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1.发明申请Whole cell engineering by mutagenizing a substantial portion of a starting genome combining mutations and optionally repeating 审中-公开通过诱变大部分起始基因组合突变和任选地重复的整个细胞工程公开(公告)号：US20050124010A1
公开(公告)日：2005-06-09
申请号：US10398271
申请日：2001-10-01
申请人： Jay Short , Pengcheng Fu , Jing Wei , Michael Levin , Martin Latterich
发明人： Jay Short , Pengcheng Fu , Jing Wei , Michael Levin , Martin Latterich
IPC分类号： A01H1/00 , A01K67/027 , C12N20060101 , C12N15/00 , C12N15/09 , C12N15/10 , C12N15/82 , C12Q1/02 , C12Q1/68 , G01N27/447 , G01N27/62 , G01N30/88 , G01N33/483 , G01N33/53 , G01N33/534 , G01N33/566 , G01N33/58 , G01N33/68 , G01N37/00 , G01N33/574 , G01N33/48 , G01N33/50 , G06F19/00
CPC分类号： C12N15/102 , C12N15/1027 , C12N15/1058 , C12N15/8241 , G01N33/534 , G01N33/6848 , G01N2800/52
摘要： This invention relates to the field of cellular and whole organism engineering. Specifically, this invention relates to a cellular transformation, directed evolution, and screening method for creating novel transgenic organisms having desirable properties. Thus in one aspect, this invention relates to a method of generating a transgenic organism, such as a microbe or a plant, having a plurality of traits that are diffenentially activatable.
摘要翻译： 本发明涉及细胞和整体生物工程领域。 具体地，本发明涉及用于产生具有期望性质的新型转基因生物的细胞转化，定向进化和筛选方法。 因此，在一个方面，本发明涉及产生具有多个难以发生活性的性状的转基因生物体如微生物或植物的方法。
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公开(公告)号：US20050202426A1
公开(公告)日：2005-09-15
申请号：US10491358
申请日：2002-10-01
申请人： Jay Short , Martin Latterich , Michael Levin , Jing Wei , Pengcheng Fu
发明人： Jay Short , Martin Latterich , Michael Levin , Jing Wei , Pengcheng Fu
IPC分类号： G01N27/62 , A61K48/00 , B01J20/281 , B01J20/285 , C07K1/16 , C12N1/15 , C12N1/21 , C12N5/10 , C12N15/09 , C12N15/85 , C12Q1/00 , C12Q1/02 , C12Q1/68 , G01N27/64 , G01N30/04 , G01N30/06 , G01N30/26 , G01N30/34 , G01N30/46 , G01N30/60 , G01N30/72 , G01N30/84 , G01N30/86 , G01N30/88 , G01N31/00 , G01N31/22 , G01N33/48 , G01N33/50 , G01N33/569 , G01N33/58 , G01N33/68 , G06F19/00
CPC分类号： G01N33/56911 , G01N33/5091
摘要： The invention provides methods for whole cell engineering of new and modified phenotypes by using “on-line” or “real-time” metabolic flux analysis. The invention provides a method for whole cell engineering of new or modified phenotypes by using real-time metabolic flux analysis by making a modified cell by modifying the genetic composition of a cell and culturing the modified cell to generate a plurality of modified cells and measuring at least one metabolic parameter of the cell by monitoring the cell culture of in real time. The invention also provides articles comprising machine-readable medium including machine-executable instructions and systems, e.g., computer systems, to practice the methods of the invention.
摘要翻译： 本发明通过使用“在线”或“实时”代谢通量分析提供新的和修饰的表型的全细胞工程的方法。 本发明提供了一种通过使用实时代谢通量分析通过改变细胞的遗传组成并培养修饰的细胞以产生多个修饰的细胞并通过在 通过实时监测细胞培养物，细胞的至少一种代谢参数。 本发明还提供包括机器可读介质的文章，其包括机器可执行指令和系统，例如计算机系统，以实施本发明的方法。
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公开(公告)号：US20100280803A1
公开(公告)日：2010-11-04
申请号：US12769555
申请日：2010-04-28
CPC分类号： G06F19/12 , G06F19/18 , Y02A90/26 , Y02E50/343
摘要： The invention provides an in silico model for determining a S. cerevisiae physiological function. The model includes a data structure relating a plurality of S. cerevisiae reactants to a plurality of S. cerevisiae reactions, a constraint set for the plurality of S. cerevisiae reactions, and commands for determining a distribution of flux through the reactions that is predictive of a S. cerevisiae physiological function. A model of the invention can further include a gene database containing information characterizing the associated gene or genes. The invention further provides methods for making an in silico S. cerevisiae model and methods for determining a S. cerevisiae physiological function using a model of the invention.
