公开(公告)号：US5223131A

公开(公告)日：1993-06-29

申请号：US773178

申请日：1991-10-08

申请人： James A. Apffel, Jr. ,  Robert G. Nordman ,  Mirko Martich

发明人： James A. Apffel, Jr. ,  Robert G. Nordman ,  Mirko Martich

IPC分类号： G01N27/62 ,  G01N30/72 ,  H01J49/04

CPC分类号： G01N30/728 ,  H01J49/04 ,  G01N30/7273

摘要： Close coupled momentum separator with integral removable source volume for particle beam liquid chromatography/mass spectrometry is provided. The inventive PB LC/MS system comprises a momentum separator wherein the skimmers are designed to accommodate the particle beam conical dispersion so as to minimize the number of particles lost in the separator. In addition, the exit of the momentum separator is closely coupled to the entrance of the MS source volume. The PB LC/MS system demonstrates improved linearity in signal and enhanced sensitivity.

公开(公告)号：US5117109A

公开(公告)日：1992-05-26

申请号：US578267

申请日：1990-09-06

申请人： Naoki Asakawa ,  Hiroshi Ohe ,  Yutaka Yoshida ,  Tadashi Sato ,  Yukuo Nezu ,  Yoshiya Oda

发明人： Naoki Asakawa ,  Hiroshi Ohe ,  Yutaka Yoshida ,  Tadashi Sato ,  Yukuo Nezu ,  Yoshiya Oda

IPC分类号： G01N27/62 ,  G01N30/26 ,  G01N30/72 ,  G01N30/80 ,  G01N30/84

CPC分类号： G01N30/728 ,  G01N30/80 ,  G01N2030/8411

摘要： The components present in the sample with a mobile phase A in 1st section is separated. In 2nd section there is provided with an inserting line of a dilute solution for diluting the mobile phase A and a trapping column which forces the dilute solution to pass but catches the components by connecting said line to said column. In 3rd section, the components caught by the trapping column are introduced into mass spectrometer by using a mobile phase B. It is preferable to use respectively different mobile phases for the mobile phase A and the mobile phase B. It is good to install separating columns in 1st and 3rd sections. It is also desired to add the retaining apparatus of the components separated in 1st section.

摘要翻译： 第一部分中流动相A的样品中存在的组分被分离。 在第二部分中，设置有用于稀释流动相A的稀释溶液的插入线和迫使稀释溶液通过的捕集柱，但是通过将所述管线连接到所述柱而捕获部件。 在第3部分中，捕集塔捕获的成分通过使用流动相B引入质谱仪。优选在流动相A和流动相B中分别使用不同的流动相。安装分离塔是很好的 在第1和第3部分。 还希望添加在第一部分中分离的部件的保持装置。

公开(公告)号：US3507147A

公开(公告)日：1970-04-21

申请号：US3507147D

申请日：1967-08-21

申请人： VARIAN ASSOCIATES

发明人： LLEWELLYN PETER M

IPC分类号： G01N1/00 ,  G01N30/06 ,  G01N30/72 ,  G01N30/84 ,  G01N31/08 ,  G01N1/22

CPC分类号： G01N30/728 ,  G01N30/06 ,  G01N2030/8411 ,  G01N2030/8417

公开(公告)号：US06858435B2

公开(公告)日：2005-02-22

申请号：US09970544

申请日：2001-10-03

申请人： Jean-Pierre Chervet ,  Remco Swart ,  Johannes Petrus Cornelus Vissers

发明人： Jean-Pierre Chervet ,  Remco Swart ,  Johannes Petrus Cornelus Vissers

IPC分类号： G01N27/62 ,  G01N30/02 ,  G01N30/26 ,  G01N30/32 ,  G01N30/34 ,  G01N30/72 ,  G01N30/78 ,  G01N30/80 ,  G01N30/84 ,  G01N30/86 ,  G01N30/88

CPC分类号： G01N30/84 ,  G01N30/32 ,  G01N30/7233 ,  G01N30/728 ,  G01N30/8631 ,  G01N2030/324 ,  G01N2030/8411 ,  Y10T436/24 ,  Y10T436/255

摘要： A reconfigurable multi-mode post-column analysis system improves resolution of liquid chromatography detection data by providing a parking or MS/MS detection mode in which flow of column eluent is substantially reduced to provide greater analysis time within a single eluting detection peak. A micro switching valve unit is reconfigurable between normal (MS) mode that passes column eluent directly to an MS unit, and a peak parking (or MS/MS) mode, in which normal mode column flow is interrupted, and the gradient halted, while more slowly flowing the detection peak of interest through the MS unit. A micro syringe pump contributes to the substantially lower MS/MS flow rate. A MS control unit controls micro switching valve valves to reconfigure between MS and MS/MS modes. After a detection peak, MS mode is reestablished, and the system primary pump returns to normal mode flow rate and gradient conditions. Mode changes can be carried out for each chromatogram detection peak.

