公开(公告)号：US07056687B2

公开(公告)日：2006-06-06

申请号：US10096339

申请日：2002-03-08

申请人： James Lorens ,  Todd M. Kinsella ,  Esteban Masuda ,  Yasumichi Hitoshi ,  X. Charlene Liao ,  Denise Pearsall ,  Annabelle Freiro ,  Peter Chu

发明人： James Lorens ,  Todd M. Kinsella ,  Esteban Masuda ,  Yasumichi Hitoshi ,  X. Charlene Liao ,  Denise Pearsall ,  Annabelle Freiro ,  Peter Chu

IPC分类号： G01N33/53 ,  G01N33/567 ,  G01N33/542 ,  C12N15/12 ,  C12Q1/66

CPC分类号： C12N15/1079 ,  C07K14/70578 ,  C07K2319/00 ,  C12N15/63 ,  C12N2510/00 ,  C12Q1/6897 ,  G01N33/5008 ,  G01N33/5023 ,  G01N33/5047 ,  G01N2500/10

摘要： The invention relates to methods and compositions useful for screening for altered cellular phenotypes using an inducible expression system to enrich for and detect the altered phenotypes and, more particularly, relates to screening libraries of candidate bioactive agents, for example, nucleic acids and peptides, in cells using an regulatable expression system to enrich for a subpopulation of cells having an altered phenotype due to the presence of a candidate bioactive agent.

公开(公告)号：US20060147978A1

公开(公告)日：2006-07-06

申请号：US11361631

申请日：2006-02-24

申请人： James Lorens ,  Todd Kinsella ,  Esteban Masuda ,  Yasumichi Hitoshi ,  X. Liao ,  Denise Pearsall ,  Annabelle Friera ,  Peter Chu

发明人： James Lorens ,  Todd Kinsella ,  Esteban Masuda ,  Yasumichi Hitoshi ,  X. Liao ,  Denise Pearsall ,  Annabelle Friera ,  Peter Chu

IPC分类号： C12Q1/68 ,  G01N33/567

CPC分类号： C12N15/1079 ,  C07K14/70578 ,  C07K2319/00 ,  C12N15/63 ,  C12N2510/00 ,  C12Q1/6897 ,  G01N33/5008 ,  G01N33/5023 ,  G01N33/5047 ,  G01N2500/10

摘要： The invention relates to methods and compositions useful for screening for altered cellular phenotypes using an inducible expression system to enrich for and detect the altered phenotypes and, more particularly, relates to screening libraries of candidate bioactive agents, for example, nucleic acids and peptides, in cells using an regulatable expression system to enrich for a subpopulation of cells having an altered phenotype due to the presence of a candidate bioactive agent.

公开(公告)号：US07005254B2

公开(公告)日：2006-02-28

申请号：US10160663

申请日：2002-05-31

申请人： Susan Demo ,  Yasumichi Hitoshi ,  Denise Pearsall

发明人： Susan Demo ,  Yasumichi Hitoshi ,  Denise Pearsall

IPC分类号： C12Q1/00 ,  C12Q1/68 ,  G01N33/53

CPC分类号： C07K14/47 ,  G01N33/574 ,  G01N2333/705 ,  G01N2500/04

摘要： The present invention relates to regulation of cellular proliferation. More particularly, the present invention is directed to nucleic acids encoding LETM1 (“leucine zipper EF hand transmembrane receptor”), which is involved in modulation of cellular proliferation and cell cycle regulation. The invention further relates to methods for identifying and using agents, including small molecule chemical compositions, antibodies, siRNA, antisense nucleic acids, and ribozymes, that modulate cell cycle regulation and cellular proliferation via modulation of LETM1 and LETM1 related signal transduction; as well as to the use of expression profiles and compositions in diagnosis and therapy related to cell cycle regulation and modulation of cellular proliferation.