公开(公告)号：US08338128B2

公开(公告)日：2012-12-25

申请号：US12749814

申请日：2010-03-30

申请人： Jason Lye ,  John Gavin MacDonald ,  Ning Wei ,  Stephanie M. Martin

发明人： Jason Lye ,  John Gavin MacDonald ,  Ning Wei ,  Stephanie M. Martin

IPC分类号： C12Q1/22 ,  C12Q1/02

CPC分类号： C12Q1/04

摘要： Suitable dyes are used herein to indicate the presence of microbial contamination by spraying them onto surfaces in the form of an aqueous solution. The dye solution may also be allowed to dry, thus producing the dried residue of an aqueous solution. It's believed that these dyes change color in response to a change in polarity of the environment. Since water is a polar solvent and most bacteria are made from non-polar substances, the presence of bacteria changes the polarity of the environment, triggering a change visible by the unaided eye.

摘要翻译： 合适的染料在本文中用于通过以水溶液的形式将它们喷雾到表面上来表示存在微生物污染。 染料溶液也可以干燥，从而产生水溶液的干燥残余物。 据信这些染料响应于环境极性的变化而改变颜色。 由于水是极性溶剂，大多数细菌都是由非极性物质制成的，所以细菌的存在会改变环境的极性，引发肉眼可见的变化。

公开(公告)号：US08361742B2

公开(公告)日：2013-01-29

申请号：US12843922

申请日：2010-07-27

申请人： Stephanie M. Martin ,  Andrew M. Long, III ,  John Gavin MacDonald ,  Jason Lye

发明人： Stephanie M. Martin ,  Andrew M. Long, III ,  John Gavin MacDonald ,  Jason Lye

IPC分类号： C12Q1/22 ,  C12Q1/02

CPC分类号： A61L15/56 ,  G01N2333/245 ,  G01N2333/31 ,  G01N2333/40

摘要： A method and system for rapidly detecting Candida on the skin of a host, such as an infant with diaper rash, is provided. The method includes contacting a dermal sample with a colorant that exhibits a certain spectral response (e.g., color change) in the presence of Candida. For example, the colorant may change from a first color to a second color, from colorless to a color, or from a color to colorless. The colorant is typically capable of differentiating between Candida (e.g., Candida albicans) and other microorganisms commonly associated with diaper rash, such as S. aureus and E. coli. Thus, when a dermal sample is placed into contact with the colorant, the color change may simply be observed to determine whether the infection is caused by Candida. If the color change occurs to a certain extent (e.g., from yellow to bright red), it may be determined that the test sample contains Candida. Likewise, if a color change occurs to a lesser extent (e.g., from yellow to faint orange) or not at all, it may be determined that the dermal sample contains other microorganisms (e.g., S. aureus or E. coli), no infection is present, or that the infection is simply due to other causes. Regardless, it will become readily apparent whether or not treatment for Candida is needed.

摘要翻译： 提供了用于快速检测宿主皮肤上的假丝酵母的方法和系统，例如具有尿布疹的婴儿。 该方法包括使真皮样品与在假丝酵母存在下显示特定光谱响应（例如颜色变化）的着色剂接触。 例如，着色剂可以从第一颜色变为第二颜色，从无色到颜色，或从颜色变为无色。 着色剂通常能够区分假丝酵母（例如白色念珠菌）和通常与尿布疹相关的其它微生物，例如金黄色葡萄球菌和大肠杆菌。 因此，当真皮样品与着色剂接触时，可以简单地观察颜色变化以确定感染是否由念珠菌引起。 如果颜色变化发生一定程度（例如，从黄色到鲜红色），则可以确定测试样品含有念珠菌。 同样地，如果在较小程度上发生颜色变化（例如，从黄色到微弱橙色），或根本不发生变色，则可以确定真皮样品含有其他微生物（例如金黄色葡萄球菌或大肠杆菌），无感染 存在，或感染仅仅是由于其他原因。 无论是否需要对念珠菌的治疗，将变得显而易见。

