公开(公告)号：US20220205968A1

公开(公告)日：2022-06-30

申请号：US17605823

申请日：2020-04-27

申请人： Institute for Environmental Health, Inc.

发明人： Mansour Samadpour

IPC分类号： G01N33/02

摘要： Provided are methods for rapid detection of microbial contaminants in fresh foods, comprising obtaining a product sample (preferably at or near the time of harvest of the product), applying, prior to any chilling of the sample or the product, an amount of a enrichment medium sufficient to cover the sample surfaces to provide a non-chilled sample for incubation, incubating the non-chilled sample for a time period and at a temperature sufficient to allow for amplification and enrichment of a non-cold-shocked target microbe, followed by recovering all or a portion of the enrichment media, concentrating the target microbes from the recovered enrichment medium, and detecting the concentrated target microbes.

公开(公告)号：US20150125853A1

公开(公告)日：2015-05-07

申请号：US14597733

申请日：2015-01-15

申请人： Institute for Environmental Health, Inc.

发明人： Mansour Samadpour

IPC分类号： C12Q1/68 ,  C12Q1/70

摘要： Aspects of the present invention provide novel multi-targeted microbiological screening and monitoring methods having substantial utility for monitoring and control of microbial growth and contaminants, microbiological processes, predictive microbiology, and for exposure and risk assessment. Microbial markers shared by both target and index microbes are used in novel methods for microbial monitoring, monitoring of microbial performance potential, trend analysis, and statistical process control (SPC) in processes or systems that are receptive to a plurality of genetically distinct microbes.

摘要翻译： 本发明的方面提供新颖的多靶点微生物筛选和监测方法，其具有用于监测和控制微生物生长和污染物，微生物过程，预测微生物学以及暴露和风险评估的实用性。 用于微生物监测，微生物性能潜力监测，趋势分析和统计过程控制（SPC）的新方法均可用于接受多种基因不同微生物的过程或系统中。

公开(公告)号：US20130177920A1

公开(公告)日：2013-07-11

申请号：US13784606

申请日：2013-03-04

申请人： INSTITUTE FOR ENVIRONMENTAL HEALTH, INC

发明人： Mansour Samadpour

IPC分类号： G01N1/28

CPC分类号： C12Q1/10 ,  C12Q1/04 ,  C12Q1/18 ,  C12Q1/24 ,  G01N1/28 ,  Y10S435/849

摘要： Particular aspects provide a method of sampling, testing and validating test lots (e.g., single-unit production lots), comprising: assembling a plurality of product portions from each of a plurality of test lots and combining the collected product portions to provide a corresponding set of test lot samples (wherein each test lot sample is attributed to a particular corresponding test lot); enriching the set of test lot samples; removing equal portions of each enriched sample, and combining the removed portions to provide a modular composite sample; and testing of the modular composite sample for the target agent/organism, wherein where such testing is positive, individual test lots may nonetheless yet be validated by further testing of a respective enriched test lot sample and obtaining a negative test result. The methods have broad utility for monitoring all sorts of test lots (e.g., environmental lots, production lots, pharmaceutical lots, etc.) and for efficiently affecting informed, targeted remedial measures.

摘要翻译： 特定方面提供了一种采样，测试和验证测试批（例如单一单位生产批）的方法，包括：从多个测试批中的每一个组装多个产品部分并组合收集的产品部分以提供相应的组 的测试批次样品（其中每个测试批样品归因于特定的相应测试批次）; 丰富了一批试样批次; 除去每个富集的样品的相等部分，并组合去除的部分以提供模块化的复合样品; 以及用于目标试剂/生物的模块化复合材料样品的测试，其中在这种测试为阳性的情况下，单个测试批可以通过进一步测试相应的富集测试批样品并获得阴性测试结果来验证。 这些方法具有广泛的用途，用于监测各种测试批（例如环境批次，生产批次，药品批次等），并有效地影响有针对性的有针对性的补救措施。

公开(公告)号：US20240241144A1

公开(公告)日：2024-07-18

申请号：US18414388

申请日：2024-01-16

申请人： Institute for Environmental Health, Inc.

