公开(公告)号：US5489520A

公开(公告)日：1996-02-06

申请号：US233067

申请日：1994-04-26

申请人： Thomas R. Adams ,  Sheryl A. Chambers ,  Richard J. Daines ,  William J. Gordon-Kamm ,  Albert P. Kausch ,  Peggy G. Lemaux ,  Catherine J. Mackey ,  Mary L. Mangano ,  James V. O'Brien ,  Thomas B. Rice ,  T. Michael Spencer ,  William G. Start ,  Nancy G. Willetts

发明人： Thomas R. Adams ,  Sheryl A. Chambers ,  Richard J. Daines ,  William J. Gordon-Kamm ,  Albert P. Kausch ,  Peggy G. Lemaux ,  Catherine J. Mackey ,  Mary L. Mangano ,  James V. O'Brien ,  Thomas B. Rice ,  T. Michael Spencer ,  William G. Start ,  Nancy G. Willetts

IPC分类号： A01H4/00 ,  C07K14/415 ,  C12N5/00 ,  C12N5/04 ,  C12N15/05 ,  C12N15/82 ,  C12N15/87

CPC分类号： A01H4/008 ,  A01H4/00 ,  A01H4/001 ,  C07K14/415 ,  C12N15/8207 ,  C12N15/87 ,  C12N5/0025 ,  C12N5/04 ,  Y10S47/01

摘要： This invention relates to a reproducible system for the production of stable, genetically transformed maize cells, and to methods of selecting cells that have been transformed. One method of selection disclosed employs the Streptomyces bar gene introduced by microprojectile bombardment into embryogenic maize cells which were grown in suspension cultures, followed by exposure to the herbicide bialaphos. The methods of achieving stable transformation disclosed herein include tissue culture methods and media, methods for the bombardment of recipient cells with the desired transforming DNA, and methods of growing fertile plants from the transformed cells. This invention also relates to the transformed cells and seeds and to the fertile plants grown from the transformed cells and to their pollen.

摘要翻译： 本发明涉及用于生产稳定的遗传转化的玉米细胞的可再现系统，以及选择已经转化的细胞的方法。 公开的一种选择方法使用通过微粒轰击引入的链霉菌棒基因，其在悬浮培养物中生长，然后暴露于除草剂双乙二胺磷的胚发生玉米细胞中。 实现本文公开的稳定转化的方法包括组织培养方法和培养基，用所需转化DNA轰击受体细胞的方法，以及从转化细胞生长可育植物的方法。 本发明还涉及转化的细胞和种子以及从转化细胞和其花粉生长的可育植物。

公开(公告)号：US5919675A

公开(公告)日：1999-07-06

申请号：US446933

申请日：1995-05-23

申请人： Thomas R. Adams ,  Sheryl A. Chambers ,  Richard J. Daines ,  William J. Gordon-Kamm ,  Albert P. Kausch ,  Peggy G. Lemaux ,  Catherine J. Mackey ,  Mary L. Mangano ,  James V. O'Brien ,  Thomas B. Rice ,  T. Michael Spencer ,  William G. Start ,  Nancy G. Willetts

发明人： Thomas R. Adams ,  Sheryl A. Chambers ,  Richard J. Daines ,  William J. Gordon-Kamm ,  Albert P. Kausch ,  Peggy G. Lemaux ,  Catherine J. Mackey ,  Mary L. Mangano ,  James V. O'Brien ,  Thomas B. Rice ,  T. Michael Spencer ,  William G. Start ,  Nancy G. Willetts

IPC分类号： A01H4/00 ,  C07K14/415 ,  C12N5/00 ,  C12N5/04 ,  C12N15/05 ,  C12N15/82 ,  C12N15/87 ,  C12N15/00 ,  A01H1/06

CPC分类号： A01H4/008 ,  A01H4/00 ,  A01H4/001 ,  C07K14/415 ,  C12N15/8207 ,  C12N15/87 ,  C12N5/0025 ,  C12N5/04 ,  Y10S47/01

摘要： This invention relates to a method for the production of stable, genetically transformed, Zea mays plants that comprise an exogenous DNA expressing phosphinothricin acetyl transferase to impart resistance to phosphinothricin in said plants. The method further comprises transforming said Zea mays plants with a second gene and further still wherein said second gene imparts insect resistance, such as the expression of a Bacillus thuringiensis toxin gene. The method for achieving stable transformation includes tissue culture methods and media, methods for the bombardment of recipient cells with said DNA's, and methods of regenerating fertile plants from transformed cells. The invention also pertains to a method of breeding said transgenic Zea mays plants with a either a non-transgenic plant or a transgenic plant, and the obtention of fertile transgenic plants and seed therefrom.

