公开(公告)号：US07575918B2

公开(公告)日：2009-08-18

申请号：US10935962

申请日：2004-09-08

申请人： James Norris ,  Gary Clawson ,  Caroline Westwater ,  David Schofield ,  Michael G. Schmidt ,  Brian Hoel ,  Joseph Dolan ,  Wei-Hua Pan

发明人： James Norris ,  Gary Clawson ,  Caroline Westwater ,  David Schofield ,  Michael G. Schmidt ,  Brian Hoel ,  Joseph Dolan ,  Wei-Hua Pan

IPC分类号： C07H21/04 ,  C07H21/02 ,  C12Q1/68 ,  A61K31/70

CPC分类号： C08K5/20 ,  A61K38/00 ,  A61K48/00 ,  C08K5/29 ,  C12N15/113 ,  C12N15/74 ,  C12N2310/111 ,  C12N2310/12 ,  C12N2310/317 ,  C12N2799/021 ,  C08L69/00

摘要： The present invention relates to the discovery, identification and characterization of toxic agents which are lethal to pathogens and methods for targeting such toxic agents to a pathogen or pathogen infected cells in order to treat and/or eradicate the infection. In particular, the present invention relates to toxic agents which target bacteria at different stages of the bacterial life cycle, which are delivered alone or in combination to bacteria or bacteria-infected cells. The invention relates to toxic agents which are lethal to diseased cells and methods for targeting such toxic agents to a diseased cell in order to treat and/or eradicate the disease. The present invention relates to promoter elements which are pathogen-specific or tissue-specific and the use of such promoter elements to achieve pathogen-specific or tissue-specific expression of the toxic agent(s) and/or ribozyme(s) of the present invention. Specifically, the invention relates to the delivery of one or more toxic gene products, antisense RNAs, or ribozymes, or combination thereof. The invention provides a novel system by which multiple pathogenic targets may be simultaneously targeted to cause the death of a pathogen, or cell infected with a pathogen. Further, the invention has important implications in the eradication of drug-resistant bacterium and bacterial pathogens. The invention provides a novel system by which multiple targets may be simultaneously targeted to cause the death of a diseased cell. The invention has important implications in the eradication of drug-resistant pathogens (such as antibiotic resistant bacteria) and drug-resistant diseased cells (such as drug-resistant cancer cells).

摘要翻译： 本发明涉及对病原体致死的毒性试剂的发现，鉴定和表征以及将这些毒性试剂靶向病原体或病原体感染的细胞以便治疗和/或根除感染的方法。 特别地，本发明涉及针对细菌生命周期不同阶段的细菌的毒性试剂，它们单独或组合递送至细菌或细菌感染细胞。 本发明涉及对患病细胞致死的毒物剂和用于将这些毒物剂靶向病变细胞以便治疗和/或根除疾病的方法。 本发明涉及作为病原体特异性或组织特异性的启动子元件以及这些启动子元件用于实现本发明的毒性剂和/或核酶的病原体特异性或组织特异性表达的启动子元件 发明。 具体地，本发明涉及递送一种或多种有毒基因产物，反义RNA或核酶或其组合。 本发明提供了一种新的系统，通过该系统可以同时靶向多种致病性靶标以引起病原体或感染病原体的细胞的死亡。 此外，本发明对消除耐药细菌和细菌病原体具有重要的意义。 本发明提供了一种新颖的系统，通过该系统可以同时靶向多个靶标以引起患病细胞的死亡。 本发明对消除耐药性病原体（如抗生素抗性细菌）和耐药性疾病细胞（如耐药性癌细胞）具有重要意义。

公开(公告)号：US06271359B1

公开(公告)日：2001-08-07

申请号：US09291902

申请日：1999-04-14

申请人： James Norris ,  Gary Clawson ,  Caroline Westwater ,  David Schofield ,  Michael Schmidt ,  Brian Hoel ,  Joseph Dolan ,  Wei-Hua Pan

发明人： James Norris ,  Gary Clawson ,  Caroline Westwater ,  David Schofield ,  Michael Schmidt ,  Brian Hoel ,  Joseph Dolan ,  Wei-Hua Pan

IPC分类号： C07H2102

CPC分类号： C12N15/113 ,  A61K38/00 ,  A61K48/00 ,  C12N15/74 ,  C12N2310/111 ,  C12N2310/12 ,  C12N2799/021

摘要： The present invention relates to the discovery, identification and characterization of toxic agents which are lethal to pathogens and methods for targeting such toxic agents to a pathogen or pathogen infected cells in order to treat and/or eradicate the infection. In particular, the present invention relates to toxic agents which target bacteria at different stages of the bacterial life cycle, which are delivered alone or in combination to bacteria or bacteria-infected cells. The invention relates to toxic agents which are lethal to diseased cells and methods for targeting such toxic agents to a diseased cell in order to treat and/or eradicate the disease. The present invention relates to promoter elements which are pathogen-specific or tissue-specific and the use of such promoter elements to achieve pathogen-specific or tissue-specific expression of the toxic agent(s) and/or ribozyme(s) of the present invention. Specifically, the invention relates to the delivery of one or more toxic gene products, antisense RNAs, or ribozymes, or combination thereof. The invention provides a novel system by which multiple pathogenic targets may be simultaneously targeted to cause the death of a pathogen, or cell infected with a pathogen. Further, the invention has important implications in the eradication of drug-resistant bacterium and bacterial pathogens. The invention provides a novel system by which multiple targets may be simultaneously targeted to cause the death of a diseased cell. The invention also has important implications in the eradication of drug-resistant pathogens and drug-resistant diseased cells (such as cancer cells).

