,  Yu-Yin Tsai ,  Chih-Lung Lin

IPC: C12N9/02 ,  C12N9/06 ,  C12Q1/26 ,  G01N33/72

Abstract: A recombinant protein and a preparation method and application thereof are provided. The recombinant protein includes a peptide, and the peptide includes an amino acid sequence after substitution of the sequence of SEQ ID NO: 1. The substitution includes at least one selected from the group consisting of the following substitutions: the amino acid at position 182 of SEQ ID NO: 1 is substituted with aspartic acid (Asp); the amino acid at position 268 of SEQ ID NO: 1 is substituted with aspartic acid (Asp); and the amino acid at position 384 of SEQ ID NO: 1 is substituted with tyrosine (Tyr). The recombinant protein is suitable for detecting glycated hemoglobin.

公开(公告)号：US08703070B1

公开(公告)日：2014-04-22

申请号：US13667341

申请日：2012-11-02

Applicant: Industrial Technology Research Institute

Inventor： Shaw-Hwa Parng ,  Chih-Wen Yang ,  Yu-Yin Tsai ,  Yi-Chau Huang

IPC: B01L3/00 ,  G01N21/64

CPC classification number: B01L3/5025 ,  B01L3/5027 ,  B01L3/502738 ,  B01L3/502761 ,  B01L2200/0668 ,  B01L2300/0672 ,  B01L2300/0803 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/0877 ,  B01L2300/123 ,  B01L2400/0406 ,  B01L2400/0409 ,  B01L2400/043 ,  B01L2400/0683 ,  G01N21/6452 ,  G01N35/00069 ,  G01N2035/0439 ,  G01N2035/0449 ,  Y10S435/808 ,  Y10S436/805 ,  Y10S436/809 ,  Y10T436/117497 ,  Y10T436/118339

Abstract: Apparatus for immunoassay includes: a cartridge, including at least one test unit; a pin-film assembly, having a second sealing film, a plurality of pierce mechanisms, and a first actuation unit; a plurality of magnetic particles; at least one first magnetic unit; and at least one second magnetic unit. The test unit includes a plurality of fluid chambers, a plurality of pin chambers, a microchannel structure, a buffer chamber, a detection chamber and a waste chamber. The first actuation unit drives the pierce mechanisms to enable a working fluid to flow into the detection chamber storing the magnetic particles. As the second magnetic unit has a magnetic force larger than that of the first magnetic unit and can move reciprocatingly between a third position and a fourth position, the magnetic particles are driven to move reciprocatingly inside the detection chamber, thereby fully mixing the magnetic particles with the working fluid.

Abstract translation: 用于免疫测定的装置包括：盒，包括至少一个测试单元; 销片组件，具有第二密封膜，多个穿孔机构和第一致动单元; 多个磁性粒子; 至少一个第一磁性单元; 和至少一个第二磁性单元。 测试单元包括多个流体室，多个销腔，微通道结构，缓冲室，检测室和废物室。 第一致动单元驱动穿孔机构以使工作流体流入存储磁性颗粒的检测室。 由于第二磁性单元的磁力大于第一磁性单元的磁力，并且能够在第三位置与第四位置之间往复运动，驱动磁性颗粒在检测室内往复运动，从而将磁性颗粒与 工作流体。