摘要翻译： 本发明涉及一种用于生产稳定的转基因玉米植物的方法，其包含表达草甘膦乙酰转移酶的外源DNA，以赋予所述植物中的次氯氰菊酯抗性。 该方法还包括用第二基因转化所述玉米植物，并且还进一步其中所述第二基因赋予昆虫抗性，例如苏云金芽孢杆菌毒素基因的表达。 实现稳定转化的方法包括组织培养方法和培养基，用所述DNA轰击受体细胞的方法，以及从转化细胞再生可育植物的方法。 本发明还涉及用非转基因植物或转基因植物育种所述转基因玉米植物的方法，以及获得可育转基因植物和种子。

公开(公告)号：US5874265A

公开(公告)日：1999-02-23

申请号：US446931

申请日：1995-05-23

申请人： Thomas R. Adams ,  Sheryl A. Chambers ,  Richard J. Daines ,  William J. Gordon-Kamm ,  Albert P. Kausch ,  Peggy G. Lemaux ,  Catherine J. Mackey ,  Mary L. Mangano ,  James V. O'Brien ,  Thomas B. Rice ,  T. Michael Spencer ,  William G. Start ,  Nancy G. Willetts

发明人： Thomas R. Adams ,  Sheryl A. Chambers ,  Richard J. Daines ,  William J. Gordon-Kamm ,  Albert P. Kausch ,  Peggy G. Lemaux ,  Catherine J. Mackey ,  Mary L. Mangano ,  James V. O'Brien ,  Thomas B. Rice ,  T. Michael Spencer ,  William G. Start ,  Nancy G. Willetts

IPC分类号： A01H4/00 ,  C07K14/415 ,  C12N5/00 ,  C12N5/04 ,  C12N15/05 ,  C12N15/82 ,  C12N15/87 ,  C12N15/00 ,  A01H1/06

CPC分类号： A01H4/008 ,  A01H4/00 ,  A01H4/001 ,  C07K14/415 ,  C12N15/8207 ,  C12N15/87 ,  C12N5/0025 ,  C12N5/04 ,  Y10S47/01

摘要： This invention relates to a method for the production of stable, genetically transformed cereal plants, other than maize or rice, that comprise an exogenous DNA expressing phosphinothricin acetyl transferase to impart resistance to phosphinothricin in said cereal plants. Cereal plants within the scope of the method include, but are not limited to, wheat, barley and oats. The invention also pertains to a method of breeding said transgenic cereal plants with a either a non-transgenic plant or a transgenic plant, and the obtention of fertile transgenic plants and seed therefrom.

摘要翻译： 本发明涉及一种生产稳定的基因转化谷物植物（玉米或水稻以外）的方法，其包含表达草甘膦乙酰转移酶的外源DNA，以赋予所述谷类植物中的次氯氰菊酯抗性。 在该方法范围内的谷类植物包括但不限于小麦，大麦和燕麦。 本发明还涉及用非转基因植物或转基因植物育种所述转基因谷物植物的方法，以及获得可育转基因植物和种子。

公开(公告)号：US5550318A

公开(公告)日：1996-08-27

申请号：US565844

申请日：1990-08-09

申请人： Thomas R. Adams ,  Sheryl A. Chambers ,  Richard J. Daines ,  William J. Gordon-Kamm ,  Albert P. Kausch ,  Peggy G. Lemaux ,  Catherine J. Mackey ,  Mary L. Mangano ,  James V. O'Brien ,  Thomas B. Rice ,  T. Michael Spencer ,  William G. Start ,  Nancy G. Willetts

发明人： Thomas R. Adams ,  Sheryl A. Chambers ,  Richard J. Daines ,  William J. Gordon-Kamm ,  Albert P. Kausch ,  Peggy G. Lemaux ,  Catherine J. Mackey ,  Mary L. Mangano ,  James V. O'Brien ,  Thomas B. Rice ,  T. Michael Spencer ,  William G. Start ,  Nancy G. Willetts

