Pseudomonas chlororaphis microorganism, polyurethane degrading enzyme obtained therefrom, and method of use
    4.
    发明授权
    Pseudomonas chlororaphis microorganism, polyurethane degrading enzyme obtained therefrom, and method of use 失效
    绿脓假单胞菌,得自其的聚氨酯降解酶及其使用方法

    公开(公告)号:US06180381B2

    公开(公告)日:2001-01-30

    申请号:US09016296

    申请日:1998-01-30

    IPC分类号: C12N914

    CPC分类号: C12R1/38 C12N9/00

    摘要: Wild-type bacteria with polyurethanase activity, and the ability to excrete polyurethanase into a culture medium have been genetically altered to significantly increase their polyurethanase activity. Biologically pure cultures of these genetically altered are cultured in a medium containing an inducer for the production of polyurethanase. The bacteria are then removed from the culture medium to provide a preparation with a high polyurethanase activity. One useful genetically altered bacteria is a strain of Pseudomonas chlororaphis designated ATTC No. 55729. The polyurethanase-containing preparation may then be used directly to degrade polyurethane, particularly polyurethane and polyurethane-based coatings.

    摘要翻译: 具有聚氨酯酶活性的野生型细菌和将聚氨酯酶分泌到培养基中的能力已被遗传改变以显着增加其聚氨酯酶活性。 将这些遗传改变的生物学纯培养物在含有用于生产聚氨酯酶的诱导剂的培养基中培养。 然后从培养基中除去细菌以提供具有高聚氨酯酶活性的制剂。 一种有用的遗传改变的细菌是命名为ATTC No.55729的绿脓假单胞菌菌株。然后可以直接使用含聚氨酯酶的制剂降解聚氨酯,特别是聚氨酯和聚氨酯基涂料。

    Method for detecting biological toxins
    5.
    依法登记的发明

    公开(公告)号:USH1985H1

    公开(公告)日:2001-08-07

    申请号:US07818600

    申请日:1992-01-09

    IPC分类号: C12Q168

    摘要: Biological toxins are indirectly detected by using polymerase chain reaction to amplify unique nucleic acid sequences coding for the toxins or enzymes unique to toxin synthesis. Buffer, primers coding for the unique nucleic acid sequences and an amplifying enzyme are added to a sample suspected of containing the toxin. The mixture is then cycled thermally to exponentially amplify any of these unique nucleic acid sequences present in the sample. The amplified sequences can be detected by various means, including fluorescence. Detection of the amplified sequences is indicative of the presence of toxin in the original sample. By using more than one set of labeled primers, the method can be used to simultaneously detect several toxins in a sample.

    Apparatus for fabricating and welding core reinforced panel
    6.
    发明授权
    Apparatus for fabricating and welding core reinforced panel 失效
    核心加强板的制造和焊接设备

    公开(公告)号:US4280039A

    公开(公告)日:1981-07-21

    申请号:US2761

    申请日:1979-01-12

    IPC分类号: B23K11/10 B23K11/00 B23K37/02

    CPC分类号: B23K11/0093

    摘要: A machine or apparatus simultaneously manufacutures a core-reinforced welded panel and assembles core strips in operative relationship with the face sheets of said panel. The machine includes a first welding head which is insertable in the space between the face sheets and has welding electrode means thereupon which internally weld securement portions of the core strips to adjacent inner surfaces of face sheets of any desired thickness. The welding head includes movable jaws mounting the welding electrodes to move the welding electrodes into engagement with the securement portions of the core strips and the welding electrodes are resilient to permit them to move independently of the movable jaws and each electrode provides an electrically isolated weld. A second welding head is provided which is capable of securing web portions of the core strips by weldments to one another after the weldment of the core strips at their securement portions has been accomplished by the first welding head. Various adjustments and indexing mechanisms permit the machine to fabricate panels of different dimensions and different size core without the necessity for the extensive down-time necessitated by the utilization of prior art machines.

