公开(公告)号：US06858435B2

公开(公告)日：2005-02-22

申请号：US09970544

申请日：2001-10-03

申请人： Jean-Pierre Chervet ,  Remco Swart ,  Johannes Petrus Cornelus Vissers

发明人： Jean-Pierre Chervet ,  Remco Swart ,  Johannes Petrus Cornelus Vissers

IPC分类号： G01N27/62 ,  G01N30/02 ,  G01N30/26 ,  G01N30/32 ,  G01N30/34 ,  G01N30/72 ,  G01N30/78 ,  G01N30/80 ,  G01N30/84 ,  G01N30/86 ,  G01N30/88

CPC分类号： G01N30/84 ,  G01N30/32 ,  G01N30/7233 ,  G01N30/728 ,  G01N30/8631 ,  G01N2030/324 ,  G01N2030/8411 ,  Y10T436/24 ,  Y10T436/255

摘要： A reconfigurable multi-mode post-column analysis system improves resolution of liquid chromatography detection data by providing a parking or MS/MS detection mode in which flow of column eluent is substantially reduced to provide greater analysis time within a single eluting detection peak. A micro switching valve unit is reconfigurable between normal (MS) mode that passes column eluent directly to an MS unit, and a peak parking (or MS/MS) mode, in which normal mode column flow is interrupted, and the gradient halted, while more slowly flowing the detection peak of interest through the MS unit. A micro syringe pump contributes to the substantially lower MS/MS flow rate. A MS control unit controls micro switching valve valves to reconfigure between MS and MS/MS modes. After a detection peak, MS mode is reestablished, and the system primary pump returns to normal mode flow rate and gradient conditions. Mode changes can be carried out for each chromatogram detection peak.

摘要翻译： 可重新配置的多模式后柱分析系统通过提供停放或MS / MS检测模式来提高液相色谱检测数据的分辨率，其中柱洗脱液的流量被显着减少，以在单个洗脱检测峰内提供更大的分析时间。 微型切换阀单元可以在通过柱洗脱液直接送到MS单元的正常（MS）模式和在其中正常模式列流中断的峰值驻留（或MS / MS）模式下重新配置，并且梯度停止，同时 通过MS单元更慢地流过感兴趣的检测峰。 微注射泵有助于实质上较低的MS / MS流速。 MS控制单元控制微动开关阀阀门在MS和MS / MS模式之间进行重新配置。 检测峰值后，重新建立MS模式，系统主泵返回正常模式的流量和梯度条件。 可以对每个色谱图检测峰进行模式更改。