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公开(公告)号：US07262008B2
公开(公告)日：2007-08-28
申请号：US10215304
申请日：2002-08-08
申请人： Luigi Catanzariti , Bryan W. Kluttz , Marcela Vera-Garcia , J. Lawrence Burg , James G. Moe , Geoff A. McKinley
发明人： Luigi Catanzariti , Bryan W. Kluttz , Marcela Vera-Garcia , J. Lawrence Burg , James G. Moe , Geoff A. McKinley
CPC分类号： G01N35/026 , C12Q1/6834 , C12Q1/6846 , C12Q1/6865 , C12Q2600/166 , Y10T436/143333 , C12Q2521/301 , C12Q2565/629 , C12Q2527/125 , C12Q2525/143 , C12Q2537/125 , C12Q2565/519 , C12Q2545/101
摘要： The present invention relates to the detection of specific nucleic acid sequences, either by a process of amplification of specific nucleic acid sequences or not. More particularly the invention provides for improved compositions and methods for reducing the chance for contamination from manipulation of reagents, internal controls for amplification, and the use of automated apparatus for the automated detection of one, or more than one amplified nucleic acid sequences.
摘要翻译： 本发明涉及通过扩增特异性核酸序列的方法来检测特异性核酸序列。 更具体地，本发明提供了改进的组合物和方法，用于降低操纵试剂的污染机会，用于扩增的内部对照，以及使用用于自动检测一个或多于一个扩增的核酸序列的自动化装置。
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公开(公告)号：US06528632B1
公开(公告)日：2003-03-04
申请号：US09586546
申请日：2000-05-31
申请人： Luigi Catanzariti , Bryan W. Kluttz , Marcela Vera-Garcia , J. Lawrence Burg , James G. Moe , Geoff A. McKinley
发明人： Luigi Catanzariti , Bryan W. Kluttz , Marcela Vera-Garcia , J. Lawrence Burg , James G. Moe , Geoff A. McKinley
IPC分类号： C07H2102
CPC分类号： G01N35/026 , C12Q1/6834 , C12Q1/6846 , C12Q2600/166 , Y10T436/143333 , C12Q2537/125 , C12Q2527/125 , C12Q2565/629 , C12Q2565/519 , C12Q2545/101
摘要： The present invention relates to the detection of specific nucleic acid sequences after an amplification process, or directly without amplification. In particular, the invention provides for the automation of the amplification and detection process, the amplification and detection of one or more specific nucleic acid sequences, the use of internal controls, reduced potential for contamination caused by the manual manipulation of reagents, and improved reagent compositions to better control assay performance and provide for further protection against contamination.
摘要翻译： 本发明涉及在扩增过程之后或直接没有扩增的情况下检测特异性核酸序列。 特别地，本发明提供了扩增和检测过程的自动化，一个或多个特定核酸序列的扩增和检测，内部对照的使用，手动操作试剂引起的污染降低的潜力和改进的试剂 组合物以更好地控制测定性能并进一步防止污染。
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公开(公告)号：US06300068B1
公开(公告)日：2001-10-09
申请号：US09245939
申请日：1999-02-05
申请人： J. Lawrence Burg , Luigi Catanzariti , Bryan W. Kluttz , Geoff A. McKinley , James G. Moe , Marcela Vera-Garcia
发明人： J. Lawrence Burg , Luigi Catanzariti , Bryan W. Kluttz , Geoff A. McKinley , James G. Moe , Marcela Vera-Garcia
IPC分类号： C12Q168
CPC分类号： G01N35/026 , C12Q1/6834 , C12Q1/6846 , C12Q2600/166 , Y10T436/143333 , C12Q2537/125 , C12Q2527/125 , C12Q2565/629 , C12Q2565/519 , C12Q2545/101
摘要： The present invention relates to the detection of specific nucleic acid sequences after an amplification process, or directly without amplification. In particular, the invention provides for the automation of the amplification and detection process, the amplification and detection of one or more specific nucleic acid sequences, the use of internal controls, reduced potential for contamination caused by the manual manipulation of reagents, and improved reagent compositions to better control assay performance and provide for further protection against contamination.
