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公开(公告)号:US07262008B2
公开(公告)日:2007-08-28
申请号:US10215304
申请日:2002-08-08
申请人: Luigi Catanzariti , Bryan W. Kluttz , Marcela Vera-Garcia , J. Lawrence Burg , James G. Moe , Geoff A. McKinley
发明人: Luigi Catanzariti , Bryan W. Kluttz , Marcela Vera-Garcia , J. Lawrence Burg , James G. Moe , Geoff A. McKinley
CPC分类号: G01N35/026 , C12Q1/6834 , C12Q1/6846 , C12Q1/6865 , C12Q2600/166 , Y10T436/143333 , C12Q2521/301 , C12Q2565/629 , C12Q2527/125 , C12Q2525/143 , C12Q2537/125 , C12Q2565/519 , C12Q2545/101
摘要: The present invention relates to the detection of specific nucleic acid sequences, either by a process of amplification of specific nucleic acid sequences or not. More particularly the invention provides for improved compositions and methods for reducing the chance for contamination from manipulation of reagents, internal controls for amplification, and the use of automated apparatus for the automated detection of one, or more than one amplified nucleic acid sequences.
摘要翻译: 本发明涉及通过扩增特异性核酸序列的方法来检测特异性核酸序列。 更具体地,本发明提供了改进的组合物和方法,用于降低操纵试剂的污染机会,用于扩增的内部对照,以及使用用于自动检测一个或多于一个扩增的核酸序列的自动化装置。
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公开(公告)号:US06586234B1
公开(公告)日:2003-07-01
申请号:US09245940
申请日:1999-02-05
IPC分类号: C12M134
CPC分类号: G01N35/026 , C12Q1/6834 , C12Q1/6846 , C12Q2600/166 , Y10T436/143333 , C12Q2537/125 , C12Q2527/125 , C12Q2565/629 , C12Q2565/519 , C12Q2545/101
摘要: The present invention relates to the detection of specific nucleic acid sequences after an amplification process, or directly without amplification. In particular, the invention provides for the automation of the amplification and detection process, the amplification and detection of one or more specific nucleic acid sequences, the use of internal controls, reduced potential for contamination caused by the manual manipulation of reagents, and improved reagent compositions to better control assay performance and provide for further protection against contamination.
摘要翻译: 本发明涉及在扩增过程之后或直接没有扩增的情况下检测特异性核酸序列。 特别地,本发明提供了扩增和检测过程的自动化,一个或多个特定核酸序列的扩增和检测,内部对照的使用,手动操作试剂引起的污染降低的潜力和改进的试剂 组合物以更好地控制测定性能并进一步防止污染。
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公开(公告)号:US06528632B1
公开(公告)日:2003-03-04
申请号:US09586546
申请日:2000-05-31
申请人: Luigi Catanzariti , Bryan W. Kluttz , Marcela Vera-Garcia , J. Lawrence Burg , James G. Moe , Geoff A. McKinley
发明人: Luigi Catanzariti , Bryan W. Kluttz , Marcela Vera-Garcia , J. Lawrence Burg , James G. Moe , Geoff A. McKinley
IPC分类号: C07H2102
CPC分类号: G01N35/026 , C12Q1/6834 , C12Q1/6846 , C12Q2600/166 , Y10T436/143333 , C12Q2537/125 , C12Q2527/125 , C12Q2565/629 , C12Q2565/519 , C12Q2545/101
摘要: The present invention relates to the detection of specific nucleic acid sequences after an amplification process, or directly without amplification. In particular, the invention provides for the automation of the amplification and detection process, the amplification and detection of one or more specific nucleic acid sequences, the use of internal controls, reduced potential for contamination caused by the manual manipulation of reagents, and improved reagent compositions to better control assay performance and provide for further protection against contamination.
摘要翻译: 本发明涉及在扩增过程之后或直接没有扩增的情况下检测特异性核酸序列。 特别地,本发明提供了扩增和检测过程的自动化,一个或多个特定核酸序列的扩增和检测,内部对照的使用,手动操作试剂引起的污染降低的潜力和改进的试剂 组合物以更好地控制测定性能并进一步防止污染。
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公开(公告)号:US06300068B1
公开(公告)日:2001-10-09
申请号:US09245939
申请日:1999-02-05
申请人: J. Lawrence Burg , Luigi Catanzariti , Bryan W. Kluttz , Geoff A. McKinley , James G. Moe , Marcela Vera-Garcia
发明人: J. Lawrence Burg , Luigi Catanzariti , Bryan W. Kluttz , Geoff A. McKinley , James G. Moe , Marcela Vera-Garcia
IPC分类号: C12Q168
CPC分类号: G01N35/026 , C12Q1/6834 , C12Q1/6846 , C12Q2600/166 , Y10T436/143333 , C12Q2537/125 , C12Q2527/125 , C12Q2565/629 , C12Q2565/519 , C12Q2545/101
摘要: The present invention relates to the detection of specific nucleic acid sequences after an amplification process, or directly without amplification. In particular, the invention provides for the automation of the amplification and detection process, the amplification and detection of one or more specific nucleic acid sequences, the use of internal controls, reduced potential for contamination caused by the manual manipulation of reagents, and improved reagent compositions to better control assay performance and provide for further protection against contamination.