摘要翻译： 可重新配置的多模式后柱分析系统通过提供停放或MS / MS检测模式来提高液相色谱检测数据的分辨率，其中柱洗脱液的流量被显着减少，以在单个洗脱检测峰内提供更大的分析时间。 微型切换阀单元可以在通过柱洗脱液直接送到MS单元的正常（MS）模式和在其中正常模式列流中断的峰值驻留（或MS / MS）模式下重新配置，并且梯度停止，同时 通过MS单元更慢地流过感兴趣的检测峰。 微注射泵有助于实质上较低的MS / MS流速。 MS控制单元控制微动开关阀阀门在MS和MS / MS模式之间进行重新配置。 检测峰值后，重新建立MS模式，系统主泵返回正常模式的流量和梯度条件。 可以对每个色谱图检测峰进行模式更改。

公开(公告)号：US5481107A

公开(公告)日：1996-01-02

申请号：US302555

申请日：1994-09-08

申请人： Yasuaki Takada ,  Minoru Sakairi ,  Atsumu Hirabayashi ,  Youichi Ose

发明人： Yasuaki Takada ,  Minoru Sakairi ,  Atsumu Hirabayashi ,  Youichi Ose

IPC分类号： G01N27/62 ,  G01N30/72 ,  H01J49/04 ,  H01J49/06 ,  H01J49/22 ,  H01J49/40 ,  H01J49/42

CPC分类号： H01J49/044 ,  G01N30/728 ,  H01J49/061 ,  G01N30/7266 ,  G01N30/7293

摘要： A mass spectrometer comprising an ionization region for ionizing a sample under atmospheric pressure, an ion sampling aperture for introducing ions generated by the ionization region into a vacuum, and a mass analysis region for mass analyzing the ions on the basis of a high-frequency electric field, wherein: an electrostatic lens for deflecting the direction of the movement of the ion from the center axis of the ion sampling aperture is disposed between the ionization region and the mass analysis region; the center axis of an aperture for introducing ions into the mass analysis region and the center axis of the ion sampling aperture are disposed so as to be shifted in parallel from each other; and the center axis of the ion sampling aperture and the center axis of a cylindrical inner electrode constituting the electrostatic lens are disposed so as to be shifted in parallel from each other to thereby prevent charged droplets or droplets without charge from flowing into the mass analysis region.

摘要翻译： 一种质谱仪，其特征在于，包括用于在大气压下使样品离子化的电离区域，用于将由离子化区域产生的离子引入真空的离子取样孔，以及质量分析区域，用于基于高频电力 其特征在于，在所述电离区域和所述质量分析区域之间配置用于使所述离子从所述离子取样孔的中心轴线移动的方向偏转的静电透镜; 用于将离子引入质量分析区域和离子采样孔的中心轴的孔的中心轴线彼此平行地移位; 并且离子采样孔的中心轴线和构成静电透镜的圆柱形内部电极的中心轴线彼此平行地移位，从而防止带电液滴或液滴而不带电流入质量分析区域 。

公开(公告)号：US20020072126A1

公开(公告)日：2002-06-13

申请号：US09970544

申请日：2001-10-03

申请人： DIONEX CORPORATION

发明人： Jean-Pierre Chervet ,  Remco Swart ,  Johannes Petrus Cornelus Vissers

IPC分类号： G01N030/88

CPC分类号： G01N30/84 ,  G01N30/32 ,  G01N30/7233 ,  G01N30/728 ,  G01N30/8631 ,  G01N2030/324 ,  G01N2030/8411 ,  Y10T436/24 ,  Y10T436/255

摘要： A reconfigurable multi-mode post-column analysis system improves resolution of liquid chromatography detection data by providing a parking or MS/MS detection mode in which flow of column eluent is substantially reduced to provide greater analysis time within a single eluting detection peak. A micro switching valve unit is reconfigurable between normal (MS) mode that passes column eluent directly to an MS unit, and a peak parking (or MS/MS) mode, in which normal mode column flow is interrupted, and the gradient halted, while more slowly flowing the detection peak of interest through the MS unit. A micro syringe pump contributes to the substantially lower MS/MS flow rate. A MS control unit controls micro switching valve valves to reconfigure between MS and MS/MS modes. After a detection peak, MS mode is reestablished, and the system primary pump returns to normal mode flow rate and gradient conditions. Mode changes can be carried out for each chromatogram detection peak.

