利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

26 条记录 (耗时 0.049 秒)

    shRNA library
    3.
    发明申请
    shRNA library 有权
    shRNA文库

    公开(公告)号：US20100029505A1

    公开(公告)日：2010-02-04

    申请号：US12221325

    申请日：2008-08-01

    申请人： Donald G. Payan ,  Mary Shen ,  Simon Yu ,  Todd M. Kinsella ,  Yasumichi Hitoshi

    发明人： Donald G. Payan ,  Mary Shen ,  Simon Yu ,  Todd M. Kinsella ,  Yasumichi Hitoshi

    IPC分类号： C40B30/06 ,  C40B40/08 ,  C40B50/06 ,  C12P19/34 ,  C40B40/02

    CPC分类号： C12N15/1093 ,  C12N15/66 ,  C12P19/34 ,  C40B40/08 ,  C40B50/06

    摘要： As noted above, certain aspects of this disclosure relate to a library of nucleic acid vectors, as well as a method for making the same. In certain embodiments, the library of nucleic acid vectors comprises: a plurality of nucleic acid molecules of the following formula: S1—R—S2 wherein, in each nucleic acid of the plurality: S1 and S2 are each at least 15 nucleotides in length; S1 and S2 are complementary to each other along their entire length; either S1 or S2 is complementary along its entire length to a sequence in eukaryotic mRNA; and R is a six base recognition site for a restriction endonuclease; and wherein S1 and S2 vary in nucleotide sequence between different members of the plurality. A method for amplifying a circular nucleic acid is also provided.

    摘要翻译： 如上所述，本公开的某些方面涉及核酸载体文库以及其制备方法。 在某些实施方案中，核酸载体文库包含：多个下式的核酸分子：S1-R-S2，其中在多个的每个核酸中，S1和S2的长度各自为至少15个核苷酸; S1和S2沿其整个长度彼此互补; S1或S2在其整个长度上与真核mRNA中的序列互补; R是限制性内切核酸酶的六碱基识别位点; 并且其中S1和S2在多个不同成员之间的核苷酸序列变化。 还提供了扩增环状核酸的方法。

    shRNA library
    4.
    发明授权
    shRNA library 有权
    shRNA文库

    公开(公告)号：US07863222B2

    公开(公告)日：2011-01-04

    申请号：US12221325

    申请日：2008-08-01

    申请人： Donald G. Payan ,  Mary Shen ,  Simon Yu ,  Todd M. Kinsella ,  Yasumichi Hitoshi

    发明人： Donald G. Payan ,  Mary Shen ,  Simon Yu ,  Todd M. Kinsella ,  Yasumichi Hitoshi

    IPC分类号： C40B50/06 ,  C40B40/08 ,  C40B30/06 ,  C40B40/02 ,  C12P19/34 ,  A61K49/12

    CPC分类号： C12N15/1093 ,  C12N15/66 ,  C12P19/34 ,  C40B40/08 ,  C40B50/06

    摘要： As noted above, certain aspects of this disclosure relate to a library of nucleic acid vectors, as well as a method for making the same. In certain embodiments, the library of nucleic acid vectors comprises: a plurality of nucleic acid molecules of the following formula: S1—R—S2 wherein, in each nucleic acid of the plurality: S1 and S2 are each at least 15 nucleotides in length; S1 and S2 are complementary to each other along their entire length; either S1 or S2 is complementary along its entire length to a sequence in eukaryotic mRNA; and R is a six base recognition site for a restriction endonuclease; and wherein S1 and S2 vary in nucleotide sequence between different members of the plurality. A method for amplifying a circular nucleic acid is also provided.

    摘要翻译： 如上所述，本公开的某些方面涉及核酸载体文库以及其制备方法。 在某些实施方案中，核酸载体文库包含：多个下式的核酸分子：S1-R-S2，其中在多个的每个核酸中，S1和S2的长度各自为至少15个核苷酸; S1和S2沿其整个长度彼此互补; S1或S2在其整个长度上与真核mRNA中的序列互补; R是限制性内切核酸酶的六碱基识别位点; 并且其中S1和S2在多个不同成员之间的核苷酸序列变化。 还提供了扩增环状核酸的方法。

    LETM1: modulators of cellular proliferation
    5.
    发明授权
    LETM1: modulators of cellular proliferation 有权

    公开(公告)号：US07005254B2

    公开(公告)日：2006-02-28

    申请号：US10160663

    申请日：2002-05-31

    申请人： Susan Demo ,  Yasumichi Hitoshi ,  Denise Pearsall

    发明人： Susan Demo ,  Yasumichi Hitoshi ,  Denise Pearsall

    IPC分类号： C12Q1/00 ,  C12Q1/68 ,  G01N33/53

    CPC分类号： C07K14/47 ,  G01N33/574 ,  G01N2333/705 ,  G01N2500/04

    摘要： The present invention relates to regulation of cellular proliferation. More particularly, the present invention is directed to nucleic acids encoding LETM1 (“leucine zipper EF hand transmembrane receptor”), which is involved in modulation of cellular proliferation and cell cycle regulation. The invention further relates to methods for identifying and using agents, including small molecule chemical compositions, antibodies, siRNA, antisense nucleic acids, and ribozymes, that modulate cell cycle regulation and cellular proliferation via modulation of LETM1 and LETM1 related signal transduction; as well as to the use of expression profiles and compositions in diagnosis and therapy related to cell cycle regulation and modulation of cellular proliferation.

