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公开(公告)号：US07575918B2
公开(公告)日：2009-08-18
申请号：US10935962
申请日：2004-09-08
申请人： James Norris , Gary Clawson , Caroline Westwater , David Schofield , Michael G. Schmidt , Brian Hoel , Joseph Dolan , Wei-Hua Pan
发明人： James Norris , Gary Clawson , Caroline Westwater , David Schofield , Michael G. Schmidt , Brian Hoel , Joseph Dolan , Wei-Hua Pan
CPC分类号： C08K5/20 , A61K38/00 , A61K48/00 , C08K5/29 , C12N15/113 , C12N15/74 , C12N2310/111 , C12N2310/12 , C12N2310/317 , C12N2799/021 , C08L69/00
摘要： The present invention relates to the discovery, identification and characterization of toxic agents which are lethal to pathogens and methods for targeting such toxic agents to a pathogen or pathogen infected cells in order to treat and/or eradicate the infection. In particular, the present invention relates to toxic agents which target bacteria at different stages of the bacterial life cycle, which are delivered alone or in combination to bacteria or bacteria-infected cells. The invention relates to toxic agents which are lethal to diseased cells and methods for targeting such toxic agents to a diseased cell in order to treat and/or eradicate the disease. The present invention relates to promoter elements which are pathogen-specific or tissue-specific and the use of such promoter elements to achieve pathogen-specific or tissue-specific expression of the toxic agent(s) and/or ribozyme(s) of the present invention. Specifically, the invention relates to the delivery of one or more toxic gene products, antisense RNAs, or ribozymes, or combination thereof. The invention provides a novel system by which multiple pathogenic targets may be simultaneously targeted to cause the death of a pathogen, or cell infected with a pathogen. Further, the invention has important implications in the eradication of drug-resistant bacterium and bacterial pathogens. The invention provides a novel system by which multiple targets may be simultaneously targeted to cause the death of a diseased cell. The invention has important implications in the eradication of drug-resistant pathogens (such as antibiotic resistant bacteria) and drug-resistant diseased cells (such as drug-resistant cancer cells).
摘要翻译： 本发明涉及对病原体致死的毒性试剂的发现，鉴定和表征以及将这些毒性试剂靶向病原体或病原体感染的细胞以便治疗和/或根除感染的方法。 特别地，本发明涉及针对细菌生命周期不同阶段的细菌的毒性试剂，它们单独或组合递送至细菌或细菌感染细胞。 本发明涉及对患病细胞致死的毒物剂和用于将这些毒物剂靶向病变细胞以便治疗和/或根除疾病的方法。 本发明涉及作为病原体特异性或组织特异性的启动子元件以及这些启动子元件用于实现本发明的毒性剂和/或核酶的病原体特异性或组织特异性表达的启动子元件 发明。 具体地，本发明涉及递送一种或多种有毒基因产物，反义RNA或核酶或其组合。 本发明提供了一种新的系统，通过该系统可以同时靶向多种致病性靶标以引起病原体或感染病原体的细胞的死亡。 此外，本发明对消除耐药细菌和细菌病原体具有重要的意义。 本发明提供了一种新颖的系统，通过该系统可以同时靶向多个靶标以引起患病细胞的死亡。 本发明对消除耐药性病原体（如抗生素抗性细菌）和耐药性疾病细胞（如耐药性癌细胞）具有重要意义。
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公开(公告)号：US07732197B2
公开(公告)日：2010-06-08
申请号：US10082973
申请日：2002-02-26
申请人： James S. Norris , Gary A. Clawson , Michael G. Schmidt , Brian Hoel , Wei-Hua Pan , Joseph W. Dolan
发明人： James S. Norris , Gary A. Clawson , Michael G. Schmidt , Brian Hoel , Wei-Hua Pan , Joseph W. Dolan
CPC分类号： C12N15/113 , A61K38/00 , C12N2310/111 , C12N2310/121 , C12N2310/127 , C12N2799/021
摘要： The present invention relates to multi-ribozymes and their use to target RNA in a tissue-specific, target RNA-specific, or pathogen-specific manner for the treatment of cancers, proliferative disease, and bacterial, parasitic and viral infections. More specifically, the present invention relates to the use of virions and viral vectors to package and deliver DNA encoding the multi-ribozymes to a host. The present invention relates to the use of liposomes and lipid-DNA complexes to deliver DNA encoding ribozymes to a host. Most specifically, the invention relates to the use of target specific virions to package and deliver DNA comprising a target specific promoter and encoding a ribozyme(s) directed to the target organism nucleic acids. The present invention further relates to a novel vectors encoding a multi-ribozyme structure with enhanced 5′ and/or 3′ autocatalytically cleaving ribozymes. The invention further relates to nucleotides encoding a multi-ribozyme comprising one or more ribozyme cassettes which contain one or more trans-acting ribozymes and one or more autocatalytically cleaving ribozyme sequences.
