公开(公告)号：US11684918B2

公开(公告)日：2023-06-27

申请号：US16697845

申请日：2019-11-27

Applicant: IntegenX Inc.

Inventor： William D. Nielsen ,  Richard J. Belcinski ,  Gregory Bogdan ,  David Eberhart ,  Omar El-Sissi ,  Stevan B. Jovanovich ,  Michael Recknor ,  Ezra Van Gelder ,  David Wyrick

IPC: B01L3/00 ,  B01L7/00 ,  G01N27/447 ,  B01F33/30 ,  B01F33/452 ,  G01N35/00

CPC classification number: B01L3/502715 ,  B01F33/30 ,  B01F33/452 ,  B01L3/5029 ,  B01L3/527 ,  B01L7/52 ,  G01N27/44743 ,  G01N27/44791 ,  B01L2200/025 ,  B01L2200/028 ,  B01L2200/04 ,  B01L2200/0647 ,  B01L2200/143 ,  B01L2300/023 ,  B01L2300/027 ,  B01L2300/0672 ,  B01L2300/123 ,  B01L2400/043 ,  B01L2400/0421 ,  B01L2400/0478 ,  B01L2400/0487 ,  B01L2400/0616 ,  B01L2400/0638 ,  G01N2035/0091 ,  G01N2035/00158

Abstract: This disclosure provides an integrated and automated sample-to-answer system that, starting from a sample comprising biological material, generates a genetic profile in less than two hours. In certain embodiments, the biological material is DNA and the genetic profile involves determining alleles at one or a plurality of loci (e.g., genetic loci) of a subject, for example, an STR (short tandem repeat) profile, for example as used in the CODIS system. The system can perform several operations, including (a) extraction and isolation of nucleic acid; (b) amplification of nucleotide sequences at selected loci (e.g., genetic loci); and (c) detection and analysis of amplification product. These operations can be carried out in a system that comprises several integrated modules, including an analyte preparation module; a detection and analysis module and a control module.