摘要翻译： 可重新配置的多模式后柱分析系统通过提供停放或MS / MS检测模式来提高液相色谱检测数据的分辨率，其中柱洗脱液的流量被显着减少，以在单个洗脱检测峰内提供更大的分析时间。 微型切换阀单元可以在通过柱洗脱液直接送到MS单元的正常（MS）模式和在其中正常模式列流中断的峰值驻留（或MS / MS）模式下重新配置，并且梯度停止，同时 通过MS单元更慢地流过感兴趣的检测峰。 微注射泵有助于实质上较低的MS / MS流速。 MS控制单元控制微动开关阀阀门在MS和MS / MS模式之间进行重新配置。 检测峰值后，重新建立MS模式，系统主泵返回正常模式的流量和梯度条件。 可以对每个色谱图检测峰进行模式更改。

公开(公告)号：US5449902A

公开(公告)日：1995-09-12

申请号：US168884

申请日：1993-12-16

申请人： Kouzi Onishi ,  Norio Tada ,  Yoshinobu Yoshimura ,  Yoshiaki Kato

发明人： Kouzi Onishi ,  Norio Tada ,  Yoshinobu Yoshimura ,  Yoshiaki Kato

IPC分类号： G01N30/02 ,  G01N30/72 ,  G01N30/84 ,  G01N35/08 ,  H01J49/04

CPC分类号： H01J49/0431 ,  G01N30/728 ,  G01N35/085 ,  G01N2030/027 ,  G01N2030/8411

摘要： An apparatus for directly connecting an analytical column and a mass spectrometer comprising a fixed member having at least four holes which respectively introduce washing solution, eluate containing a component eluted from the analytical column, desalting solution and eluent for eluting the component, and a movable member rotated with respect to an axis having at least four tubes around the axis and mounting the four trapping columns, whereby the trapping columns are respectively washed, trapped, desalted and eluted in parallel. Furthermore, a common trapping column may be used instead of the four trapping columns by controlling the apparatus with four analytical modes.

摘要翻译： 一种用于直接连接分析柱和质谱仪的装置，包括具有至少四个孔的固定构件，其分别引入洗涤溶液，含有从分析柱洗脱的组分的洗脱液，脱盐溶液和用于洗脱组分的洗脱液;以及可移动构件 相对于围绕轴线具有至少四个管的轴线旋转并安装四个捕集柱，由此分别洗涤，捕获，脱盐和洗脱平行的捕集柱。 此外，通过使用四种分析模式控制该装置，可以使用公共捕集塔来代替四个捕集塔。

公开(公告)号：US4713963A

公开(公告)日：1987-12-22

申请号：US879022

申请日：1986-06-26

申请人： Daryl Sharp

发明人： Daryl Sharp

IPC分类号： G01N30/16 ,  G01N30/72 ,  G01N31/08

CPC分类号： G01N30/7206 ,  G01N2030/165 ,  G01N2030/7226 ,  G01N30/728

摘要： A method and device for analyzing samples containing mixtures of organic compounds using vacuum chromatography is disclosed. The method and device are particularly useful for obtaining mass spectra for identification purposes. The method involves passing a sample dissolved in a solvent through a column with a carrier gas and depositing a thin layer of the sample in the interior of a short column. The short column is then attached to an MS interface. The device comprises a combination of a variable molecular separator and a column alignment component. The alignment component may also be used as a direct probe injector. This device and method allow the separation and MS analysis of mixtures of intermediate molecular weight compounds.