公开(公告)号：US07399608B2

公开(公告)日：2008-07-15

申请号：US11117651

申请日：2005-04-28

申请人： John Gavin MacDonald ,  Stephanie M. Martin ,  Jason Lye

发明人： John Gavin MacDonald ,  Stephanie M. Martin ,  Jason Lye

IPC分类号： C12Q1/02

CPC分类号： C12Q1/06 ,  C12Q1/04 ,  Y10T442/2566

摘要： A method for semi-quantitatively or quantitatively detecting the presence of a microbe in a sample is provided. The method utilizes a test dye that undergoes a detectable color change in the presence of one or more microbes. For example, in one embodiment, the test dye is a solvatochromic dye (e.g., Reichardt's dye) that responds to differences in polarity between microbe components (e.g., cell membrane, cytoplasm, etc.) and the environment outside the cell. Alternatively, other mechanisms may be wholly or partially responsible for the interaction between the dye and the microbe, such as acid-base reactions, redox reactions, and so forth. Regardless, the color of the test dye may be compared to the color of a control dye, wherein the color of the control dye corresponds to a known microbe concentration.

摘要翻译： 提供了一种半定量或定量检测样品中微生物存在的方法。 该方法利用在一种或多种微生物存在下经历可检测的颜色变化的测试染料。 例如，在一个实施方案中，测试染料是响应于微生物组分（例如细胞膜，细胞质等）与细胞外的环境的极性差异的溶剂化色素染料（例如，Reichardt's染料）。 或者，其它机理可以完全或部分地负责染料和微生物之间的相互作用，例如酸碱反应，氧化还原反应等。 无论如何，可以将测试染料的颜色与对照染料的颜色进行比较，其中对照染料的颜色对应于已知的微生物浓度。

公开(公告)号：US07300770B2

公开(公告)日：2007-11-27

申请号：US11117635

申请日：2005-04-28

申请人： Stephanie M. Martin ,  John Gavin MacDonald ,  Allison Salyer Bagwell ,  Jason Lye ,  Robert B. Johnson

发明人： Stephanie M. Martin ,  John Gavin MacDonald ,  Allison Salyer Bagwell ,  Jason Lye ,  Robert B. Johnson

IPC分类号： C12Q1/22

CPC分类号： C12Q1/04 ,  G01N33/528

摘要： An elastomeric article that contains a chromogen that undergoes a detectable change in color in the presence of one or more microbes is provided. For example, in one embodiment, the chromogen is a solvatochromic dye (e.g., Reichardt's dye) that undergoes a color change in the presence of bacteria or other microbes. More specifically, such dyes may respond to differences in polarity between microbe components (e.g., cell membrane, cytoplasm, etc.) and the environment outside the cell. Alternatively, other mechanisms may be wholly or partially responsible for the interaction between the dye and the microbe, such as acid-base reactions, redox reactions, and so forth.

摘要翻译： 提供了包含在一种或多种微生物存在下经历可检测的颜色变化的色原体的弹性制品。 例如，在一个实施方案中，色原是在细菌或其它微生物存在下经历颜色变化的溶剂化色素染料（例如，Reichardt's染料）。 更具体地，这样的染料可以响应微生物组分（例如细胞膜，细胞质等）与细胞外的环境之间的极性差异。 或者，其它机理可以完全或部分地负责染料和微生物之间的相互作用，例如酸碱反应，氧化还原反应等。

公开(公告)号：US07727513B2

公开(公告)日：2010-06-01

申请号：US11303001

申请日：2005-12-15

申请人： John Gavin MacDonald ,  Stephanie M. Martin ,  Jason Lye

发明人： John Gavin MacDonald ,  Stephanie M. Martin ,  Jason Lye

IPC分类号： A61K49/00 ,  C12Q1/20 ,  C12Q1/22 ,  C12Q1/04

CPC分类号： C12Q1/04 ,  C12Q1/14

摘要： A method for rapidly detecting infectious conjunctivitis in a host is provided. The method includes contacting an ocular test sample with a chromogen (e.g., Reichardt's dye) that exhibits a color change in the presence of a microbe. The present inventors have discovered that the extent of the color change may vary depending on whether the microbe is a bacteria or virus. Without intending to be limited by theory, the present inventors believe that the chromogen interacts with the peptidoglycan-based cell wall structure of bacteria to induce a color change that is even more apparent at infectious levels. It is believed that this interaction occurs to a much greater extent in bacteria than in viruses. Accordingly, although the chromogen may still undergo a color change in the presence of the viruses, it is typically to a much lesser extent. In this manner, the degree of color change of the chromogen may be used in the present invention as a mechanism for differentiating between viral and bacterial conjunctivitis.