摘要翻译： 本发明涉及在扩增过程之后或直接没有扩增的情况下检测特异性核酸序列。 特别地，本发明提供了扩增和检测过程的自动化，一个或多个特定核酸序列的扩增和检测，内部对照的使用，手动操作试剂引起的污染降低的潜力和改进的试剂 组合物以更好地控制测定性能并进一步防止污染。
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公开(公告)号：US20080240985A1
公开(公告)日：2008-10-02
申请号：US11780164
申请日：2007-07-19
申请人： Luigi Catanzariti , Bryan W. Kluttz , Marcela Vera-Garcia , J. Lawrence Burg , James G. Moe , Geoff A. McKinley
发明人： Luigi Catanzariti , Bryan W. Kluttz , Marcela Vera-Garcia , J. Lawrence Burg , James G. Moe , Geoff A. McKinley
IPC分类号： B01J19/00
CPC分类号： G01N35/026 , C12Q1/6834 , C12Q1/6846 , C12Q2600/166 , Y10T436/143333 , C12Q2537/125 , C12Q2527/125 , C12Q2565/629 , C12Q2565/519 , C12Q2545/101
摘要： The present invention relates to the detection of specific nucleic acid sequences, either by a process of amplification of specific nucleic acid sequences or not. More particularly the invention provides for improved compositions and methods for reducing the chance for contamination from manipulation of reagents, internal controls for amplification, and the use of automated apparatus for the automated detection of one, or more than one amplified nucleic acid sequences.
摘要翻译： 本发明涉及通过扩增特异性核酸序列的方法来检测特异性核酸序列。 更具体地，本发明提供了改进的组合物和方法，用于降低操纵试剂的污染机会，用于扩增的内部对照，以及使用用于自动检测一个或多于一个扩增的核酸序列的自动化装置。
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公开(公告)号：US07309588B2
公开(公告)日：2007-12-18
申请号：US09901181
申请日：2001-07-09
申请人： J. Lawrence Burg , Bryan W. Kluttz , Luigi Catanzariti , Marcela Vera-Garcia , James G. Moe , Geoff A. McKinley
发明人： J. Lawrence Burg , Bryan W. Kluttz , Luigi Catanzariti , Marcela Vera-Garcia , James G. Moe , Geoff A. McKinley
CPC分类号： G01N35/026 , C12Q1/6834 , C12Q1/6846 , C12Q2600/166 , Y10T436/143333 , C12Q2537/125 , C12Q2527/125 , C12Q2565/629 , C12Q2565/519 , C12Q2545/101
摘要： The present invention relates to the detection of specific nucleic acid sequences after an amplification process, or directly without amplification. In particular, the invention provides for the automation of the amplification and detection process, the amplification and detection of one or more specific nucleic acid sequences, the use of internal controls, reduced potential for contamination caused by the manual manipulation of reagents, and improved reagent compositions to better control assay performance and provide for further protection against contamination.
摘要翻译： 本发明涉及在扩增过程之后或直接没有扩增的情况下检测特异性核酸序列。 特别地，本发明提供了扩增和检测过程的自动化，一个或多个特定核酸序列的扩增和检测，内部对照的使用，手动操作试剂引起的污染降低的潜力和改进的试剂 组合物以更好地控制测定性能并进一步防止污染。
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公开(公告)号：US06558901B1
公开(公告)日：2003-05-06
申请号：US09065914
申请日：1998-04-24
申请人： Luigi Catanzariti , Bryan W. Kluttz , Marcela Vera-Garcia , J. Lawrence Burg , James G. Moe , Geoff A. McKinley
发明人： Luigi Catanzariti , Bryan W. Kluttz , Marcela Vera-Garcia , J. Lawrence Burg , James G. Moe , Geoff A. McKinley
IPC分类号： C12Q168
CPC分类号： G01N35/026 , C12Q1/6834 , C12Q1/6846 , C12Q2600/166 , Y10T436/143333 , C12Q2537/125 , C12Q2527/125 , C12Q2565/629 , C12Q2565/519 , C12Q2545/101
摘要： The present invention relates to the detection of specific nucleic acid sequences, either by a process of amplification of specific nucleic acid sequences or not. More particularly the invention provides for improved compositions and methods for reducing the chance for contamination from manipulation of reagents, internal controls for amplification, and the use of automated apparatus for the automated detection of one, or more than one amplified nucleic acid sequences.