    摘要翻译: 机器或设备同时制造核心加强的焊接面板并且组装与所述面板的面板操作关系的芯条。 该机器包括可插入面板之间的空间中的第一焊接头,并且具有焊接电极装置,其内部将芯条的固定部分焊接到任何所需厚度的面板的相邻内表面。 焊头包括安装焊接电极的可动爪,以移动焊接电极与芯条的固定部分接合,并且焊接电极具有弹性,以允许它们独立于可移动夹爪移动,并且每个电极提供电隔离焊接。 提供了一种第二焊接头,其能够通过第一焊接头实现在其固定部分处的芯条的焊接之后通过焊件将芯体条的网部分固定。 各种调节和分度机构允许机器制造不同尺寸和不同尺寸的芯的面板,而不需要通过利用现有技术的机器所需的广泛的停机时间。

    DNA-based fluourescent sensor
    8.
    依法登记的发明
    DNA-based fluourescent sensor 失效
    基于DNA的荧光传感器

    公开(公告)号:USH1398H

    公开(公告)日:1995-01-03

    申请号:US932999

    申请日:1992-08-21

    申请人: James R. Campbell

    发明人: James R. Campbell

    IPC分类号: C12Q1/68 C12Q1/00

    CPC分类号: C12Q1/686 C12Q1/6816

    摘要: The fluorescent detection of target DNA is enhanced by amplifying the DNA in a sample by initiating a polymerase chain reaction using a first pair of primers and an appropriate polymerase. The DNA is then reamplified, this time using a second pair of nested primers which bracket DNA base sequences within the region bracketed by the first set of primers. At least one of the second pair of primers is bound to a fluorescent reporter molecule and at least one of the second pair of primers has bound thereto a specific binding site for a double-stranded DNA-binding protein. The thus amplified sample is then contacted with a fluorescent sensor having the appropriate double-stranded DNA-binding protein attached thereto. Then, the fluorescent reporter molecule is excited by light of the appropriate wavelength. The present invention provides greater sensitivity, adaptability and ease of use than previously available.

    摘要翻译: 通过使用第一对引物和适当的聚合酶引发聚合酶链反应,扩增样品中的DNA来增强目标DNA的荧光检测。 然后将DNA重新扩增,这次使用第二对嵌套引物,其将由第一组引物括起的区域内的DNA碱基序列置换。 第二对引物中的至少一个与荧光报道分子结合,并且第二对引物中的至少一个与双链DNA结合蛋白的特异性结合位点结合。 然后将如此扩增的样品与附着有适当的双链DNA结合蛋白的荧光传感器接触。 然后,荧光报道分子被适当波长的光激发。 本发明提供比以前更高的灵敏度,适应性和易用性。

    Method and apparatus for fabricating honeycomb seal
    10.
    发明授权
    Method and apparatus for fabricating honeycomb seal 失效
    用于制造蜂窝密封件的方法和装置

    公开(公告)号:US4551604A

    公开(公告)日:1985-11-05

    申请号:US503542

    申请日:1983-06-13

    申请人: James R. Campbell

    发明人: James R. Campbell

    IPC分类号: B23K11/00 B23K11/06 B23K11/32

    CPC分类号: B23K11/0093

    摘要: An apparatus is provided for forming a circular, honeycomb-type engine seal from pre-formed core strip having male and female nodes. The apparatus includes a source of strip and means for forming the strip into a circular seal configuration wherein the male and female nodes of the strip are automatically juxtaposed to each other for weldment as relative movement between the forming means and the source occurs to withdraw additional strip from the core strip source. As the relative movement continues, the juxtaposed male and female nodes are exposed to welding head means of the apparatus which welds the male and female nodes to one another. The forming means includes means for causing said relative movement constituted by drive means which is adjustable to permit forming means of different sizes to be utilized. The movement of the forming means permits successive layers or laminae of the strip to be welded to each other in circular form until a seal of the desired dimension is achieved. The method of the invention involves the step of simultaneously welding a plurality of male and female nodes which are juxtaposed to each other, and the additional step of welding pairs of nodes which are interspersed between previously welded pairs and the further step of utilizing the welding of a selected pair of nodes as a signal for the simultaneous weldment of additional pairs.

    摘要翻译: 提供了一种用于从具有阳节点和阴节点的预成形芯条形成圆形蜂窝型发动机密封件的装置。 该设备包括条带源和用于将条带形成为圆形密封结构的装置,其中,当形成装置和源之间的相对运动发生时,条带的阳和阴节点自动并置到彼此以进行焊接, 从核心带源。 随着相对运动的继续,并置男性和女性节点暴露于将男性和女性节点彼此焊接的设备的焊接头装置。 成形装置包括用于使由驱动装置构成的所述相对运动的装置,该驱动装置可调节以允许使用不同尺寸的成形装置。 成形装置的移动允许条带的连续层或薄片以圆形形式彼此焊接,直到实现所需尺寸的密封。 本发明的方法包括同时焊接彼此并置的多个阳性和阴性节点的步骤,以及焊接成对的先前焊接的对之间的节点对的另外的步骤以及利用焊接的另一个步骤 选择的一对节点作为用于同时焊接附加对的信号。