摘要翻译： 公开了一种使用真空色谱分析含有有机化合物混合物的样品的方法和装置。 该方法和装置对于获得用于识别目的的质谱特别有用。 该方法包括将溶解在溶剂中的样品通过载体载气柱并将样品的薄层沉积在短柱的内部。 然后将该短列连接到MS接口。 该装置包括可变分子分离器和柱对准组分的组合。 对准部件也可以用作直接探针喷射器。 该装置和方法允许中等分子量化合物的混合物的分离和MS分析。

公开(公告)号：US20060110833A1

公开(公告)日：2006-05-25

申请号：US11223456

申请日：2005-09-09

申请人： George Agnes ,  Michael Bogan

发明人： George Agnes ,  Michael Bogan

IPC分类号： G01N33/00 ,  B01D59/44 ,  H01J49/00

CPC分类号： B01L3/0268 ,  B01L2200/0626 ,  B01L2400/0415 ,  G01N30/7233 ,  G01N30/728 ,  H01J49/0431 ,  H01J49/164

摘要： This application relates to a method and apparatus for coupling an analyte supply, such as a biomolecule separator, to an electrodynamic droplet processor. In one embodiment the biomolecule separator is a capillary liquid chromatography column and the droplet processor includes an droplet generator and an electrodynamic balance. The biomolecule separator and the droplet processor may be fluidly coupled to provide a continuous supply of analyte for analysis. The droplets may be controllably ejected from the electrodynamic balance and deposited on a target substrate for use in detecting the analyte by mass spectrometry, such as MALDI time of flight mass spectrometry. Prior to deposition, each of the droplets is levitated in the electrodynamic balance for a period sufficient to enable evaporation of volatile solvents present in the droplet solution, thereby increasing the analyte concentration in the droplet. The solution may include a MALDI liquid matrix and the target substrate may be a MALDI plate. In one embodiment, the method involves depositing a succession of discrete droplets on the target substrate to form one or more microspots having a high density of analytes. The microspots are then irradiated and the ions produced are analyzed by mass spectrometry. The invention improves the sensitivity of analyte detection while consuming a comparatively small volume of test solution.

摘要翻译： 本申请涉及用于将分析物供应（例如生物分子分离器）耦合到电动液滴处理器的方法和装置。 在一个实施方案中，生物分子分离器是毛细管液相色谱柱，液滴处理器包括液滴发生器和电动余量。 生物分子分离器和液滴处理器可以流体耦合以提供用于分析的分析物的连续供应。 液滴可以可控地从电动余量喷出并沉积在靶基质上，用于通过质谱法（如MALDI飞行时间质谱法）检测分析物。 在沉积之前，每个液滴在电动余量中悬浮一段足以能够蒸发存在于液滴溶液中的挥发性溶剂，从而增加液滴中的分析物浓度。 溶液可以包括MALDI液体基质，靶基质可以是MALDI板。 在一个实施方案中，该方法包括在目标基底上沉积一系列离散液滴以形成具有高密度分析物的一个或多个微点。 然后照射微孢子，通过质谱法分析产生的离子。 本发明在消耗相对小体积的测试溶液的同时提高了分析物检测的灵敏度。

公开(公告)号：US20040023410A1

公开(公告)日：2004-02-05

申请号：US10213144

申请日：2002-08-05

发明人： Catherine Stacey

IPC分类号： G01N024/00

CPC分类号： H01J49/0418 ,  G01N30/728 ,  Y10T436/24

摘要： Disclosed is a method and apparatus for matrix-assisted laser desorption ionization (MALDI), whereby the components of a sample separated by the column of a chromatographic separation device such as a liquid chromatograph or capillary electrophoresis are eluted and deposited on a sample support plate in a continuous track which both concentrates the sample components and preserves the fidelity of the separation. Analysis by MALDI is thus decoupled from the requirements of the separation and deposition and is performed by moving the continuous track relative to a focused laser beam in order to ionize the sample. The deposition capillary is in relative motion to the sample support plate with a speed of motion compatible with the liquid flow rate. In order to prevent the liquid sample from spreading across the sample support plate and to focus it to a pre-defined narrow region, the deposition of the sample is made along a hydrophilic anchor track of a well-defined width of less than 1 mm on the surface of the sample plate.

摘要翻译： 公开了一种用于基质辅助激光解吸电离（MALDI）的方法和装置，其中由诸如液相色谱仪或毛细管电泳的色谱分离装置的柱分离的样品的组分被洗脱并沉积在样品支持板上 这是一个连续的轨道，既可以集中样品组分，又能保持分离的保真度。 因此，MALDI的分析与分离和沉积的要求分离，并且通过相对于聚焦的激光束移动连续轨迹来进行，以使样品电离。 沉积毛细管与样品支撑板相对运动，其运动速度与液体流速相容。 为了防止液体样品扩散穿过样品支撑板并将其聚焦到预定义的窄区域，样品的沉积沿着具有小于1mm的良好限定宽度的亲水性锚定轨迹 样品板的表面。