发明人： Mansour Samadpour

IPC分类号： G01N35/02 ,  C12M1/12 ,  C12M1/34 ,  C12M1/36 ,  G01N35/04 ,  G01N35/10

CPC分类号： G01N35/025 ,  C12M25/04 ,  C12M41/46 ,  C12M41/48 ,  G01N35/1065 ,  G01N2035/0429 ,  G01N2035/0441

摘要： Provided are integrated automated systems for quantitative and/or qualitative microbiological assessment and/or analyte assessment of samples using in situ generated culture devices. The systems comprise: an integrated automated system comprising sterile media and/or buffer reagents, culture device parts, and automated components for media and/or buffer handling, test sample handling, culture device assembly, and microbial enumeration and/or detection, all within a sterile environment; and one or more processors; and memory, including computer-executable instructions that, if executed by the one or more processors, cause the integrated automated system to determine that a coded test sample, loaded into the system, is to be quantitatively and/or qualitatively assessed, and accordingly process and microbiologically assess the coded test sample using, for quantitative assessment, one or more specified in situ generated culture devices for quantitative assessment, thereby eliminating the need to sterilize, ship, store, and/or open ex situ fabricated culture devices prior to inoculation thereof.

公开(公告)号：US20230089107A1

公开(公告)日：2023-03-23

申请号：US17802094

申请日：2021-02-19

申请人： Institute for Environmental Health, Inc.

发明人： Mansour Samadpour

IPC分类号： C12M1/00

摘要： Provided are sealable transport incubators for uniform incubation of sample specimens (e.g., microbial samples) during transport between two locations (e.g., microbial sampling and detection locations, the incubators comprising a multi-slot thermal-transfer divider each in uniform thermal communication with a heat source (e.g., electrical or phase change materials (PCM), and configured to provide for uniform heat transfer and distribution to the sample specimen during transport in the transport incubator. Additionally provided are methods for expediting provision of microbial assay detection results (e.g., using the disclosed sealable transport incubators).

公开(公告)号：US10752959B2

公开(公告)日：2020-08-25

申请号：US14597733

申请日：2015-01-15

申请人： Institute for Environmental Health, Inc.

发明人： Mansour Samadpour

IPC分类号： C12Q1/68 ,  C12Q1/689 ,  C12Q1/04 ,  G01N33/569 ,  C12Q1/06 ,  C12Q1/70

摘要： Aspects of the present invention provide novel multi-targeted microbiological screening and monitoring methods having substantial utility for monitoring and control of microbial growth and contaminants, microbiological processes, predictive microbiology, and for exposure and risk assessment. Microbial markers shared by both target and index microbes are used in novel methods for microbial monitoring, monitoring of microbial performance potential, trend analysis, and statistical process control (SPC) in processes or systems that are receptive to a plurality of genetically distinct microbes.

公开(公告)号：US20140237895A1

公开(公告)日：2014-08-28

申请号：US14352304

申请日：2012-10-18

申请人： Institute for Environmental Health, Inc.

发明人： Mansour Samadpour

IPC分类号： A01G1/00

CPC分类号： A01G9/02 ,  A01C1/02 ,  A01G22/00

摘要： Provided are methods for growing and shipping sprouts and microgreens in the same container, growing while in shipment using moisture provided in a water-absorbent layer, with optional added beneficials, and including methods for producing sprouts and microgreens for consumption, and for pharmaceutical/nutriceutical use, comprising growth of sprouts in retail-ready containers, the container comprising a moisture-retaining layer of agar media or the like providing water for growth and obviating the need for irrigation during sprout growth. In certain aspects, media is supplemented with beneficial organisms or additives such as probiotic microbes, vitamins (e.g., B12), cofactors, nutrients, and other items (e.g., phytochemicals, natural colors, and antioxidants) which promote the growth of the beneficial microbes on the product, and/or which become incorporated into the product. In certain aspects, added beneficial microorganisms are selected to compete antagonize human pathogens such as Listeria, Salmonella, enterohaemorrhagic E. coli, Yersinia, and/or spoilage organisms (e.g., Erwinia, Pseudomonas and Xanthomonas).