摘要翻译： 本发明涉及多核酶及其用于以组织特异性，靶RNA特异性或病原体特异性方式靶向用于治疗癌症，增殖性疾病和细菌，寄生虫和病毒感染的RNA的用途。 更具体地，本发明涉及病毒粒子和病毒载体用于将编码多核酶的DNA包装并递送至宿主的用途。 本发明涉及脂质体和脂质DNA复合物将编码核酶的DNA递送至宿主的用途。 最具体地，本发明涉及靶特异性病毒体用于包装和递送包含靶特异性启动子并编码针对靶生物核酸的核酶的DNA的用途。 本发明还涉及编码具有增强的5'和/或3'自动催化裂解核酶的多核酶结构的新载体。 本发明还涉及编码多核酶的核苷酸，其包含一个或多个含有一个或多个反式作用核酶和一个或多个自动催化裂解核酶序列的核酶盒。
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公开(公告)号：US5957143A
公开(公告)日：1999-09-28
申请号：US953443
申请日：1997-10-17
申请人： Michael G. Schmidt
发明人： Michael G. Schmidt
CPC分类号： C11D3/042 , B08B9/28 , C11D11/0035 , C11D3/33 , C11D3/36
摘要： In the case of a process for the mechanical cleaning of reusable drinks containers made of glass or plastic, the process comprising one or more washing cycles or zones and rinsing cycles or zones in a cleaning installation, the reusable drinks containers undergo pretreatment before being supplied to the actual cleaning installation. The reusable drinks containers are sprayed with an aqueous cleaning formulation by means of one or more nozzles which produce a spray yet which is directed into the interior of the reusable containers and, in the interior of the reusable containers, is transformed into a spray mist, with the result that the cleaning formulation is distributed uniformly. The pretreatment ensures that even stubborn dirt or microorganisms such as mold or yeasts is/are removed effectively in the cleaning installation.
摘要翻译： 在用于机械清洁由玻璃或塑料制成的可重复使用的饮料容器的方法的情况下，该方法包括在清洁装置中的一个或多个洗涤循环或区域以及漂洗循环或区域，可再用饮料容器经过预处理，然后供应至 实际清洗安装。 可再利用的饮料容器通过一个或多个喷嘴喷射水性清洁配方，这些喷嘴产生喷雾，其被引导到可重复使用的容器的内部，并且在可重复使用的容器的内部被转化成喷雾， 结果清洁制剂均匀分布。 预处理确保在清洁装置中甚至有效地去除甚至顽固的污垢或微生物如霉菌或酵母。
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公开(公告)号：US6014994A
公开(公告)日：2000-01-18
申请号：US152781
申请日：1998-09-14
申请人： Michael G. Schmidt
发明人： Michael G. Schmidt
CPC分类号： B67C3/001 , B08B9/0325
摘要： The operation of filling plants for filling drinks or other foods is subject to stringent regulations under the law on foodstuffs. In this connection, an essential aspect is the cleanness of the filling plant, which consists of a filler and a conveyor. To clean a filling plant, it is known to provide four separate cleaning systems with four separate supply stations and control systems as well as line systems.The invention provides, for all partial cleaning systems in a common central cleaning circuit (40a, 57), a common supply station (10) comprising the componentsfresh water container (12)storage containers (13-16)heating station (29)dosing station (27),which can selectively be connected to or disconnected from the cleaning circuit by means of valves and rotary valve flaps, as well as a programme control system (11) as well as a common partial line system.
摘要翻译： 填充饮料或其他食品的灌装厂的操作受食品法规的严格管理。 在这方面，一个重要的方面是灌装设备的清洁度，其由填料和输送机组成。 为了清洁灌装厂，已知可以提供四个独立的清洁系统，四个独立的供应站和控制系统以及管线系统。 本发明为共同的中央清洁回路（40a，57）中的所有部分清洁系统提供了一个共同供应站（10），其包括部件淡水容器（12）储存容器（13-16）加热站（29）给料 站（27），其可以通过阀和旋转阀瓣选择性地连接到清洁回路或与清洁回路断开，以及程序控制系统（11）以及公共部分管路系统。
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5.发明授权SecA gene of mycobacterium tuberculosis and related methods and compositions 失效结核分枝杆菌SecA基因及相关方法及组成
公开(公告)号：US5885828A
公开(公告)日：1999-03-23
申请号：US801105
申请日：1997-02-14
摘要： An isolated nucleic acid encoding a SecA protein of M. tuberculosis is provided. This nucleic acid can be a native coding sequence for the SecA protein of M. tuberculosis. A specific example of the isolated nucleic acid is one that encodes a polypeptide comprising the amino acid sequence set forth in SEQ ID NO:2. An isolated fragment of the secA gene that is specific for M. tuberculosis is provided. A purified SecA protein of Mycobacterium tuberculosis is provided. The purified SecA protein of Mycobacterium tuberculosis comprises the polypeptide having the sequence set forth in the Sequence Listing as SEQ ID NO:2. Fragments of the M. tuberculosis SecA protein are provided. A purified mutant SecA protein of Mycobacterium tuberculosis is provided. The invention provides purified mutant M. tuberculosis expressing the mutant SecA protein of the invention. The invention also provides methods of screening for putative M. tuberculosis virulence factors translocated by the SecA protein.
摘要翻译： 提供编码结核分枝杆菌SecA蛋白的分离核酸。 该核酸可以是结核分枝杆菌SecA蛋白的天然编码序列。 分离的核酸的具体实例是编码包含SEQ ID NO：2所示的氨基酸序列的多肽的核酸的具体实例。 提供了特异于结核分枝杆菌的secA基因的分离片段。 提供了结核分枝杆菌的纯化SecA蛋白。 结核分枝杆菌的纯化SecA蛋白质包含具有序列表所示序列的多肽，如SEQ ID NO：2所示。 提供了结核分枝杆菌SecA蛋白的片段。 提供了结核分枝杆菌的纯化突变体SecA蛋白。 本发明提供了表达本发明的突变体SecA蛋白的纯化的突变体结核分枝杆菌。 本发明还提供了筛选由SecA蛋白转运的推定的结核分枝杆菌毒力因子的方法。
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