摘要翻译： 本发明提供了一种用于确定酿酒酵母生理功能的计算机模型。 该模型包括将多个酿酒酵母反应物与多个酿酒酵母反应相关联的数据结构，针对多个酿酒酵母反应的约束集，以及用于确定通过预测的反应的通量分布的命令 酿酒酵母的生理功能。 本发明的模型还可以包括含有表征相关基因或基因的信息的基因数据库。 本发明进一步提供了使用本发明的模型制备计算机酿酒酵母模型和确定酿酒酵母生理功能的方法。
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4.发明授权公开(公告)号：US08170852B2
公开(公告)日：2012-05-01
申请号：US12769555
申请日：2010-04-28
CPC分类号： G06F19/12 , G06F19/18 , Y02A90/26 , Y02E50/343
摘要： The invention provides an in silico model for determining a S. cerevisiae physiological function. The model includes a data structure relating a plurality of S. cerevisiae reactants to a plurality of S. cerevisiae reactions, a constraint set for the plurality of S. cerevisiae reactions, and commands for determining a distribution of flux through the reactions that is predictive of a S. cerevisiae physiological function. A model of the invention can further include a gene database containing information characterizing the associated gene or genes. The invention further provides methods for making an in silico S. cerevisiae model and methods for determining a S. cerevisiae physiological function using a model of the invention.
摘要翻译： 本发明提供了一种用于确定酿酒酵母生理功能的计算机模型。 该模型包括将多个酿酒酵母反应物与多个酿酒酵母反应相关联的数据结构，针对多个酿酒酵母反应的约束集，以及用于确定通过预测的反应的通量分布的命令 酿酒酵母的生理功能。 本发明的模型还可以包括含有表征相关基因或基因的信息的基因数据库。 本发明进一步提供了使用本发明的模型制备计算机酿酒酵母模型和确定酿酒酵母生理功能的方法。
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5.发明申请METHODS AND COMPOSITIONS FOR GENETIC ENGINEERING OF CYANOBACTERIA TO PRODUCE ETHANOL 审中-公开用于生产乙醇的氰尿酸的遗传工程的方法和组合物公开(公告)号：US20120045821A1
公开(公告)日：2012-02-23
申请号：US13148469
申请日：2009-02-12
申请人： Pengcheng Fu
发明人： Pengcheng Fu
CPC分类号： C12N9/88 , C12P7/065 , C12Y401/01001 , Y02E50/17
摘要： Provided herein are compositions and methods for genetic engineering of cyanobacteria to produce ethanol. In one aspect, the present invention provides a polynucleotide construct comprising a copper ion inductive promoter and a sequence encoding a pyruvate decarboxylase (pdc) enzyme. In another aspect, the present invention provides a genetically engineered cyanobacterium comprising the polynucleotide construct of the invention, wherein the cyanobacterium is capable of producing ethanol after a period of fermentation. In yet another aspect, the present invention discloses a method of producing ethanol by genetically modifying cyanobacteria using the polynucleotide construct of the invention.
摘要翻译： 本文提供了用于生产乙醇的蓝细菌遗传工程的组合物和方法。 一方面，本发明提供了包含铜离子诱导启动子和编码丙酮酸脱羧酶（pdc）酶的序列的多核苷酸构建体。 在另一方面，本发明提供了包含本发明的多核苷酸构建体的遗传工程化蓝细菌，其中蓝细菌在发酵期后能够产生乙醇。 在另一方面，本发明公开了使用本发明的多核苷酸构建体通过遗传修饰蓝细菌来生产乙醇的方法。
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6.发明授权公开(公告)号：US07751981B2
公开(公告)日：2010-07-06
申请号：US10263901
申请日：2002-10-02
CPC分类号： G06F19/12 , G06F19/18 , Y02A90/26 , Y02E50/343
摘要： The invention provides an in silico model for determining a S. cerevisiae physiological function. The model includes a data structure relating a plurality of S. cerevisiae reactants to a plurality of S. cerevisiae reactions, a constraint set for the plurality of S. cerevisiae reactions, and commands for determining a distribution of flux through the reactions that is predictive of a S. cerevisiae physiological function. A model of the invention can further include a gene database containing information characterizing the associated gene or genes. The invention further provides methods for making an in silico S. cerevisiae model and methods for determining a S. cerevisiae physiological function using a model of the invention.
摘要翻译： 本发明提供了一种用于确定酿酒酵母生理功能的计算机模型。 该模型包括将多个酿酒酵母反应物与多个酿酒酵母反应相关联的数据结构，针对多个酿酒酵母反应的约束集，以及用于确定通过预测的反应的通量分布的命令 酿酒酵母的生理功能。 本发明的模型还可以包括含有表征相关基因或基因的信息的基因数据库。 本发明进一步提供了使用本发明的模型制备计算机酿酒酵母模型和确定酿酒酵母生理功能的方法。
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