公开(公告)号：US06387268B1

公开(公告)日：2002-05-14

申请号：US09619505

申请日：2000-07-19

申请人： Irving Wainer ,  Yanxiao Zhang

发明人： Irving Wainer ,  Yanxiao Zhang

IPC分类号： B01D1508

CPC分类号： B01D15/3804 ,  B01J20/28033 ,  B01J20/286 ,  B01J20/3204 ,  B01J20/3242 ,  B01J20/3246 ,  B01J20/3251 ,  B01J20/3274 ,  B01J2220/54 ,  B01J2220/58 ,  G01N30/02 ,  G01N30/48 ,  G01N30/7233 ,  G01N30/89 ,  G01N2030/527

摘要： This invention provides a immobilized receptors on supports in liquid chromatographic systems. The method of the invention provides means of evaluating the attachment of agents to receptors comprising the steps of: (a) immobilizing receptors on artificial membrane supports in a column, (b) exposing the supports with the receptors to test agents at varying concentrations in a liquid chromatographic system, (c) eluting the test agent from the column, and (d) evaluating the elution profile of the test materials from the column. Using this method, it is possible to evaluate the interaction of the test agent with the receptor. Following elution, it is possible to directly determine molecular structure by passing the elute through other testing devices such as a mass spectrometer.

摘要翻译： 本发明在液相色谱系统中提供载体上的固定化受体。 本发明的方法提供了评估试剂与受体的连接的方法，包括以下步骤：（a）将受体固定在柱上的人造膜支持物上，（b）将载体与受体暴露于不同浓度的试剂 液相色谱系统，（c）从柱中洗脱测试剂，（d）评估测试材料从柱中的洗脱曲线。使用该方法，可以评估测试试剂与受体的相互作用。 洗脱后，可以通过将洗脱液通过其它测试装置如质谱仪来直接确定分子结构。

公开(公告)号：US6139735A

公开(公告)日：2000-10-31

申请号：US255881

申请日：1999-02-23

申请人： Irving Wainer ,  Yanxiao Zhang

发明人： Irving Wainer ,  Yanxiao Zhang

IPC分类号： B01J20/281 ,  B01D15/08 ,  B01J20/28 ,  B01J20/32 ,  C12Q1/02 ,  G01N30/02 ,  G01N30/52 ,  G01N30/72 ,  G01N30/88 ,  G01N30/89

CPC分类号： B01D15/3804 ,  B01J20/28033 ,  B01J20/286 ,  B01J20/3204 ,  B01J20/3242 ,  B01J20/3246 ,  B01J20/3251 ,  B01J20/3274 ,  G01N30/48 ,  B01J2220/54 ,  B01J2220/58 ,  G01N2030/527 ,  G01N30/02 ,  G01N30/7233 ,  G01N30/89

摘要： This invention provides a immobilized receptors on supports in liquid chromatographic systems. The method of the invention provides means of evaluating the attachment of agents to receptors comprising the steps of:(a) immobilizing receptors on artificial membrane supports in a column,(b) exposing the supports with the receptors to test agents at varying concentrations in a liquid chromatographic system,(c) eluting the test agent from the column, and(d) evaluating the elution profile of the test materials from the column.Using this method, it is possible to evaluate the interaction of the test agent with the receptor. Following elution, it is possible to directly determine molecular structure by passing the elute through other testing devices such as a mass spectrometer.