IPC分类号： A01H4/00 ,  C07K14/415 ,  C12N5/00 ,  C12N5/04 ,  C12N15/05 ,  C12N15/82 ,  C12N15/87 ,  C12N15/00 ,  A01H1/06

CPC分类号： A01H4/008 ,  A01H4/00 ,  A01H4/001 ,  C07K14/415 ,  C12N15/8207 ,  C12N15/87 ,  C12N5/0025 ,  C12N5/04 ,  Y10S47/01

摘要： This invention relates to a reproducible system for the production of stable, genetically transformed maize cells, and to methods of selecting cells that have been transformed. One method of selection disclosed employs the Streptomyces bar gene introduced by microprojectile bombardment into embryogenic maize cells which were grown in suspension cultures, followed by exposure to the herbicide bialaphos. The methods of achieving stable transformation disclosed herein include tissue culture methods and media, methods for the bombardment of recipient cells with the desired transforming DNA, and methods of growing fertile plants from the transformed cells. This invention also relates to the transformed cells and seeds and to the fertile plants grown from the transformed cells and to their pollen.

公开(公告)号：US5969213A

公开(公告)日：1999-10-19

申请号：US446930

申请日：1995-05-23

申请人： Thomas R. Adams ,  Sheryl A. Chambers ,  Richard J. Daines ,  William J. Gordon-Kamm ,  Albert P. Kausch ,  Peggy G. Lemaux ,  Catherine J. Mackey ,  May L. Mangano ,  James V. O'Brien ,  Thomas B. Rice ,  T. Michael Spencer ,  William G. Start ,  Nancy G. Willetts

发明人： Thomas R. Adams ,  Sheryl A. Chambers ,  Richard J. Daines ,  William J. Gordon-Kamm ,  Albert P. Kausch ,  Peggy G. Lemaux ,  Catherine J. Mackey ,  May L. Mangano ,  James V. O'Brien ,  Thomas B. Rice ,  T. Michael Spencer ,  William G. Start ,  Nancy G. Willetts

IPC分类号： A01H4/00 ,  C07K14/415 ,  C12N5/00 ,  C12N5/04 ,  C12N15/05 ,  C12N15/82 ,  C12N15/87 ,  C12N15/00 ,  A01H1/06

CPC分类号： A01H4/008 ,  A01H4/00 ,  A01H4/001 ,  C07K14/415 ,  C12N15/8207 ,  C12N15/87 ,  C12N5/0025 ,  C12N5/04 ,  Y10S47/01

摘要： This invention relates to stable and genetically transformed cereal plants, other than maize or rice, that comprise an exogenous DNA expressing phosphinothricin acetyl transferase to impart resistance to phosphinothricin in said cereal plants. Cereal plants within the scope of the invention include, but are not limited to, wheat, barley and oats. The invention also pertains to transgenic cereal plants which further comprise screenable markers and transgenic seed obtained from said plants.

摘要翻译： 本发明涉及除了玉米或水稻之外的稳定和遗传转化的谷类植物，其包含表达膦丝菌素乙酰转移酶的外源DNA，以在所述谷类植物中赋予对于草丁膦的抗性。 本发明范围内的谷类植物包括但不限于小麦，大麦和燕麦。 本发明还涉及进一步包含从所述植物获得的可筛选标记和转基因种子的转基因谷物植物。

公开(公告)号：US07705215B1

公开(公告)日：2010-04-27

申请号：US08113561

申请日：1993-08-25

申请人： Thomas R. Adams ,  Paul C. Anderson ,  Sheryl A. Chambers ,  Paul S. Chomet ,  Richard J. Daines ,  Christopher E. Flick ,  Kimberly Glassman ,  William J. Gordon-Kamm ,  Albert P. Kausch ,  Lucille B. Laccetti ,  Peggy G. Lemaux ,  Catherine J. Mackey ,  Mary Lou Mangano ,  Michael T. Mann ,  James V. O'Brien ,  Emil M. Orozco ,  Peter Orr ,  Thomas B. Rice ,  T. Michael Spencer ,  William G. Start ,  Michael A. Stephens ,  Clayton S. Vetsch ,  David A. Walters ,  Donald S. Walters ,  Nancy G. Willetts ,  Susan J. Zachwieja