    SAK: modulation of cellular proliferation for treatment of cancer
    8.
    发明授权
    SAK: modulation of cellular proliferation for treatment of cancer 失效
    SAK：调节细胞增殖治疗癌症

    公开(公告)号：US07413870B2

    公开(公告)日：2008-08-19

    申请号：US10026021

    申请日：2001-12-21

    申请人： Yasumichi Hitoshi ,  Susan Demo ,  Yonchu Jenkins

    发明人： Yasumichi Hitoshi ,  Susan Demo ,  Yonchu Jenkins

    IPC分类号： C12Q1/50

    CPC分类号： G01N33/57484 ,  A61K38/00 ,  A61K2039/505 ,  G01N2333/912

    摘要： The present invention relates to regulation of cellular proliferation. More particularly, the present invention is directed to nucleic acids encoding SAK, which is a protein kinase involved in modulation of cellular proliferation and cell cycle regulation. The invention further relates to methods for identifying and using agents, including small molecule chemical compositions, antibodies, peptides, cyclic peptides, nucleic acids, RNAi, antisense nucleic acids, and ribozymes, that modulate cell cycle regulation and cellular proliferation via modulation of SAK; as well as to the use of expression profiles and compositions in diagnosis and therapy related to cell cycle regulation and modulation of cellular proliferation, e.g., for treatment of cancer and other diseases of cellular proliferation.

    摘要翻译： 本发明涉及细胞增殖的调控。 更具体地，本发明涉及编码SAK的核酸，其是参与调节细胞增殖和细胞周期调节的蛋白激酶。 本发明还涉及用于鉴别和使用通过调节SAK调节细胞周期调节和细胞增殖的试剂，包括小分子化学组合物，抗体，肽，环肽，核酸，RNAi，反义核酸和核酶的方法; 以及在细胞周期调控和细胞增殖调节相关的诊断和治疗中使用表达谱和组合物，例如用于治疗癌症和其它细胞增殖疾病。

    Methods of assaying for cell cycle modulators
    9.
    发明申请
    Methods of assaying for cell cycle modulators 审中-公开
    测定细胞周期调节剂的方法

    公开(公告)号：US20060051755A1

    公开(公告)日：2006-03-09

    申请号：US10510903

    申请日：2003-04-15

    申请人： Yasumichi Hitoshi ,  Yonchu Jenkins

    发明人： Yasumichi Hitoshi ,  Yonchu Jenkins

    IPC分类号： C12Q1/68

    CPC分类号： G01N33/5091 ,  C12Q1/6886 ,  C12Q2600/158 ,  G01N33/5008 ,  G01N33/5011 ,  G01N33/574 ,  G01N33/6872 ,  G01N2333/4703 ,  G01N2500/00

    摘要： The present invention relates to regulation of cellular proliferation. More particularly, the present invention is directed to nucleic acids encoding BRCA-1-Associated Protein-1 (BAP-1), Nuclear Protein 95 (NP95), Fanconi anemia group A protein (FANCA), DEAD/H box polypeptide 9 (DDX9), insulin-like growth factor 1 receptor (IGF1R), ubiquitin-conjugating enzyme E2 variant 1 (UBE2V1), aldehyde dehydrogenase, pyruvate kinase, glucose-6-phosphate dehydrogenase, HCDR-3, DEAD/H box polypeptide 21 (DDX21), serine threonine kinase 15 (ARK2), transmembrane 4 superfamily member 1, or ERCC1, which are involved in modulation of cell cycle arrest. The invention further relates to methods for identifying and using agents, including small molecule chemical compositions, antibodies, peptides, cyclic peptides, nucleic acids, RNAi, antisense nucleic acids, and ribozymes, that modulate cell cycle arrest via modulation of BRCA-1-Associated Protein-1 (BAP-1), Nuclear Protein 95 (NP95), Fanconi anemia group A protein (FANCA), DEAD/H box polypeptide 9 (DDX9), insulin-like growth factor 1 receptor (IGF1R), ubiquitin-conjugating enzyme E2 variant 1 (UBE2V1), aldehyde dehydrogenase, pyruvate kinase, glucose-6-phosphate dehydrogenase, HCDR-3, DEAD/H box polypeptide 21 (DDX21), serine threonine kinase 15 (ARK2), transmembrane 4 superfamily member 1, or ERCC1, as well as to the use of expression profiles and compositions in diagnosis and therapy related to cell cycle regulation and modulation of cellular proliferation, e.g., for treatment of cancer and other diseases of cellular proliferation.

    摘要翻译： 本发明涉及细胞增殖的调控。 更具体地，本发明涉及编码BRCA-1相关蛋白-1（BAP-1），核蛋白95（NP95），Fanconi贫血A组蛋白（FANCA），DEAD / H盒多肽9（DDX9） ），胰岛素样生长因子1受体（IGF1R），泛素结合酶E2变体1（UBE2V1），醛脱氢酶，丙酮酸激酶，葡萄糖-6-磷酸脱氢酶，HCDR-3，DEAD / H盒多肽21（DDX21） ，丝氨酸苏氨酸激酶15（ARK2），跨膜4超家族成员1或ERCC1，其参与调节细胞周期停滞。 本发明还涉及用于鉴定和使用包括小分子化学组合物，抗体，肽，环肽，核酸，RNAi，反义核酸和核酶在内的试剂的方法，其通过调节BRCA-1相关的调节细胞周期阻滞 蛋白-1（BAP-1），核蛋白95（NP95），Fanconi贫血组A蛋白（FANCA），DEAD / H盒多肽9（DDX9），胰岛素样生长因子1受体（IGF1R），泛素缀合酶 E2变体1（UBE2V1），醛脱氢酶，丙酮酸激酶，葡萄糖-6-磷酸脱氢酶，HCDR-3，DEAD / H盒多肽21（DDX21），丝氨酸苏氨酸激酶15（ARK2），跨膜4超家族成员1或ERCC1 ，以及在与细胞周期调节和细胞增殖调节相关的诊断和治疗中使用表达谱和组合物，例如用于治疗癌症和其他细胞增殖疾病。