摘要翻译： 本发明涉及对病原体致死的毒性试剂的发现，鉴定和表征以及将这些毒性试剂靶向病原体或病原体感染的细胞以便治疗和/或根除感染的方法。 特别地，本发明涉及针对细菌生命周期不同阶段的细菌的毒性试剂，它们单独或组合递送至细菌或细菌感染细胞。 本发明涉及对患病细胞致死的毒物剂和用于将这些毒物剂靶向病变细胞以便治疗和/或根除疾病的方法。 本发明涉及作为病原体特异性或组织特异性的启动子元件以及这些启动子元件用于实现本发明的毒性剂和/或核酶的病原体特异性或组织特异性表达的启动子元件 发明。 具体地，本发明涉及递送一种或多种有毒基因产物，反义RNA或核酶或其组合。 本发明提供了一种新的系统，通过该系统可以同时靶向多种致病性靶标以引起病原体或感染病原体的细胞的死亡。 此外，本发明对消除耐药细菌和细菌病原体具有重要的意义。 本发明提供了一种新颖的系统，通过该系统可以同时靶向多个靶标以引起患病细胞的死亡。 本发明也对消除耐药病原体和耐药性疾病细胞（如癌细胞）具有重要意义。

公开(公告)号：US20050107326A1

公开(公告)日：2005-05-19

申请号：US10935962

申请日：2004-09-08

申请人： James Norris ,  Gary Clawson ,  Caroline Westwater ,  David Schofield ,  Michael Schmidt ,  Brian Hoel ,  Joseph Dolan ,  Wei-Hua Pan

发明人： James Norris ,  Gary Clawson ,  Caroline Westwater ,  David Schofield ,  Michael Schmidt ,  Brian Hoel ,  Joseph Dolan ,  Wei-Hua Pan

IPC分类号： C12N15/09 ,  A61K35/74 ,  A61K35/76 ,  A61K38/00 ,  A61K48/00 ,  A61P31/04 ,  C08K5/20 ,  C08K5/29 ,  C12N7/00 ,  C12N9/00 ,  C12N15/113 ,  C12N15/74 ,  A01K67/00 ,  C07H21/02

CPC分类号： C08K5/20 ,  A61K38/00 ,  A61K48/00 ,  C08K5/29 ,  C12N15/113 ,  C12N15/74 ,  C12N2310/111 ,  C12N2310/12 ,  C12N2310/317 ,  C12N2799/021 ,  C08L69/00

摘要： The present invention relates to the discovery, identification and characterization of toxic agents which are lethal to pathogens and methods for targeting such toxic agents to a pathogen or pathogen infected cells in order to treat and/or eradicate the infection. In particular, the present invention relates to toxic agents which target bacteria at different stages of the bacterial life cycle, which are delivered alone or in combination to bacteria or bacteria-infected cells. The invention relates to toxic agents which are lethal to diseased cells and methods for targeting such toxic agents to a diseased cell in order to treat and/or eradicate the disease. The present invention relates to promoter elements which are pathogen-specific or tissue-specific and the use of such promoter elements to achieve pathogen-specific or tissue-specific expression of the toxic agent(s) and/or ribozyme(s) of the present invention. Specifically, the invention relates to the delivery of one or more toxic gene products, antisense RNAs, or ribozymes, or combination thereof. The invention provides a novel system by which multiple pathogenic targets may be simultaneously targeted to cause the death of a pathogen, or cell infected with a pathogen. Further, the invention has important implications in the eradication of drug-resistant bacterium and bacterial pathogens. The invention provides a novel system by which multiple targets may be simultaneously targeted to cause the death of a diseased cell. The invention has important implications in the eradication of drug-resistant pathogens (such as antibiotic resistant bacteria) and drug-resistant diseased cells (such as drug-resistant cancer cells).