发明人： Thomas R. Adams ,  Paul C. Anderson ,  Sheryl A. Chambers ,  Paul S. Chomet ,  Richard J. Daines ,  Christopher E. Flick ,  Kimberly Glassman ,  William J. Gordon-Kamm ,  Albert P. Kausch ,  Lucille B. Laccetti ,  Peggy G. Lemaux ,  Catherine J. Mackey ,  Mary Lou Mangano ,  Michael T. Mann ,  James V. O'Brien ,  Emil M. Orozco ,  Peter Orr ,  Thomas B. Rice ,  T. Michael Spencer ,  William G. Start ,  Michael A. Stephens ,  Clayton S. Vetsch ,  David A. Walters ,  Donald S. Walters ,  Nancy G. Willetts ,  Susan J. Zachwieja

IPC分类号： C12N15/82 ,  C12N5/04 ,  A01H5/00 ,  A01H5/10 ,  C12N15/53 ,  C12P7/64

CPC分类号： C12N15/8241 ,  A01H4/00 ,  A01H4/001 ,  C07K14/415 ,  C12N5/0025 ,  C12N5/04 ,  C12N9/0006 ,  C12N9/88 ,  C12N15/8207 ,  C12N15/8209 ,  C12N15/87

摘要： This invention relates to a reproducible system for the production of stable, genetically transformed maize cells, and to methods of selecting cells that have been transformed. One method of selection disclosed employs the Streptomyces bar gene introduced by microprojectile bombardment into embryogenic maize cells which were grown in suspension cultures, followed by exposure to the herbicide bialaphos. The methods of achieving stable transformation disclosed herein include tissue culture methods and media, methods for the bombardment of recipient cells with the desired transforming DNA, and methods of growing fertile plants from the transformed cells. This invention also relates to the transformed cells and seeds and to the fertile plants grown from the transformed cells and to their pollen.

摘要翻译： 本发明涉及用于生产稳定的遗传转化的玉米细胞的可再现系统，以及选择已经转化的细胞的方法。 公开的一种选择方法使用通过微粒轰击引入的链霉菌棒基因，其在悬浮培养物中生长，然后暴露于除草剂双乙二胺磷的胚发生玉米细胞中。 实现本文公开的稳定转化的方法包括组织培养方法和培养基，用所需转化DNA轰击受体细胞的方法，以及从转化细胞生长可育植物的方法。 本发明还涉及转化的细胞和种子以及从转化细胞和其花粉生长的可育植物。

公开(公告)号：US06281411B1

公开(公告)日：2001-08-28

申请号：US08599714

申请日：1996-01-19

申请人： Thomas R. Adams ,  Paul C. Anderson ,  Richard J. Daines ,  William Gordon-Kamm ,  Albert P. Kausch ,  Michael T. Mann ,  Peter M. Orr ,  David C. Warner

发明人： Thomas R. Adams ,  Paul C. Anderson ,  Richard J. Daines ,  William Gordon-Kamm ,  Albert P. Kausch ,  Michael T. Mann ,  Peter M. Orr ,  David C. Warner

IPC分类号： A01H106

CPC分类号： C12N9/88 ,  C12N9/0006 ,  C12N15/8207 ,  C12N15/8209 ,  C12N15/8241

摘要： The present invention provides a method for conferring tolerance or resistance to water or salt stress in a monocot plant, and/or altering the osmoprotectant content of a monocot plant, by introducing a preselected DNA segment into the plant. This invention also relates to the transformed cells and seeds, and to the fertile plants grown from the transformed cells and to their pollen.

摘要翻译： 本发明提供了通过将预先选择的DNA片段引入植物中来赋予单子叶植物中耐水性或耐盐性胁迫的方法和/或改变单子叶植物的渗透保护剂含量。 本发明还涉及转化的细胞和种子以及从转化细胞和其花粉生长的可育植物。

公开(公告)号：US5780709A

公开(公告)日：1998-07-14

申请号：US594861

申请日：1996-01-19

申请人： Thomas R. Adams ,  Paul C. Anderson ,  Richard J. Daines ,  William Gordon-Kamm ,  Albert P. Kausch ,  Michael T. Mann ,  Peter M. Orr ,  David C. Warner

发明人： Thomas R. Adams ,  Paul C. Anderson ,  Richard J. Daines ,  William Gordon-Kamm ,  Albert P. Kausch ,  Michael T. Mann ,  Peter M. Orr ,  David C. Warner

IPC分类号： C12N9/04 ,  C12N9/88 ,  C12N15/29 ,  C12N15/82 ,  A01H5/00 ,  C12N15/00 ,  C12N15/05

CPC分类号： C12N9/0006 ,  C12N15/8209 ,  C12N15/8241 ,  C12N15/8245 ,  C12N15/8273 ,  C12N9/88 ,  Y10S47/01

摘要： The present invention provides a method for conferring tolerance or resistance to water or salt stress in a monocot plant, and/or altering the osmoprotectant content of a monocot plant, by introducing a preselected DNA segment into the plant. This invention also relates to the transformed cells and seeds, and to the fertile plants grown from the transformed cells and to their pollen.

