Nucleic acid detection system
    1.
    发明申请
    Nucleic acid detection system 审中-公开
    核酸检测系统

    公开(公告)号:US20050227275A1

    公开(公告)日:2005-10-13

    申请号:US11102001

    申请日:2005-04-07

    IPC分类号: C12Q1/68

    摘要: The present application discloses a system for detecting target nucleic acid comprising: a container comprising a nucleic acid amplification mix comprising a primer labeled with different haptens at its 5′ and 3′ ends, and optionally dNTP labeled with a hapten to form a nucleic acid complex; and a lateral flow test device comprising a reservoir area comprising reagent conditions suitable for binding of a specific binding partner with the nucleic acid complex; a dye area comprising a specific binding partner to the nucleic acid complex, wherein the specific binding partner is linked or conjugated to a reporter dye or another hapten; and a test area comprising a different specific binding partner specific to a different aspect of the nucleic acid complex.

    摘要翻译: 本申请公开了一种用于检测靶核酸的系统,包括:容器,其包含核酸扩增混合物,其包含在其5'和3'末端用不同半抗原标记的引物,以及任选地用半抗原标记的dNTP以形成核酸复合物 ; 和侧流测试装置,其包括储存区域,其包含适合于将特异性结合配偶体与核酸复合物结合的试剂条件; 染料区域,其包含与所述核酸复合物的特异性结合配偶体,其中所述特异性结合配偶体与报告染料或其他半抗原连接或缀合; 以及包含对核酸复合物的不同方面特异的不同特异性结合伴侣的测试区域。