Transformable inbred corn line LIZL5 and methods for use thereof
    1.
    发明授权
    Transformable inbred corn line LIZL5 and methods for use thereof 有权
    可变形近交玉米线LIZL5及其使用方法

    公开(公告)号:US07205459B1

    公开(公告)日:2007-04-17

    申请号:US09606808

    申请日:2000-06-28

    摘要: According to the invention, there is provided an inbred corn plant designated LIZL5. This invention thus relates to the plants, seeds and tissue cultures of the inbred corn plant LIZL5, and to methods for producing a corn plant produced by crossing the inbred corn plant LIZL5 with itself or with another corn plant, such as another inbred. This invention further relates to corn seeds and plants produced by crossing the inbred plant LIZL5 with another corn plant, such as another inbred, and to crosses with related species. This invention further relates to the inbred and hybrid genetic complements of the inbred corn plant LIZL5, and also to the SSR and genetic isozyme typing profiles of inbred corn plant LIZL5. This invention further relates to methods of transforming the inbred LIZL5 and cells thereof, transformed plants produced by these methods, progeny transgenic plants, and seed derived therefrom.

    摘要翻译: 根据本发明,提供了称为LIZL5的近交玉米植物。 因此,本发明涉及近交玉米植物LIZL5的植物,种子和组织培养物,以及通过将近交玉米植物LIZL5与其他玉米植物如另一近交系交叉产生的玉米植物的生产方法。 本发明还涉及通过将近交植物LIZL5与另一玉米植物(例如另一近交系)杂交并与相关物种杂交而产生的玉米种子和植物。 本发明还涉及近交玉米植物LIZL5的近交和杂交遗传互补,以及近交玉米植物LIZL5的SSR和遗传同工酶分型。 本发明还涉及转化近交LIZL5及其细胞,通过这些方法产生的转化植物,后代转基因植物和从其衍生的种子的方法。

    Method for reduction of transgene copy number
    8.
    发明授权
    Method for reduction of transgene copy number 失效
    降低转基因拷贝数的方法

    公开(公告)号:US6153811A

    公开(公告)日:2000-11-28

    申请号:US995451

    申请日:1997-12-22

    CPC分类号: C12N15/8206 C12N15/8207

    摘要: The present invention provides methods for the efficient production of transformants with low transgene copy numbers. In the method, the average transgene copy number of transformants is decreased through methods which are believed to limit the interaction between segments of transforming DNA prior to transformation. The methods comprise means for end-modification of transforming DNA and use of limited quantities of DNA for transforrnation. Production of single or low copy transformation events is desirable in that it avoids many of the problems associated with high transgene copy number including co-suppression, unpredictable gene expression and non-Mendelian inheritance.

    摘要翻译: 本发明提供了有效生产具有低转基因拷贝数的转化体的方法。 在该方法中,转化体的平均转基因拷贝数通过被认为限制转化前转化DNA片段之间相互作用的方法而降低。 该方法包括用于转化DNA的末端修饰和使用有限量的DNA进行转化的方法。 生产单拷贝或低拷贝转化事件是理想的,因为它避免了与高转基因拷贝数相关的许多问题,包括共抑制,不可预测的基因表达和非孟德尔遗传。

    Fertile transgenic maize plants containing a gene encoding the pat protein
    9.
    发明授权
    Fertile transgenic maize plants containing a gene encoding the pat protein 失效
    含有编码pat蛋白的基因的可育转基因玉米植物

    公开(公告)号:US06395966B1

    公开(公告)日:2002-05-28

    申请号:US08357497

    申请日:1994-12-16

    IPC分类号: A01H500

    摘要: The present invention provides methods for increasing yield in plants by introducing a gene encoding phosphinothricin acetyltransferase. The invention further involves a method of transferring said increased yield phenotype to other lines of plants by crossing. A maize transformant is identified in which the phosphinothricin acetyltransferase gene integration event is correlated with increased yield.

    摘要翻译: 本发明提供了通过引入编码膦丝菌素乙酰转移酶的基因来提高植物产量的方法。 本发明还涉及通过杂交将所述增加的产量表型转移到其它植物系的方法。 鉴定出一种玉米转化体,其中膦丝菌素乙酰转移酶基因整合事件与产量增加相关。

    Methods and compositions for the production of stably transformed,
fertile monocot plants and cells thereof
    10.
    发明授权
    Methods and compositions for the production of stably transformed, fertile monocot plants and cells thereof 失效
    用于生产稳定转化的肥沃单子叶植物及其细胞的方法和组合物

    公开(公告)号:US6025545A

    公开(公告)日:2000-02-15

    申请号:US440689

    申请日:1995-05-15

    摘要: This invention relates to a reproducible system for the production of stable, genetically transformed maize cells, and to methods of selecting cells that have been transformed. One method of selection disclosed employs the Streptomyces bar gene introduced by microprojectile bombardment into embryogenic maize cells which were grown in suspension cultures, followed by exposure to the herbicide bialaphos. The methods of achieving stables transformation disclosed herein include tissue culture methods and media, methods for the bombardment of recipient cells with the desired transforming DNA, and methods of growing fertile plants from the transformed cells. This invention also relates to the transformed cells and seeds and to the fertile plants grown from the transformed cells and to their pollen.

    摘要翻译: 本发明涉及用于生产稳定的遗传转化的玉米细胞的可再现系统,以及选择已经转化的细胞的方法。 公开的一种选择方法使用通过微粒轰击引入的链霉菌棒基因,其在悬浮培养物中生长,然后暴露于除草剂双乙二胺磷的胚发生玉米细胞中。 本文公开的实现马厩转化的方法包括组织培养方法和培养基,用所需转化DNA轰击受体细胞的方法,以及从转化细胞生长可育植物的方法。 本发明还涉及转化的细胞和种子以及从转化细胞和其花粉生长的可育植物。