摘要翻译: 本发明涉及在扩增过程之后或直接没有扩增的情况下检测特异性核酸序列。 特别地,本发明提供了扩增和检测过程的自动化,一个或多个特定核酸序列的扩增和检测,内部对照的使用,手动操作试剂引起的污染降低的潜力和改进的试剂 组合物以更好地控制测定性能并进一步防止污染。
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公开(公告)号:US20080240985A1
公开(公告)日:2008-10-02
申请号:US11780164
申请日:2007-07-19
申请人: Luigi Catanzariti , Bryan W. Kluttz , Marcela Vera-Garcia , J. Lawrence Burg , James G. Moe , Geoff A. McKinley
发明人: Luigi Catanzariti , Bryan W. Kluttz , Marcela Vera-Garcia , J. Lawrence Burg , James G. Moe , Geoff A. McKinley
IPC分类号: B01J19/00
CPC分类号: G01N35/026 , C12Q1/6834 , C12Q1/6846 , C12Q2600/166 , Y10T436/143333 , C12Q2537/125 , C12Q2527/125 , C12Q2565/629 , C12Q2565/519 , C12Q2545/101
摘要: The present invention relates to the detection of specific nucleic acid sequences, either by a process of amplification of specific nucleic acid sequences or not. More particularly the invention provides for improved compositions and methods for reducing the chance for contamination from manipulation of reagents, internal controls for amplification, and the use of automated apparatus for the automated detection of one, or more than one amplified nucleic acid sequences.
摘要翻译: 本发明涉及通过扩增特异性核酸序列的方法来检测特异性核酸序列。 更具体地,本发明提供了改进的组合物和方法,用于降低操纵试剂的污染机会,用于扩增的内部对照,以及使用用于自动检测一个或多于一个扩增的核酸序列的自动化装置。
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公开(公告)号:US07309588B2
公开(公告)日:2007-12-18
申请号:US09901181
申请日:2001-07-09
申请人: J. Lawrence Burg , Bryan W. Kluttz , Luigi Catanzariti , Marcela Vera-Garcia , James G. Moe , Geoff A. McKinley
发明人: J. Lawrence Burg , Bryan W. Kluttz , Luigi Catanzariti , Marcela Vera-Garcia , James G. Moe , Geoff A. McKinley
CPC分类号: G01N35/026 , C12Q1/6834 , C12Q1/6846 , C12Q2600/166 , Y10T436/143333 , C12Q2537/125 , C12Q2527/125 , C12Q2565/629 , C12Q2565/519 , C12Q2545/101
摘要: The present invention relates to the detection of specific nucleic acid sequences after an amplification process, or directly without amplification. In particular, the invention provides for the automation of the amplification and detection process, the amplification and detection of one or more specific nucleic acid sequences, the use of internal controls, reduced potential for contamination caused by the manual manipulation of reagents, and improved reagent compositions to better control assay performance and provide for further protection against contamination.
摘要翻译: 本发明涉及在扩增过程之后或直接没有扩增的情况下检测特异性核酸序列。 特别地,本发明提供了扩增和检测过程的自动化,一个或多个特定核酸序列的扩增和检测,内部对照的使用,手动操作试剂引起的污染降低的潜力和改进的试剂 组合物以更好地控制测定性能并进一步防止污染。
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公开(公告)号:US06558901B1
公开(公告)日:2003-05-06
申请号:US09065914
申请日:1998-04-24
申请人: Luigi Catanzariti , Bryan W. Kluttz , Marcela Vera-Garcia , J. Lawrence Burg , James G. Moe , Geoff A. McKinley
发明人: Luigi Catanzariti , Bryan W. Kluttz , Marcela Vera-Garcia , J. Lawrence Burg , James G. Moe , Geoff A. McKinley
IPC分类号: C12Q168
CPC分类号: G01N35/026 , C12Q1/6834 , C12Q1/6846 , C12Q2600/166 , Y10T436/143333 , C12Q2537/125 , C12Q2527/125 , C12Q2565/629 , C12Q2565/519 , C12Q2545/101
摘要: The present invention relates to the detection of specific nucleic acid sequences, either by a process of amplification of specific nucleic acid sequences or not. More particularly the invention provides for improved compositions and methods for reducing the chance for contamination from manipulation of reagents, internal controls for amplification, and the use of automated apparatus for the automated detection of one, or more than one amplified nucleic acid sequences.
摘要翻译: 本发明涉及通过扩增特异性核酸序列的方法来检测特异性核酸序列。 更具体地,本发明提供了改进的组合物和方法,用于降低操纵试剂的污染机会,用于扩增的内部对照,以及使用用于自动检测一个或多于一个扩增的核酸序列的自动化装置。
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公开(公告)号:US5780273A
公开(公告)日:1998-07-14
申请号:US590804
申请日:1996-01-24
申请人: J. Lawrence Burg
发明人: J. Lawrence Burg
CPC分类号: C12N15/1093 , C12Q1/6867 , C12Q1/689
摘要: Novel insertion elements, recombinant molecules and amplifiable nucleic acids and methods for making them are disclosed. The insertion elements and amplifiable nucleic acids can be used with nucleic acid hybridization assays. Also disclosed are libraries of the novel insertion elements, recombinant molecules and amplifiable nucleic acids and the use of such libraries in identifying preferred insertion elements, recombinant molecules and amplifiable nucleic acids of the invention.
摘要翻译: 公开了新的插入元件,重组分子和可扩增核酸及其制备方法。 插入元件和可扩增核酸可以与核酸杂交测定一起使用。 还公开了新型插入元件,重组分子和可扩增核酸的文库,以及这些文库在鉴定本发明的优选插入元件,重组分子和可扩增核酸中的用途。
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