摘要翻译： 本发明涉及通过扩增特异性核酸序列的方法来检测特异性核酸序列。 更具体地，本发明提供了改进的组合物和方法，用于降低操纵试剂的污染机会，用于扩增的内部对照，以及使用用于自动检测一个或多于一个扩增的核酸序列的自动化装置。
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7.发明授权Dual chamber disposable reaction vessel for amplification reactions, reaction processing station therefor, and methods of use 失效用于扩增反应的双室一次性反应容器，其反应处理站及其使用方法
公开(公告)号：US5786182A
公开(公告)日：1998-07-28
申请号：US850207
申请日：1997-05-02
申请人： Luigi Catanzariti , Bryan W. Kluttz , Geoff A. McKinley , Arthur L. Garland , Louis Graziano , James G. Moe , Marcela Vera-Garcia , James Clement Bishop , David Chastain , Fabio Gennari
发明人： Luigi Catanzariti , Bryan W. Kluttz , Geoff A. McKinley , Arthur L. Garland , Louis Graziano , James G. Moe , Marcela Vera-Garcia , James Clement Bishop , David Chastain , Fabio Gennari
CPC分类号： B01L3/502 , B01L7/52 , B01L2200/026 , B01L2300/0825 , B01L2300/0861 , B01L2400/049 , B01L2400/0655
摘要： A reaction vessel for a nucleic acid amplification reaction has a first chamber containing an amplification reagent mix, a second chamber containing an amplification enzyme, and a fluid channel or chamber connecting the first and second chambers together. A fluid sample is introduced into the first chamber. After a denaturation and primer annealing process has occurred in the first chamber, the fluid channel is opened to allow the solution of the reagent and fluid sample to flow into the second chamber. The second chamber is maintained at an optimal temperature for the amplification reaction. A station is described for processing test strips incorporating the reaction vessels. The station includes temperature and vacuum control subsystems to maintain proper temperatures in the reaction vessel and effectuate the transfer of the fluid from one chamber to the other in an automated fashion without human intervention.
摘要翻译： 用于核酸扩增反应的反应容器具有包含扩增试剂混合物的第一室，含有扩增酶的第二室和将第一和第二室连接在一起的流体通道或室。 将流体样品引入第一室。 在第一室中发生变性和引物退火过程之后，打开流体通道以允许试剂和流体样品的溶液流入第二室。 第二个室保持在扩增反应的最佳温度。 描述了一个工位，用于处理加入反应容器的试片。 该站包括温度和真空控制子系统，以在反应容器中保持适当的温度，并且以无需人为干预的方式实现流体从一个室到另一个室的转移。
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公开(公告)号：US07214529B2
公开(公告)日：2007-05-08
申请号：US11180806
申请日：2005-07-12
申请人： Bryan W. Kluttz , Geoff A. McKinley , Fabio Gennari , Michel Guy , Christopher Cotter , Luigi Catanzariti , Louis Graziano , Bruno Colin , Cecile Jaravel , Jacques Dachaud
发明人： Bryan W. Kluttz , Geoff A. McKinley , Fabio Gennari , Michel Guy , Christopher Cotter , Luigi Catanzariti , Louis Graziano , Bruno Colin , Cecile Jaravel , Jacques Dachaud
IPC分类号： C12M1/36
CPC分类号： C12Q1/6848 , B01L3/502 , B01L7/52 , B01L7/525 , B01L2200/026 , B01L2200/16 , B01L2300/044 , B01L2300/0809 , B01L2300/0825 , B01L2300/0861 , B01L2300/087 , B01L2300/1822 , B01L2400/0487 , B01L2400/049 , B01L2400/0633 , B01L2400/0644 , B01L2400/0655 , G01N35/026 , G01N2035/0436
摘要： An instrument for conducting nucleic acid amplification reactions in a disposable test device. The test device includes a first reaction chamber containing a first nucleic acid amplification reagent (e.g., primers and nucleotides) and a second reaction chamber either containing, or in fluid communication, with a second nucleic acid amplification reagent (e.g., an amplification enzyme such as RT). The instrument includes a support structure receiving the test device. A temperature control system maintains the first reaction chamber at a first elevated temperature but simultaneously maintains the second nucleic acid amplification reagent at a second temperature lower than the first temperature so as to preserve the second nucleic acid amplification reagent. An actuator operates on a fluid conduit in the test device to place the first and second reaction chambers in fluid communication with each other after a reaction has occurred in the first reaction chamber at the first temperature. A pneumatic system is also provided that assists in fluid transfer of a reaction solution from the first chamber to the second chamber.
