公开(公告)号：US20030054378A1

公开(公告)日：2003-03-20

申请号：US10103798

申请日：2002-03-25

申请人： Isao Karube

发明人： Isao Karube ,  Shinya Sawata ,  Ryohei Nagata

IPC分类号： C12Q001/68 ,  C12P019/34

CPC分类号： C12Q1/6832 ,  C12Q1/686 ,  C12Q2531/107 ,  C12Q2565/501

摘要： An object of the present invention is to provide a method for detecting a target nucleotide sequence using a complementary nucleotide sequence that has an excellent sensitivity of detection. The method comprises the steps of converting the target nucleotide sequence to a partially double-stranded nucleotide sequence which is double-stranded at one part and single-stranded in the remaining part and detecting said partially double stranded nucleotide sequence using a nucleotide sequence that is complementary to the target nucleotide sequence.

公开(公告)号：US20030165953A1

公开(公告)日：2003-09-04

申请号：US10334831

申请日：2003-01-02

申请人： Isao KARUBE

发明人： Isao Karube ,  Shinya Sawata ,  Ryohei Nagata

IPC分类号： C12Q001/68 ,  C12P019/34 ,  C12M001/34

CPC分类号： C12Q1/6825 ,  C12Q1/6813 ,  Y02A50/52 ,  C12Q2565/627 ,  C12Q2525/107

摘要： An object of the present invention is to provide a method for detecting a target nucleotide sequence using the hybridization method, which has a remarkably improved sensitivity of detection. The method comprises the steps of hybridizing a target nucleotide sequence with a PNA that is complementary to the whole or a part of the target nucleotide sequence and measuring the degree of hybridization at the presence of a denaturing agent.

摘要翻译： 本发明的目的是提供一种使用杂交方法检测靶核苷酸序列的方法，其具有显着提高的检测灵敏度。 该方法包括以下步骤：将目标核苷酸序列与与靶核苷酸序列的全部或部分互补的PNA杂交并测定变性剂存在下的杂交程度。