公开(公告)号：US20030054378A1

公开(公告)日：2003-03-20

申请号：US10103798

申请日：2002-03-25

申请人： Isao Karube

发明人： Isao Karube ,  Shinya Sawata ,  Ryohei Nagata

IPC分类号： C12Q001/68 ,  C12P019/34

CPC分类号： C12Q1/6832 ,  C12Q1/686 ,  C12Q2531/107 ,  C12Q2565/501

摘要： An object of the present invention is to provide a method for detecting a target nucleotide sequence using a complementary nucleotide sequence that has an excellent sensitivity of detection. The method comprises the steps of converting the target nucleotide sequence to a partially double-stranded nucleotide sequence which is double-stranded at one part and single-stranded in the remaining part and detecting said partially double stranded nucleotide sequence using a nucleotide sequence that is complementary to the target nucleotide sequence.

公开(公告)号：US20030165953A1

公开(公告)日：2003-09-04

申请号：US10334831

申请日：2003-01-02

申请人： Isao KARUBE

发明人： Isao Karube ,  Shinya Sawata ,  Ryohei Nagata

IPC分类号： C12Q001/68 ,  C12P019/34 ,  C12M001/34

CPC分类号： C12Q1/6825 ,  C12Q1/6813 ,  Y02A50/52 ,  C12Q2565/627 ,  C12Q2525/107

摘要： An object of the present invention is to provide a method for detecting a target nucleotide sequence using the hybridization method, which has a remarkably improved sensitivity of detection. The method comprises the steps of hybridizing a target nucleotide sequence with a PNA that is complementary to the whole or a part of the target nucleotide sequence and measuring the degree of hybridization at the presence of a denaturing agent.

摘要翻译： 本发明的目的是提供一种使用杂交方法检测靶核苷酸序列的方法，其具有显着提高的检测灵敏度。 该方法包括以下步骤：将目标核苷酸序列与与靶核苷酸序列的全部或部分互补的PNA杂交并测定变性剂存在下的杂交程度。

公开(公告)号：US20030224441A1

公开(公告)日：2003-12-04

申请号：US10462747

申请日：2003-06-17

申请人： DAI NIPPON PRINTING CO., LTD. ,  Isao KARUBE

发明人： Runa Nakamura ,  Hiroyuki Nakamura ,  Ryohei Nagata ,  Isao Karube ,  Hitoshi Muguruma

IPC分类号： C12Q001/68 ,  G01N033/53 ,  C12M001/34 ,  C12M003/00 ,  G01J003/00

CPC分类号： G01N21/553

摘要： An objective of the present invention is to provide a measuring chip for a surface plasmon resonance sensor that can detect a small amount of target substances in high sensitivity. The present invention provides a measuring chip for a surface plasmon resonance sensor comprising a metal layer, one or more plasma polymerization layers formed on said metal layer, and a biologically active substance immobilized on the surface of said plasma polymerization layer.

摘要翻译： 本发明的目的是提供一种能够以高灵敏度检测少量目标物质的表面等离子体共振传感器的测量芯片。 本发明提供了一种用于表面等离子体共振传感器的测量芯片，其包括金属层，在所述金属层上形成的一个或多个等离子体聚合层和固定在所述等离子体聚合层的表面上的生物活性物质。