摘要翻译： 本发明涉及对病原体致死的毒性试剂的发现，鉴定和表征以及将这些毒性试剂靶向病原体或病原体感染的细胞以便治疗和/或根除感染的方法。 特别地，本发明涉及针对细菌生命周期不同阶段的细菌的毒性试剂，它们单独或组合递送至细菌或细菌感染细胞。 本发明涉及对患病细胞致死的毒物剂和用于将这些毒物剂靶向病变细胞以便治疗和/或根除疾病的方法。 本发明涉及作为病原体特异性或组织特异性的启动子元件以及这些启动子元件用于实现本发明的毒性剂和/或核酶的病原体特异性或组织特异性表达的启动子元件 发明。 具体地，本发明涉及递送一种或多种有毒基因产物，反义RNA或核酶或其组合。 本发明提供了一种新的系统，通过该系统可以同时靶向多种致病性靶标以引起病原体或感染病原体的细胞的死亡。 此外，本发明对消除耐药细菌和细菌病原体具有重要的意义。 本发明提供了一种新颖的系统，通过该系统可以同时靶向多个靶标以引起患病细胞的死亡。 本发明对消除耐药性病原体（如抗生素抗性细菌）和耐药性疾病细胞（如耐药性癌细胞）具有重要意义。

公开(公告)号：US20060223774A1

公开(公告)日：2006-10-05

申请号：US11375690

申请日：2006-03-13

申请人： James Norris ,  Caroline Westwater ,  David Schofield ,  Michael Schmidt ,  Brian Hoel ,  Joseph Dolan ,  Gary Clawson ,  Wei-Hua Pan

发明人： James Norris ,  Caroline Westwater ,  David Schofield ,  Michael Schmidt ,  Brian Hoel ,  Joseph Dolan ,  Gary Clawson ,  Wei-Hua Pan

IPC分类号： A61K48/00 ,  C07H21/02

CPC分类号： C12N15/1131 ,  A61K38/00 ,  C12N15/113 ,  C12N15/74 ,  C12N2310/111 ,  C12N2310/12 ,  C12N2310/317 ,  C12Q1/6811 ,  C12Q2521/337

摘要： The present invention relates to the discovery, identification and characterization of toxic agents which are lethal to pathogens and methods for targeting such toxic agents to a pathogen or pathogen infected cells in order to treat and/or eradicate the infection. In particular, the present invention relates to toxic agents which target bacteria at different stages of the bacterial life cycle, which are delivered alone or in combination to bacteria or bacteria-infected cells. The invention relates to toxic agents which are lethal to diseased cells and methods for targeting such toxic agents to a diseased cell in order to treat and/or eradicate the disease. The present invention relates to promoter elements which are pathogen-specific or tissue-specific and the use of such promoter elements to achieve pathogen-specific or tissue-specific expression of the toxic agent(s) and/or ribozyme(s) of the present invention. Specifically, the invention relates to the delivery of one or more toxic gene products, antisense RNAs, or ribozymes, or combination thereof. The invention provides a novel system by which multiple pathogenic targets may be simultaneously targeted to cause the death of a pathogen, or cell infected with a pathogen. Further, the invention has important implications in the eradication of drug-resistant bacterium and bacterial pathogens. The invention provides a novel system by which multiple targets may be simultaneously targeted to cause the death of a diseased cell. The invention has important implications in the eradication of drug-resistant pathogens (such as antibiotic resistant bacteria) and drug-resistant diseased cells (such as drug-resistant cancer cells).

摘要翻译： 本发明涉及对病原体致死的毒性试剂的发现，鉴定和表征以及将这些毒性试剂靶向病原体或病原体感染的细胞以便治疗和/或根除感染的方法。 特别地，本发明涉及针对细菌生命周期不同阶段的细菌的毒性试剂，它们单独或组合递送至细菌或细菌感染细胞。 本发明涉及对患病细胞致死的毒物剂和用于将这些毒物剂靶向病变细胞以便治疗和/或根除疾病的方法。 本发明涉及作为病原体特异性或组织特异性的启动子元件以及这些启动子元件用于实现本发明的毒性剂和/或核酶的病原体特异性或组织特异性表达的启动子元件 发明。 具体地，本发明涉及递送一种或多种有毒基因产物，反义RNA或核酶或其组合。 本发明提供了一种新的系统，通过该系统可以同时靶向多种致病性靶标以引起病原体或感染病原体的细胞的死亡。 此外，本发明对消除耐药细菌和细菌病原体具有重要的意义。 本发明提供了一种新颖的系统，通过该系统可以同时靶向多个靶标以引起患病细胞的死亡。 本发明对消除耐药性病原体（如抗生素抗性细菌）和耐药性疾病细胞（如耐药性癌细胞）具有重要意义。

公开(公告)号：US20050118719A1

公开(公告)日：2005-06-02

申请号：US10494880

申请日：2002-11-07

申请人： Michael Schmidt ,  David Schofield ,  Caroline Westwater ,  Joseph Dolan ,  Brian Hoel ,  Philip Werner ,  James Norris ,  Laura Kasman

发明人： Michael Schmidt ,  David Schofield ,  Caroline Westwater ,  Joseph Dolan ,  Brian Hoel ,  Philip Werner ,  James Norris ,  Laura Kasman

IPC分类号： C07K14/01 ,  C12N1/21 ,  C12N15/63 ,  C12N15/73 ,  C12N15/74 ,  C07H21/04

CPC分类号： C12N15/74 ,  C07K14/005 ,  C12N15/63 ,  C12N15/635 ,  C12N15/73 ,  C12N2795/10122

摘要： The invention provides methods and materials involved in delivering nucleic acid to cells and regulating expression of nucleic acid in cells.

摘要翻译： 本发明提供了将核酸递送至细胞并调节核酸在细胞中的表达所涉及的方法和材料。