公开(公告)号：US20070181058A1

公开(公告)日：2007-08-09

申请号：US10567666

申请日：2004-08-10

申请人： Isao Tanaka ,  Nobuhisa Watanabe ,  Hiroshi Takeuchi ,  Takayuki Iseki ,  Nozomu Shibuya ,  Chiharu Nishijima

发明人： Isao Tanaka ,  Nobuhisa Watanabe ,  Hiroshi Takeuchi ,  Takayuki Iseki ,  Nozomu Shibuya ,  Chiharu Nishijima

IPC分类号： C30B15/26 ,  C30B11/00

CPC分类号： C30B7/00 ,  C07K1/306 ,  C30B29/58 ,  Y10T117/1004 ,  Y10T117/1024

摘要： The invention provides a protein crystallizing device capable of performing a protein crystallization experiment or screening of crystallization conditions rapidly and economically with a high reliability, and a protein crystallizing agent capable of performing the operation of protein crystallization by a simpler method. The protein crystallizing device of the present invention comprises a protein crystallizing microarray having at least two crystallizing agent holding parts which hold a protein crystallizing agent and a plate laminated on the protein crystallizing microarray, wherein the plate has crystallizing sections corresponding to the crystallizing agent holding parts and capable of being filled with a protein-containing sample and recessed parts provided between the crystallizing sections. The protein crystallizing agent of the present invention is evenly held in a gel by gelatinizing a solution containing a protein precipitant and an unsaturated monomer.

摘要翻译： 本发明提供能够以高可靠性快速且经济地进行蛋白质结晶实验或筛选结晶条件的蛋白质结晶装置，以及能够通过更简单的方法进行蛋白质结晶操作的蛋白质结晶剂。 本发明的蛋白质结晶装置包括蛋白结晶微阵列，其具有至少两个结晶剂保持部分，其保持蛋白质结晶剂和层压在蛋白质结晶微阵列上的板，其中该板具有对应于结晶剂保持部分的结晶部分 并且能够填充含蛋白质的样品和设置在结晶部之间的凹部。 通过将含有蛋白质沉淀剂和不饱和单体的溶液凝胶化，将本发明的蛋白质结晶剂均匀地保持在凝胶中。

公开(公告)号：US20050075482A1

公开(公告)日：2005-04-07

申请号：US10498232

申请日：2002-12-11

申请人： Isao Tanaka ,  Nobuhisa Watanabe ,  Hiroshi Takeuchi ,  Takashi Akita ,  Yuichirou Nagata ,  Toshinori Sumi ,  Chiharu Nishijima

发明人： Isao Tanaka ,  Nobuhisa Watanabe ,  Hiroshi Takeuchi ,  Takashi Akita ,  Yuichirou Nagata ,  Toshinori Sumi ,  Chiharu Nishijima

IPC分类号： B01J19/00 ,  B01L3/00 ,  B01L3/06 ,  C07B61/00 ,  C07K1/30 ,  C30B7/00 ,  C30B29/58 ,  C40B40/18 ,  C40B60/14 ,  G01N25/14 ,  B01D9/00 ,  C07K14/00 ,  C12M1/34

CPC分类号： B01L3/5085 ,  B01J19/0046 ,  B01J2219/00317 ,  B01J2219/00522 ,  B01J2219/00585 ,  B01J2219/00639 ,  B01J2219/00644 ,  B01J2219/00673 ,  B01J2219/0072 ,  B01J2219/00725 ,  B01J2219/00745 ,  B01J2219/00756 ,  B01L3/06 ,  B01L3/50853 ,  B01L2300/069 ,  B01L2300/0819 ,  B01L2300/0822 ,  C07B2200/11 ,  C07K1/306 ,  C30B7/00 ,  C30B29/58 ,  C40B40/18 ,  C40B60/14 ,  G01N25/147

摘要： The present invention relates to a method of precipitating protein crystals from a protein-containing sample. The present invention also relates to a novel microarray and a novel device for screening for protein crystallization condition. Furthermore, the present invention relates to a method of conveniently and quickly screening for protein crystallization conditions using the microarray or the device having highly integrated and held crystallization conditions even with an extremely small quantity of a sample.

摘要翻译： 本发明涉及从含蛋白质的样品中沉淀蛋白质晶体的方法。 本发明还涉及一种用于筛选蛋白质结晶条件的新型微阵列和新型装置。 此外，本发明涉及使用微阵列方法快速筛选蛋白质结晶条件的方法，即使使用非常少量的样品，也具有高度集成且保持结晶条件的装置。