摘要翻译： 本发明提供了通过将预先选择的DNA片段引入植物中来赋予单子叶植物中耐水性或耐盐性胁迫的方法和/或改变单子叶植物的渗透保护剂含量。 本发明还涉及转化的细胞和种子以及从转化细胞和其花粉生长的可育植物。

公开(公告)号：US20090049573A1

公开(公告)日：2009-02-19

申请号：US11982274

申请日：2007-10-31

申请人： Stanton B. Dotson ,  Michael D. Edgerton ,  Jingrui Wu ,  Zhidong Xie ,  Garrett J. Lee ,  Thomas R. Adams ,  Donald E. Nelson

发明人： Stanton B. Dotson ,  Michael D. Edgerton ,  Jingrui Wu ,  Zhidong Xie ,  Garrett J. Lee ,  Thomas R. Adams ,  Donald E. Nelson

IPC分类号： C12N15/29 ,  A01H5/00 ,  C12N15/11

CPC分类号： C12N15/8273 ,  C12N15/8261 ,  Y02A40/146

摘要： This invention provides transgenic plant cells with recombinant DNA for expression of proteins that are useful for imparting enhanced agronomic trait(s) to transgenic crop plants. This invention also provides transgenic plants and progeny seed comprising the transgenic plant cells where the plants are selected for having an enhanced trait selected from the group of traits consisting of enhanced water use efficiency, enhanced cold tolerance, increased yield, enhanced nitrogen use efficiency, enhanced seed protein and enhanced seed oil. Also disclosed are methods for manufacturing transgenic seed and plants with enhanced traits.

公开(公告)号：US07704067B2

公开(公告)日：2010-04-27

申请号：US11348483

申请日：2006-02-07

申请人： Thomas R. Adams ,  Gary R. Wittman

发明人： Thomas R. Adams ,  Gary R. Wittman

IPC分类号： B29C43/18

CPC分类号： B29C43/36 ,  B29C33/30 ,  B29C43/027 ,  B29C70/462 ,  B29K2105/08 ,  B29L2031/3076

摘要： A mold and a method for molding high strength, non-metallic fiber reinforced composite parts having a variety of (e.g., cylindrical) shapes that are lighter in weight than similar parts manufactured from metal. An unmolded non-metallic pre-form having long continuous fibers is placed around a mandrel, and a segmented, adjustable diameter compression ring is disposed in surrounding engagement with the pre-form at an upper position within an inwardly tapered compression ring support. The compression ring is formed from a plurality of loose compression ring segments that are initially spaced circumferentially from one another at the upper position of the compression ring support. The mandrel and the pre-form are first heated and then located in a press. The press is closed to push the compression ring and the heated mandrel from the upper position of the compression ring support, at which the compression ring has an open configuration and a wide diameter, to a lower position, at which the compression ring has a closed configuration and a narrow diameter. As the compression ring is pushed to the lower position, the compression ring segments slide along the inwardly tapered compression ring support so as to be moved end-to-end one another to apply a compressive force for consolidating and shaping the heated pre-form into the molded part.

摘要翻译： 一种用于模制高强度，非金属纤维增强复合材料的模具和方法，其具有比由金属制成的类似零件重量更轻的各种（例如，圆柱形）形状。 具有长连续纤维的未模塑的非金属预成型件放置在心轴周围，并且分段的可调节直径的压缩环设置成与内部锥形压缩环支撑件中的上部位置与预成型件周围接合。 压缩环由多个松散的压缩环段形成，该压缩环段最初在压缩环支撑件的上部位置处彼此周向地间隔开。 心轴和预成型件首先加热然后位于压机中。 压力机关闭以将压缩环和加热的心轴从压缩环支撑件的上部位置（压缩环具有打开构造和宽直径）推动到下部位置，压力环在该位置处具有闭合 配置和狭窄的直径。 当压缩环被推到下部位置时，压缩环段沿向内锥形的压缩环支撑件滑动，以便彼此端对端地移动以施加压缩力，以将加热的预成型件固结和成形为 模制件。