Beta-catenin oligonucleotide microchip and method for detecting beta-catenin mutations employing same
    1.
    发明申请
    Beta-catenin oligonucleotide microchip and method for detecting beta-catenin mutations employing same 有权
    β-连环蛋白寡核苷酸微芯片和使用其检测β-连环蛋白突变的方法

    公开(公告)号:US20050176016A1

    公开(公告)日:2005-08-11

    申请号:US10659583

    申请日:2003-09-09

    摘要: The present invention relates to a β-catenin oligonucleotide microchip for detecting mutation in the mutational hot spot regions of β-catenin gene, a manufacturing process thereof and a method for detecting the β-catenin mutation employing same, wherein specific oligonucleotides are selectively designed to detect various missense mutations and in-frame deletion at the mutational hot spots of β-catenin gene. The β-catenin oligo chip of the present invention can be used in studies to detect β-catenin mutations and unravel the signal transduction mechanism and tumorigenesis related to β-catenin gene.

    摘要翻译: 本发明涉及用于检测β-连环蛋白基因的突变热点区域中的突变的β-连环蛋白寡核苷酸微芯片,其制备方法和使用其的β-连环蛋白突变检测方法,其中特异性寡核苷酸被选择性地设计成 在β-连环蛋白基因的突变热点检测各种错义突变和帧内缺失。 本发明的β-连环蛋白寡核苷酸芯片可用于检测β-连环蛋白突变和揭示与β-连环蛋白基因相关的信号转导机制和肿瘤发生的研究。

    K-Ras Oligonucleotide Microarray and Method for Detecting K-Ras Mutations Employing the Same
    2.
    发明申请
    K-Ras Oligonucleotide Microarray and Method for Detecting K-Ras Mutations Employing the Same 审中-公开
    K-Ras寡核苷酸微阵列和检测使用它的K-Ras突变的方法

    公开(公告)号:US20070298419A1

    公开(公告)日:2007-12-27

    申请号:US11587569

    申请日:2004-11-22

    IPC分类号: C12Q1/68 C12M1/00

    摘要: Since the K-ras oligonucleotide microarray of the present invention can detect K-ras mutations by applying a competitive DNA hybridization method to the oligonucleotides spotted on a solid matrix different from the previously reported method for detecting a mutation, it makes the more precise analysis and can reduce experimental cost and time. Accordingly, the K-ras oligonucleotide microarray of the present invention can be used in studies to detect K-ras mutations and unravel the signal transduction mechanism and tumorigenesis related to K-ras gene. Further, since the microarray of the present invention can be applied to other genes having mutational hot spot regions such as K-ras, it has wide applicable range.

    摘要翻译: 由于本发明的K-ras寡核苷酸微阵列可以通过将竞争性DNA杂交方法应用于与先前报道的用于检测突变的方法不同的固体基质上的寡核苷酸来检测K-ras突变,从而进行更精确的分析和 可以降低实验成本和时间。 因此,本发明的K-ras寡核苷酸微阵列可用于检测K-ras突变和揭示与K-ras基因相关的信号转导机制和肿瘤发生的研究。 此外,由于本发明的微阵列可以应用于具有K-ras等突变热点区域的其他基因,因此具有广泛的适用范围。

    β-catenin oligonucleotide microchip and method for detecting β-catenin mutations employing same
    3.
    发明授权
    β-catenin oligonucleotide microchip and method for detecting β-catenin mutations employing same 有权
    β-连环蛋白寡核苷酸微芯片和使用其检测β-连环蛋白突变的方法

    公开(公告)号:US07312070B2

    公开(公告)日:2007-12-25

    申请号:US10659583

    申请日:2003-09-09

    IPC分类号: C12Q1/68 C12M1/38 C07H21/04

    摘要: The present invention relates to a β-catenin oligonucleotide microchip for detecting mutation in the mutational hot spot regions of β-catenin gene, a manufacturing process thereof and a method for detecting the β-catenin mutation employing same, wherein specific oligonucleotides are selectively designed to detect various missense mutations and in-frame deletion at the mutational hot spots of β-catenin gene. The β-catenin oligo chip of the present invention can be used in studies to detect β-catenin mutations and unravel the signal transduction mechanism and tumorigenesis related to β-catenin gene.

    摘要翻译: 本发明涉及用于检测β-连环蛋白基因的突变热点区域中的突变的β-连环蛋白寡核苷酸微芯片,其制备方法和使用其的β-连环蛋白突变检测方法,其中特异性寡核苷酸被选择性地设计成 在β-连环蛋白基因的突变热点检测各种错义突变和帧内缺失。 本发明的β-连环蛋白寡核苷酸芯片可用于检测β-连环蛋白突变和揭示与β-连环蛋白基因相关的信号转导机制和肿瘤发生的研究。