摘要翻译： 提供了一种快速检测宿主感染性结膜炎的方法。 该方法包括使眼睛测试样品与在微生物存在下显示颜色变化的色原体（例如，Reichardt's染料）接触。 本发明人已经发现，颜色变化的程度可以根据微生物是细菌还是病毒而变化。 不期望受理论的限制，本发明人相信色原体与细菌的基于聚肽聚糖的细胞壁结构相互作用以诱导在感染水平上更加明显的颜色变化。 相信这种相互作用在细菌中比在病毒中更大程度地发生。 因此，虽然染色体在病毒存在的情况下仍可能发生颜色变化，但通常其程度要小得多。 以这种方式，在本发明中可以使用色原体的颜色变化程度作为区分病毒和细菌性结膜炎的机制。

公开(公告)号：US07282349B2

公开(公告)日：2007-10-16

申请号：US10737574

申请日：2003-12-16

申请人： Jason Lye ,  John Gavin MacDonald ,  Ning Wei

发明人： Jason Lye ,  John Gavin MacDonald ,  Ning Wei

IPC分类号： C12Q1/22

CPC分类号： C12Q1/04

摘要： Solvatochromic dyes are used herein to indicate the presence of bacteria by incorporating them into various substrates such as films, woven and nonwoven fabrics, paper towels, coform and airlaid materials and bonded carded webs. These dyes change color in response to a change in polarity of the environment. Since water is a polar solvent and most bacteria are made from non-polar substances, the presence of bacteria changes the polarity of the environment, triggering a change visible by the unaided eye. A lateral flow device incorporating solvatochromic dyes and a method of detecting bacteria are also included.

摘要翻译： 溶剂化色素染料在本文中用于通过将它们并入各种底物如薄膜，机织和非织造织物，纸巾，共成型和气流成网材料和粘合梳理纤维网中来表示细菌的存在。 这些染料响应于环境极性的变化而改变颜色。 由于水是极性溶剂，大多数细菌都是由非极性物质制成的，所以细菌的存在会改变环境的极性，引发肉眼可见的变化。 还包括含有溶剂化色素染料的侧流装置和检测细菌的方法。

公开(公告)号：US20100248299A1

公开(公告)日：2010-09-30

申请号：US12749814

申请日：2010-03-30

申请人： Jason Lye ,  John Gavin MacDonald ,  Ning Wei ,  Stephanie Martin

发明人： Jason Lye ,  John Gavin MacDonald ,  Ning Wei ,  Stephanie Martin

IPC分类号： C12Q1/04

CPC分类号： C12Q1/04

摘要： Suitable dyes are used herein to indicate the presence of microbial contamination by spraying them onto surfaces in the form of an aqueous solution. The dye solution may also be allowed to dry, thus producing the dried residue of an aqueous solution. It's believed that these dyes change color in response to a change in polarity of the environment. Since water is a polar solvent and most bacteria are made from non-polar substances, the presence of bacteria changes the polarity of the environment, triggering a change visible by the unaided eye.

摘要翻译： 合适的染料在本文中用于通过以水溶液的形式将它们喷雾到表面上来表示存在微生物污染。 染料溶液也可以干燥，从而产生水溶液的干燥残余物。 据信这些染料响应于环境极性的变化而改变颜色。 由于水是极性溶剂，大多数细菌都是由非极性物质制成的，所以细菌的存在会改变环境的极性，引发肉眼可见的变化。

公开(公告)号：US07687245B2

公开(公告)日：2010-03-30

申请号：US10581404

申请日：2004-12-16

申请人： Jason Lye ,  John Gavin MacDonald ,  Ning Wei ,  Stephanie Martin

发明人： Jason Lye ,  John Gavin MacDonald ,  Ning Wei ,  Stephanie Martin

IPC分类号： C12Q1/22

CPC分类号： C12Q1/04

摘要： Suitable dyes are used herein to indicate the presence of microbial contamination by spraying them onto surfaces in the form of an aqueous solution. The dye solution may also be allowed to dry, thus producing the dried residue of an aqueous solution. It's believed that these dyes change color in response to a change in polarity of the environment. Since water is a polar solvent and most bacteria are made from non-polar substances, the presence of bacteria changes the polarity of the environment, triggering a change visible by the unaided eye.

