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12 条记录 (耗时 0.014 秒)

    Process for the enzymatic preparation of optically-active cyanohydrins
    1.
    发明授权
    Process for the enzymatic preparation of optically-active cyanohydrins 失效
    光活性氰基化合物的制备方法

    公开(公告)号：US5122462A

    公开(公告)日：1992-06-16

    申请号：US670437

    申请日：1991-03-18

    申请人： Peter Miethe ,  Maria-Regina Kula ,  Ingeborg M. Stuertz ,  Christian Wandrey ,  Udo Kragl

    发明人： Peter Miethe ,  Maria-Regina Kula ,  Ingeborg M. Stuertz ,  Christian Wandrey ,  Udo Kragl

    IPC分类号： C12M1/00 ,  C12M1/40 ,  C12P13/00 ,  C12P41/00

    CPC分类号： C12P13/004 ,  C12M21/18 ,  C09K2219/15

    摘要： The preparation of optically-active cyanohydrins from the corresponding oxo compounds is disclosed. The reaction of the oxo compounds with hydrocyanic acid is carried out in an organic solvent in the presence of (R)- or (S)-oxynitrilase (4.1.2.10) and (4.1.2.11), respectively, being solubilized in a lyotropic liquid crystal. Compounds which upon hydrolysis produce increased pH values are excluded as surface active agents. Preferably, surface active agents, the organic solvent and an aqueous buffer solution with a pH of 3 to 6, are mixed together to obtain a liquid crystal/organic solvent two-phase system. The liquid crystal is preferably fixed on a porous support, in particular a glass support. The reaction is carried out in a flow-through reactor which contains the liquid crystal in the abovementioned form or in thin layers adjacent to narrow flow channels, the borders of which are liquid permeable and through which the substrate-containing solvent is passed.

    摘要翻译： 公开了从相应的氧代化合物制备光学活性的氰醇。 氧代化合物与氢氰酸的反应在有机溶剂中分别在（R） - 或（S） - 硝基腈酶（4.1.2.10）和（4.1.2.11）的存在下进行，溶解在溶致液体 水晶。 在水解产生增加的pH值的化合物被排除作为表面活性剂。 优选将表面活性剂，有机溶剂和pH为3〜6的缓冲水溶液混合在一起，得到液晶/有机溶剂两相体系。 液晶优选固定在多孔载体上，特别是玻璃载体上。 该反应在含有上述形式的液晶的流通反应器中或邻近窄流道的薄层中进行，该流动通道的边界是液体可渗透的并且含底物的溶剂通过该反应器。

    Method for making fluorinated sugars having a side chain and use thereof
    2.
    发明授权
    Method for making fluorinated sugars having a side chain and use thereof 失效
    制造具有侧链的氟化糖及其用途的方法

    公开(公告)号：US06355453B1

    公开(公告)日：2002-03-12

    申请号：US09483548

    申请日：2000-01-14

    申请人： Juliane Beliczey ,  Udo Kragl ,  Andreas Liese ,  Christian Wandrey ,  Kurt Hamacher ,  Heinz Hubert Coenen ,  Thomas Tierling

    发明人： Juliane Beliczey ,  Udo Kragl ,  Andreas Liese ,  Christian Wandrey ,  Kurt Hamacher ,  Heinz Hubert Coenen ,  Thomas Tierling

    IPC分类号： C12P1926

    CPC分类号： C12P19/26

    摘要： Furanosides or pyranosides can be reacted with pyruvate and converted into sialic acids in good yields by an enzymatic synthesis, using a suitable aldolase, where the concentration of aldolase, per 50 mM of pyruvate, is 1 to 2,500 U*/ml. For example, 3-fluoroneuraminic acid and other 3,3-didesoxy-3-fluorononulopyranosonic acid derivatives can be prepared in this manner. The F-atom in the resulting sialic acid (which can be 19F or 19F and 18F) provides a label which makes possible studies of mammalian physiology and diagnosis of mammalian diseases.

    摘要翻译： 呋喃糖苷或吡喃糖苷可以与丙酮酸反应，并通过酶合成，以合适的醛缩酶将其转化成唾液酸，其中醛糖醛酸的浓度为每50mM丙酮酸盐为1至2,500U / ml。 例如，可以以这种方式制备3-氟尿神经氨酸和其它的3,3-didesoxy-3-fluorononuloproranosonic acid衍生物。 所得唾液酸（其可以是19F或19F和18F）中的F原子提供了可以研究哺乳动物生理学和哺乳动物疾病诊断的标签。

    Enzymatic process for preparing N-acetylneuraminic acid
    9.
    发明授权
    Enzymatic process for preparing N-acetylneuraminic acid 失效
    用于制备N-乙酰氨基酸的酶法

    公开(公告)号：US5071750A

    公开(公告)日：1991-12-10

    申请号：US606944

    申请日：1990-10-31

    申请人： Udo Kragl ,  Christian Wandrey ,  Oreste Ghisalba ,  Daniel Gygax

    发明人： Udo Kragl ,  Christian Wandrey ,  Oreste Ghisalba ,  Daniel Gygax

    IPC分类号： C12P19/26

    CPC分类号： C12P19/26

    摘要： A process for obtaining N-acetylneuraminic acid from N-acetylglucosamine is disclosed. The process is carried out in a reactor which contains both N-acylglucosamine-2-epimerase (E.C. 5.1.3.8) which isomerizes GlcNAc into ManNAc, and N-acetylneuraminic acid pyruvate lyase (E.C. 4.1.3.3) which catalyzes the reaction of the resulting ManNAc with pyruvic acid to give Neu5Ac. GlcNAc and Pyr are fed into the reactor and Neu5Ac is obtained in the outflow. The process is preferably carried out continuously and in particular in an enzyme membrane reactor at pH 7.5 and 25.degree. C., especially using residence times of 0.2 to 10 h, and with an excess of GlcNAc in comparison with Pyr which is subsequently added if necessary. Epimerase and lyase are preferably present in the reactor in a ratio of activities which is equivalent to the reciprocal value of the quotient of the conversion rates.

    摘要翻译： 公开了从N-乙酰葡糖胺获得N-乙酰神经氨酸的方法。 该方法在含有将GlcNac异构化成ManNac的N-酰基葡糖胺-2-差向异构酶（EC 5.1.3.8）和N-乙酰神经氨酸丙酮酸裂合酶（EC 4.1.3.3）的反应器中进行，其催化所得到的 ManNac与丙酮酸反应得到Neu5Ac。 将GlcNAc和Pyr进料到反应器中，并在流出物中获得Neu5Ac。 该方法优选连续地，特别是在pH 7.5和25℃的酶膜反应器中进行，特别是使用0.2至10小时的停留时间，以及与Pyr相比过量的GlcNac，如果需要，随后加入 。 差向异构酶和裂解酶优选以相当于转化率的商的倒数值的活性比例存在于反应器中。