摘要翻译： 用于在一次性测试装置中进行核酸扩增反应的仪器。 测试装置包括含有第一核酸扩增试剂（例如引物和核苷酸）的第一反应室和含有或与第二核酸扩增试剂（例如，扩增酶如 RT）。 仪器包括接收测试装置的支撑结构。 温度控制系统将第一反应室保持在第一升高温度，同时将第二核酸扩增试剂保持在低于第一温度的第二温度，以便保留第二核酸扩增试剂。 致动器在测试装置中的流体导管上操作，以在第一温度下在第一反应室中发生反应之后使第一和第二反应室彼此流体连通。 还提供了一种有助于将反应溶液从第一室流体转移到第二室的气动系统。
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公开(公告)号：US20110020921A1
公开(公告)日：2011-01-27
申请号：US12924351
申请日：2010-09-24
申请人： Bryan W. Kluttz , Geoff A. McKinley , Fabio Gennari , Michel Guy , Christopher Cotter , Luigi Catanzariti , Louis Graziano , Bruno Colin , Cecile Paris , Jacques Dachaud
发明人： Bryan W. Kluttz , Geoff A. McKinley , Fabio Gennari , Michel Guy , Christopher Cotter , Luigi Catanzariti , Louis Graziano , Bruno Colin , Cecile Paris , Jacques Dachaud
IPC分类号： C12M1/34
CPC分类号： C12Q1/6848 , B01L3/502 , B01L7/52 , B01L7/525 , B01L2200/026 , B01L2200/16 , B01L2300/044 , B01L2300/0809 , B01L2300/0825 , B01L2300/0861 , B01L2300/087 , B01L2300/1822 , B01L2400/0487 , B01L2400/049 , B01L2400/0633 , B01L2400/0644 , B01L2400/0655 , G01N35/026 , G01N2035/0436
摘要： An instrument for conducting nucleic acid amplification reactions in a disposable test device. The test device includes a first reaction chamber containing a first nucleic acid amplification reagent (e.g., primers and nucleotides) and a second reaction chamber either containing, or in fluid communication, with a second nucleic acid amplification reagent (e.g., an amplification enzyme such as RT). The instrument includes a support structure receiving the test device. A temperature control system maintains the first reaction chamber at a first elevated temperature but simultaneously maintains the second nucleic acid amplification reagent at a second temperature lower than the first temperature so as to preserve the second nucleic acid amplification reagent. An actuator operates on a fluid conduit in the test device to place the first and second reaction chambers in fluid communication with each other after a reaction has occurred in the first reaction chamber at the first temperature. A pneumatic system is also provided that assists in fluid transfer of a reaction solution from the first chamber to the second chamber.
摘要翻译： 用于在一次性测试装置中进行核酸扩增反应的仪器。 测试装置包括含有第一核酸扩增试剂（例如引物和核苷酸）的第一反应室和含有或与第二核酸扩增试剂（例如，扩增酶如 RT）。 仪器包括接收测试装置的支撑结构。 温度控制系统将第一反应室保持在第一升高温度，同时将第二核酸扩增试剂保持在低于第一温度的第二温度，以便保留第二核酸扩增试剂。 致动器在测试装置中的流体导管上操作，以在第一温度下在第一反应室中发生反应之后使第一和第二反应室彼此流体连通。 还提供了一种有助于将反应溶液从第一室流体转移到第二室的气动系统。
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公开(公告)号：US06949376B2
公开(公告)日：2005-09-27
申请号：US10141049
申请日：2002-05-07
申请人： Bryan W. Kluttz , Geoff A. McKinley , Fabio Gennari , Michel Guy , Christopher Cotter , Luigi Catanzariti , Louis Graziano , Bruno Colin , Cecile Jaravel , Jacques Dachaud
发明人： Bryan W. Kluttz , Geoff A. McKinley , Fabio Gennari , Michel Guy , Christopher Cotter , Luigi Catanzariti , Louis Graziano , Bruno Colin , Cecile Jaravel , Jacques Dachaud
IPC分类号： C12N15/09 , B01J19/00 , B01J19/24 , B01L3/00 , B01L7/00 , C12M20060101 , C12M1/00 , C12M1/18 , C12M1/34 , C12M3/00 , C12Q1/68 , G01N21/00 , G01N33/50 , G01N35/02 , G01N37/00 , C12M1/36
CPC分类号： C12Q1/6848 , B01L3/502 , B01L7/52 , B01L7/525 , B01L2200/026 , B01L2200/16 , B01L2300/044 , B01L2300/0809 , B01L2300/0825 , B01L2300/0861 , B01L2300/087 , B01L2300/1822 , B01L2400/0487 , B01L2400/049 , B01L2400/0633 , B01L2400/0644 , B01L2400/0655 , G01N35/026 , G01N2035/0436
摘要： An instrument for conducting nucleic acid amplification reactions in a disposable test device. The test device includes a first reaction chamber containing a first nucleic acid amplification reagent (e.g., primers and nucleotides) and a second reaction chamber either containing, or in fluid communication, with a second nucleic acid amplification reagent (e.g., an amplification enzyme such as RT). The instrument includes a support structure receiving the test device. A temperature control system maintains the first reaction chamber at a first elevated temperature but simultaneously maintains the second nucleic acid amplification reagent at a second temperature lower than the first temperature so as to preserve the second nucleic acid amplification reagent. An actuator operates on a fluid conduit in the test device to place the first and second reaction chambers in fluid communication with each other after a reaction has occurred in the first reaction chamber at the first temperature. A pneumatic system is also provided that assists in fluid transfer of a reaction solution from the first chamber to the second chamber.
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