摘要翻译： 提供了用于在同一容器中生长和运输发芽和微绿色的方法，在使用吸水层中提供的水分的情况下生长并且具有任选的附加益处，并且包括用于生产用于消费的芽菜和微生物的方法，以及用于药物/营养品 使用，包括在零售容器中生长芽，该容器包含提供用于生长的水的琼脂培养基等的保湿层，并且消除了在芽苗生长期间的灌溉需要。 在某些方面，培养基补充有益生物或添加剂，例如益生菌微生物，维生素（例如B12），辅因子，营养素和其它促进有益微生物生长的物质（如植物化学物质，天然色素和抗氧化剂） 和/或将其并入产品中。 在某些方面，选择添加的有益微生物以竞争拮抗人类病原体如李斯特菌，沙门氏菌，肠出血性大肠杆菌，耶尔森氏菌和/或腐败生物体（例如欧文氏菌，假单胞菌属和黄单胞菌属）。

公开(公告)号：US20240240227A1

公开(公告)日：2024-07-18

申请号：US18414396

申请日：2024-01-16

申请人： Institute for Environmental Health, Inc.

发明人： Mansour Samadpour

IPC分类号： C12Q1/22 ,  C12Q1/06

CPC分类号： C12Q1/22 ,  C12Q1/06

摘要： Provided are integrated automated systems for quantitative and/or qualitative microbiological assessment and/or analyte assessment of samples using in situ generated culture devices. The systems comprise: an integrated automated system comprising sterile media and/or buffer reagents, culture device parts, and automated components for media and/or buffer handling, test sample handling, culture device assembly, and microbial enumeration and/or detection, all within a sterile environment; and one or more processors; and memory, including computer-executable instructions that, if executed by the one or more processors, cause the integrated automated system to determine that a coded test sample, loaded into the system, is to be quantitatively and/or qualitatively assessed, and accordingly process and microbiologically assess the coded test sample using, for quantitative assessment, one or more specified in situ generated culture devices for quantitative assessment, thereby eliminating the need to sterilize, ship, store, and/or open ex situ fabricated culture devices prior to inoculation thereof.

公开(公告)号：US20210147913A1

公开(公告)日：2021-05-20

申请号：US17000209

申请日：2020-08-21

申请人： Institute for Environmental Health, Inc.

发明人： Mansour Samadpour

IPC分类号： C12Q1/689 ,  C12Q1/04 ,  G01N33/569 ,  C12Q1/06 ,  C12Q1/70

摘要： Aspects of the present invention provide novel multi-targeted microbiological screening and monitoring methods having substantial utility for monitoring and control of microbial growth and contaminants, microbiological processes, predictive microbiology, and for exposure and risk assessment. Microbial markers shared by both target and index microbes are used in novel methods for microbial monitoring, monitoring of microbial performance potential, trend analysis, and statistical process control (SPC) in processes or systems that are receptive to a plurality of genetically distinct microbes.

公开(公告)号：US20200340993A1

公开(公告)日：2020-10-29

申请号：US16846188

申请日：2020-04-10

申请人： Institute for Environmental Health, Inc.

发明人： Mansour Samadpour

IPC分类号： G01N33/569 ,  G01N33/543 ,  C12Q1/689

摘要： The invention provides methods and kits for the rapid confirmation of an initial analyte test result. In a preferred embodiment, the process confirms the presence of a given microbial target in a mixed culture, or a mixed enrichment media, even when the competing organisms in the mix belong to related species, or are various biotypes of the same species.