摘要翻译： 合适的染料在本文中用于通过以水溶液的形式将它们喷雾到表面上来表示存在微生物污染。 染料溶液也可以干燥，从而产生水溶液的干燥残余物。 据信这些染料响应于环境极性的变化而改变颜色。 由于水是极性溶剂，大多数细菌都是由非极性物质制成的，所以细菌的存在会改变环境的极性，引发肉眼可见的变化。

公开(公告)号：US08617874B2

公开(公告)日：2013-12-31

申请号：US12464149

申请日：2009-05-12

申请人： Stephanie Michelle Martin ,  John Gavin MacDonald ,  Jason Lye ,  Curt Sayre ,  Kimberlee Thompson

发明人： Stephanie Michelle Martin ,  John Gavin MacDonald ,  Jason Lye ,  Curt Sayre ,  Kimberlee Thompson

IPC分类号： C12M1/34

CPC分类号： C12Q1/04

摘要： A method for detecting a microorganism or class of microorganisms is provided. More specifically, the method employs an array that contains a plurality of discrete regions (referred to as “addresses”) spaced apart on a solid support in a predetermined pattern. The addresses are selected so that the array provides a distinct spectral response (e.g., pattern of colors) or “fingerprint” for a particular microorganism or class of microorganisms. For example, the array may provide a certain spectral response in the presence of one microorganism or class of microoryanisms (e.g., gram-negative bacteria), but provide a completely different spectral response in the presence of another microorganism or class of microorganisms (e.g., gram-positive bacteria). Detection of the spectral response provided by the array may thus allow for differentiation between microorganisms.

摘要翻译： 提供了一种用于检测微生物或微生物类别的方法。 更具体地，该方法采用包含在固体支持体上以预定图案间隔开的多个离散区域（称为“地址”）的阵列。 选择地址使得阵列为特定微生物或微生物类提供不同的光谱响应（例如，颜色图案）或“指纹”。 例如，阵列可以在一种微生物或类微生物（例如，革兰氏阴性细菌）的存在下提供特定的光谱响应，但是在另一种微生物或一类微生物的存在下提供完全不同的光谱响应（例如， 革兰氏阳性细菌）。 由阵列提供的光谱响应的检测因此可以允许微生物之间的分化。

公开(公告)号：US07913640B2

公开(公告)日：2011-03-29

申请号：US11983696

申请日：2007-11-09

申请人： John Gavin MacDonald ,  Allison Salyer Bagwell ,  Molly K. Smith ,  Jeffrey E. Fish ,  Jason Lye ,  Brian J. Cuevas ,  Michael Sleva

发明人： John Gavin MacDonald ,  Allison Salyer Bagwell ,  Molly K. Smith ,  Jeffrey E. Fish ,  Jason Lye ,  Brian J. Cuevas ,  Michael Sleva

IPC分类号： A62B7/10

CPC分类号： A61M16/1045 ,  A61M16/161 ,  A61M2205/584

摘要： There is provided a moisture indicator for a heat and moisture exchange (HME) device that uses a pre-colorant and an activator applied to an inner surface of the HME device. The colorant undergoes a color change of Delta E (ΔE) of equal to or greater than 3 units upon exposure to moisture but not upon exposure to high humidity. The colorant is visually obvious to the unaided human eye under normal light conditions through the body of the device. The HME device with the visual indicator for moisture can indicate to the care-giver that it is time to change the HME device.

摘要翻译： 提供了用于热和湿气交换（HME）装置的湿度指示器，其使用预着色剂和施加到HME装置的内表面的活化剂。 着色剂在暴露于水分但不暴露于高湿度时，经历Delta E（＆Dgr; E）的颜色变化等于或大于3单位。 着色剂在通常的光照条件下通过装置的主体在肉眼上视觉上是显而易见的。 具有湿度视觉指示器的HME设备可以向护理人员显示是否更换HME设备的时间。